Standard name
Human Ortholog
Description Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM3 has a paralog, GPM2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 1.0 0.98 0.9 0.94 0.84 0.79 0.76 0.73 0.67 0.69 0.98 0.99 1.0 0.95 1.0 0.96 0.96 0.95 0.98 0.91 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0 0.16 0.09 0.33 0.3 0.43 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.15 0.06 0 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 3 0 4 0 2 1 5 4 0 0 0 0 0 0 0 1 0 3 12 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 2 0 0 0 1 4 3 0 0 4 1 0 3 1 2 1 1 0 0 0 0 0 0
Cytoplasm 318 472 23 111 91 173 124 168 72 98 140 155 317 380 450 83 122 70 315 466 72 190 363 285
Endoplasmic Reticulum 1 4 0 0 1 5 0 0 0 4 0 7 4 1 1 1 1 6 0 3 0 1 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 4 0 0 1 2
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 2 0 0 0 0
Mitochondria 2 3 0 0 5 2 23 20 31 40 89 68 1 2 0 3 1 0 1 4 0 3 1 3
Nucleus 2 6 0 2 3 4 2 7 5 5 6 4 1 0 2 3 1 3 1 2 0 1 4 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 3 1 0 0 3 4 6 33 6 5 17 3 1 2 0 4 0 2 1 1 0 1 4 3
Unique Cell Count 325 482 23 113 101 184 147 214 95 135 208 224 322 385 451 87 122 73 330 493 74 210 401 307
Labelled Cell Count 330 490 23 113 106 189 164 231 117 154 264 246 325 388 454 97 126 83 330 493 74 210 401 307


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 9.8 10.8 10.6 6.8 8.0 5.0 6.5 5.2 4.9 4.9 4.9 11.1 9.9 8.7 8.3 10.3 10.5 10.2 10.5 11.0
Std Deviation (1e-4) 1.8 1.5 1.7 1.7 1.5 1.5 1.5 1.3 1.2 0.7 1.0 0.9 1.7 1.5 1.3 1.3 1.8 2.0 2.2 2.5 2.5
Intensity Change (Log2) -0.02 -0.66 -0.42 -1.12 -0.72 -1.06 -1.12 -1.12 -1.13 0.04 -0.13 -0.31 -0.39 -0.06 -0.03 -0.07 -0.04 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -1.6 -1.2 -2.0 -2.5 -2.6 -2.9 -3.3 -3.1 -0.6 -0.5 -0.2 -1.0 0 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.2 3.0 4.0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1315 6.5443 5.1755 4.9303 4.7784 5.3991 7.3418 8.1289 7.5192 7.1539 7.5941 7.9702 5.378 6.514 6.3626 6.3126 5.7792 5.8122
Actin 0.0136 0.0018 0.0194 0.047 0.0552 0.0022 0.0409 0.0007 0.0138 0.0003 0.0014 0.0036 0.0202 0.0004 0.0025 0.0004 0.0861 0.0031
Bud 0.0002 0.0003 0.0004 0.0006 0.0004 0.0001 0.0009 0.0001 0.0002 0.0001 0.0005 0.0003 0.0005 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0002 0.0002 0.0015 0.0003 0.0005 0.0009 0.0026 0.0002 0.0008 0.0002 0.0003 0.0012 0.0078 0.0002 0.0006 0.0004 0.0009 0.0009
Bud Periphery 0.0001 0.0002 0.0007 0.0008 0.0007 0.0001 0.0009 0 0.0002 0 0.0006 0.0003 0.001 0 0.0001 0 0.0001 0
Bud Site 0.0004 0.0035 0.001 0.0005 0.0005 0.0006 0.0085 0.0005 0.0029 0.0001 0.0003 0.0001 0.0096 0.0007 0.0005 0.0001 0.0004 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0 0.0002 0 0.0002 0 0.0001 0 0.0011 0.0001 0.0001 0 0 0
Cytoplasm 0.8482 0.9384 0.9133 0.8758 0.8595 0.9348 0.807 0.8267 0.9165 0.9055 0.9176 0.9008 0.8191 0.9608 0.914 0.9739 0.8054 0.9347
Cytoplasmic Foci 0.0165 0.0043 0.0161 0.003 0.0076 0.0088 0.0201 0.0028 0.0122 0.0032 0.0039 0.0082 0.0261 0.0026 0.0076 0.0022 0.0103 0.0054
Eisosomes 0.0001 0.0001 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0037 0.0034 0.0025 0.0037 0.0009 0.0018 0.01 0.0017 0.0027 0.0021 0.0037 0.0022 0.0049 0.0013 0.0019 0.0011 0.0012 0.0016
Endosome 0.0045 0.0032 0.002 0.0023 0.0055 0.0036 0.0197 0.0022 0.0033 0.001 0.0033 0.0028 0.0108 0.0004 0.0065 0.0007 0.0027 0.0036
Golgi 0.0037 0.0042 0.001 0.0008 0.015 0.0011 0.0043 0.0001 0.0016 0.0001 0.0002 0.0007 0.0052 0.0001 0.0014 0.0001 0.0037 0.0008
Lipid Particles 0.0079 0.0009 0.0008 0.0002 0.0115 0.0029 0.0026 0.0001 0.0016 0 0.0002 0.0001 0.0107 0 0.0026 0 0.0036 0.0008
Mitochondria 0.0118 0.0109 0.0008 0.0008 0.0083 0.0004 0.0016 0.0002 0.0005 0.0001 0.0009 0.0003 0.0008 0.0001 0.0003 0.0001 0.0003 0.0063
None 0.0557 0.0234 0.02 0.0579 0.0026 0.0136 0.0588 0.1523 0.0318 0.0817 0.0453 0.067 0.0354 0.0222 0.0424 0.0117 0.0653 0.034
Nuclear Periphery 0.0059 0.0008 0.0014 0.0011 0.0006 0.0016 0.0085 0.0018 0.001 0.0009 0.0066 0.0009 0.0021 0.0023 0.0013 0.0006 0.0009 0.0012
Nucleolus 0.0001 0.0001 0.0001 0 0 0.001 0.0001 0.0001 0.0001 0 0.0002 0 0.0004 0 0.0001 0 0.0001 0
Nucleus 0.0059 0.0019 0.0049 0.0038 0.0028 0.0099 0.0043 0.0082 0.0032 0.0037 0.0107 0.007 0.0041 0.008 0.0131 0.0078 0.0037 0.0046
Peroxisomes 0.0187 0.001 0.012 0.0002 0.0269 0.0016 0.003 0.0001 0.0021 0 0.0001 0.0027 0.0222 0.0001 0.001 0 0.009 0.0007
Punctate Nuclear 0.0017 0.0002 0.0014 0.0003 0.0008 0.0142 0.0031 0.0015 0.0045 0.0005 0.0014 0.0014 0.0164 0.0004 0.0035 0.0004 0.0057 0.0019
Vacuole 0.0006 0.0008 0.0004 0.0004 0.0002 0.0005 0.0018 0.0007 0.0004 0.0003 0.0018 0.0003 0.0012 0.0002 0.0003 0.0002 0.0003 0.0003
Vacuole Periphery 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.001 0.0001 0.0001 0.0001 0.0008 0.0001 0.0002 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.5678 8.6524 18.4589 12.5544 18.0395 14.5104 25.1518 23.6185 19.0709 21.6768
Translational Efficiency 1.0651 2.4042 0.8164 1.3548 0.9852 1.5139 1.1923 0.8614 1.2662 1.1494

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM3 has a paralog, GPM2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gpm3

Gpm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available