Standard name
Human Ortholog
Description Bisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway; human homolog BPNT1 complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.99 0.99 0.99 0.95 0.94 0.85 0.74 0.79 0.77 0.79 0.77 0.95 0.98 0.98 0.98 0.98 0.99 0.9 0.94 0.97 0.94 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0 0 0.09 0.06 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.14 0.07 0.1 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 5 1 3 3 6 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 3 1 3 1 0 1 5 2 0 0 2 1 0 0 1 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 3 13 12 6 13 10 15 0 2 2 8 3 6 0 0 0 0 0 0
Cytoplasm 15 133 129 261 428 308 458 436 309 325 254 400 156 298 307 415 340 356 53 128 128 186 300 282
Endoplasmic Reticulum 0 0 0 1 1 4 34 30 18 16 9 19 1 4 4 17 8 15 0 1 0 1 4 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 4 8 17 19 37 27 37 67 1 0 0 3 0 0 0 1 0 0 0 0
Nucleus 0 0 1 1 4 3 1 13 6 13 7 8 0 1 3 0 0 0 1 1 0 3 7 7
Nuclear Periphery 0 0 0 0 0 0 0 2 3 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 2 17 9 23 84 28 44 16 42 6 2 2 12 6 5 1 0 0 2 9 8
Unique Cell Count 16 134 130 264 451 329 538 587 391 420 321 519 164 304 312 425 348 361 60 137 132 198 326 310
Labelled Cell Count 16 135 131 265 457 338 548 605 409 445 337 562 167 307 318 459 359 382 60 137 132 198 326 310


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.7 39.1 26.3 25.2 21.1 16.3 14.5 13.8 12.9 12.3 11.6 11.1 26.1 27.1 26.4 31.5 32.6 32.5 31.4 31.9 32.7
Std Deviation (1e-4) 9.9 11.3 7.8 7.5 6.0 4.3 3.5 3.4 3.2 3.6 2.9 3.3 7.9 7.8 7.9 11.4 12.7 12.2 8.8 8.9 10.1
Intensity Change (Log2) – – – -0.06 -0.32 -0.69 -0.86 -0.94 -1.03 -1.1 -1.18 -1.24 -0.01 0.04 0.0 0.26 0.31 0.3 0.25 0.28 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -2.2 -2.5 -4.4 -6.3 -5.5 -5.7 -5.4 -5.8 -2.0 -0.9 -0.7 -1.1 -1.1 -0.5
Endoplasmic Reticulum 0 0 0 2.9 2.6 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 3.0 4.0 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.3 2.8 3.5 0 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.2952 45.2525 40.5184 33.5135 35.7897 42.6957 27.9418 38.406 32.0754 31.4732 30.6432 35.7312 28.5799 38.8062 34.2147 31.6471 36.4872 33.5384
Actin 0.0002 0.0002 0.0001 0.0005 0 0.0001 0.0319 0.0001 0.013 0.0001 0.0002 0.0004 0.0202 0.0001 0.025 0.0001 0 0.0018
Bud 0.0001 0.0001 0.0001 0.0003 0.0001 0 0.0006 0 0.0006 0.0001 0.0001 0.0001 0.0007 0 0.0011 0 0 0.0001
Bud Neck 0.0002 0.0002 0.0002 0.0004 0.0003 0.0007 0.0009 0.0001 0.0019 0.0002 0.0003 0.0006 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003
Bud Periphery 0.0001 0 0 0.0001 0 0 0.0007 0 0.0004 0 0 0 0.0004 0 0.0009 0 0 0.0001
Bud Site 0.0001 0.0001 0 0.0001 0 0 0.0011 0 0.0013 0 0 0.0001 0.0007 0 0.0018 0 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0005 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.9593 0.9886 0.9815 0.9644 0.9809 0.9742 0.8395 0.9943 0.9386 0.9878 0.9909 0.9564 0.8622 0.997 0.9338 0.9965 0.9989 0.8956
Cytoplasmic Foci 0.0004 0.0003 0.0003 0.0011 0.0006 0.0003 0.0173 0.0001 0.0047 0.0006 0.0008 0.0105 0.0035 0.0001 0.001 0.0002 0 0.0005
Eisosomes 0 0 0 0 0 0 0.0006 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0013 0.0004 0.0004 0.0008 0.0007 0.0013 0.008 0.0001 0.0014 0.0002 0.0002 0.0022 0.0198 0 0.0002 0 0 0.003
Endosome 0.0005 0.0001 0.0001 0.0004 0.0002 0.0001 0.0152 0 0.0079 0.0001 0.0003 0.0073 0.0277 0 0.0016 0 0 0.0094
Golgi 0 0 0 0 0 0 0.0062 0 0.0072 0 0 0.0008 0.0103 0 0.0048 0 0 0.0007
Lipid Particles 0 0 0 0 0 0 0.0079 0 0.0032 0 0 0.0015 0.0011 0 0.0001 0 0 0.0006
Mitochondria 0.0002 0.0001 0 0.0002 0 0 0.0032 0 0.004 0.0002 0 0.0006 0.0063 0 0.0254 0 0 0.0027
None 0.0006 0.0003 0.0003 0.0009 0.0004 0.0002 0.0107 0.0002 0.0073 0.0004 0.0002 0.0011 0.0023 0.0001 0.0002 0.0001 0 0.0005
Nuclear Periphery 0.006 0.0015 0.0022 0.0041 0.0029 0.0031 0.0182 0.0004 0.001 0.001 0.0011 0.005 0.023 0.0002 0.0003 0.0002 0 0.0605
Nucleolus 0.0003 0 0 0.0001 0 0 0.0003 0 0.0001 0 0 0.0003 0.0001 0 0 0 0 0.0004
Nucleus 0.0278 0.0076 0.013 0.0232 0.0127 0.0194 0.0217 0.0046 0.0042 0.0088 0.0057 0.0096 0.0167 0.0022 0.003 0.0026 0.0008 0.0176
Peroxisomes 0 0 0 0.0001 0 0 0.0037 0 0.0011 0 0 0.0002 0.0003 0 0.0002 0 0 0.0001
Punctate Nuclear 0.0004 0.0003 0.0007 0.0029 0.0002 0.0002 0.0091 0 0.0005 0.0002 0.0001 0.0008 0.0007 0 0.0001 0 0 0.0012
Vacuole 0.0019 0.0003 0.0007 0.0004 0.0008 0.0003 0.0018 0 0.0011 0.0002 0.0001 0.0013 0.0014 0 0.0001 0 0 0.0015
Vacuole Periphery 0.0004 0 0.0001 0.0001 0.0001 0.0001 0.001 0 0.0003 0.0001 0.0001 0.001 0.0021 0 0.0001 0 0 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.2112 52.3496 51.1127 58.5188 55.2797 64.689 66.6276 60.4811 66.4129 70.1874
Translational Efficiency 1.7362 1.6171 1.6082 1.5881 1.7337 2.1489 1.7154 1.5809 1.6657 1.7429

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1050 1634 439 1364 1304 1074 1279 840 2354 2708 1718 2204

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2428.67 2661.47 2283.19 1568.85 2695.59 2313.31 1948.34 1641.08 2576.53 2523.39 2033.90 1596.38
Standard Deviation 617.85 925.36 460.13 334.26 677.39 735.29 390.94 335.24 664.88 871.85 434.98 336.46
Intensity Change Log 2 — 0.132057 -0.089116 -0.630459 — -0.220642 -0.468356 -0.715956 — -0.042711 -0.276164 -0.674802

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000232 0.000105 0.000284 0.005152 0.000195 0.000324 0.000250 0.001321 0.000211 0.000192 0.000258 0.003692
Bud Neck 0.000646 0.000469 0.002343 0.004317 0.000849 0.000882 0.000832 0.002138 0.000758 0.000633 0.001218 0.003486
Bud Site 0.002831 0.001751 0.002740 0.017585 0.001557 0.003624 0.001899 0.030056 0.002125 0.002493 0.002114 0.022338
Cell Periphery 0.000385 0.000171 0.000589 0.000255 0.000385 0.000392 0.000117 0.000161 0.000385 0.000258 0.000237 0.000219
Cytoplasm 0.787952* 0.798501* 0.551691* 0.680482* 0.763385* 0.812901* 0.712327* 0.733773* 0.774343* 0.804212* 0.671280* 0.700792*
Cytoplasmic Foci 0.008206 0.004254 0.015183 0.015648 0.008555 0.015987 0.006771 0.020093 0.008399 0.008907 0.008920 0.017342
Eisosomes 0.000001 0.000001 0.000003 0.000010 0.000001 0.000002 0.000002 0.000010 0.000001 0.000001 0.000002 0.000010
Endoplasmic Reticulum 0.000094 0.000057 0.000130 0.000761 0.000149 0.000278 0.000069 0.000554 0.000124 0.000144 0.000084 0.000682
Endosome 0.000105 0.000047 0.000238 0.010045 0.000279 0.000427 0.000376 0.011988 0.000201 0.000198 0.000341 0.010786
Golgi 0.000891 0.000362 0.000399 0.004646 0.001653 0.001196 0.000773 0.007897 0.001313 0.000692 0.000678 0.005885
Lipid Particles 0.000464 0.000176 0.001241 0.002280 0.000750 0.001046 0.000364 0.001295 0.000623 0.000521 0.000588 0.001905
Mitochondria 0.000114 0.000167 0.000340 0.000662 0.000182 0.000512 0.000175 0.001717 0.000152 0.000304 0.000217 0.001064
Mitotic Spindle 0.001702 0.000777 0.006883 0.050627 0.002629 0.003440 0.003022 0.023033 0.002215 0.001833 0.004009 0.040111
None 0.012271 0.015973 0.010803 0.009089 0.009192 0.007876 0.014404 0.009804 0.010565 0.012761 0.013484 0.009361
Nuclear Periphery 0.000072 0.000044 0.000410 0.000522 0.000142 0.000129 0.000144 0.000212 0.000111 0.000078 0.000212 0.000404
Nuclear Periphery Foci 0.002136 0.000542 0.001858 0.004462 0.002565 0.002626 0.000746 0.002762 0.002373 0.001369 0.001031 0.003814
Nucleolus 0.000068 0.000098 0.000155 0.000226 0.000099 0.000491 0.000094 0.000077 0.000085 0.000254 0.000109 0.000170
Nucleus 0.175919* 0.173072* 0.390453* 0.134029* 0.200685* 0.141843* 0.249488* 0.087980 0.189638* 0.160686* 0.285509* 0.116479
Peroxisomes 0.000015 0.000015 0.000028 0.002000 0.000022 0.000093 0.000216 0.001087 0.000019 0.000046 0.000168 0.001652
Vacuole 0.004756 0.003106 0.014130 0.053510 0.005869 0.005551 0.007869 0.054455 0.005373 0.004076 0.009469 0.053870
Vacuole Periphery 0.001140 0.000315 0.000101 0.003692 0.000858 0.000380 0.000063 0.009585 0.000984 0.000341 0.000073 0.005938

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.15 -4.59 -1.62 -1.86 -1.11 -2.71 -1.03 -1.64 -0.88 -1.30 0.42 -2.38 -1.96 -2.00 -1.63
Bud Neck 2.51 -10.06 -8.98 -10.16 5.69 -0.07 -2.47 -3.14 -2.98 -0.24 2.30 -8.92 -8.96 -10.34 1.47
Bud Site 1.11 -3.92 -5.77 -8.62 -2.53 -4.00 -4.43 -6.35 -4.30 -5.03 -1.04 -4.24 -9.31 -9.13 -7.14
Cell Periphery 5.11 -6.07 4.65 -0.64 9.16 -0.67 4.75 6.57 8.17 4.00 3.51 0.39 7.51 5.53 8.20
Cytoplasm -1.43 25.04 21.39 24.28 -9.42 -6.26 11.15 7.80 12.58 -0.93 -5.93 22.47 21.00 26.70 -1.53
Cytoplasmic Foci 5.71 -7.74 -3.51 -9.78 6.33 -6.01 -1.88 -4.51 1.12 -2.65 -0.44 -6.14 -5.50 -4.86 1.89
Eisosomes 3.49 -10.14 -15.99 -17.32 -3.18 -2.77 -6.70 -12.67 -10.54 -9.54 0.04 -11.86 -20.23 -20.12 -11.91
Endoplasmic Reticulum 2.32 -5.51 -5.41 -6.24 -2.37 -1.28 1.43 -1.08 0.65 -2.34 -0.34 -0.58 -4.70 -3.38 -4.66
Endosome 3.96 -7.84 -3.90 -4.03 -2.75 -0.81 0.16 -2.62 -2.37 -2.63 0.33 -1.92 -4.60 -4.62 -4.10
Golgi 4.41 1.81 0.13 -1.55 -0.58 1.21 3.68 1.08 0.30 -1.53 3.22 3.98 1.22 -0.90 -1.70
Lipid Particles 2.31 -8.52 -7.01 -8.04 3.71 -1.69 -1.73 -2.99 -1.27 -2.00 0.37 -7.36 -6.88 -6.66 -0.73
Mitochondria -1.51 -8.78 -4.57 -2.25 3.38 -1.07 -0.18 -0.67 0.59 -0.64 -1.24 -5.64 -2.06 0.01 -0.35
Mitotic Spindle 0.77 -3.23 -7.69 -8.31 -2.17 -0.34 -1.45 -4.41 -3.99 -3.62 0.73 -3.69 -8.71 -9.16 -5.65
None -2.44 2.44 4.21 9.50 3.35 1.32 -3.47 4.07 1.83 7.58 -2.41 -0.67 5.85 8.81 9.26
Nuclear Periphery 5.04 -13.76 -20.36 -21.69 5.68 0.94 -8.52 -10.63 -9.03 -3.64 3.90 -16.16 -21.73 -22.68 -1.40
Nuclear Periphery Foci 4.23 -2.23 -0.92 -4.63 1.63 -0.19 3.84 0.65 0.70 -3.99 2.76 2.30 -0.04 -2.90 -2.48
Nucleolus -1.79 -11.02 -10.04 -4.03 3.37 -1.28 -0.90 0.06 1.28 1.34 -1.41 -6.37 -6.82 0.74 -0.30
Nucleus 0.15 -15.30 5.36 5.86 18.84 8.79 -7.02 19.21 10.07 27.37 6.21 -14.87 15.63 10.13 28.90
Peroxisomes 0.57 -8.09 -2.82 -2.83 -2.42 -1.57 -1.75 -2.60 -1.67 -0.89 -1.37 -2.06 -3.52 -3.23 -2.10
Vacuole 2.08 -11.28 -29.12 -29.40 -10.97 -0.34 -11.64 -22.29 -22.23 -17.45 1.82 -16.24 -36.56 -36.84 -24.35
Vacuole Periphery 0.87 1.03 0.41 -1.21 -1.77 0.95 1.63 -0.56 -1.37 -1.87 1.27 1.82 0.03 -1.82 -2.70
Standard name
Human Ortholog
Description Bisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway; human homolog BPNT1 complements yeast null mutant
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Met22

Met22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available