Standard name
Human Ortholog
Description Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.99 0.95 0.98 0.94 0.87 0.81 0.77 0.68 0.68 0.59 0.61 0.99 1.0 0.99 0.94 0.95 0.96 0.94 0.93 0.92 0.87 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.05 0.08 0.1 0.32 0.37 0.36 0.5 0.55 0.63 0.59 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 3
Bud 1 0 0 0 2 0 3 5 6 5 3 1 0 0 1 1 0 0 0 0 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 2 1 1 4 1 10 5 0 10 0 2 0 14 14 13 0 0 0 0 0 0
Cytoplasm 230 189 191 178 197 289 292 282 185 199 119 170 217 255 278 404 379 318 235 186 173 70 155 171
Endoplasmic Reticulum 3 2 3 1 0 0 2 3 3 1 2 3 0 0 1 17 20 16 0 0 0 0 1 2
Endosome 0 0 0 0 2 0 0 4 1 0 0 0 0 0 0 9 5 1 3 0 0 0 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 2 3 2 1 0 0 3
Mitochondria 25 1 10 14 21 106 132 130 136 161 128 165 2 1 1 14 4 6 2 0 4 2 2 2
Nucleus 1 0 0 1 0 2 2 3 5 4 2 6 0 0 0 6 6 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 2 1 3 1 2 0 1 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 0 2 0 2 0 1 3
Vac/Vac Membrane 0 0 1 1 2 10 7 11 13 16 3 22 0 0 1 9 18 4 0 6 0 0 0 5
Unique Cell Count 245 191 201 182 209 333 359 366 272 293 202 280 220 256 280 428 400 330 250 199 188 81 171 201
Labelled Cell Count 260 192 207 197 225 408 446 441 362 394 258 379 220 259 282 479 454 366 250 199 188 81 171 201


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.3 4.6 4.8 5.0 4.5 4.1 4.5 4.3 4.0 4.2 4.2 5.7 5.6 5.6 5.9 7.0 7.1 4.8 5.5 6.1
Std Deviation (1e-4) 0.6 0.7 1.0 0.8 1.2 1.0 1.1 1.2 1.0 1.1 1.1 1.1 0.9 0.8 1.1 1.5 1.8 1.9 1.3 2.0 1.5
Intensity Change (Log2) – – – 0.08 0.14 -0.01 -0.16 -0.03 -0.09 -0.2 -0.11 -0.13 0.33 0.29 0.31 0.37 0.63 0.63 0.08 0.28 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2 2.4
Cytoplasm 1.4 -0.3 -3.1 -4.5 -5.5 -7.2 -7.3 -8.6 -8.6 2.1 3.2 3.0 -0.3 -0.1 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.7 2.1 2.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.9 0 0 0 0 0 0 0 -2.5 0 0 -1.0 0 -2.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 2.7 3.0 0 3.7 0 0 0 0 2.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0485 3.0777 2.1781 1.8875 1.8512 2.2411 3.9812 4.97 4.7641 4.3912 4.264 4.7159 5.0758 6.7132 6.0492 5.9767 5.0334 5.8135
Actin 0.021 0.008 0.0028 0.001 0.044 0.0118 0.0188 0.0041 0.0049 0.0016 0.0385 0.0034 0.021 0.0016 0.0279 0.0314 0.0061 0.0116
Bud 0.0005 0.0003 0.0004 0.0001 0.0014 0.0004 0.0022 0.0009 0.0025 0.0005 0.0009 0.0016 0.0079 0.0059 0.0004 0.005 0.0003 0.0001
Bud Neck 0.0022 0.0002 0.0003 0.0003 0.0016 0.0008 0.0025 0.0003 0.0015 0.0003 0.0022 0.0011 0.0008 0.0002 0.0005 0.0017 0.0004 0.0005
Bud Periphery 0.0009 0.0003 0.0003 0 0.0019 0.0005 0.0024 0.0009 0.0024 0.0003 0.0015 0.0009 0.0048 0.0074 0.0005 0.0029 0.0005 0
Bud Site 0.0061 0.0022 0.0017 0.0001 0.007 0.0007 0.0073 0.0042 0.0052 0.0003 0.0054 0.001 0.0103 0.0089 0.0008 0.0016 0.0003 0
Cell Periphery 0.0008 0.0003 0.0003 0 0.0004 0.0001 0.0004 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0
Cytoplasm 0.6394 0.8891 0.7717 0.8595 0.6021 0.882 0.7034 0.8191 0.8274 0.8181 0.7599 0.8468 0.7176 0.9214 0.8669 0.8517 0.8561 0.9362
Cytoplasmic Foci 0.0416 0.0041 0.0182 0.0081 0.036 0.0093 0.0379 0.019 0.0143 0.0074 0.0146 0.0125 0.0244 0.0027 0.0134 0.0135 0.0061 0.0035
Eisosomes 0.0004 0.0002 0.0002 0.0001 0.0008 0.0001 0.0001 0 0.0001 0 0.0003 0 0.0002 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0102 0.0034 0.0042 0.0046 0.0099 0.0038 0.0051 0.0014 0.0021 0.002 0.0026 0.0015 0.008 0.0012 0.0025 0.0026 0.0027 0.0018
Endosome 0.0536 0.0031 0.0052 0.008 0.0323 0.0174 0.0173 0.0028 0.0063 0.0031 0.0088 0.0057 0.0351 0.0006 0.0021 0.0083 0.0301 0.0014
Golgi 0.0085 0.0017 0.0013 0.0003 0.0093 0.0102 0.0074 0.0007 0.0024 0.0004 0.007 0.002 0.0073 0.0002 0.0016 0.0034 0.0102 0.0002
Lipid Particles 0.0053 0.0011 0.0008 0.0001 0.0137 0.0013 0.0056 0.0002 0.0012 0 0.0089 0.0013 0.0133 0 0.0007 0.0034 0.0118 0
Mitochondria 0.0197 0.0076 0.0144 0.0002 0.004 0.0042 0.0023 0.0004 0.0029 0.0005 0.0028 0.0015 0.001 0.0001 0.0012 0.0015 0.0296 0.0001
None 0.161 0.0724 0.1587 0.1093 0.1757 0.0472 0.161 0.139 0.1148 0.1608 0.1305 0.1092 0.1107 0.0469 0.0727 0.0394 0.0341 0.0411
Nuclear Periphery 0.0029 0.0009 0.001 0.0015 0.0192 0.0022 0.0028 0.0006 0.0009 0.0008 0.0021 0.001 0.0095 0.0004 0.0007 0.0021 0.0032 0.0005
Nucleolus 0.0003 0.0002 0.0002 0.0001 0.0006 0.0001 0.0007 0.0001 0.0002 0 0.0004 0.0002 0.0003 0 0 0.0034 0.0002 0
Nucleus 0.0043 0.0023 0.0023 0.0031 0.012 0.0039 0.0035 0.0019 0.0026 0.002 0.0022 0.0037 0.0082 0.0012 0.0013 0.0042 0.0015 0.002
Peroxisomes 0.0141 0.0005 0.0136 0.0001 0.0137 0.0006 0.0125 0.0006 0.0049 0.0003 0.0071 0.0035 0.007 0 0.005 0.0018 0.0018 0.0001
Punctate Nuclear 0.0023 0.0007 0.0009 0.0016 0.0036 0.001 0.004 0.0026 0.002 0.0008 0.0025 0.0021 0.0108 0.0002 0.0012 0.0203 0.0006 0.0005
Vacuole 0.0038 0.0013 0.0012 0.0016 0.0079 0.0018 0.0023 0.0008 0.0011 0.0006 0.0011 0.0007 0.0013 0.0006 0.0003 0.0012 0.0018 0.0003
Vacuole Periphery 0.001 0.0002 0.0002 0.0002 0.003 0.0005 0.0005 0.0001 0.0002 0.0001 0.0004 0.0002 0.0005 0.0001 0.0001 0.0003 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.1984 53.4621 53.5561 84.9935 82.8708 62.7606 65.7112 68.4072 75.5819 76.8256
Translational Efficiency 1.5295 1.4262 1.3767 0.9478 0.7747 1.3338 1.2937 1.0494 1.1374 1.0471

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1289 127 1603 1957 109 545 454 23 1398 672 2057 1980

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.16 794.40 829.74 772.15 702.05 766.54 864.93 815.40 632.08 771.81 837.51 772.65
Standard Deviation 86.10 139.84 113.70 89.18 90.81 110.65 85.93 119.32 88.84 117.24 109.17 89.71
Intensity Change Log 2 — 0.343334 0.406128 0.302350 — 0.126787 0.301010 0.215934 — 0.232932 0.351521 0.257319

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000073 0.000234 0.000280 0.000191 0.000040 0.000121 0.000091 0.000237 0.000070 0.000142 0.000238 0.000192
Bud Neck 0.009999 0.021563 0.005069 0.003163 0.011799 0.004354 0.002626 0.004081 0.010139 0.007606 0.004530 0.003174
Bud Site 0.005244 0.040192 0.007819 0.008791 0.004743 0.010466 0.003766 0.005906 0.005205 0.016084 0.006925 0.008758
Cell Periphery 0.000201 0.000162 0.000126 0.000084 0.000213 0.000096 0.000110 0.000157 0.000202 0.000108 0.000122 0.000085
Cytoplasm 0.613397* 0.473044* 0.437752* 0.676230* 0.496380* 0.662116* 0.676377* 0.472596* 0.604274* 0.626384* 0.490419* 0.673865*
Cytoplasmic Foci 0.111603 0.167920 0.008354 0.032843 0.156926 0.124276 0.048416 0.081324 0.115137 0.132524 0.017196 0.033406
Eisosomes 0.000129 0.000079 0.000022 0.000030 0.000122 0.000063 0.000039 0.000055 0.000129 0.000066 0.000026 0.000031
Endoplasmic Reticulum 0.001290 0.000971 0.002876 0.000699 0.000915 0.000191 0.001277 0.000611 0.001260 0.000339 0.002523 0.000698
Endosome 0.005074 0.034891 0.001724 0.003946 0.015447 0.013880 0.004256 0.013080 0.005882 0.017851 0.002283 0.004052
Golgi 0.000702 0.001549 0.000089 0.000811 0.000267 0.004292 0.000082 0.083064 0.000668 0.003774 0.000087 0.001767
Lipid Particles 0.005088 0.007379 0.000920 0.000817 0.003564 0.002398 0.001500 0.000742 0.004969 0.003340 0.001048 0.000817
Mitochondria 0.002072 0.000932 0.000998 0.000612 0.000241 0.002273 0.000114 0.000260 0.001929 0.002020 0.000803 0.000608
Mitotic Spindle 0.000497 0.020027 0.005527 0.014767 0.002301 0.007209 0.001193 0.000128 0.000638 0.009631 0.004570 0.014597
None 0.008276 0.001189 0.007327 0.002944 0.000689 0.001947 0.004358 0.001646 0.007684 0.001804 0.006672 0.002928
Nuclear Periphery 0.000728 0.000506 0.001665 0.000790 0.000706 0.000154 0.000572 0.000255 0.000727 0.000220 0.001423 0.000783
Nuclear Periphery Foci 0.000609 0.001498 0.000909 0.000635 0.000483 0.000653 0.002447 0.001484 0.000599 0.000813 0.001249 0.000645
Nucleolus 0.000838 0.000547 0.000773 0.000342 0.000388 0.001001 0.000266 0.000547 0.000803 0.000915 0.000661 0.000344
Nucleus 0.179643* 0.054683 0.483909* 0.144067* 0.124420 0.044998 0.177446* 0.116488 0.175337* 0.046828 0.416269* 0.143747*
Peroxisomes 0.001756 0.003656 0.000375 0.000689 0.002792 0.007491 0.000235 0.000545 0.001836 0.006766 0.000344 0.000688
Vacuole 0.052097 0.165745 0.033150 0.106206 0.176837 0.111617 0.074687 0.212891* 0.061823 0.121847 0.042318 0.107445
Vacuole Periphery 0.000683 0.003233 0.000338 0.001341 0.000727 0.000404 0.000140 0.003902 0.000687 0.000939 0.000294 0.001371

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.74 -7.72 -2.60 1.85 3.76 -3.35 -5.16 -3.03 -1.62 -1.88 -3.34 -7.88 -2.76 -0.41 2.95
Bud Neck -1.40 3.55 5.84 2.42 2.33 1.47 1.79 1.82 1.71 -0.00 1.72 4.34 6.04 2.67 1.81
Bud Site -3.30 -1.23 -1.27 3.12 0.09 -1.67 0.48 -0.24 1.82 -1.27 -3.44 -0.89 -1.33 2.80 -0.45
Cell Periphery 1.36 5.53 8.82 2.97 4.96 2.70 2.19 2.23 -0.15 0.60 6.48 6.16 9.09 2.67 5.03
Cytoplasm 4.89 20.04 -0.80 -5.21 -22.24 -5.94 -5.61 1.99 5.53 5.34 -1.69 14.89 -1.50 0.64 -17.35
Cytoplasmic Foci -3.00 27.37 20.95 8.46 -15.64 2.25 7.75 5.65 5.19 -0.63 -2.13 26.23 22.42 17.51 -8.14
Eisosomes 4.21 13.19 12.52 4.31 -3.12 4.73 6.18 3.87 0.21 -1.30 7.94 13.54 13.32 7.87 -1.11
Endoplasmic Reticulum 0.67 -6.26 1.50 0.72 13.66 3.02 -1.75 0.45 -2.65 2.40 3.19 -5.64 1.51 -6.34 13.73
Endosome -2.77 7.59 3.72 2.98 -4.38 0.59 4.14 2.11 1.82 -0.53 -4.54 7.46 5.03 5.70 -2.49
Golgi -1.92 4.88 0.75 2.21 -2.18 -3.11 2.08 -0.99 -0.86 -1.00 -2.89 5.00 -0.45 2.53 -1.99
Lipid Particles -0.32 6.74 7.17 1.29 2.22 1.08 2.18 3.26 1.88 3.43 1.60 6.81 7.50 2.13 3.67
Mitochondria 1.86 1.83 2.61 1.06 2.88 -1.50 1.91 1.50 1.59 -0.01 0.05 2.08 2.57 1.34 2.00
Mitotic Spindle -1.75 -5.23 -6.17 0.61 -2.55 -1.47 0.01 0.95 2.96 1.76 -3.15 -5.16 -6.07 -0.85 -3.09
None 5.17 1.13 4.29 -2.87 7.06 -2.71 -2.75 -0.42 1.61 2.41 4.77 1.24 4.16 -2.12 6.45
Nuclear Periphery 0.61 -4.87 -1.63 -2.39 10.03 2.59 0.13 1.81 -3.00 5.64 2.36 -4.09 -1.73 -14.21 7.76
Nuclear Periphery Foci -1.72 -2.75 0.17 1.75 4.82 -1.17 -6.67 -2.03 -1.86 0.59 -1.51 -4.70 0.01 1.68 7.23
Nucleolus 0.80 0.61 3.85 1.38 2.59 -1.00 1.93 1.02 1.19 0.02 -0.18 1.11 3.87 1.20 2.44
Nucleus 10.82 -34.84 1.81 -10.18 38.66 4.61 -3.09 1.87 -1.51 4.95 22.18 -29.97 1.34 -24.10 33.03
Peroxisomes -2.00 3.45 2.82 4.08 -1.05 -1.57 1.23 1.17 3.37 -1.35 -2.72 3.73 2.99 3.57 -1.24
Vacuole -6.13 2.46 -17.30 1.29 -20.91 2.57 3.40 -3.90 -5.21 -5.55 -8.57 1.76 -15.40 -2.78 -18.87
Vacuole Periphery -1.51 3.38 -0.55 1.34 -1.67 1.55 2.83 -0.54 -0.81 -1.01 -0.59 4.14 -0.57 -0.06 -1.82
Standard name
Human Ortholog
Description Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
Localization
Cell Percentages cytoplasm (28%), nucleus (10%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gsh2

Gsh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available