Standard name | |
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Human Ortholog | |
Description | Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 5 | 1 | 1 | 2 | 0 | – | 1 | 0 | 1 | 0 | 0 | 5 | 10 | 1 | 0 | 1 | 1 | 7 | 15 | 1 | 5 | 15 | 16 |
Bud | 13 | 5 | 4 | 15 | 11 | 9 | – | 10 | 6 | 8 | 5 | 10 | 2 | 4 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 3 |
Bud Neck | 1 | 0 | 2 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 2 |
Bud Site | 3 | 0 | 0 | 3 | 2 | 1 | – | 1 | 3 | 6 | 3 | 3 | 0 | 3 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 50 | 17 | 22 | 20 | 14 | 10 | – | 7 | 8 | 15 | 9 | 11 | 13 | 9 | 17 | 5 | 6 | 18 | 18 | 1 | 9 | 13 | 21 | 12 |
Endoplasmic Reticulum | 17 | 20 | 22 | 0 | 4 | 4 | – | 6 | 1 | 3 | 2 | 1 | 0 | 2 | 1 | 0 | 3 | 6 | 3 | 2 | 0 | 2 | 12 | 2 |
Endosome | 47 | 112 | 0 | 20 | 133 | 28 | – | 42 | 1 | 12 | 4 | 2 | 111 | 212 | 269 | 37 | 37 | 58 | 236 | 195 | 75 | 64 | 145 | 173 |
Golgi | 2 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 8 | 1 | 5 | 12 | 27 | 21 | 4 | 3 | 19 | 25 |
Mitochondria | 279 | 123 | 112 | 213 | 199 | 413 | – | 650 | 561 | 670 | 354 | 562 | 92 | 172 | 68 | 7 | 9 | 20 | 80 | 37 | 48 | 38 | 84 | 76 |
Nucleus | 4 | 0 | 2 | 1 | 0 | 2 | – | 7 | 6 | 13 | 6 | 11 | 1 | 1 | 3 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 4 | 7 | 3 | 5 | 11 | 36 | – | 64 | 69 | 71 | 57 | 83 | 1 | 15 | 14 | 4 | 4 | 5 | 5 | 3 | 1 | 1 | 5 | 3 |
Nucleolus | 17 | 6 | 0 | 14 | 28 | 58 | – | 152 | 115 | 169 | 77 | 119 | 6 | 57 | 63 | 19 | 19 | 25 | 3 | 2 | 0 | 0 | 1 | 0 |
Peroxisomes | 13 | 27 | 0 | 55 | 113 | 44 | – | 17 | 4 | 6 | 6 | 3 | 31 | 113 | 107 | 1 | 9 | 9 | 57 | 48 | 27 | 14 | 34 | 54 |
SpindlePole | 60 | 27 | 13 | 46 | 66 | 24 | – | 27 | 10 | 22 | 6 | 8 | 46 | 73 | 115 | 13 | 16 | 17 | 29 | 19 | 11 | 19 | 25 | 26 |
Vac/Vac Membrane | 22 | 34 | 2 | 0 | 4 | 7 | – | 22 | 22 | 37 | 15 | 38 | 5 | 7 | 7 | 7 | 15 | 18 | 14 | 7 | 8 | 5 | 18 | 13 |
Unique Cell Count | 412 | 291 | 153 | 301 | 394 | 483 | 789 | 611 | 791 | 401 | 630 | 229 | 446 | 437 | 58 | 83 | 135 | 495 | 362 | 194 | 175 | 396 | 416 | |
Labelled Cell Count | 541 | 384 | 183 | 393 | 587 | 636 | 1009 | 806 | 1034 | 544 | 853 | 315 | 683 | 675 | 95 | 125 | 193 | 495 | 362 | 194 | 175 | 396 | 416 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.6 | 3.5 | 3.8 | 5.1 | 4.7 | – | 5.5 | 5.3 | 5.6 | 5.4 | 5.2 | 5.4 | 6.5 | 7.3 | 8.9 | 9.3 | 9.1 | 5.0 | 5.4 | 5.6 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.6 | 1.2 | 1.2 | 1.0 | – | 1.2 | 1.5 | 1.6 | 1.5 | 1.4 | 0.9 | 1.2 | 1.7 | 2.3 | 2.5 | 2.2 | 2.0 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.54 | 0.42 | – | 0.64 | 0.6 | 0.69 | 0.63 | 0.59 | 0.62 | 0.89 | 1.06 | 1.34 | 1.41 | 1.39 | 0.52 | 0.63 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.7 | -4.6 | -6.1 | – | 0 | -7.4 | -7.3 | -5.6 | -7.0 | -2.9 | -6.0 | -4.5 | -1.1 | -1.6 | -0.3 |
Endoplasmic Reticulum | 0 | -6.6 | 0 | – | 0 | 0 | 0 | -7.2 | 0 | -5.9 | -7.6 | -7.8 | -3.1 | -2.6 | -2.8 |
Endosome | 0 | 8.3 | 0 | – | 2.9 | 0 | 0 | 0 | 0 | 10.2 | 10.6 | 13.2 | 10.9 | 9.0 | 9.1 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 |
Mitochondria | 0 | -4.8 | 0 | – | 2.6 | 6.4 | 3.5 | 4.3 | 5.1 | -6.3 | -7.4 | -13.3 | -8.0 | -9.2 | -9.9 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 2.7 | 3.5 | 3.0 | 4.1 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 3.4 | 0 | – | 5.9 | 5.8 | 6.3 | 5.8 | 5.8 | 0 | 4.6 | 5.0 | 7.4 | 6.2 | 5.6 |
Peroxisomes | 0 | 7.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.7 | 6.9 | 6.8 | 0 | 0 | 0 |
SpindlePole | 0 | 2.5 | 0 | – | -2.8 | 0 | -3.4 | -4.0 | 0 | 3.1 | 2.4 | 4.6 | 2.7 | 2.4 | 1.1 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 1.9 | 0 | 2.4 | 0 | 0 | 0 | 0 | 4.8 | 4.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2984 | 2.5643 | 2.1293 | 1.866 | 1.2352 | 2.1017 | 0.4001 | 1.1189 | 0.9155 | 0.5843 | 0.9187 | 1.0454 | 1.3145 | 2.4091 | 2.0254 | 1.8825 | 2.0155 | 2.2372 |
Actin | 0.0061 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.021 | 0.0005 | 0.01 | 0.0014 | 0.0167 | 0.0031 | 0.0146 | 0.0017 | 0.0018 | 0.0055 | 0.0001 | 0.0068 |
Bud | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0052 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0008 | 0.0009 | 0.0008 | 0.0007 | 0.0003 | 0.001 | 0.0008 | 0.0017 | 0.001 | 0.0008 | 0.0012 | 0.0001 | 0.0013 |
Bud Periphery | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0009 | 0.0001 | 0.0089 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 |
Bud Site | 0.0001 | 0.0014 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0039 | 0.0049 | 0.0139 | 0.0004 | 0.0173 | 0.0005 | 0.0005 | 0.0082 | 0.0004 | 0.0009 | 0.0001 | 0.0007 |
Cell Periphery | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0001 | 0.0009 | 0.0004 | 0.0003 | 0.0047 | 0.0006 | 0.0024 | 0.0028 | 0.0034 | 0.003 | 0.032 | 0.0028 | 0.0019 | 0.0053 | 0.0032 | 0.0009 | 0.0008 | 0.0042 |
Cytoplasmic Foci | 0.0504 | 0.0838 | 0.0703 | 0.0981 | 0.0901 | 0.0485 | 0.0932 | 0.128 | 0.1057 | 0.1529 | 0.1379 | 0.0929 | 0.1287 | 0.1213 | 0.1164 | 0.1516 | 0.0806 | 0.0864 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0014 | 0.0007 | 0.0027 | 0.0005 | 0.0028 | 0.0014 | 0.0074 | 0.0007 | 0.0009 | 0.0005 | 0.0011 | 0.0024 |
Endosome | 0.0631 | 0.0989 | 0.0674 | 0.0933 | 0.035 | 0.0508 | 0.1326 | 0.1548 | 0.1337 | 0.171 | 0.1873 | 0.0922 | 0.0884 | 0.1253 | 0.123 | 0.1168 | 0.0821 | 0.0859 |
Golgi | 0.0297 | 0.0359 | 0.0332 | 0.047 | 0.0283 | 0.0279 | 0.0374 | 0.0342 | 0.0427 | 0.0734 | 0.0981 | 0.0276 | 0.0192 | 0.0357 | 0.0376 | 0.0355 | 0.0295 | 0.0341 |
Lipid Particles | 0.7709 | 0.6929 | 0.7365 | 0.6605 | 0.6388 | 0.781 | 0.5793 | 0.5342 | 0.5441 | 0.4115 | 0.3156 | 0.6217 | 0.5875 | 0.5857 | 0.5434 | 0.4471 | 0.6468 | 0.6506 |
Mitochondria | 0.0029 | 0.0031 | 0.0042 | 0.0078 | 0.0014 | 0.0046 | 0.0095 | 0.0015 | 0.0066 | 0.0156 | 0.0397 | 0.0168 | 0.0014 | 0.0033 | 0.0035 | 0.005 | 0.0028 | 0.0084 |
None | 0.0001 | 0.0004 | 0.006 | 0.0006 | 0.0067 | 0.0018 | 0.0056 | 0.0059 | 0.0113 | 0.0005 | 0.0107 | 0.0082 | 0.0158 | 0.0039 | 0.0091 | 0.0007 | 0.0003 | 0.0076 |
Nuclear Periphery | 0.0054 | 0.0066 | 0.0011 | 0.0007 | 0.0063 | 0.005 | 0.0089 | 0.0016 | 0.0056 | 0.0004 | 0.0037 | 0.0106 | 0.0036 | 0.0069 | 0.0051 | 0.0026 | 0.0013 | 0.0054 |
Nucleolus | 0.0014 | 0.0053 | 0.0114 | 0.0016 | 0.0081 | 0.0154 | 0.0068 | 0.0169 | 0.0014 | 0.0007 | 0.0008 | 0.0022 | 0.0087 | 0.0133 | 0.011 | 0.0029 | 0.0002 | 0.0114 |
Nucleus | 0.0001 | 0.0019 | 0.0006 | 0.0003 | 0.0011 | 0.0032 | 0.0083 | 0.0034 | 0.0007 | 0.0004 | 0.0013 | 0.0044 | 0.0016 | 0.0011 | 0.0021 | 0.0018 | 0.0001 | 0.0018 |
Peroxisomes | 0.0592 | 0.0368 | 0.0558 | 0.0796 | 0.1365 | 0.0371 | 0.0636 | 0.0642 | 0.1047 | 0.1228 | 0.1054 | 0.0838 | 0.0783 | 0.0585 | 0.1068 | 0.204 | 0.1502 | 0.0767 |
Punctate Nuclear | 0.0059 | 0.0146 | 0.0038 | 0.0066 | 0.0403 | 0.01 | 0.009 | 0.0327 | 0.0044 | 0.039 | 0.0061 | 0.0236 | 0.0356 | 0.0122 | 0.0229 | 0.0151 | 0.0017 | 0.0117 |
Vacuole | 0.0019 | 0.0112 | 0.0048 | 0.0013 | 0.0008 | 0.0074 | 0.012 | 0.0106 | 0.0047 | 0.0044 | 0.0062 | 0.0053 | 0.0034 | 0.0126 | 0.0085 | 0.0048 | 0.0013 | 0.0028 |
Vacuole Periphery | 0.0021 | 0.0043 | 0.0024 | 0.0015 | 0.0007 | 0.0049 | 0.0025 | 0.002 | 0.0019 | 0.0015 | 0.0028 | 0.0019 | 0.0011 | 0.0024 | 0.0031 | 0.0022 | 0.0008 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.5062 | 3.8343 | 9.9583 | 14.4988 | 5.5612 | 6.4303 | 9.9345 | 13.7373 | 18.6098 | 13.0551 |
Translational Efficiency | 0.779 | 0.832 | 0.5083 | 0.5457 | 0.6062 | 1.4641 | 0.7571 | 0.6586 | 0.688 | 0.494 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1282 | 1317 | 601 | 721 | 1331 | 1570 | 2376 | 1610 | 2613 | 2887 | 2977 | 2331 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 650.66 | 737.67 | 1035.56 | 919.17 | 700.57 | 771.44 | 855.56 | 900.66 | 676.08 | 756.03 | 891.90 | 906.39 |
Standard Deviation | 76.51 | 108.97 | 138.04 | 149.87 | 84.38 | 94.74 | 101.74 | 142.92 | 84.39 | 102.86 | 131.64 | 145.36 |
Intensity Change Log 2 | — | 0.181072 | 0.670435 | 0.498428 | — | 0.139025 | 0.288340 | 0.362453 | — | 0.159425 | 0.484968 | 0.429530 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000741 | 0.002395 | 0.005028 | 0.011884 | 0.001191 | 0.001107 | 0.013384 | 0.011659 | 0.000970 | 0.001695 | 0.011697 | 0.011729 |
Bud Neck | 0.003750 | 0.001674 | 0.010809 | 0.003098 | 0.002664 | 0.002563 | 0.001608 | 0.002839 | 0.003197 | 0.002158 | 0.003466 | 0.002919 |
Bud Site | 0.001003 | 0.001665 | 0.016216 | 0.011407 | 0.001502 | 0.001459 | 0.004025 | 0.009865 | 0.001257 | 0.001553 | 0.006486 | 0.010342 |
Cell Periphery | 0.000153 | 0.000077 | 0.000615 | 0.000088 | 0.000098 | 0.000076 | 0.000052 | 0.000076 | 0.000125 | 0.000077 | 0.000166 | 0.000080 |
Cytoplasm | 0.021568 | 0.007706 | 0.108534* | 0.005947 | 0.018223 | 0.008639 | 0.004112 | 0.011593 | 0.019864 | 0.008213 | 0.025193 | 0.009847 |
Cytoplasmic Foci | 0.177957 | 0.226040* | 0.095914 | 0.274341* | 0.191287* | 0.189757* | 0.316693* | 0.288636* | 0.184747* | 0.206309* | 0.272122* | 0.284214* |
Eisosomes | 0.000271 | 0.000100 | 0.000115 | 0.000078 | 0.000187 | 0.000156 | 0.000063 | 0.000083 | 0.000228 | 0.000130 | 0.000073 | 0.000081 |
Endoplasmic Reticulum | 0.001413 | 0.001117 | 0.138128* | 0.002807 | 0.001212 | 0.001469 | 0.000755 | 0.001359 | 0.001311 | 0.001309 | 0.028488 | 0.001807 |
Endosome | 0.064530 | 0.174675 | 0.079593 | 0.279050* | 0.095562 | 0.152185 | 0.240065* | 0.297598* | 0.080337 | 0.162445 | 0.207669* | 0.291861* |
Golgi | 0.110944* | 0.182324* | 0.004497 | 0.067542 | 0.102279 | 0.145849* | 0.068292 | 0.081001 | 0.106530 | 0.162488* | 0.055413 | 0.076838 |
Lipid Particles | 0.505980* | 0.297258* | 0.155231* | 0.082197 | 0.476965* | 0.381569* | 0.118471* | 0.090851 | 0.491200* | 0.343108* | 0.125892* | 0.088174 |
Mitochondria | 0.023195 | 0.019162 | 0.000370 | 0.003519 | 0.011728 | 0.028358 | 0.005736 | 0.005760 | 0.017354 | 0.024163 | 0.004653 | 0.005067 |
Mitotic Spindle | 0.003465 | 0.026041 | 0.045318 | 0.126630* | 0.004964 | 0.014310 | 0.093958 | 0.081351 | 0.004229 | 0.019661 | 0.084138 | 0.095356 |
None | 0.001531 | 0.000688 | 0.000315 | 0.000058 | 0.001437 | 0.000490 | 0.000056 | 0.000107 | 0.001483 | 0.000580 | 0.000108 | 0.000092 |
Nuclear Periphery | 0.000729 | 0.000545 | 0.011156 | 0.004053 | 0.000697 | 0.000944 | 0.000408 | 0.001076 | 0.000713 | 0.000762 | 0.002578 | 0.001997 |
Nuclear Periphery Foci | 0.002249 | 0.005728 | 0.209025* | 0.025783 | 0.004537 | 0.005885 | 0.006309 | 0.011736 | 0.003415 | 0.005813 | 0.047233 | 0.016081 |
Nucleolus | 0.002508 | 0.002648 | 0.001104 | 0.002232 | 0.003955 | 0.002794 | 0.000886 | 0.001792 | 0.003245 | 0.002728 | 0.000930 | 0.001928 |
Nucleus | 0.004359 | 0.001793 | 0.036044 | 0.003034 | 0.005037 | 0.003114 | 0.001859 | 0.003068 | 0.004705 | 0.002511 | 0.008760 | 0.003058 |
Peroxisomes | 0.069171 | 0.040725 | 0.006019 | 0.056134 | 0.072027 | 0.050450 | 0.114094* | 0.067239 | 0.070626 | 0.046013 | 0.092276 | 0.063804 |
Vacuole | 0.003502 | 0.005495 | 0.075380 | 0.034973 | 0.003732 | 0.006519 | 0.008382 | 0.029857 | 0.003619 | 0.006052 | 0.021907 | 0.031439 |
Vacuole Periphery | 0.000980 | 0.002145 | 0.000588 | 0.005144 | 0.000714 | 0.002307 | 0.000791 | 0.002455 | 0.000844 | 0.002233 | 0.000750 | 0.003286 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.53 | -3.97 | -5.44 | -4.39 | -2.79 | 0.26 | -10.51 | -6.60 | -6.65 | 1.04 | -2.17 | -11.36 | -8.54 | -7.71 | 0.16 |
Bud Neck | 2.76 | -5.28 | 0.70 | -2.46 | 5.85 | 0.15 | 1.78 | -0.94 | -1.41 | -3.95 | 2.22 | -1.01 | -0.04 | -2.79 | 1.17 |
Bud Site | -1.32 | -8.60 | -5.63 | -5.20 | 1.88 | 0.10 | -3.30 | -6.40 | -7.01 | -4.68 | -0.65 | -8.48 | -8.78 | -8.70 | -3.48 |
Cell Periphery | 2.18 | -7.53 | 2.00 | -0.63 | 9.55 | 1.46 | 3.06 | 0.96 | -0.52 | -2.50 | 2.61 | -2.22 | 2.24 | -0.72 | 5.70 |
Cytoplasm | 5.40 | -11.56 | 5.21 | 0.27 | 13.55 | 4.17 | 6.20 | 0.83 | -3.48 | -5.69 | 6.80 | -2.79 | 3.21 | -3.13 | 5.74 |
Cytoplasmic Foci | -6.26 | 10.61 | -9.27 | -4.68 | -17.66 | 0.44 | -17.49 | -12.43 | -13.79 | 4.60 | -3.90 | -14.73 | -16.23 | -13.49 | -1.61 |
Eisosomes | 6.22 | 5.15 | 7.10 | 1.68 | 2.75 | 1.18 | 6.26 | 5.02 | 3.94 | -2.99 | 5.07 | 9.28 | 8.63 | 4.20 | -1.26 |
Endoplasmic Reticulum | 0.42 | -14.10 | -1.71 | -2.38 | 13.90 | -0.74 | 1.10 | -0.75 | 0.07 | -2.10 | -0.03 | -12.18 | -1.55 | -2.00 | 11.92 |
Endosome | -17.25 | -2.57 | -22.90 | -9.68 | -20.40 | -9.03 | -23.57 | -26.95 | -17.97 | -6.84 | -18.37 | -26.82 | -36.82* | -20.03 | -12.54 |
Golgi | -10.41 | 23.69 | 8.15 | 18.64 | -17.73 | -7.02 | 7.51 | 4.84 | 12.33 | -2.84 | -12.12 | 14.93 | 8.61 | 21.14 | -6.78 |
Lipid Particles | 16.26 | 26.69 | 38.05* | 20.86 | 6.19 | 7.49 | 35.76* | 38.34* | 30.50 | 4.87 | 16.33 | 48.20 | 54.23 | 36.41 | 7.36 |
Mitochondria | 1.32 | 10.04 | 7.92 | 7.50 | -3.06 | -6.02 | 3.68 | 3.61 | 9.21 | 0.07 | -3.38 | 8.68 | 8.27 | 11.63 | -0.38 |
Mitotic Spindle | -8.28 | -6.80 | -13.96 | -11.04 | -7.77 | -5.00 | -21.10* | -16.31 | -13.62 | 1.92 | -9.57 | -22.23 | -21.44 | -17.01 | -2.10 |
None | 1.94 | 2.90 | 3.48 | 3.64 | 5.08 | 2.37 | 3.36 | 3.17 | 2.97 | -1.97 | 3.08 | 4.66 | 4.64 | 4.52 | -0.14 |
Nuclear Periphery | 0.68 | -7.19 | -2.70 | -2.87 | 3.71 | -0.92 | 1.83 | -1.91 | -0.70 | -3.25 | -0.29 | -5.77 | -3.19 | -3.03 | 1.02 |
Nuclear Periphery Foci | -3.90 | -19.84 | -7.10 | -6.06 | 16.47 | -0.88 | -1.88 | -4.90 | -4.25 | -3.46 | -3.13 | -16.41 | -8.93 | -7.34 | 10.01 |
Nucleolus | -0.09 | 2.69 | 0.19 | 0.24 | -1.49 | 1.35 | 3.46 | 2.05 | 0.80 | -1.55 | 1.04 | 4.30 | 1.79 | 0.83 | -2.09 |
Nucleus | 2.86 | -9.33 | 0.70 | -1.56 | 9.43 | 1.91 | 3.16 | 1.11 | -0.67 | -1.88 | 3.22 | -4.42 | 1.17 | -1.61 | 5.09 |
Peroxisomes | 6.66 | 18.39 | 2.25 | -2.67 | -9.92 | 4.90 | -7.62 | 1.15 | -3.47 | 8.53 | 8.02 | -5.07 | 1.99 | -4.81 | 6.35 |
Vacuole | -1.85 | -14.93 | -9.77 | -9.21 | 6.33 | -3.04 | -5.84 | -13.29 | -12.05 | -10.94 | -3.44 | -14.47 | -16.47 | -15.23 | -6.07 |
Vacuole Periphery | -3.49 | 2.62 | -3.36 | -2.32 | -3.70 | -6.21 | -0.55 | -5.26 | -0.25 | -5.03 | -6.70 | 0.79 | -5.45 | -2.09 | -5.67 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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