Standard name
Human Ortholog
Description Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.06 0.14 0.07 0 0 – 0 0 0 0 0 0.06 0 0 0.09 0.07 0.13 0 0 0.05 0.07 0.05 0
Endoplasmic Reticulum 0 0.07 0.14 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.11 0.38 0 0.07 0.34 0.06 – 0.05 0 0 0 0 0.48 0.48 0.62 0.64 0.45 0.43 0.48 0.54 0.39 0.37 0.37 0.42
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.09 0.05 0.06 0 0 0.05 0.06
Mitochondria 0.68 0.42 0.73 0.71 0.51 0.86 – 0.82 0.92 0.85 0.88 0.89 0.4 0.39 0.16 0.12 0.11 0.15 0.16 0.1 0.25 0.22 0.21 0.18
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 – 0.08 0.11 0.09 0.14 0.13 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0.07 0.12 – 0.19 0.19 0.21 0.19 0.19 0 0.13 0.14 0.33 0.23 0.19 0 0 0 0 0 0
Peroxisomes 0 0.09 0 0.18 0.29 0.09 – 0 0 0 0 0 0.14 0.25 0.24 0 0.11 0.07 0.12 0.13 0.14 0.09 0.09 0.13
SpindlePole 0.15 0.09 0.08 0.15 0.17 0.05 – 0 0 0 0 0 0.2 0.16 0.26 0.22 0.19 0.13 0.06 0.05 0.06 0.11 0.06 0.06
Vac/Vac Membrane 0.05 0.12 0 0 0 0 – 0 0 0.05 0 0.06 0 0 0 0.12 0.18 0.13 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 5 1 1 2 0 – 1 0 1 0 0 5 10 1 0 1 1 7 15 1 5 15 16
Bud 13 5 4 15 11 9 – 10 6 8 5 10 2 4 1 0 1 0 1 1 0 1 1 3
Bud Neck 1 0 2 0 0 0 – 2 0 1 0 0 0 1 0 0 0 0 2 0 0 0 3 2
Bud Site 3 0 0 3 2 1 – 1 3 6 3 3 0 3 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 50 17 22 20 14 10 – 7 8 15 9 11 13 9 17 5 6 18 18 1 9 13 21 12
Endoplasmic Reticulum 17 20 22 0 4 4 – 6 1 3 2 1 0 2 1 0 3 6 3 2 0 2 12 2
Endosome 47 112 0 20 133 28 – 42 1 12 4 2 111 212 269 37 37 58 236 195 75 64 145 173
Golgi 2 1 0 0 0 0 – 0 0 0 0 2 2 4 8 1 5 12 27 21 4 3 19 25
Mitochondria 279 123 112 213 199 413 – 650 561 670 354 562 92 172 68 7 9 20 80 37 48 38 84 76
Nucleus 4 0 2 1 0 2 – 7 6 13 6 11 1 1 3 1 0 4 1 0 0 0 2 0
Nuclear Periphery 4 7 3 5 11 36 – 64 69 71 57 83 1 15 14 4 4 5 5 3 1 1 5 3
Nucleolus 17 6 0 14 28 58 – 152 115 169 77 119 6 57 63 19 19 25 3 2 0 0 1 0
Peroxisomes 13 27 0 55 113 44 – 17 4 6 6 3 31 113 107 1 9 9 57 48 27 14 34 54
SpindlePole 60 27 13 46 66 24 – 27 10 22 6 8 46 73 115 13 16 17 29 19 11 19 25 26
Vac/Vac Membrane 22 34 2 0 4 7 – 22 22 37 15 38 5 7 7 7 15 18 14 7 8 5 18 13
Unique Cell Count 412 291 153 301 394 483 789 611 791 401 630 229 446 437 58 83 135 495 362 194 175 396 416
Labelled Cell Count 541 384 183 393 587 636 1009 806 1034 544 853 315 683 675 95 125 193 495 362 194 175 396 416


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.6 3.5 3.8 5.1 4.7 – 5.5 5.3 5.6 5.4 5.2 5.4 6.5 7.3 8.9 9.3 9.1 5.0 5.4 5.6
Std Deviation (1e-4) 0.8 0.9 0.6 1.2 1.2 1.0 – 1.2 1.5 1.6 1.5 1.4 0.9 1.2 1.7 2.3 2.5 2.2 2.0 1.6 1.5
Intensity Change (Log2) – – – 0.12 0.54 0.42 – 0.64 0.6 0.69 0.63 0.59 0.62 0.89 1.06 1.34 1.41 1.39 0.52 0.63 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -4.6 -6.1 – 0 -7.4 -7.3 -5.6 -7.0 -2.9 -6.0 -4.5 -1.1 -1.6 -0.3
Endoplasmic Reticulum 0 -6.6 0 – 0 0 0 -7.2 0 -5.9 -7.6 -7.8 -3.1 -2.6 -2.8
Endosome 0 8.3 0 – 2.9 0 0 0 0 10.2 10.6 13.2 10.9 9.0 9.1
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3.8
Mitochondria 0 -4.8 0 – 2.6 6.4 3.5 4.3 5.1 -6.3 -7.4 -13.3 -8.0 -9.2 -9.9
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 2.7 3.5 3.0 4.1 4.0 0 0 0 0 0 0
Nucleolus 0 3.4 0 – 5.9 5.8 6.3 5.8 5.8 0 4.6 5.0 7.4 6.2 5.6
Peroxisomes 0 7.4 0 – 0 0 0 0 0 4.7 6.9 6.8 0 0 0
SpindlePole 0 2.5 0 – -2.8 0 -3.4 -4.0 0 3.1 2.4 4.6 2.7 2.4 1.1
Vacuole 0 0 0 – 0 0 1.9 0 2.4 0 0 0 0 4.8 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2984 2.5643 2.1293 1.866 1.2352 2.1017 0.4001 1.1189 0.9155 0.5843 0.9187 1.0454 1.3145 2.4091 2.0254 1.8825 2.0155 2.2372
Actin 0.0061 0.0006 0.0001 0.0002 0.0002 0.0003 0.021 0.0005 0.01 0.0014 0.0167 0.0031 0.0146 0.0017 0.0018 0.0055 0.0001 0.0068
Bud 0 0.0002 0 0 0 0 0.0005 0.0001 0.0007 0.0001 0.0052 0.0001 0.0001 0.0001 0 0.0002 0 0.0001
Bud Neck 0.0001 0.0005 0.0002 0.0003 0.0001 0.0008 0.0009 0.0008 0.0007 0.0003 0.001 0.0008 0.0017 0.001 0.0008 0.0012 0.0001 0.0013
Bud Periphery 0 0.0002 0.0001 0 0 0.0001 0.0008 0.0001 0.0009 0.0001 0.0089 0.0001 0.0002 0.0001 0.0001 0.0004 0 0.0002
Bud Site 0.0001 0.0014 0.001 0.0001 0.0001 0.0003 0.0039 0.0049 0.0139 0.0004 0.0173 0.0005 0.0005 0.0082 0.0004 0.0009 0.0001 0.0007
Cell Periphery 0 0.0002 0.0001 0 0 0.0002 0.0004 0.0001 0.0003 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0001
Cytoplasm 0.0001 0.0009 0.0004 0.0003 0.0047 0.0006 0.0024 0.0028 0.0034 0.003 0.032 0.0028 0.0019 0.0053 0.0032 0.0009 0.0008 0.0042
Cytoplasmic Foci 0.0504 0.0838 0.0703 0.0981 0.0901 0.0485 0.0932 0.128 0.1057 0.1529 0.1379 0.0929 0.1287 0.1213 0.1164 0.1516 0.0806 0.0864
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0001 0.0006 0.0002 0.0002 0.0007 0.0003 0.0014 0.0007 0.0027 0.0005 0.0028 0.0014 0.0074 0.0007 0.0009 0.0005 0.0011 0.0024
Endosome 0.0631 0.0989 0.0674 0.0933 0.035 0.0508 0.1326 0.1548 0.1337 0.171 0.1873 0.0922 0.0884 0.1253 0.123 0.1168 0.0821 0.0859
Golgi 0.0297 0.0359 0.0332 0.047 0.0283 0.0279 0.0374 0.0342 0.0427 0.0734 0.0981 0.0276 0.0192 0.0357 0.0376 0.0355 0.0295 0.0341
Lipid Particles 0.7709 0.6929 0.7365 0.6605 0.6388 0.781 0.5793 0.5342 0.5441 0.4115 0.3156 0.6217 0.5875 0.5857 0.5434 0.4471 0.6468 0.6506
Mitochondria 0.0029 0.0031 0.0042 0.0078 0.0014 0.0046 0.0095 0.0015 0.0066 0.0156 0.0397 0.0168 0.0014 0.0033 0.0035 0.005 0.0028 0.0084
None 0.0001 0.0004 0.006 0.0006 0.0067 0.0018 0.0056 0.0059 0.0113 0.0005 0.0107 0.0082 0.0158 0.0039 0.0091 0.0007 0.0003 0.0076
Nuclear Periphery 0.0054 0.0066 0.0011 0.0007 0.0063 0.005 0.0089 0.0016 0.0056 0.0004 0.0037 0.0106 0.0036 0.0069 0.0051 0.0026 0.0013 0.0054
Nucleolus 0.0014 0.0053 0.0114 0.0016 0.0081 0.0154 0.0068 0.0169 0.0014 0.0007 0.0008 0.0022 0.0087 0.0133 0.011 0.0029 0.0002 0.0114
Nucleus 0.0001 0.0019 0.0006 0.0003 0.0011 0.0032 0.0083 0.0034 0.0007 0.0004 0.0013 0.0044 0.0016 0.0011 0.0021 0.0018 0.0001 0.0018
Peroxisomes 0.0592 0.0368 0.0558 0.0796 0.1365 0.0371 0.0636 0.0642 0.1047 0.1228 0.1054 0.0838 0.0783 0.0585 0.1068 0.204 0.1502 0.0767
Punctate Nuclear 0.0059 0.0146 0.0038 0.0066 0.0403 0.01 0.009 0.0327 0.0044 0.039 0.0061 0.0236 0.0356 0.0122 0.0229 0.0151 0.0017 0.0117
Vacuole 0.0019 0.0112 0.0048 0.0013 0.0008 0.0074 0.012 0.0106 0.0047 0.0044 0.0062 0.0053 0.0034 0.0126 0.0085 0.0048 0.0013 0.0028
Vacuole Periphery 0.0021 0.0043 0.0024 0.0015 0.0007 0.0049 0.0025 0.002 0.0019 0.0015 0.0028 0.0019 0.0011 0.0024 0.0031 0.0022 0.0008 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5062 3.8343 9.9583 14.4988 5.5612 6.4303 9.9345 13.7373 18.6098 13.0551
Translational Efficiency 0.779 0.832 0.5083 0.5457 0.6062 1.4641 0.7571 0.6586 0.688 0.494

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Rrt8

Rrt8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrt8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available