Standard name
Human Ortholog
Description Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.11 0 0 0 0 0 0 – 0 0 0 0.1 0.06 0 0 0.07 0 0.12 0.14 0.12 0.15 0.14 0.21
Bud 0.08 0 0.07 0.13 0.13 0.12 0.09 0.11 – 0.11 0.12 0.14 0.05 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 – 0 0 0 0.09 0 0.05 0 0.09 0.06 0 0.08 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.45 0.57 0.49 0.28 0.33 0.4 0.45 0.45 – 0.31 0.23 0.19 0.45 0.64 0.72 0.23 0.18 0.2 0.07 0.15 0.1 0.05 0.06 0.05
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0.06 0 0 0 – 0 0 0 0.05 0.06 0.07 0.12 0.11 0.16 0.21 0.17 0.16 0.13 0.11 0.12
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.3 0.16 0.25 0.32 0.45 0.46 0.45 – 0.52 0.69 0.67 0 0 0 0.2 0.1 0.08 0.21 0.23 0.24 0.24 0.28 0.26
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0.06 0.09 0.27 0.17 0 0.05 0 – 0.05 0.06 0.12 0 0.05 0.05 0.12 0.26 0.21 0.2 0.09 0.21 0.24 0.23 0.19
SpindlePole 0.23 0.07 0.22 0.38 0.29 0.16 0.18 0.2 – 0.24 0.17 0.23 0.35 0.21 0.18 0.46 0.44 0.47 0.09 0.07 0.11 0.09 0.07 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 9 4 2 2 9 0 0 – 0 3 1 24 17 5 8 13 7 49 15 35 42 65 107
Bud 24 2 15 35 64 62 59 87 – 59 54 81 13 10 3 16 14 9 4 1 1 1 9 10
Bud Neck 6 1 14 5 20 23 12 16 – 13 3 11 21 12 15 8 15 10 11 8 5 11 15 21
Bud Site 4 2 6 10 10 18 8 11 – 17 14 30 1 0 1 9 5 2 – – – – – –
Cell Periphery 2 1 0 1 0 2 2 0 – 0 0 1 2 0 1 3 1 1 0 0 2 0 6 8
Cytoplasm 130 48 112 76 167 207 283 346 – 161 108 108 106 170 216 55 32 34 29 16 28 14 29 23
Endoplasmic Reticulum 4 4 3 0 1 3 1 1 – 0 4 2 4 10 4 5 7 5 1 0 0 0 2 5
Endosome 20 2 5 10 29 2 6 9 – 7 2 5 11 15 21 29 20 26 85 18 46 36 51 59
Golgi 2 0 0 0 1 0 0 0 – 0 0 0 0 0 0 5 1 6 8 0 0 2 2 3
Mitochondria 61 25 37 69 159 232 290 349 – 271 323 375 2 0 1 47 17 14 83 25 69 70 130 135
Nucleus 0 0 0 0 1 3 1 2 – 3 2 1 0 0 1 1 2 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 2 0 1 0 – 1 0 2 0 0 0 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 6 1 0 3 – 2 2 5 1 0 0 3 0 2 0 0 0 0 0 0
Peroxisomes 24 5 21 73 87 23 33 34 – 28 27 65 10 14 15 28 45 35 80 10 61 69 108 96
SpindlePole 67 6 50 103 143 84 112 150 – 125 80 131 83 55 54 110 76 79 34 7 33 24 32 24
Vac/Vac Membrane 4 2 0 2 2 1 3 3 – 3 2 0 3 0 1 2 2 3 5 3 2 5 11 12
Unique Cell Count 288 84 227 273 499 520 624 769 518 465 562 237 267 301 239 174 167 400 112 294 287 469 516
Labelled Cell Count 354 107 267 386 694 670 811 1011 690 624 818 281 303 338 330 250 235 400 112 294 287 469 516


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.0 3.5 3.6 3.6 3.3 3.0 3.0 – 2.7 2.7 2.8 5.2 4.9 5.1 5.8 5.5 5.8 5.5 5.5 5.5
Std Deviation (1e-4) 0.8 0.4 0.9 1.6 1.8 1.3 1.6 1.1 – 1.2 0.9 0.9 0.8 1.0 1.6 1.7 1.6 1.8 1.6 1.3 1.3
Intensity Change (Log2) – – – 0.05 0.06 -0.08 -0.23 -0.24 – -0.35 -0.37 -0.32 0.57 0.49 0.54 0.74 0.66 0.74 0.66 0.65 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6633 -0.1356 -0.3744 -0.5741 -0.9564 -0.4016 1.5269 1.8886 1.7399 1.7069 1.9154 1.9699 -0.4937 0.0741 -0.4706 -0.103 -0.4846 -0.3647
Actin 0.0075 0.0269 0.0276 0.0083 0.0091 0.0161 0.0369 0.0422 0.0517 0.0415 0.0077 0.0194 0.0209 0.0238 0.0375 0.0058 0.0081 0.0116
Bud 0.0003 0.0004 0.0019 0.0159 0.0001 0.0003 0.0011 0.0023 0.0008 0.0005 0.0002 0.0002 0.0004 0.0015 0.0034 0.0031 0.0001 0.0007
Bud Neck 0.0014 0.003 0.0244 0.0068 0.0009 0.0129 0.0026 0.0044 0.0204 0.1069 0.0005 0.0056 0.0012 0.0075 0.0206 0.0337 0.002 0.013
Bud Periphery 0.0002 0.0005 0.0024 0.0049 0.0002 0.0004 0.0025 0.0031 0.0027 0.0006 0.0002 0.0005 0.0016 0.0013 0.002 0.0018 0.0003 0.0013
Bud Site 0.0017 0.0231 0.0125 0.0083 0.0003 0.0009 0.0058 0.0531 0.0118 0.0123 0.0005 0.0005 0.0054 0.0424 0.0105 0.004 0.0003 0.0006
Cell Periphery 0.0001 0.0002 0.0004 0.0005 0 0.0001 0.0008 0.0003 0.0007 0.0004 0 0.0002 0.0002 0.0004 0.0002 0.0003 0 0.0002
Cytoplasm 0.0101 0.0119 0.013 0.0053 0.0017 0.0036 0.0119 0.0222 0.0106 0.0065 0.0232 0.0143 0.0094 0.0108 0.0078 0.0135 0.0031 0.0097
Cytoplasmic Foci 0.264 0.2837 0.2304 0.1807 0.3176 0.3114 0.3115 0.2375 0.2557 0.1523 0.182 0.3067 0.2751 0.2153 0.2374 0.3049 0.2145 0.2812
Eisosomes 0.0004 0.0003 0.0009 0.0003 0 0.0001 0.001 0.0004 0.0018 0.0003 0.0002 0.0003 0.0008 0.0004 0.0007 0.0002 0.0002 0.0004
Endoplasmic Reticulum 0.0019 0.0018 0.0006 0.0012 0 0.0001 0.0018 0.0008 0.0004 0.0003 0.0002 0.0002 0.0004 0.0003 0.0003 0.0001 0.0001 0.0002
Endosome 0.0304 0.0395 0.0282 0.0169 0.015 0.0146 0.035 0.0224 0.0119 0.0105 0.0133 0.0142 0.0339 0.0176 0.0234 0.0388 0.0376 0.0121
Golgi 0.0143 0.0242 0.012 0.0079 0.0064 0.0076 0.0134 0.0087 0.0083 0.0127 0.0092 0.008 0.0155 0.0154 0.0127 0.011 0.0113 0.0078
Lipid Particles 0.0899 0.0413 0.0338 0.0513 0.0552 0.0431 0.0427 0.0245 0.0205 0.0386 0.0228 0.0273 0.0411 0.026 0.0143 0.0211 0.043 0.0314
Mitochondria 0.0121 0.006 0.0295 0.0048 0.006 0.0077 0.0111 0.0044 0.0146 0.0144 0.0046 0.0101 0.0126 0.0103 0.0131 0.0042 0.017 0.0111
None 0.0799 0.0938 0.0712 0.0086 0.0049 0.0496 0.1 0.159 0.085 0.0999 0.0706 0.0971 0.085 0.1371 0.086 0.0481 0.0752 0.0659
Nuclear Periphery 0.0106 0.0003 0.0007 0.0025 0.0002 0.0002 0.0013 0.0017 0.0002 0.0001 0.0004 0.0001 0.0003 0.0002 0.0002 0.0001 0.0002 0.0002
Nucleolus 0.0033 0.0014 0.0016 0.0077 0.0004 0.0005 0.0012 0.0032 0.0009 0.0004 0.0006 0.0004 0.0009 0.0045 0.0009 0.0015 0.0004 0.0007
Nucleus 0.0106 0.001 0.0016 0.0099 0.0001 0.0002 0.0012 0.0015 0.0003 0.0002 0.0005 0.0003 0.0006 0.0016 0.0007 0.0012 0.0003 0.0008
Peroxisomes 0.446 0.4251 0.4931 0.6234 0.5707 0.5134 0.4079 0.3983 0.4959 0.4984 0.6368 0.4833 0.4863 0.4672 0.5202 0.4936 0.5778 0.5419
Punctate Nuclear 0.013 0.0139 0.0128 0.033 0.0099 0.017 0.0079 0.0081 0.0044 0.0026 0.0261 0.0107 0.0068 0.015 0.007 0.0114 0.0075 0.0087
Vacuole 0.0014 0.0014 0.0009 0.0016 0.001 0.0003 0.0017 0.0013 0.0012 0.0005 0.0003 0.0006 0.0013 0.0014 0.0008 0.0015 0.0007 0.0004
Vacuole Periphery 0.0008 0.0003 0.0004 0.0002 0.0002 0.0001 0.0005 0.0004 0.0002 0.0001 0.0001 0.0002 0.0003 0.0002 0.0002 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.3945 12.8434 6.989 6.669 6.2093 5.7437 6.493 8.7647 6.7101 8.6568
Translational Efficiency 3.8051 0.6635 0.9949 1.0053 1.2656 1.7076 1.8359 1.0678 1.3495 1.2201

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1755 1510 146 1348 1848 1979 1860 1248 3603 3489 2006 2596

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 635.27 718.53 882.71 819.97 645.06 684.82 802.03 832.62 640.29 699.41 807.90 826.05
Standard Deviation 71.85 92.80 84.06 115.08 88.58 82.26 92.88 108.04 81.01 88.57 94.62 111.93
Intensity Change Log 2 — 0.177679 0.474570 0.368201 — 0.086291 0.314223 0.368225 — 0.132359 0.396010 0.368213

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007824 0.011103 0.009707 0.060447 0.003589 0.014625 0.021159 0.071558 0.005652 0.013101 0.020326 0.065788
Bud Neck 0.024632 0.025139 0.020977 0.015300 0.029290 0.030049 0.028936 0.013416 0.027021 0.027924 0.028356 0.014394
Bud Site 0.006053 0.014707 0.037333 0.118763* 0.006292 0.016345 0.040749 0.071914 0.006175 0.015636 0.040500 0.096241
Cell Periphery 0.000323 0.000282 0.000503 0.000892 0.000231 0.000332 0.000186 0.000754 0.000275 0.000310 0.000209 0.000826
Cytoplasm 0.117314* 0.114723* 0.172305* 0.162542* 0.107705* 0.070705 0.075722 0.150522* 0.112386* 0.089755 0.082751 0.156763*
Cytoplasmic Foci 0.403460* 0.363956* 0.179786 0.140499 0.392679* 0.276187* 0.179588 0.169378 0.397930* 0.314173* 0.179602 0.154383
Eisosomes 0.002325 0.000850 0.000326 0.000606 0.001160 0.001118 0.000191 0.001223 0.001727 0.001002 0.000201 0.000902
Endoplasmic Reticulum 0.000362 0.000413 0.006619 0.001670 0.000390 0.000216 0.000520 0.000941 0.000376 0.000301 0.000964 0.001320
Endosome 0.019053 0.035228 0.029548 0.025268 0.040249 0.055841 0.030469 0.014236 0.029925 0.046920 0.030402 0.019965
Golgi 0.022189 0.052318 0.001881 0.006604 0.039438 0.086583 0.005036 0.004977 0.031036 0.071753 0.004806 0.005822
Lipid Particles 0.039550 0.023724 0.020192 0.008422 0.046895 0.019350 0.012410 0.008958 0.043318 0.021243 0.012977 0.008680
Mitochondria 0.010627 0.030077 0.000590 0.007106 0.022870 0.062967 0.003600 0.004209 0.016906 0.048733 0.003381 0.005713
Mitotic Spindle 0.000475 0.004383 0.007408 0.041038 0.002870 0.005643 0.031177 0.014746 0.001703 0.005098 0.029447 0.028398
None 0.001718 0.001153 0.002084 0.001674 0.001775 0.000553 0.000971 0.001002 0.001747 0.000813 0.001052 0.001351
Nuclear Periphery 0.000112 0.000126 0.001479 0.000475 0.000225 0.000044 0.000185 0.000104 0.000170 0.000079 0.000279 0.000296
Nuclear Periphery Foci 0.000139 0.000329 0.007095 0.001253 0.000426 0.000097 0.000843 0.000532 0.000286 0.000197 0.001298 0.000906
Nucleolus 0.000858 0.000805 0.004254 0.000379 0.001259 0.000461 0.001905 0.000213 0.001064 0.000610 0.002076 0.000299
Nucleus 0.031807 0.024399 0.257234* 0.027522 0.026398 0.008398 0.022582 0.030376 0.029033 0.015323 0.039660 0.028894
Peroxisomes 0.303955* 0.280531* 0.128766* 0.265176* 0.263560* 0.337369* 0.517255* 0.368429* 0.283237* 0.312770* 0.488980* 0.314814*
Vacuole 0.006890 0.014645 0.111516 0.109864 0.011986 0.011936 0.026298 0.071929 0.009504 0.013108 0.032500 0.091627
Vacuole Periphery 0.000334 0.001110 0.000395 0.004499 0.000713 0.001180 0.000218 0.000583 0.000528 0.001150 0.000231 0.002617

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.24 -0.48 -11.43 -10.32 -9.91 -8.74 -10.14 -15.83 -12.30 -10.94 -7.65 -8.60 -19.33 -15.84 -12.83
Bud Neck -0.20 1.03 4.49 4.57 0.66 -0.35 -0.63 7.53 7.80 8.10 -0.52 -1.32 8.45 8.80 7.97
Bud Site -5.29 -4.49 -17.68 -15.73 -7.76 -7.61 -14.49 -14.38 -11.63 -5.45 -9.12 -15.23 -22.67 -19.46 -10.80
Cell Periphery 0.96 -3.62 -1.66 -1.83 -0.84 -1.41 1.24 -6.92 -3.97 -7.73 -0.76 2.38 -3.34 -2.97 -3.77
Cytoplasm 0.33 -2.21 -8.08 -8.11 -1.62 6.89 2.53 -7.55 -13.12 -9.55 5.40 3.06 -11.12 -15.56 -12.58
Cytoplasmic Foci 4.80 15.63 35.87 31.48 1.15 16.57 30.44 30.98 15.74 2.70 15.48 37.37 47.49 32.51 5.88
Eisosomes 4.96 6.79 6.16 3.94 -3.52 0.21 8.31 0.18 -0.01 -7.47 4.33 9.88 5.51 2.09 -9.39
Endoplasmic Reticulum -0.54 -7.34 -10.04 -10.63 6.11 1.69 -3.85 -6.81 -9.09 -3.59 0.96 -7.79 -12.12 -13.99 -0.86
Endosome -6.74 -1.55 -1.14 5.09 1.09 -4.95 3.30 11.21 15.35 7.48 -8.31 -0.38 7.40 14.73 6.05
Golgi -10.09 14.88 11.19 17.18 -3.59 -12.86 17.94 17.57 25.91 0.48 -16.65 21.94 20.57 30.71 -0.37
Lipid Particles 5.87 4.37 13.45 8.96 3.88 10.20 12.14 13.82 5.67 2.66 11.61 14.21 19.31 10.26 4.13
Mitochondria -7.92 9.96 3.96 10.20 -4.83 -11.07 10.73 9.64 17.85 0.17 -13.80 12.51 10.18 20.35 -1.26
Mitotic Spindle -3.95 -3.03 -10.72 -9.32 -2.55 -2.25 -12.18 -4.49 -3.10 7.10 -4.22 -13.16 -11.40 -9.48 2.97
None 1.20 -0.73 0.52 -0.21 1.07 3.00 0.76 1.35 -0.56 0.80 2.97 0.71 1.22 -0.60 0.64
Nuclear Periphery -0.48 -6.88 -6.09 -5.50 4.49 1.93 -1.62 0.55 -8.38 5.00 1.71 -5.04 -3.81 -6.94 1.12
Nuclear Periphery Foci -1.53 -5.26 -8.65 -5.77 4.65 2.16 -3.99 -1.79 -9.91 3.39 0.86 -6.87 -7.22 -8.95 4.03
Nucleolus 0.50 -2.86 1.56 1.51 3.11 3.48 -3.15 4.52 2.08 5.22 2.56 -4.06 3.94 2.09 5.66
Nucleus 2.75 -10.35 -1.79 -4.23 10.10 9.26 -2.35 -3.88 -11.63 -1.41 8.56 -6.57 -3.99 -11.44 3.82
Peroxisomes 2.25 11.18 8.72 6.50 -6.03 -7.92 -18.56 -4.97 1.58 10.97 -4.34 -15.89 2.50 6.28 16.67
Vacuole -6.54 -9.36 -26.06 -24.64 -2.33 0.00 -12.21 -18.17 -18.06 -11.92 -4.16 -15.75 -31.35 -30.29 -19.45
Vacuole Periphery -4.23 -1.65 -3.24 -2.27 -3.12 -4.65 7.59 0.98 2.98 -1.25 -6.40 6.75 -2.88 -1.48 -3.53
Standard name
Human Ortholog
Description Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex15

Pex15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available