Standard name
Human Ortholog
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0.05 0 0.05 0.09 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.95 0.89 0.78 0.71 0.66 0.63 0.61 0.59 0.52 0.95 0.96 0.96 0.86 0.94 0.93 0.94 0.95 0.88 0.92 0.88 0.84
Endoplasmic Reticulum 0 0 0 0 0.06 0.05 0.09 0.15 0.12 0.07 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.24 0.24 0.2 0.35 0.39 0.4 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.09 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 4 4 7 12 11 5 13 20 0 0 1 4 0 1 0 0 1 0 1 1
Bud 0 1 2 0 1 1 0 1 3 3 1 2 0 0 0 1 0 0 0 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 6 6 7 9 15 17 14 13 23 26 38 10 9 15 6 2 0 0 0 2 0 0 0
Cytoplasm 189 360 310 194 210 248 246 225 166 155 176 167 337 507 492 220 173 185 179 355 287 227 305 250
Endoplasmic Reticulum 0 11 2 6 13 16 30 51 32 19 24 34 14 16 16 7 5 4 4 3 14 7 15 21
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1 0 0 0 3 1 1 1
Golgi 1 0 0 0 1 1 3 1 2 1 3 2 0 1 3 3 1 1 0 0 1 0 5 2
Mitochondria 2 0 5 3 9 75 82 67 93 99 120 143 0 5 2 11 0 1 0 1 1 3 2 2
Nucleus 0 0 1 0 2 1 4 2 0 2 0 1 2 0 1 1 3 3 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 6 1 4 6 9 1 6 3 5 1 1 0 34 17 12 1 4 6 4 3 7
Unique Cell Count 190 367 318 204 235 319 346 341 264 256 299 322 355 526 510 257 185 198 190 373 328 248 345 298
Labelled Cell Count 192 378 327 217 250 365 395 382 321 315 366 414 364 539 530 292 202 207 190 373 328 248 345 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.4 18.9 13.2 13.0 12.2 10.6 9.5 9.9 10.5 9.9 9.7 9.9 16.9 15.9 15.4 17.3 20.2 20.3 13.4 13.5 14.6
Std Deviation (1e-4) 2.8 3.1 2.3 2.1 2.1 1.7 1.9 1.9 1.9 2.1 1.9 2.1 2.4 2.6 2.7 4.7 4.8 5.7 2.8 2.8 2.8
Intensity Change (Log2) -0.02 -0.12 -0.31 -0.48 -0.42 -0.34 -0.41 -0.44 -0.42 0.36 0.26 0.22 0.39 0.61 0.62 0.02 0.02 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.4 0 0 3.8 4.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 1.4 2.0 2.1 1.7 2.1 3.9 3.8 5.0 0.8 -0.2 0.9 0.4 0 0
Cytoplasm -1.5 -4.0 -7.6 -9.2 -10.3 -10.8 -11.2 -11.7 -13.2 -1.7 -0.9 -0.8 -5.3 -2.2 -2.3
Endoplasmic Reticulum 0 3.5 3.3 4.8 6.8 5.9 4.3 4.6 5.5 2.8 2.4 2.4 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.7 8.4 8.4 7.4 10.8 11.5 11.9 12.9 0 0 0 2.0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 6.4 5.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.8789 16.2286 13.5481 13.4053 12.4029 13.8043 11.6397 14.8277 13.3596 13.5498 12.7828 14.0104 14.1391 17.528 15.5361 14.6863 14.63 15.6955
Actin 0.0103 0.0083 0.016 0.0009 0.0014 0.017 0.0041 0.0105 0.0195 0.002 0.0021 0.0026 0.0122 0.0009 0.0009 0.0061 0.0122 0.0007
Bud 0.001 0.0004 0.0014 0.0011 0.0012 0.0005 0.0015 0.0004 0.0009 0.0003 0.0023 0.0003 0.0004 0.0002 0.0005 0.0006 0.0004 0.0002
Bud Neck 0.001 0.0005 0.004 0.0011 0.0013 0.0019 0.0013 0.0004 0.0008 0.0004 0.001 0.0013 0.0006 0.0003 0.0007 0.0021 0.0007 0.001
Bud Periphery 0.0008 0.0004 0.0019 0.0005 0.0013 0.0005 0.0015 0.0001 0.0008 0.0003 0.0039 0.0002 0.0004 0 0.0002 0.0003 0.0003 0.0001
Bud Site 0.0033 0.0033 0.0102 0.0006 0.0045 0.0003 0.0014 0.0022 0.0022 0.0006 0.0029 0.0001 0.0012 0.0009 0.0013 0.0215 0.0007 0.0001
Cell Periphery 0.001 0.0007 0.0014 0.0002 0.0006 0.0004 0.0008 0.0002 0.0003 0.0002 0.0004 0.0001 0.0007 0.0002 0.0004 0.0002 0.0001 0.0002
Cytoplasm 0.7663 0.9392 0.7856 0.8948 0.7552 0.8368 0.7941 0.9405 0.843 0.7846 0.7883 0.8921 0.8135 0.9628 0.9 0.881 0.798 0.909
Cytoplasmic Foci 0.0132 0.0038 0.0118 0.0126 0.0227 0.0133 0.0187 0.0081 0.0205 0.015 0.0156 0.015 0.0177 0.0146 0.0112 0.01 0.0233 0.0107
Eisosomes 0.0002 0.0001 0.0002 0 0.0001 0.0002 0 0.0001 0.0001 0 0.0001 0 0.0002 0 0 0.0001 0.0003 0
Endoplasmic Reticulum 0.0614 0.0216 0.0345 0.0032 0.0486 0.0447 0.0465 0.0075 0.0161 0.0494 0.013 0.029 0.0628 0.0075 0.0213 0.0109 0.0205 0.0324
Endosome 0.0527 0.007 0.0484 0.033 0.0533 0.043 0.0555 0.0108 0.0475 0.083 0.0901 0.0315 0.0406 0.0063 0.0313 0.0363 0.0869 0.0291
Golgi 0.0113 0.002 0.0142 0.0111 0.0057 0.0109 0.0163 0.0017 0.0132 0.0076 0.0085 0.0091 0.0115 0.0014 0.0052 0.0014 0.0079 0.0029
Lipid Particles 0.0082 0.0008 0.0043 0 0.0045 0.0031 0.0044 0.0002 0.005 0.0017 0.0021 0.0031 0.0038 0.0003 0.0009 0.0007 0.0053 0.0002
Mitochondria 0.0235 0.0023 0.0229 0.0132 0.0077 0.0118 0.0196 0.0005 0.0076 0.0294 0.0325 0.0038 0.0032 0.0002 0.0018 0.0005 0.0152 0.0038
None 0.0022 0.0004 0.0022 0.0006 0.0015 0.0005 0.0015 0.0007 0.0007 0.0005 0.0013 0.0006 0.0048 0.0003 0.0008 0.0006 0.006 0.0009
Nuclear Periphery 0.0108 0.0012 0.0108 0.0021 0.0104 0.0017 0.0074 0.0006 0.0018 0.002 0.0049 0.0043 0.009 0.001 0.0023 0.0022 0.0057 0.0017
Nucleolus 0.0007 0.0001 0.0003 0 0.0021 0 0.0001 0.0088 0.0002 0.0001 0.0004 0 0.0002 0 0 0.0002 0.0003 0.0001
Nucleus 0.0037 0.0012 0.003 0.0026 0.022 0.0014 0.006 0.0028 0.0011 0.0011 0.0042 0.0022 0.0029 0.0008 0.0018 0.002 0.0028 0.0017
Peroxisomes 0.004 0.0007 0.0021 0.0001 0.0051 0.0007 0.0007 0.0001 0.0058 0.0006 0.0008 0.0007 0.0022 0.0002 0.0002 0.0009 0.0022 0.0001
Punctate Nuclear 0.0034 0.0001 0.0016 0.0001 0.0019 0.0003 0.0004 0.0002 0.0007 0.0001 0.0005 0.0002 0.0027 0.0001 0.0002 0.0009 0.0015 0.0008
Vacuole 0.0171 0.0055 0.0177 0.0186 0.0388 0.0079 0.0149 0.003 0.009 0.017 0.018 0.0027 0.0078 0.0017 0.0155 0.0189 0.0063 0.0029
Vacuole Periphery 0.0039 0.0007 0.0051 0.0035 0.0101 0.0031 0.0029 0.0005 0.0033 0.004 0.0071 0.0011 0.0015 0.0003 0.0033 0.0029 0.0033 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.105 96.5868 93.2712 80.1161 84.9156 68.0463 70.7358 74.0394 68.1052 80.3019
Translational Efficiency 1.5423 1.3081 1.2441 1.1858 1.2097 1.5894 1.3756 1.1083 1.1183 1.1258

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Prs5

Prs5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prs5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available