Standard name
Human Ortholog
Description Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.75 0.66 0.9 0.9 0.73 0.77 0.68 – 0.61 0.59 0.47 0.57 0.73 0.85 0.95 0.87 0.87 0.89 0.5 0.43 0.52 0.67 0.72 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.17 0.22 – 0.38 0.4 0.5 0.31 0.07 0 0 0.06 0 0.05 0.07 0.05 0.09 0.11 0.1 0.1
Nucleus 0.11 0.19 0.05 0.06 0.19 0.1 0.15 – 0.12 0.15 0.16 0.15 0.12 0 0 0 0 0 0.17 0.2 0.11 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0
Vac/Vac Membrane 0.1 0.15 0 0 0.05 0 0 – 0 0 0 0 0.12 0.16 0 0.09 0.08 0.08 0.13 0.15 0.12 0.09 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 3 5 7 9
Bud 2 0 1 2 3 0 9 – 14 14 14 4 5 0 2 0 0 0 1 6 4 4 12 14
Bud Neck 1 0 10 9 15 7 22 – 16 8 2 11 0 0 0 0 0 2 1 1 2 4 4 6
Bud Site 0 0 0 0 0 0 4 – 3 4 8 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 3 1 1 0 – 0 0 0 1 0 0 0 4 1 4 0 0 2 3 4 9
Cytoplasm 171 153 349 304 334 401 414 – 344 302 207 265 270 476 606 265 224 222 137 108 222 211 266 381
Endoplasmic Reticulum 0 5 1 1 1 1 0 – 0 0 0 0 0 0 0 20 10 17 1 1 1 4 5 3
Endosome 1 5 1 0 0 1 2 – 1 0 0 1 6 10 2 7 3 3 3 5 3 1 1 1
Golgi 0 1 1 0 0 0 0 – 0 0 0 0 0 1 0 1 0 1 0 0 1 1 3 3
Mitochondria 22 8 2 11 15 89 134 – 211 205 222 145 24 5 10 19 9 12 18 11 39 33 36 49
Nucleus 25 45 21 20 87 54 94 – 67 76 72 71 43 4 1 11 9 5 45 51 45 4 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 – 1 0 0 1 1 1 0 0 0 0 2 1 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1
SpindlePole 1 1 0 3 4 9 18 – 7 3 4 10 0 1 0 0 2 0 12 16 27 4 2 8
Vac/Vac Membrane 24 34 11 4 24 6 7 – 6 7 14 6 45 88 26 27 20 21 35 37 50 26 13 11
Unique Cell Count 229 231 388 339 457 520 609 562 508 444 464 369 558 636 306 258 249 274 250 431 313 369 509
Labelled Cell Count 247 252 402 357 484 569 704 670 619 543 520 394 587 648 355 278 287 274 250 431 313 369 509


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.3 4.7 4.1 4.9 3.5 3.9 – 3.3 3.2 3.1 3.7 4.2 4.0 3.8 5.3 5.6 5.1 4.0 3.9 4.0
Std Deviation (1e-4) 0.5 1.0 0.8 0.9 1.4 0.7 1.0 – 0.7 0.7 0.7 1.1 2.2 1.1 1.2 1.2 1.5 1.1 2.7 1.8 1.5
Intensity Change (Log2) – – – -0.22 0.06 -0.41 -0.29 – -0.52 -0.56 -0.6 -0.36 -0.17 -0.25 -0.31 0.18 0.24 0.11 -0.26 -0.28 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.4 2.9 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7867 -0.5585 -0.4493 -0.6294 -0.685 -0.6288 -0.1975 0.0034 0.0847 -0.6444 -0.2375 -0.3366 -0.8427 -0.5027 -0.6811 -0.6051 -0.3686 -0.9135
Actin 0.0162 0.0008 0.0041 0.0004 0.0032 0.0069 0.0249 0.0004 0.0017 0.0006 0.0153 0.0069 0.0282 0.0006 0.0064 0.0086 0.0169 0.001
Bud 0.0069 0.0042 0.0033 0.0026 0.0015 0.0005 0.0044 0.0012 0.0016 0.0085 0.004 0.0013 0.0014 0.0006 0.0003 0.0016 0.0028 0.0002
Bud Neck 0.0036 0.0005 0.001 0.0004 0.0011 0.0014 0.0061 0.0006 0.0012 0.0015 0.0008 0.0053 0.001 0.0004 0.0005 0.0031 0.0021 0.0017
Bud Periphery 0.0066 0.0023 0.0017 0.0004 0.0022 0.0008 0.0059 0.0019 0.0031 0.0101 0.0192 0.0012 0.002 0.0016 0.0002 0.0037 0.0041 0.0003
Bud Site 0.01 0.0029 0.0049 0.0007 0.0005 0.0004 0.0187 0.0035 0.0046 0.0039 0.001 0.0061 0.0024 0.0031 0.0024 0.0046 0.0155 0.0002
Cell Periphery 0.0005 0.0001 0.0002 0.0005 0.0001 0.0001 0.0006 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0003 0.0004 0
Cytoplasm 0.1072 0.1166 0.0845 0.127 0.1025 0.1104 0.168 0.1724 0.1486 0.2206 0.2055 0.2648 0.1651 0.2063 0.1862 0.1137 0.0621 0.2585
Cytoplasmic Foci 0.0484 0.0124 0.0106 0.0229 0.0379 0.0179 0.0338 0.007 0.007 0.0246 0.0122 0.0126 0.0288 0.004 0.0105 0.0077 0.0227 0.0078
Eisosomes 0.0005 0.0001 0.0002 0.0008 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0065 0.003 0.0031 0.0016 0.0083 0.0034 0.0072 0.0028 0.0067 0.0046 0.0039 0.0031 0.0083 0.0021 0.0033 0.0031 0.0031 0.002
Endosome 0.0268 0.005 0.0145 0.0407 0.0327 0.0154 0.027 0.0032 0.0124 0.0053 0.0241 0.0056 0.0333 0.002 0.0126 0.0099 0.0414 0.0063
Golgi 0.0081 0.0003 0.0046 0.0072 0.0037 0.0084 0.0065 0.0002 0.0013 0.0006 0.0046 0.003 0.0091 0.0001 0.0039 0.0079 0.0289 0.0011
Lipid Particles 0.0223 0.0011 0.0035 0.0076 0.007 0.0086 0.0132 0.0003 0.0015 0.0028 0.0145 0.0009 0.0144 0.0002 0.0025 0.0019 0.0251 0.0022
Mitochondria 0.0093 0.0018 0.0063 0.0188 0.0078 0.0209 0.0132 0.0008 0.0021 0.0036 0.0075 0.0029 0.0094 0.0007 0.0096 0.0066 0.017 0.0062
None 0.552 0.5368 0.4232 0.4651 0.5348 0.6277 0.5027 0.4495 0.256 0.3319 0.4257 0.3143 0.5414 0.4995 0.3421 0.4995 0.6403 0.5005
Nuclear Periphery 0.0197 0.0101 0.0227 0.0113 0.0206 0.0098 0.0158 0.009 0.0215 0.0156 0.008 0.0126 0.0153 0.0062 0.0081 0.0116 0.006 0.0056
Nucleolus 0.007 0.0057 0.0053 0.0251 0.0036 0.0028 0.0081 0.0038 0.0045 0.0033 0.0026 0.005 0.0035 0.0021 0.0021 0.004 0.0019 0.0016
Nucleus 0.1039 0.2633 0.3569 0.205 0.0969 0.121 0.0947 0.297 0.468 0.3292 0.2232 0.307 0.0886 0.2434 0.3921 0.271 0.081 0.1874
Peroxisomes 0.0084 0.0003 0.0025 0.0014 0.0026 0.0029 0.014 0.0003 0.0022 0.0025 0.0015 0.0005 0.0127 0.001 0.0018 0.001 0.0109 0.0006
Punctate Nuclear 0.0237 0.0297 0.0426 0.0557 0.1241 0.0361 0.0223 0.0416 0.0504 0.0264 0.0214 0.0442 0.022 0.0229 0.0134 0.0318 0.0092 0.0147
Vacuole 0.0099 0.0025 0.0031 0.0024 0.0047 0.0028 0.0098 0.0037 0.0036 0.0028 0.003 0.002 0.0094 0.0027 0.0016 0.0054 0.0065 0.0016
Vacuole Periphery 0.0024 0.0005 0.0013 0.0024 0.004 0.0016 0.0026 0.0004 0.0015 0.0014 0.0014 0.0005 0.0028 0.0003 0.0004 0.003 0.0019 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.8335 5.5182 1.7061 8.5729 12.3386 16.4519 5.1145 2.8243 6.7161 15.3425
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
281 570 1264 1394 1785 1080 162 1570 2066 1650 1426 2964

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.33 713.22 816.96 816.07 642.65 627.78 834.11 821.03 639.07 657.30 818.91 818.70
Standard Deviation 60.68 92.77 102.24 104.03 70.06 93.17 90.23 116.35 69.45 101.51 101.10 110.75
Intensity Change Log 2 — 0.210644 0.406562 0.404990 — -0.033774 0.376204 0.353402 — 0.091054 0.391146 0.378887

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000038 0.000268 0.000140 0.002002 0.000060 0.000316 0.000394 0.001135 0.000057 0.000299 0.000169 0.001543
Bud Neck 0.014507 0.021309 0.002899 0.009001 0.011114 0.035336 0.001180 0.011897 0.011576 0.030490 0.002703 0.010535
Bud Site 0.003439 0.008183 0.004556 0.050831 0.003264 0.009264 0.001413 0.038698 0.003288 0.008891 0.004199 0.044404
Cell Periphery 0.000272 0.000353 0.000310 0.000202 0.000120 0.000220 0.000232 0.000223 0.000141 0.000266 0.000301 0.000213
Cytoplasm 0.439679* 0.363131* 0.267325* 0.424908* 0.415937* 0.333948* 0.230288* 0.384772* 0.419166* 0.344029* 0.263117* 0.403648*
Cytoplasmic Foci 0.184179* 0.170884 0.013609 0.017310 0.145356 0.226610* 0.044357 0.009481 0.150637 0.207359* 0.017102 0.013163
Eisosomes 0.000179 0.000114 0.000064 0.000044 0.000085 0.000154 0.000137 0.000037 0.000098 0.000140 0.000072 0.000040
Endoplasmic Reticulum 0.001116 0.000891 0.001732 0.001981 0.000684 0.000805 0.002243 0.002096 0.000743 0.000835 0.001790 0.002042
Endosome 0.008375 0.009995 0.001506 0.008651 0.005139 0.020049 0.003834 0.004108 0.005579 0.016576 0.001770 0.006245
Golgi 0.001025 0.001624 0.000058 0.002932 0.000705 0.005564 0.000097 0.003621 0.000749 0.004203 0.000063 0.003297
Lipid Particles 0.013280 0.008597 0.001551 0.001144 0.007618 0.014323 0.004042 0.000922 0.008388 0.012345 0.001834 0.001026
Mitochondria 0.003205 0.012025 0.000521 0.004764 0.003546 0.026373 0.000694 0.002354 0.003500 0.021416 0.000540 0.003487
Mitotic Spindle 0.000535 0.010537 0.001867 0.062621 0.000880 0.009538 0.006007 0.030896 0.000833 0.009883 0.002337 0.045817
None 0.008223 0.011414 0.013983 0.009145 0.007136 0.013105 0.005109 0.006404 0.007284 0.012521 0.012975 0.007693
Nuclear Periphery 0.000577 0.000986 0.001313 0.002521 0.000820 0.000739 0.001238 0.001720 0.000787 0.000824 0.001304 0.002097
Nuclear Periphery Foci 0.000261 0.000569 0.000338 0.003122 0.000323 0.001054 0.000658 0.000655 0.000314 0.000887 0.000375 0.001815
Nucleolus 0.001828 0.004486 0.001014 0.000691 0.002125 0.006393 0.001472 0.000702 0.002085 0.005735 0.001066 0.000697
Nucleus 0.288616* 0.323657* 0.665561* 0.298507* 0.367942* 0.233796* 0.634257* 0.433718* 0.357153* 0.264839* 0.662004* 0.370127*
Peroxisomes 0.003208 0.012242 0.000219 0.002796 0.003862 0.019139 0.000956 0.000486 0.003773 0.016756 0.000302 0.001572
Vacuole 0.025594 0.037705 0.021266 0.092369 0.022845 0.040433 0.061126 0.063284 0.023219 0.039490 0.025794 0.076963
Vacuole Periphery 0.001865 0.001029 0.000170 0.004456 0.000436 0.002843 0.000266 0.002790 0.000630 0.002216 0.000181 0.003574

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.19 -7.46 -3.30 -2.21 -2.57 -5.30 -1.29 -2.35 -1.22 0.11 -6.89 -3.42 -3.88 -2.32 -2.54
Bud Neck -1.51 3.03 1.98 5.07 -4.99 -8.24 9.58 1.21 8.73 -7.47 -8.33 7.89 2.89 10.32 -6.79
Bud Site -2.33 -0.57 -5.85 -4.12 -5.63 -4.14 1.50 -7.07 -4.37 -7.98 -4.69 -0.68 -9.23 -5.95 -8.05
Cell Periphery -0.41 -0.15 3.54 1.36 4.79 -2.68 -5.27 -2.24 1.81 3.32 -1.95 -4.45 0.48 2.04 4.66
Cytoplasm 4.25 11.45 7.23 2.96 -7.26 7.95 11.52 12.24 2.41 -5.39 8.72 20.34 14.43 3.51 -8.68
Cytoplasmic Foci 0.80 14.89 15.03 18.55 1.06 -10.43 16.11 32.65 33.16 6.05 -8.67 33.00 35.54 37.45 5.39
Eisosomes 3.47 6.86 7.41 7.89 1.35 -9.22 -5.33 9.69 15.44 8.84 -6.98 4.35 11.23 17.01 5.13
Endoplasmic Reticulum 0.29 -3.48 -5.11 -14.07 -3.32 -1.08 -6.96 -16.20 -14.40 0.13 -1.07 -11.96 -19.33 -19.89 -3.16
Endosome -0.96 3.73 2.56 4.65 -3.32 -8.72 1.19 4.32 10.76 2.78 -8.69 6.42 4.27 11.48 -2.69
Golgi -1.58 3.79 0.62 2.32 -3.45 -6.83 5.20 -1.05 4.52 -2.08 -7.14 6.43 -0.98 5.33 -3.08
Lipid Particles 1.77 5.13 5.28 6.23 2.30 -4.65 2.20 13.06 9.89 3.19 -3.58 11.39 13.52 11.54 4.63
Mitochondria -2.94 1.57 0.71 4.12 -3.25 -8.66 4.97 3.40 9.61 -3.06 -9.12 5.72 3.07 10.36 -5.21
Mitotic Spindle -3.04 -2.03 -9.21 -4.89 -8.41 -4.17 -0.96 -7.30 -3.31 -3.93 -5.04 -1.83 -11.57 -6.09 -10.11
None -1.17 -1.51 0.79 2.21 2.98 -3.10 2.21 1.48 4.19 -1.18 -3.40 -2.29 1.26 4.62 3.35
Nuclear Periphery -2.18 -8.95 -14.21 -10.50 -10.17 0.86 -5.91 -18.17 -17.79 -7.78 -0.56 -11.38 -21.26 -18.96 -13.81
Nuclear Periphery Foci -3.25 -3.03 -3.85 -2.96 -3.25 -2.97 -3.67 -6.79 1.28 0.91 -3.45 -4.06 -4.96 -1.10 -3.70
Nucleolus -3.36 2.41 3.48 5.19 3.93 -4.84 2.43 4.49 7.92 2.80 -5.37 3.70 4.90 9.43 4.95
Nucleus -1.78 -22.22 -4.51 -2.93 28.45 12.85 -10.74 -12.01 -22.71 5.56 9.93 -32.87 -9.20 -18.72 26.01
Peroxisomes -5.01 4.41 3.15 6.69 -2.45 -9.69 2.83 9.83 12.34 1.27 -10.73 10.00 9.14 13.89 -1.24
Vacuole -3.49 -1.94 -25.23 -23.06 -25.86 -6.50 -6.77 -21.43 -16.64 -1.85 -7.75 -6.70 -33.99 -28.22 -27.03
Vacuole Periphery 0.88 1.76 0.59 -0.46 -2.27 -4.08 2.77 -2.03 2.35 -2.54 -3.85 3.27 -1.79 2.02 -3.49
Standard name
Human Ortholog
Description Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (16%), nucleus (13%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Esc8

Esc8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Esc8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available