Standard name
Human Ortholog
Description Subunit of the SMC5-SMC6 complex; the SMC5-SMC6 complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; supports nucleolar function; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.12 0.11 0.33 0.25 0.32 0.31 0.35 0.38 0.34 – 0.24 0.27 0.28 0.43 0.53 0.17 0.17 – 0.08 0.05 0.14 0.14 0.24 0.31
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0.22 0.35 0.59 0.3 0.66 0.53 0.62 0.71 – 0.77 0.81 0.06 0.06 0.22 0 0 – 0 0 0.08 0 0.05 0.05
Nucleus 0.78 0.6 0.31 0.36 0.26 0.16 0.23 0.17 0.17 – 0.16 0.14 0.55 0.36 0.2 0.43 0.56 – 0.58 0.65 0.3 0.39 0.23 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0.05 0.06 0.06 0 0
Vac/Vac Membrane 0.13 0.27 0.11 0 0.16 0.08 0.11 0.06 0.05 – 0.06 0.05 0.2 0.22 0.18 0.48 0.22 – 0.17 0.14 0.29 0.27 0.36 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 1 1 1 0 0 1
Bud 0 0 0 0 2 4 1 5 5 – 6 8 0 1 1 0 0 – 2 3 3 8 4 5
Bud Neck 0 0 1 0 2 1 0 0 0 – 1 0 0 1 0 0 0 – 0 0 0 0 3 2
Bud Site 0 0 0 0 3 2 3 3 7 – 5 7 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 – 0 1 0 1 0 0 0 – 0 0 0 0 0 0
Cytoplasm 13 25 49 32 75 80 103 109 106 – 82 109 65 110 139 4 3 – 11 13 28 28 53 65
Endoplasmic Reticulum 1 1 0 0 1 0 0 0 0 – 0 0 0 1 1 2 0 – 0 0 1 0 1 2
Endosome 0 2 1 0 17 2 4 1 2 – 0 0 0 6 1 1 1 – 4 4 6 3 1 8
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 1 0 1 1 0
Mitochondria 2 51 53 75 71 172 155 176 218 – 260 329 13 14 57 1 0 – 4 6 17 4 10 9
Nucleus 85 142 47 46 61 42 68 48 54 – 54 56 127 92 52 10 10 – 85 173 61 81 52 27
Nuclear Periphery 0 2 0 0 0 0 1 0 0 – 1 0 1 1 0 0 0 – 1 0 3 0 0 0
Nucleolus 1 2 0 0 8 2 1 1 2 – 2 3 0 0 0 0 0 – 2 4 2 2 1 2
Peroxisomes 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 1 11 1 1 2 9 – 2 7 0 1 0 0 0 – 5 13 13 13 9 9
Vac/Vac Membrane 14 63 16 5 39 21 31 16 14 – 20 21 46 55 47 11 4 – 24 38 58 56 81 71
Unique Cell Count 109 235 150 128 238 262 291 285 309 337 408 232 253 262 23 18 146 266 205 210 225 214
Labelled Cell Count 116 288 167 159 290 328 368 361 418 433 541 252 283 298 29 18 146 266 205 210 225 214


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.0 3.5 3.4 3.9 3.3 3.6 3.0 2.9 – 2.9 2.6 4.5 4.4 3.8 6.1 9.0 – 4.6 4.7 5.3
Std Deviation (1e-4) 0.8 0.6 0.8 1.0 1.8 1.1 2.0 1.1 1.2 – 1.0 0.8 1.1 1.1 1.0 1.2 3.6 – 1.6 2.1 2.4
Intensity Change (Log2) – – – -0.06 0.15 -0.09 0.04 -0.22 -0.3 – -0.3 -0.45 0.37 0.33 0.12 0.8 1.35 – 0.4 0.41 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9052 1.6955 1.8785 1.9636 1.5672 1.4339 2.4942 2.5995 2.6024 2.1891 3.0577 2.4297 2.8933 3.0126 3.294 3.3673 3.7181 2.8544
Actin 0.0846 0.0008 0.0132 0.0201 0.0169 0.0081 0.0493 0.0002 0.0026 0.0033 0.0069 0.0067 0.0224 0.0001 0.0006 0.0123 0.0361 0.0002
Bud 0.0023 0.0001 0.0003 0.0003 0.0005 0 0.0008 0.0001 0.0004 0.0001 0.0014 0.0002 0.0005 0.0001 0.0002 0.0001 0.0004 0
Bud Neck 0.0167 0.0006 0.0071 0.0024 0.0014 0.0036 0.0019 0.0091 0.0028 0.0024 0.0031 0.0032 0.0049 0.0006 0.0007 0.0009 0.0012 0.013
Bud Periphery 0.0048 0.0001 0.0007 0.0005 0.0006 0.0001 0.0011 0.0001 0.0007 0.0001 0.0022 0.0005 0.0008 0.0001 0.0004 0.0001 0.0004 0.0001
Bud Site 0.0122 0.0008 0.0031 0.002 0.0067 0.0001 0.0037 0.0041 0.0009 0.0003 0.004 0.0006 0.0084 0.0014 0.0005 0.0001 0.0029 0.0001
Cell Periphery 0.0009 0.0001 0.0003 0.0003 0.0002 0 0.0003 0.0001 0.0002 0 0.0003 0.0001 0.0005 0 0 0 0.0001 0
Cytoplasm 0.0236 0.0288 0.0067 0.0088 0.0039 0.0058 0.0366 0.0557 0.0167 0.0043 0.0139 0.0103 0.0467 0.0363 0.0075 0.01 0.0142 0.0158
Cytoplasmic Foci 0.0335 0.0013 0.0032 0.0393 0.028 0.0006 0.028 0.0007 0.0086 0.0059 0.0494 0.0075 0.0046 0.0005 0.0082 0.0066 0.0133 0.0003
Eisosomes 0.0008 0 0.0001 0.0002 0.0002 0 0.0004 0 0 0 0.0004 0.0001 0.0003 0 0 0.0001 0.0003 0
Endoplasmic Reticulum 0.0077 0.0026 0.0006 0.0023 0.0015 0.0004 0.0023 0.0015 0.0008 0.0005 0.004 0.0013 0.0048 0.0016 0.0005 0.0031 0.0006 0.0006
Endosome 0.0301 0.0028 0.0032 0.0316 0.0242 0.0015 0.0223 0.0008 0.0129 0.0083 0.0797 0.0082 0.0116 0.0007 0.0066 0.0084 0.0124 0.0005
Golgi 0.013 0.0007 0.0017 0.0302 0.0094 0.001 0.0107 0 0.0039 0.0014 0.0274 0.0038 0.0049 0 0.0066 0.0014 0.0087 0
Lipid Particles 0.0263 0.0008 0.0012 0.0232 0.0503 0.0024 0.0246 0.0001 0.0027 0.0022 0.0157 0.0096 0.0069 0.0001 0.0033 0.0011 0.0037 0
Mitochondria 0.0086 0.005 0.0013 0.0186 0.006 0.0011 0.0127 0.0003 0.0036 0.0009 0.0354 0.0137 0.004 0.0003 0.0022 0.0004 0.0028 0.0002
None 0.0201 0.0222 0.0155 0.0093 0.0062 0.0104 0.0448 0.0927 0.0256 0.0009 0.0771 0.0276 0.0998 0.0382 0.0589 0.0583 0.1221 0.0225
Nuclear Periphery 0.0628 0.021 0.021 0.0131 0.0147 0.0075 0.0199 0.0099 0.0083 0.0121 0.0141 0.0146 0.0151 0.009 0.0256 0.0187 0.0104 0.0076
Nucleolus 0.0168 0.0166 0.0141 0.0086 0.0152 0.0123 0.0059 0.0106 0.0327 0.0186 0.0046 0.0109 0.0063 0.019 0.0243 0.0278 0.0166 0.0115
Nucleus 0.5382 0.8598 0.8797 0.7244 0.7268 0.9224 0.6465 0.7895 0.8511 0.8841 0.5842 0.8462 0.7305 0.878 0.8261 0.8058 0.7252 0.8904
Peroxisomes 0.0179 0.0008 0.0011 0.0333 0.0137 0.0009 0.0306 0 0.0033 0.0038 0.0285 0.0043 0.0028 0 0.0031 0.0002 0.0052 0
Punctate Nuclear 0.0678 0.0326 0.0226 0.0136 0.0708 0.021 0.0521 0.0237 0.0151 0.05 0.0365 0.029 0.0214 0.0131 0.0235 0.0439 0.0223 0.0368
Vacuole 0.0078 0.0022 0.003 0.0161 0.0013 0.0003 0.0038 0.0007 0.006 0.0003 0.0066 0.0008 0.002 0.0009 0.0007 0.0003 0.0008 0.0003
Vacuole Periphery 0.0035 0.0004 0.0004 0.0021 0.0015 0.0003 0.0018 0.0001 0.0012 0.0003 0.0048 0.0008 0.0008 0.0001 0.0004 0.0004 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.1118 7.5135 7.1365 11.6589 13.2948 15.4566 10.4838 7.9988 10.01 14.7461
Translational Efficiency 0.6218 0.4794 0.5042 0.4797 0.3286 0.421 0.3438 0.3372 0.4155 0.3935

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
254 126 155 859 1370 1329 1573 86 1624 1455 1728 945

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 682.67 802.39 1064.49 876.14 663.00 753.72 884.00 899.22 666.08 757.93 900.19 878.24
Standard Deviation 73.57 108.86 150.56 115.02 86.48 110.07 104.00 113.96 84.89 110.82 120.58 115.11
Intensity Change Log 2 — 0.233115 0.640902 0.359973 — 0.185020 0.415037 0.439665 — 0.209619 0.534032 0.399787

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000058 0.002406 0.000730 0.000997 0.000104 0.000333 0.000427 0.000536 0.000097 0.000512 0.000454 0.000955
Bud Neck 0.009462 0.012900 0.001686 0.017068 0.004455 0.012018 0.007645 0.004907 0.005238 0.012095 0.007111 0.015961
Bud Site 0.001422 0.008505 0.001755 0.024670 0.001275 0.007456 0.003219 0.010324 0.001298 0.007547 0.003088 0.023365
Cell Periphery 0.000111 0.000219 0.000194 0.000162 0.000118 0.000205 0.000117 0.000207 0.000117 0.000206 0.000124 0.000166
Cytoplasm 0.206590* 0.102243* 0.119838* 0.080262 0.213165* 0.191409* 0.039500 0.139667* 0.212137* 0.183687* 0.046707 0.085668
Cytoplasmic Foci 0.080046 0.031986 0.010507 0.018856 0.028879 0.059224 0.000992 0.016504 0.036882 0.056866 0.001845 0.018642
Eisosomes 0.000085 0.000096 0.000090 0.000040 0.000040 0.000073 0.000027 0.000052 0.000047 0.000075 0.000032 0.000041
Endoplasmic Reticulum 0.001235 0.005102 0.011310 0.007569 0.001550 0.003046 0.006417 0.007351 0.001500 0.003224 0.006856 0.007549
Endosome 0.003628 0.003102 0.002674 0.028195 0.002025 0.006129 0.000769 0.034249 0.002275 0.005867 0.000940 0.028746
Golgi 0.000387 0.001158 0.000097 0.016319 0.000160 0.001714 0.000051 0.020377 0.000196 0.001666 0.000055 0.016688
Lipid Particles 0.009500 0.004832 0.002306 0.001180 0.003798 0.008717 0.000612 0.001512 0.004690 0.008381 0.000764 0.001210
Mitochondria 0.003742 0.005338 0.000460 0.016587 0.002408 0.011086 0.001229 0.062865 0.002617 0.010588 0.001160 0.020798
Mitotic Spindle 0.001591 0.013191 0.000151 0.113766* 0.001295 0.010631 0.005147 0.036591 0.001342 0.010852 0.004698 0.106743*
None 0.007612 0.011816 0.003512 0.006798 0.019156 0.008965 0.007879 0.006898 0.017351 0.009212 0.007487 0.006807
Nuclear Periphery 0.002221 0.002634 0.001471 0.006750 0.002062 0.002685 0.001969 0.003232 0.002087 0.002680 0.001924 0.006430
Nuclear Periphery Foci 0.001296 0.003373 0.002420 0.003598 0.002958 0.002225 0.000542 0.014482 0.002698 0.002325 0.000710 0.004588
Nucleolus 0.007619 0.008169 0.001862 0.002364 0.012271 0.009808 0.002223 0.002010 0.011543 0.009666 0.002191 0.002332
Nucleus 0.624596* 0.739177* 0.768333* 0.573184* 0.697634* 0.631226* 0.909527* 0.522521* 0.686211* 0.640574* 0.896862* 0.568573*
Peroxisomes 0.004711 0.014778 0.000366 0.001869 0.001497 0.008765 0.000204 0.000656 0.002000 0.009286 0.000219 0.001758
Vacuole 0.033732 0.027797 0.070057 0.058419 0.004834 0.022594 0.011210 0.093682 0.009354 0.023044 0.016488 0.061628
Vacuole Periphery 0.000355 0.001177 0.000183 0.021347 0.000316 0.001692 0.000294 0.021376 0.000322 0.001647 0.000284 0.021350

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.13 -7.34 -13.95 0.96 -1.00 -4.34 -12.67 -4.46 -3.61 -2.55 -3.89 -14.57 -14.03 -5.90 -6.64
Bud Neck -1.21 2.36 -2.59 -1.29 -10.94 -5.36 -4.12 -2.59 1.32 0.25 -5.15 -3.16 -7.74 -2.45 -4.62
Bud Site -3.08 -2.38 -5.71 -0.53 -4.97 -4.26 -3.34 -2.76 -0.36 -1.86 -4.80 -3.62 -6.12 -1.95 -4.69
Cell Periphery -2.93 -4.61 -1.68 2.40 3.95 -4.29 0.96 -1.27 2.06 -1.76 -4.82 0.02 -1.49 4.06 -1.30
Cytoplasm 5.18 4.78 9.03 2.19 2.33 1.74 23.30 7.74 6.81 -2.89 2.80 24.16 18.40 15.45 -5.56
Cytoplasmic Foci 4.05 8.13 8.75 4.57 1.51 -7.35 15.04 6.53 11.86 -2.28 -5.05 16.99 14.75 17.89 -3.75
Eisosomes -1.60 -1.54 1.39 2.41 3.06 -6.42 4.03 -4.36 -2.40 -5.30 -5.93 3.90 -5.41 0.74 -9.39
Endoplasmic Reticulum -4.90 -8.93 -18.87 -8.09 -0.74 -5.85 -13.99 -6.72 -5.83 -2.83 -7.28 -15.96 -20.29 -16.83 -7.46
Endosome -0.28 0.42 -2.28 -2.01 -2.64 -5.20 3.37 -1.77 -0.62 -2.20 -5.16 3.78 -3.59 -0.47 -5.03
Golgi -1.42 1.79 -3.23 -2.04 -3.57 -3.90 7.40 -1.15 -0.71 -1.20 -4.01 6.41 -3.59 -1.73 -3.83
Lipid Particles 1.78 4.05 4.61 2.98 3.03 -4.43 8.14 4.14 7.25 -3.65 -3.55 9.22 7.79 8.84 -5.62
Mitochondria -0.35 1.89 -0.94 -0.43 -5.10 -4.88 1.82 -1.29 -0.28 -1.43 -4.82 2.38 -2.73 2.14 -4.19
Mitotic Spindle -1.54 1.08 -8.68 -3.23 -9.45 -4.60 -3.57 -2.24 -1.43 -1.70 -4.77 -3.38 -9.46 -7.18 -8.16
None -0.71 2.30 0.74 1.52 -4.34 4.75 6.09 5.90 2.26 0.73 4.41 6.22 6.27 2.58 -0.27
Nuclear Periphery -0.60 0.99 -9.08 -7.67 -10.68 -2.31 -3.83 -5.07 -3.58 -3.27 -2.38 -3.67 -10.45 -9.12 -9.15
Nuclear Periphery Foci -0.97 -1.35 -1.02 0.63 0.77 2.02 4.26 -0.06 -0.95 -1.72 1.59 4.05 1.91 0.07 -3.88
Nucleolus -0.30 2.77 2.17 2.64 -2.86 4.78 8.65 5.36 2.23 -0.46 4.43 8.93 8.98 6.29 0.12
Nucleus -2.78 -3.14 -2.45 1.31 1.65 4.94 -21.93 0.86 -0.84 6.88 3.61 -22.17 0.35 -2.79 18.34
Peroxisomes -1.90 3.29 3.14 2.79 -1.70 -6.11 8.28 4.05 7.43 -1.57 -6.16 8.27 6.84 8.27 -3.37
Vacuole -0.07 -4.87 -7.07 -5.82 1.17 -11.33 -9.18 -5.74 -4.88 -5.03 -7.08 -7.76 -16.92 -14.10 -12.92
Vacuole Periphery -1.43 1.95 -2.91 -2.21 -3.02 -3.57 0.68 -1.45 -0.96 -1.48 -3.72 0.87 -3.17 -2.04 -3.24
Standard name
Human Ortholog
Description Subunit of the SMC5-SMC6 complex; the SMC5-SMC6 complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; supports nucleolar function; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
Localization
Cell Percentages nucleus (59%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Smc5

Smc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available