Standard name
Human Ortholog
Description DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.75 0.95 0.97 0.92 0.93 0.84 0.69 0.73 0.57 0.58 0.46 0.55 0.99 1.0 1.0 0.93 0.94 – 0.6 0.81 0.61 0.87 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.47 0.15 0 0.11 0.11 0.28 0.45 0.38 0.6 0.58 0.76 0.66 0 0 0 0 0 – 0.29 0.12 0.23 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 – 1 0 6 1 2 3
Bud 0 0 1 2 2 10 20 14 25 20 32 22 0 0 0 1 0 – 0 0 3 2 8 8
Bud Neck 0 0 1 0 1 3 7 3 1 7 2 0 2 4 2 0 1 – 0 1 2 0 0 3
Bud Site 0 0 0 0 0 0 4 3 1 6 6 4 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 3 1 2 1 0 2 1 2 3 4 3 1 5 2 – 1 0 1 2 4 4
Cytoplasm 51 74 242 205 364 429 508 451 251 331 188 287 433 537 531 149 149 – 51 77 161 265 456 458
Endoplasmic Reticulum 2 1 1 2 0 0 0 2 0 0 0 0 0 1 1 13 11 – 0 0 1 0 0 3
Endosome 0 0 0 0 9 2 3 1 2 1 1 1 1 1 0 2 2 – 2 0 7 0 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 – 0 0 3 1 2 0
Mitochondria 32 12 9 24 43 144 333 237 263 332 311 347 1 0 1 6 5 – 24 11 61 13 13 12
Nucleus 0 0 0 0 1 0 2 5 0 3 3 3 0 2 1 3 3 – 0 0 0 0 2 1
Nuclear Periphery 0 0 0 0 0 1 1 0 0 1 1 1 0 0 0 0 0 – 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 2 1 0 2 2 0 0 0 0 0 – 0 0 0 0 1 0
Peroxisomes 0 0 0 0 2 0 0 0 0 0 2 1 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 2 4 3 11 23 11 12 13 8 15 1 0 0 1 0 – 0 0 4 2 11 12
Vac/Vac Membrane 0 0 1 0 2 8 10 8 2 6 8 10 1 0 0 2 6 – 0 1 4 2 5 7
Unique Cell Count 68 78 250 223 393 513 741 616 440 573 407 524 438 538 532 160 159 85 95 266 305 523 537
Labelled Cell Count 85 87 257 240 428 610 912 737 560 722 566 696 444 548 537 184 181 85 95 266 305 523 537


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.5 3.7 3.4 3.6 2.9 2.8 2.9 2.7 2.6 2.4 2.6 4.6 4.7 4.7 5.6 5.2 4.5 4.8 5.1 5.1
Std Deviation (1e-4) 0.4 0.5 1.6 1.4 1.7 0.8 0.7 1.2 0.7 0.9 0.7 0.5 1.1 1.0 1.3 1.9 1.3 1.0 1.2 1.5 1.4
Intensity Change (Log2) – – – -0.1 -0.02 -0.32 -0.41 -0.32 -0.43 -0.49 -0.6 -0.53 0.33 0.36 0.34 0.59 0.49 0.3 0.39 0.46 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4956 0.3384 0.4036 0.0652 0.5612 0.5014 -2.3007 -2.1182 -1.8649 -1.777 -1.5146 -1.9723 -2.4878 -2.4734 -2.6164 -2.2286 -2.7427 -2.4793
Actin 0.0516 0.0073 0.0021 0.0033 0.0612 0.004 0.0192 0.0002 0.004 0.0004 0.1051 0.0064 0.0487 0.0024 0.0132 0.0005 0.0053 0.016
Bud 0.0085 0.0075 0.0038 0.0113 0.0027 0.0019 0.0011 0.0038 0.0004 0.0004 0.0057 0.0003 0.0007 0.0011 0.0003 0.0001 0.0001 0.0003
Bud Neck 0.0044 0.0001 0.0008 0.0013 0.0036 0.0014 0.003 0.0001 0.0005 0.0016 0.0009 0.0004 0.0038 0.0008 0.0007 0.0002 0 0.0034
Bud Periphery 0.0084 0.0071 0.0013 0.0219 0.006 0.0048 0.002 0.0043 0.0007 0.0004 0.0077 0.0006 0.0009 0.0025 0.0004 0.0001 0.0003 0.0008
Bud Site 0.0114 0.0038 0.0097 0.0155 0.0083 0.0006 0.0087 0.0024 0.0017 0.031 0.0171 0.0017 0.0114 0.0083 0.0028 0.0003 0.0003 0.0011
Cell Periphery 0.0012 0.0002 0.0002 0.0035 0.0006 0.0002 0.0005 0.0003 0.0001 0.0003 0.0003 0.0001 0.0003 0.0003 0.0001 0.0001 0 0.0001
Cytoplasm 0.0282 0.02 0.0122 0.0187 0.0673 0.0235 0.0308 0.11 0.079 0.0832 0.0226 0.087 0.0191 0.0445 0.0499 0.051 0.0548 0.0402
Cytoplasmic Foci 0.0281 0.005 0.0215 0.0284 0.0632 0.0122 0.0358 0.023 0.0352 0.0277 0.0177 0.008 0.0304 0.0039 0.0197 0.0093 0.0042 0.012
Eisosomes 0.0006 0.0002 0.0002 0.0007 0.001 0.0002 0.0003 0.0001 0.0002 0.0004 0.0009 0.0001 0.0006 0.0005 0.0004 0.0004 0.0002 0.0001
Endoplasmic Reticulum 0.0045 0.0003 0.0004 0.001 0.0032 0.0007 0.0058 0.0009 0.0015 0.0007 0.001 0.0006 0.0014 0.0005 0.001 0.0004 0.0009 0.0002
Endosome 0.0228 0.0002 0.006 0.0068 0.016 0.0078 0.0134 0.0014 0.0222 0.0123 0.0093 0.0045 0.0165 0.0002 0.0062 0.0038 0.0119 0.0003
Golgi 0.0099 0.0002 0.0064 0.0049 0.0071 0.0051 0.0044 0.0002 0.0126 0.0005 0.027 0.0037 0.0109 0.0001 0.0043 0.0026 0.004 0.0003
Lipid Particles 0.0299 0.0002 0.0098 0.0026 0.0092 0.0026 0.0184 0.0009 0.0163 0.001 0.0263 0.0055 0.0257 0.0003 0.0079 0.0339 0.008 0.0005
Mitochondria 0.0154 0.0012 0.0055 0.0068 0.0336 0.0039 0.0028 0.0005 0.0075 0.0005 0.0385 0.0096 0.0085 0.0003 0.0046 0.0006 0.0083 0.0005
None 0.6939 0.94 0.89 0.8354 0.6862 0.9103 0.809 0.8481 0.7992 0.8106 0.6919 0.8671 0.78 0.9327 0.8688 0.89 0.8909 0.9213
Nuclear Periphery 0.0139 0.0002 0.001 0.0006 0.0046 0.005 0.005 0.0003 0.0014 0.0006 0.0009 0.0005 0.0037 0.0002 0.0006 0.0003 0.0047 0.0001
Nucleolus 0.0028 0.0013 0.0037 0.0034 0.0025 0.0006 0.001 0.0003 0.001 0.0063 0.0003 0.0001 0.0013 0.0001 0.0002 0.0008 0.0003 0.0001
Nucleus 0.0329 0.0018 0.0052 0.0106 0.0023 0.0022 0.0019 0.0006 0.0007 0.0133 0.0004 0.0003 0.0017 0.0002 0.0003 0.0003 0.0006 0.0001
Peroxisomes 0.0108 0.0018 0.0046 0.0116 0.0037 0.0056 0.0208 0.0008 0.0116 0.0003 0.0224 0.002 0.0174 0.0003 0.0164 0.003 0.002 0.0019
Punctate Nuclear 0.0138 0.0012 0.0146 0.0093 0.0128 0.0059 0.0086 0.0011 0.0019 0.0048 0.0023 0.0005 0.0151 0.0003 0.0009 0.0022 0.0016 0.0006
Vacuole 0.0041 0.0003 0.0008 0.0019 0.003 0.001 0.0057 0.0008 0.0015 0.0036 0.0008 0.0007 0.0011 0.0003 0.001 0.0004 0.0005 0.0001
Vacuole Periphery 0.0032 0.0001 0.0002 0.0004 0.0017 0.0006 0.0018 0.0001 0.0008 0.0002 0.0008 0.0003 0.001 0 0.0003 0.0001 0.001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.8614 3.4519 4.4825 5.0303 1.8774 5.7889 3.9265 6.478 5.2259 5.4403
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
57 324 1981 1323 1644 163 142 486 1701 487 2123 1809

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.55 748.12 729.74 739.19 634.54 723.22 853.86 779.91 633.74 739.79 738.04 750.13
Standard Deviation 88.54 84.50 80.63 91.40 76.35 204.29 82.27 96.54 76.91 137.32 86.49 94.55
Intensity Change Log 2 — 0.293160 0.257273 0.275836 — 0.188723 0.428288 0.297597 — 0.240881 0.346958 0.286967

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000177 0.000682 0.000145 0.000637 0.000084 0.000627 0.000108 0.000412 0.000087 0.000663 0.000143 0.000577
Bud Neck 0.016531 0.059765 0.010260 0.008289 0.010683 0.017985 0.000784 0.004804 0.010879 0.045781 0.009626 0.007353
Bud Site 0.002504 0.029130 0.014671 0.015965 0.003107 0.011970 0.000752 0.008603 0.003087 0.023387 0.013740 0.013987
Cell Periphery 0.000208 0.000196 0.000163 0.000218 0.000154 0.000526 0.000278 0.000141 0.000156 0.000307 0.000171 0.000197
Cytoplasm 0.412134* 0.293027* 0.481245* 0.415271* 0.530613* 0.325158* 0.271608* 0.450373* 0.526642* 0.303781* 0.467223* 0.424701*
Cytoplasmic Foci 0.318402* 0.324523* 0.019156 0.053328 0.190971* 0.290187* 0.056805 0.033267 0.195241* 0.313031* 0.021674 0.047938
Eisosomes 0.000252 0.000192 0.000034 0.000097 0.000167 0.000203 0.000148 0.000064 0.000170 0.000196 0.000042 0.000088
Endoplasmic Reticulum 0.001127 0.000857 0.000944 0.003449 0.000601 0.000616 0.001876 0.001546 0.000618 0.000776 0.001006 0.002938
Endosome 0.035607 0.035985 0.002886 0.006681 0.006111 0.022090 0.005451 0.004057 0.007099 0.031335 0.003057 0.005976
Golgi 0.002373 0.006917 0.000367 0.000596 0.001510 0.003869 0.000091 0.001483 0.001539 0.005897 0.000349 0.000834
Lipid Particles 0.023130 0.006785 0.001130 0.003052 0.007578 0.011237 0.005453 0.001928 0.008099 0.008275 0.001419 0.002750
Mitochondria 0.010351 0.018989 0.001119 0.002355 0.003369 0.018795 0.000214 0.004725 0.003603 0.018924 0.001058 0.002992
Mitotic Spindle 0.001103 0.006176 0.005809 0.004819 0.000348 0.027834 0.000039 0.017832 0.000373 0.013425 0.005423 0.008315
None 0.003145 0.001271 0.002865 0.002016 0.004574 0.002739 0.003851 0.017395 0.004526 0.001762 0.002931 0.006148
Nuclear Periphery 0.000920 0.000381 0.002152 0.001833 0.000425 0.000411 0.001439 0.001432 0.000441 0.000391 0.002104 0.001725
Nuclear Periphery Foci 0.000931 0.000659 0.000508 0.001121 0.000230 0.000447 0.001189 0.000865 0.000253 0.000588 0.000554 0.001052
Nucleolus 0.004529 0.003057 0.000703 0.000404 0.000803 0.001387 0.000956 0.000552 0.000928 0.002498 0.000720 0.000444
Nucleus 0.115021 0.094120 0.408861* 0.326300* 0.206024* 0.164383* 0.565461* 0.324469* 0.202974* 0.117637 0.419336* 0.325808*
Peroxisomes 0.017384 0.035103 0.000492 0.002609 0.004118 0.021063 0.000592 0.000801 0.004562 0.030404 0.000498 0.002124
Vacuole 0.029106 0.079019 0.045664 0.150197 0.027959 0.077340 0.082708 0.124955 0.027997 0.078457 0.048142 0.143416
Vacuole Periphery 0.005065 0.003167 0.000826 0.000762 0.000575 0.001132 0.000196 0.000295 0.000725 0.002486 0.000784 0.000637

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.55 0.34 -3.22 0.58 -4.34 -2.73 -2.82 -3.33 1.46 -1.90 -4.19 -2.37 -5.91 1.12 -4.71
Bud Neck -4.95 1.16 1.66 7.28 2.18 -1.50 10.50 5.24 2.85 -3.24 -6.80 0.98 4.02 7.64 2.76
Bud Site -6.09 -5.50 -4.69 3.78 0.61 -2.91 2.66 -2.61 1.79 -4.09 -6.58 -5.36 -4.99 4.01 0.99
Cell Periphery 0.28 1.24 0.75 0.74 -0.98 -3.10 -4.95 4.00 3.40 6.40 -3.43 -0.12 -0.48 3.33 -0.06
Cytoplasm 2.84 -1.06 1.55 -3.53 10.50 9.67 13.53 14.55 -0.46 -3.81 16.89 10.08 20.77 -3.18 10.40
Cytoplasmic Foci -0.18 10.22 9.37 21.07 -9.87 -5.52 16.38 30.59 15.45 3.33 -10.10 35.01 30.49 25.89 -8.65
Eisosomes 1.39 5.42 3.88 5.81 -13.15 -1.52 0.19 8.81 5.70 5.68 -1.66 14.06 8.33 7.87 -12.40
Endoplasmic Reticulum 0.55 -0.55 -6.71 -10.60 -12.56 -0.09 -6.12 -10.31 -7.98 0.28 -0.90 -7.02 -18.99 -13.03 -12.58
Endosome -0.04 2.83 2.65 7.90 -2.53 -3.07 -0.13 4.14 3.55 2.96 -7.62 5.98 3.34 8.48 -2.35
Golgi -3.06 2.94 2.92 5.05 -0.24 -2.19 7.91 2.00 2.90 -1.05 -4.56 5.17 4.94 5.81 -0.54
Lipid Particles 3.04 4.17 3.83 4.40 -7.94 -1.31 2.05 9.69 3.05 5.05 -0.29 12.01 9.54 4.74 -6.67
Mitochondria -1.06 1.60 1.51 3.66 -1.12 -1.88 4.46 1.08 2.05 -1.52 -3.61 3.61 2.34 4.12 -1.45
Mitotic Spindle -2.32 -2.84 -2.63 0.56 0.33 -2.33 -0.74 -3.65 0.90 -3.36 -3.13 -4.48 -5.40 1.22 -1.62
None 1.62 0.35 1.11 -1.94 2.99 1.60 1.12 -1.25 -1.85 -1.65 3.60 2.42 0.99 -2.17 -0.99
Nuclear Periphery 1.83 -5.38 -5.53 -13.36 0.08 -0.11 -6.49 -12.97 -12.96 -2.00 0.52 -13.68 -18.47 -17.38 -0.08
Nuclear Periphery Foci 0.69 1.25 -1.53 -2.57 -3.98 -2.98 -5.56 -6.77 -4.82 0.70 -1.88 -1.81 -9.25 -3.82 -4.11
Nucleolus 0.57 1.70 1.82 1.70 2.55 -2.03 -1.23 2.75 3.15 3.59 -1.52 1.30 3.80 2.02 2.78
Nucleus 0.97 -14.90 -10.37 -22.33 9.65 2.44 -13.53 -9.53 -8.04 8.03 9.37 -29.89 -17.27 -21.79 11.84
Peroxisomes -2.23 2.44 2.20 8.04 -4.19 -3.21 5.86 5.90 3.95 0.57 -7.81 6.49 4.31 8.84 -4.05
Vacuole -6.19 -4.51 -22.09 -16.47 -23.36 -4.94 -6.58 -19.24 -11.78 -6.61 -10.01 -12.65 -34.67 -20.11 -26.69
Vacuole Periphery 0.54 1.27 1.35 3.22 0.92 -2.54 5.29 5.13 4.41 0.43 -3.08 0.19 1.97 3.70 1.21
Standard name
Human Ortholog
Description DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (18%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ntg2

Ntg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ntg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available