Standard name
Human Ortholog
Description Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.95 0.87 0.93 0.91 0.92 – 0.63 0.71 0.62 0.67 0.65 0.97 0.96 0.9 0.63 0.6 0.71 0.67 0.4 0.31 0.52 0.68 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0.1 0.07 0.09 0.08 – 0.37 0.33 0.4 0.37 0.39 0 0 0 0.35 0.4 0.34 0.23 0.51 0.58 0.38 0.18 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 – 1 0 5 0 1 0 0 3 0 0 1 1 1 0 0 1 5
Bud 0 1 3 5 3 7 – 15 7 16 21 5 0 1 3 0 1 0 1 1 1 1 7 11
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 2 2 1 1 1 3
Bud Site 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 1 0 – 1 0 1 1 2 4 4 18 1 1 1 0 0 0 0 0 1
Cytoplasm 245 160 207 302 357 456 – 437 343 326 297 305 348 435 407 71 98 135 179 70 75 79 261 327
Endoplasmic Reticulum 2 0 0 0 11 5 – 4 1 2 2 7 7 14 37 5 3 3 0 2 3 1 4 1
Endosome 0 0 0 0 3 7 – 1 11 4 0 1 1 1 0 2 7 8 3 3 3 1 4 6
Golgi 0 0 0 0 0 1 – 1 0 0 0 1 0 0 1 1 0 3 0 0 0 2 2 0
Mitochondria 8 0 31 33 3 11 – 21 15 15 11 10 1 3 2 5 5 1 7 1 9 3 13 17
Nucleus 0 1 2 1 2 2 – 21 20 21 23 14 0 1 0 2 6 3 0 0 0 2 2 2
Nuclear Periphery 0 0 0 2 1 0 – 4 5 0 2 1 0 2 0 0 0 2 0 0 0 0 0 0
Nucleolus 0 0 0 2 0 1 – 2 1 1 1 3 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 1 0 0 0 – 0 0 3 0 0 0 0 1 0 0 0 0 0 1 0 4 9
Vac/Vac Membrane 3 19 24 22 34 42 – 257 158 208 165 185 4 2 2 40 65 64 63 90 141 57 70 84
Unique Cell Count 253 169 238 324 393 496 689 484 524 446 471 358 453 450 113 163 191 270 178 245 154 383 480
Labelled Cell Count 260 181 268 367 416 533 765 562 604 523 535 366 463 474 127 186 222 270 178 245 154 383 480


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.8 3.2 3.4 4.8 4.8 – 6.6 7.2 9.5 9.6 10.8 4.5 5.0 5.7 8.1 7.9 8.9 5.3 5.0 4.9
Std Deviation (1e-4) 0.8 1.2 0.8 1.5 1.6 1.6 – 2.7 4.1 5.8 7.3 7.5 2.0 1.5 1.8 2.8 3.3 3.8 10.1 4.8 5.7
Intensity Change (Log2) – – – 0.12 0.61 0.6 – 1.05 1.17 1.58 1.6 1.77 0.52 0.66 0.86 1.34 1.31 1.49 0.75 0.66 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.7 7.1 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2443 1.8665 2.0304 1.3966 2.4855 1.922 2.0018 2.5918 2.1987 0.9161 2.0125 1.748 3.8207 4.5019 3.8513 3.3052 4.0171 4.0793
Actin 0.0095 0.0001 0.0029 0.0087 0.0445 0.0006 0.0183 0.0001 0.0175 0 0.0008 0.003 0.0074 0 0.0001 0 0 0.0003
Bud 0.0055 0.0023 0.0016 0.0158 0.0019 0.0039 0.0006 0.0015 0.0016 0.0002 0.0008 0.0008 0.0007 0.0013 0.0008 0.0006 0.0004 0.0009
Bud Neck 0.0034 0.0017 0.0018 0.001 0.0028 0.0035 0.001 0.0011 0.0035 0.0004 0.0023 0.002 0.0011 0.0023 0.0019 0.0017 0.0016 0.0041
Bud Periphery 0.0066 0.0047 0.0025 0.0079 0.0044 0.0042 0.0013 0.0036 0.0032 0.0009 0.0019 0.0019 0.0015 0.0025 0.002 0.0009 0.0008 0.0034
Bud Site 0.0143 0.0176 0.005 0.0064 0.0068 0.0019 0.0021 0.0045 0.0042 0.0003 0.002 0.0014 0.0015 0.0077 0.0022 0.001 0.0015 0.0014
Cell Periphery 0.0037 0.0025 0.0029 0.0017 0.003 0.0039 0.0038 0.0138 0.0207 0.082 0.0014 0.0224 0.0082 0.0063 0.0023 0.001 0.001 0.0049
Cytoplasm 0.0799 0.1191 0.0731 0.0086 0.0312 0.0467 0.0341 0.0658 0.0313 0.0194 0.0081 0.0345 0.0741 0.0958 0.0553 0.0244 0.0166 0.066
Cytoplasmic Foci 0.0349 0.0321 0.0343 0.0247 0.0472 0.0348 0.038 0.0225 0.0332 0.0197 0.0064 0.0148 0.0309 0.0194 0.0348 0.0278 0.0184 0.0177
Eisosomes 0.0002 0 0.0001 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0489 0.0339 0.0413 0.0047 0.0463 0.027 0.0245 0.0131 0.0235 0.0011 0.0032 0.0088 0.0394 0.0254 0.0401 0.0026 0.0119 0.0227
Endosome 0.2334 0.1721 0.2275 0.2243 0.2506 0.233 0.2895 0.1704 0.2726 0.4484 0.1299 0.1891 0.2155 0.127 0.2314 0.2586 0.1915 0.1841
Golgi 0.0118 0.0027 0.0084 0.0101 0.012 0.005 0.0146 0.003 0.0136 0.012 0.0176 0.0091 0.0061 0.0018 0.0049 0.0041 0.0018 0.0042
Lipid Particles 0.0232 0.012 0.0211 0.0059 0.0261 0.0127 0.0244 0.0103 0.0144 0.0045 0.0479 0.0099 0.0163 0.0073 0.0121 0.0041 0.0073 0.01
Mitochondria 0.0354 0.0034 0.0243 0.0055 0.0289 0.0097 0.0175 0.002 0.0256 0.0023 0.0047 0.0026 0.0027 0.0025 0.0081 0.0048 0.0024 0.0036
None 0.0542 0.065 0.0564 0.0066 0.0312 0.0194 0.0088 0.0293 0.0312 0.0009 0.0002 0.0119 0.0481 0.0612 0.032 0.0478 0.0389 0.0374
Nuclear Periphery 0.0258 0.0206 0.0154 0.0055 0.0087 0.0154 0.0288 0.0121 0.0083 0.0011 0.0036 0.0075 0.0214 0.0094 0.0158 0.0035 0.0065 0.0136
Nucleolus 0.0046 0.0059 0.0057 0.0086 0.0061 0.0054 0.0045 0.005 0.0055 0.0011 0.0027 0.0026 0.003 0.0038 0.0038 0.0028 0.0043 0.0026
Nucleus 0.0109 0.0121 0.0185 0.0064 0.0095 0.0121 0.0154 0.0077 0.0134 0.0013 0.0051 0.0085 0.0104 0.0085 0.0076 0.0053 0.006 0.0137
Peroxisomes 0.0128 0.0009 0.0027 0.0015 0.0042 0.001 0.0048 0.001 0.0053 0.0005 0.0013 0.0015 0.002 0.0003 0.0009 0.0009 0.001 0.0006
Punctate Nuclear 0.0026 0.0015 0.0039 0.0043 0.0029 0.0064 0.0065 0.0008 0.0008 0.0001 0.0002 0.0004 0.0042 0.0005 0.0013 0.0015 0.0017 0.001
Vacuole 0.3303 0.4367 0.4002 0.5686 0.3874 0.4891 0.3981 0.568 0.3957 0.3284 0.6214 0.5984 0.4655 0.5794 0.4781 0.5304 0.6315 0.5489
Vacuole Periphery 0.048 0.0533 0.0504 0.0731 0.0441 0.0643 0.0634 0.0642 0.0748 0.0754 0.1383 0.0689 0.0398 0.0376 0.0644 0.0762 0.0547 0.0587

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
16 1789 819 100 1494 1380 2313 113 1510 3169 3132 213

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 329.44 725.57 928.73 857.47 601.75 728.40 762.54 870.76 598.86 726.80 806.00 864.52
Standard Deviation 2.99 83.15 139.52 140.58 222.29 86.31 92.00 140.30 222.86 84.55 129.13 140.59
Intensity Change Log 2 — 1.139099 1.495244 1.380070 — 0.275567 0.341649 0.533111 — 0.642850 0.860960 0.892148

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000035 0.000216 0.000704 0.000399 0.000098 0.000095 0.000234 0.000308 0.000097 0.000163 0.000357 0.000351
Bud Neck 0.000036 0.028342 0.003083 0.002292 0.019864 0.030524 0.010774 0.002154 0.019654 0.029292 0.008763 0.002219
Bud Site 0.000035 0.010341 0.008818 0.007392 0.003361 0.008117 0.018047 0.007788 0.003326 0.009372 0.015634 0.007602
Cell Periphery 0.000076 0.000123 0.000211 0.000449 0.000400 0.000208 0.000161 0.000216 0.000397 0.000160 0.000174 0.000325
Cytoplasm 0.000059 0.465195* 0.463886* 0.341096* 0.434275* 0.475543* 0.595044* 0.364958* 0.429674* 0.469701* 0.560747* 0.353755*
Cytoplasmic Foci 0.000125 0.192505* 0.035568 0.116339 0.250177* 0.183817* 0.024346 0.088283 0.247528* 0.188721* 0.027280 0.101454
Eisosomes 0.000053 0.000133 0.000059 0.000204 0.000296 0.000229 0.000028 0.000101 0.000293 0.000175 0.000036 0.000150
Endoplasmic Reticulum 0.000001 0.000415 0.044393 0.004962 0.000704 0.000466 0.002658 0.003678 0.000696 0.000437 0.013571 0.004281
Endosome 0.000007 0.027734 0.011326 0.019219 0.018435 0.020618 0.002320 0.012779 0.018239 0.024635 0.004675 0.015802
Golgi 0.000009 0.003835 0.000413 0.002795 0.001347 0.002853 0.000120 0.000298 0.001332 0.003407 0.000196 0.001470
Lipid Particles 0.000027 0.005942 0.005514 0.015247 0.017358 0.007507 0.000962 0.004856 0.017174 0.006624 0.002153 0.009734
Mitochondria 0.000014 0.004844 0.001730 0.000893 0.003254 0.006738 0.001069 0.002019 0.003220 0.005669 0.001242 0.001490
Mitotic Spindle 0.000337 0.006641 0.004542 0.001844 0.000784 0.003759 0.004183 0.002119 0.000779 0.005386 0.004277 0.001990
None 0.998870* 0.001872 0.003006 0.003023 0.006058 0.001603 0.002335 0.002067 0.016578 0.001755 0.002511 0.002516
Nuclear Periphery 0.000000 0.000422 0.015190 0.001517 0.000770 0.000425 0.000753 0.001593 0.000762 0.000423 0.004528 0.001558
Nuclear Periphery Foci 0.000096 0.000228 0.022146 0.009314 0.000424 0.000234 0.000723 0.004127 0.000420 0.000231 0.006325 0.006562
Nucleolus 0.000080 0.001358 0.000599 0.001173 0.002349 0.001190 0.000259 0.000690 0.002325 0.001285 0.000348 0.000917
Nucleus 0.000013 0.119766 0.257070* 0.244169* 0.163095* 0.152178* 0.296612* 0.278619* 0.161367* 0.133880* 0.286272* 0.262445*
Peroxisomes 0.000125 0.010859 0.000707 0.000995 0.007595 0.006985 0.000997 0.000597 0.007516 0.009172 0.000921 0.000784
Vacuole 0.000003 0.117683 0.120524 0.224531* 0.068608 0.095429 0.038066 0.220637* 0.067881 0.107992 0.059628 0.222465*
Vacuole Periphery 0.000000 0.001545 0.000508 0.002146 0.000748 0.001483 0.000309 0.002113 0.000740 0.001518 0.000361 0.002128

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.62 -2.76 -3.40 -1.92 0.79 0.15 -1.99 -1.81 -1.88 -0.39 -2.41 -3.14 -3.31 -2.51 -0.17
Bud Neck -16.13 -11.46 -4.28 13.68 0.81 -4.01 4.67 10.62 12.94 7.08 -4.63 6.03 10.45 19.00 6.84
Bud Site -9.72 -5.53 -2.51 0.49 -0.14 -3.20 -7.55 -1.24 0.18 2.73 -5.38 -7.67 -1.90 0.55 2.62
Cell Periphery -1.25 -3.52 -5.69 -6.07 -4.26 0.97 1.21 1.12 0.96 -0.50 1.22 1.14 0.49 -4.52 -3.97
Cytoplasm -68.81* -41.89 -14.08 6.24 4.55 -4.44 -17.32 3.90 5.70 10.39 -5.01 -13.75 5.63 8.35 12.75
Cytoplasmic Foci -40.84* -16.91 -10.32 7.09 -6.94 9.28 40.88 17.99 11.67 -4.77 9.48 40.29 18.41 13.11 -8.47
Eisosomes -5.74 -1.37 -7.41 -3.85 -8.07 1.29 5.94 4.32 4.82 -6.18 2.58 5.71 3.26 1.93 -10.43
Endoplasmic Reticulum -16.15 -7.96 -5.66 -5.19 6.69 1.67 -2.79 -2.53 -2.82 -0.21 2.00 -8.10 -5.11 -5.59 5.11
Endosome -15.79 -14.05 -6.00 2.73 -2.29 -1.09 13.09 3.50 4.16 -2.99 -3.61 11.16 2.24 4.99 -4.61
Golgi -7.15 -2.32 -1.13 0.92 -0.94 -2.64 10.74 7.42 4.73 -0.69 -5.08 9.73 0.33 2.47 -1.04
Lipid Particles -14.99 -12.32 -3.85 -2.22 -2.35 7.16 12.67 8.68 3.40 -3.85 8.09 11.72 3.84 -1.22 -3.70
Mitochondria -8.80 -2.63 -1.41 5.55 0.98 -2.70 4.12 2.19 3.84 -0.17 -3.15 3.95 3.31 6.01 0.33
Mitotic Spindle -4.23 -3.57 -1.40 2.92 2.05 -2.21 -3.69 -1.42 0.68 1.28 -4.11 -4.30 -1.49 2.61 2.32
None 1812.33* 1969.63* 654.77* -0.70 -0.20 3.95 3.45 3.83 -0.08 1.55 5.23 5.03 4.92 -0.66 0.16
Nuclear Periphery -12.14 -6.10 -5.45 -4.16 5.13 3.40 -0.41 -3.34 -5.14 -3.36 3.52 -5.64 -4.33 -6.37 3.80
Nuclear Periphery Foci -1.65 -8.50 -4.79 -4.73 3.10 2.42 -1.94 -3.54 -3.80 -3.11 2.98 -8.32 -5.58 -5.78 -0.67
Nucleolus -8.95 -7.38 -4.24 0.79 -2.14 3.15 5.83 5.03 3.21 -1.88 3.08 5.60 4.22 2.85 -3.30
Nucleus -33.92* -30.55 -10.96 -4.95 2.27 1.69 -20.42 -2.98 -3.42 2.74 5.24 -21.21 -4.13 -5.92 3.97
Peroxisomes -11.50 -4.38 -5.58 10.52 -1.50 0.64 7.27 8.05 7.35 2.10 -1.29 7.45 7.80 12.90 1.12
Vacuole -29.70* -22.93 -11.86 -6.69 -4.96 -5.73 6.39 -8.72 -7.78 -9.56 -9.95 -1.62 -12.20 -10.10 -11.92
Vacuole Periphery -11.92 -8.44 -1.55 -0.08 -1.11 -2.59 7.47 -0.52 0.29 -1.02 -5.14 6.99 -0.98 0.15 -1.56
Standard name
Human Ortholog
Description Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (13%), vacuole (7%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ddr2

Ddr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ddr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available