Standard name
Human Ortholog
Description Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM3 has a paralog, GPM2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 1.0 0.98 0.9 0.94 0.84 0.79 0.76 0.73 0.67 0.69 0.98 0.99 1.0 0.95 1.0 0.96 0.96 0.95 0.98 0.91 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0 0.16 0.09 0.33 0.3 0.43 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.15 0.06 0 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 3 0 4 0 2 1 5 4 0 0 0 0 0 0 0 1 0 3 12 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 0 0 0 1 4 3 0 0 4 1 0 3 1 2 1 1 0 0 0 0 0 0
Cytoplasm 318 472 23 111 91 173 124 168 72 98 140 155 317 380 450 83 122 70 315 466 72 190 363 285
Endoplasmic Reticulum 1 4 0 0 1 5 0 0 0 4 0 7 4 1 1 1 1 6 0 3 0 1 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 4 0 0 1 2
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 2 0 0 0 0
Mitochondria 2 3 0 0 5 2 23 20 31 40 89 68 1 2 0 3 1 0 1 4 0 3 1 3
Nucleus 2 6 0 2 3 4 2 7 5 5 6 4 1 0 2 3 1 3 1 2 0 1 4 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 3 1 0 0 3 4 6 33 6 5 17 3 1 2 0 4 0 2 1 1 0 1 4 3
Unique Cell Count 325 482 23 113 101 184 147 214 95 135 208 224 322 385 451 87 122 73 330 493 74 210 401 307
Labelled Cell Count 330 490 23 113 106 189 164 231 117 154 264 246 325 388 454 97 126 83 330 493 74 210 401 307


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 9.8 10.8 10.6 6.8 8.0 5.0 6.5 5.2 4.9 4.9 4.9 11.1 9.9 8.7 8.3 10.3 10.5 10.2 10.5 11.0
Std Deviation (1e-4) 1.8 1.5 1.7 1.7 1.5 1.5 1.5 1.3 1.2 0.7 1.0 0.9 1.7 1.5 1.3 1.3 1.8 2.0 2.2 2.5 2.5
Intensity Change (Log2) – – – -0.02 -0.66 -0.42 -1.12 -0.72 -1.06 -1.12 -1.12 -1.13 0.04 -0.13 -0.31 -0.39 -0.06 -0.03 -0.07 -0.04 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -1.6 -1.2 -2.0 -2.5 -2.6 -2.9 -3.3 -3.1 -0.6 -0.5 -0.2 -1.0 0 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.2 3.0 4.0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1315 6.5443 5.1755 4.9303 4.7784 5.3991 7.3418 8.1289 7.5192 7.1539 7.5941 7.9702 5.378 6.514 6.3626 6.3126 5.7792 5.8122
Actin 0.0136 0.0018 0.0194 0.047 0.0552 0.0022 0.0409 0.0007 0.0138 0.0003 0.0014 0.0036 0.0202 0.0004 0.0025 0.0004 0.0861 0.0031
Bud 0.0002 0.0003 0.0004 0.0006 0.0004 0.0001 0.0009 0.0001 0.0002 0.0001 0.0005 0.0003 0.0005 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0002 0.0002 0.0015 0.0003 0.0005 0.0009 0.0026 0.0002 0.0008 0.0002 0.0003 0.0012 0.0078 0.0002 0.0006 0.0004 0.0009 0.0009
Bud Periphery 0.0001 0.0002 0.0007 0.0008 0.0007 0.0001 0.0009 0 0.0002 0 0.0006 0.0003 0.001 0 0.0001 0 0.0001 0
Bud Site 0.0004 0.0035 0.001 0.0005 0.0005 0.0006 0.0085 0.0005 0.0029 0.0001 0.0003 0.0001 0.0096 0.0007 0.0005 0.0001 0.0004 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0 0.0002 0 0.0002 0 0.0001 0 0.0011 0.0001 0.0001 0 0 0
Cytoplasm 0.8482 0.9384 0.9133 0.8758 0.8595 0.9348 0.807 0.8267 0.9165 0.9055 0.9176 0.9008 0.8191 0.9608 0.914 0.9739 0.8054 0.9347
Cytoplasmic Foci 0.0165 0.0043 0.0161 0.003 0.0076 0.0088 0.0201 0.0028 0.0122 0.0032 0.0039 0.0082 0.0261 0.0026 0.0076 0.0022 0.0103 0.0054
Eisosomes 0.0001 0.0001 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0037 0.0034 0.0025 0.0037 0.0009 0.0018 0.01 0.0017 0.0027 0.0021 0.0037 0.0022 0.0049 0.0013 0.0019 0.0011 0.0012 0.0016
Endosome 0.0045 0.0032 0.002 0.0023 0.0055 0.0036 0.0197 0.0022 0.0033 0.001 0.0033 0.0028 0.0108 0.0004 0.0065 0.0007 0.0027 0.0036
Golgi 0.0037 0.0042 0.001 0.0008 0.015 0.0011 0.0043 0.0001 0.0016 0.0001 0.0002 0.0007 0.0052 0.0001 0.0014 0.0001 0.0037 0.0008
Lipid Particles 0.0079 0.0009 0.0008 0.0002 0.0115 0.0029 0.0026 0.0001 0.0016 0 0.0002 0.0001 0.0107 0 0.0026 0 0.0036 0.0008
Mitochondria 0.0118 0.0109 0.0008 0.0008 0.0083 0.0004 0.0016 0.0002 0.0005 0.0001 0.0009 0.0003 0.0008 0.0001 0.0003 0.0001 0.0003 0.0063
None 0.0557 0.0234 0.02 0.0579 0.0026 0.0136 0.0588 0.1523 0.0318 0.0817 0.0453 0.067 0.0354 0.0222 0.0424 0.0117 0.0653 0.034
Nuclear Periphery 0.0059 0.0008 0.0014 0.0011 0.0006 0.0016 0.0085 0.0018 0.001 0.0009 0.0066 0.0009 0.0021 0.0023 0.0013 0.0006 0.0009 0.0012
Nucleolus 0.0001 0.0001 0.0001 0 0 0.001 0.0001 0.0001 0.0001 0 0.0002 0 0.0004 0 0.0001 0 0.0001 0
Nucleus 0.0059 0.0019 0.0049 0.0038 0.0028 0.0099 0.0043 0.0082 0.0032 0.0037 0.0107 0.007 0.0041 0.008 0.0131 0.0078 0.0037 0.0046
Peroxisomes 0.0187 0.001 0.012 0.0002 0.0269 0.0016 0.003 0.0001 0.0021 0 0.0001 0.0027 0.0222 0.0001 0.001 0 0.009 0.0007
Punctate Nuclear 0.0017 0.0002 0.0014 0.0003 0.0008 0.0142 0.0031 0.0015 0.0045 0.0005 0.0014 0.0014 0.0164 0.0004 0.0035 0.0004 0.0057 0.0019
Vacuole 0.0006 0.0008 0.0004 0.0004 0.0002 0.0005 0.0018 0.0007 0.0004 0.0003 0.0018 0.0003 0.0012 0.0002 0.0003 0.0002 0.0003 0.0003
Vacuole Periphery 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.001 0.0001 0.0001 0.0001 0.0008 0.0001 0.0002 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.5678 8.6524 18.4589 12.5544 18.0395 14.5104 25.1518 23.6185 19.0709 21.6768
Translational Efficiency 1.0651 2.4042 0.8164 1.3548 0.9852 1.5139 1.1923 0.8614 1.2662 1.1494

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1313 1255 2265 1578 1998 1566 120 1623 3311 2821 2385 3201

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 989.45 1044.92 1309.70 1250.39 991.35 1244.79 1343.97 1216.00 990.60 1155.87 1311.42 1232.95
Standard Deviation 147.02 162.21 181.52 193.25 144.52 159.06 158.11 200.52 145.52 188.72 180.57 197.72
Intensity Change Log 2 — 0.078694 0.404538 0.337679 — 0.328436 0.439035 0.294677 — 0.209082 0.421906 0.316318

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000323 0.000078 0.000921 0.000107 0.000116 0.000820 0.003048 0.000089 0.000208 0.000115 0.002000
Bud Neck 0.000674 0.000630 0.000917 0.006094 0.000625 0.001111 0.003285 0.005863 0.000645 0.000897 0.001037 0.005977
Bud Site 0.002083 0.002982 0.002322 0.021228 0.001644 0.004856 0.007415 0.041816 0.001818 0.004022 0.002579 0.031667
Cell Periphery 0.000027 0.000052 0.000025 0.000088 0.000044 0.000045 0.000254 0.000145 0.000037 0.000048 0.000037 0.000117
Cytoplasm 0.812155* 0.860312* 0.660490* 0.650776* 0.831335* 0.850366* 0.468485* 0.684556* 0.823729* 0.854791* 0.650829* 0.667903*
Cytoplasmic Foci 0.032499 0.016945 0.001776 0.008948 0.041566 0.035019 0.016317 0.015061 0.037971 0.026978 0.002508 0.012048
Eisosomes 0.000005 0.000005 0.000002 0.000009 0.000008 0.000008 0.000037 0.000017 0.000007 0.000007 0.000004 0.000013
Endoplasmic Reticulum 0.000504 0.000523 0.000182 0.000507 0.000638 0.000229 0.003192 0.000800 0.000585 0.000360 0.000333 0.000656
Endosome 0.000988 0.001479 0.000123 0.003087 0.001306 0.001990 0.003644 0.009522 0.001180 0.001763 0.000300 0.006350
Golgi 0.000372 0.001341 0.000060 0.003764 0.000530 0.001283 0.000270 0.010148 0.000467 0.001309 0.000070 0.007001
Lipid Particles 0.000343 0.000307 0.000108 0.000544 0.000482 0.000299 0.002078 0.000717 0.000427 0.000303 0.000207 0.000632
Mitochondria 0.000494 0.000936 0.000245 0.002578 0.000134 0.000473 0.000300 0.002350 0.000277 0.000679 0.000247 0.002463
Mitotic Spindle 0.002862 0.001132 0.002977 0.086309 0.002029 0.004760 0.001800 0.050444 0.002359 0.003146 0.002918 0.068124
None 0.004244 0.008545 0.009742 0.007834 0.005223 0.006237 0.004667 0.008965 0.004835 0.007264 0.009486 0.008408
Nuclear Periphery 0.000146 0.000073 0.000291 0.001311 0.000116 0.000082 0.001451 0.000313 0.000128 0.000078 0.000349 0.000805
Nuclear Periphery Foci 0.001387 0.002737 0.000250 0.002172 0.001845 0.001279 0.014659 0.001916 0.001664 0.001928 0.000975 0.002043
Nucleolus 0.000307 0.000727 0.000079 0.000224 0.000146 0.000190 0.000230 0.000233 0.000210 0.000429 0.000086 0.000229
Nucleus 0.099692 0.079173 0.312438* 0.141540* 0.085069 0.062990 0.279233* 0.104616 0.090868 0.070190 0.310768* 0.122818
Peroxisomes 0.000142 0.000526 0.000083 0.000708 0.000192 0.000809 0.000170 0.002382 0.000172 0.000683 0.000088 0.001557
Vacuole 0.040655 0.021042 0.007772 0.058822 0.026708 0.027289 0.191529 0.053440 0.032239 0.024510 0.017018 0.056093
Vacuole Periphery 0.000359 0.000209 0.000041 0.002533 0.000253 0.000569 0.000164 0.003647 0.000295 0.000409 0.000047 0.003098

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.31 -3.64 -5.29 0.05 -4.45 -0.89 -7.16 -3.70 -3.60 -0.20 -1.40 -3.26 -4.85 -2.59 -4.35
Bud Neck 0.22 -4.47 -6.86 -6.19 -4.93 -2.40 -7.69 -5.16 -4.01 2.43 -1.83 -7.30 -7.82 -6.47 -5.09
Bud Site -1.61 -0.58 -5.33 -4.41 -5.12 -3.91 -7.62 -9.02 -6.09 -2.56 -4.25 -2.15 -10.62 -7.29 -8.75
Cell Periphery -6.00 -3.89 -11.53 -5.10 -9.85 -0.26 -8.85 -7.06 -6.28 7.16 -3.66 -4.12 -12.09 -8.18 -8.49
Cytoplasm -7.62 28.02 30.10 37.58 7.18 -2.65 20.68 30.40 30.75 -10.43 -7.00 40.63 43.69 48.14 2.58
Cytoplasmic Foci 6.58 16.44 13.51 8.11 -12.07 2.23 11.60 18.27 14.73 2.17 6.00 28.47 23.06 16.80 -15.35
Eisosomes -0.84 4.70 -12.77 -8.41 -19.54 -0.35 -9.87 -6.46 -6.10 7.57 -0.62 3.75 -11.55 -10.00 -14.56
Endoplasmic Reticulum -0.52 3.39 0.40 0.90 -4.30 3.76 -6.64 1.28 -3.03 7.25 2.88 1.97 1.42 -1.98 -0.71
Endosome -1.33 9.76 1.19 1.79 -5.42 -1.55 -5.06 -1.84 -0.47 2.29 -1.97 9.65 -1.26 0.59 -5.06
Golgi -1.58 4.22 -0.79 1.15 -2.08 -1.42 0.92 -2.21 -1.04 -2.66 -2.08 2.92 -2.26 -0.11 -3.58
Lipid Particles 0.21 2.94 -12.42 -11.00 -16.72 1.80 -8.29 -5.38 -8.46 6.65 1.71 1.80 -11.04 -13.80 -16.96
Mitochondria -0.59 0.64 -0.49 0.36 -1.28 -1.73 -4.03 -4.28 -1.03 -2.85 -1.18 -0.69 -2.46 -0.04 -2.49
Mitotic Spindle 1.25 -0.80 -7.97 -8.89 -7.91 -1.72 -1.89 -7.42 -5.93 -5.90 -0.78 -1.57 -10.93 -10.41 -10.08
None -5.14 -11.96 -7.56 -0.63 2.88 -1.05 0.05 -5.68 -3.15 -2.65 -4.12 -14.32 -9.25 -2.87 3.33
Nuclear Periphery 5.93 -16.87 -10.54 -11.32 -6.75 1.87 -11.02 -20.32 -22.49 7.36 4.51 -20.47 -15.91 -16.96 -6.52
Nuclear Periphery Foci -4.90 8.21 -0.94 4.36 -9.49 2.56 -6.80 0.91 -1.47 6.92 -2.23 4.99 0.45 2.61 -4.60
Nucleolus -0.90 3.83 1.36 1.06 -10.22 -0.99 -4.15 -3.33 -0.71 1.46 -1.06 4.32 -0.95 0.95 -8.06
Nucleus 5.64 -46.55 -15.26 -21.08 29.92 8.26 -8.68 -15.92 -21.92 5.62 9.35 -55.09 -22.81 -30.67 36.55
Peroxisomes -2.12 2.52 -0.78 1.85 -2.21 -2.34 -0.15 -2.34 0.56 -2.33 -3.05 3.02 -2.39 1.42 -3.42
Vacuole 5.92 10.14 -23.59 -27.54 -30.60 -2.00 -11.68 -25.05 -23.97 5.08 3.00 1.81 -35.05 -36.46 -34.33
Vacuole Periphery 1.75 4.59 -0.18 -0.90 -1.77 -0.95 0.60 -1.81 -0.60 -2.29 -0.58 3.11 -1.60 -0.83 -3.29
Standard name
Human Ortholog
Description Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM3 has a paralog, GPM2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gpm3

Gpm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available