Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 46 | 21 | 3 | 18 | 17 | 13 | 15 | 6 | 4 | 3 | 4 | 29 | 35 | 16 | 13 | 6 | 1 | 15 | 26 | 13 | 18 | 47 | 31 |
Bud | 11 | 6 | 7 | 3 | 12 | 25 | 21 | 28 | 26 | 15 | 25 | 13 | 7 | 14 | 5 | 4 | 1 | 3 | 14 | 42 | 14 | 17 | 14 | 7 |
Bud Neck | 2 | 1 | 2 | 0 | 4 | 6 | 3 | 3 | 2 | 6 | 5 | 4 | 0 | 0 | 0 | 1 | 1 | 1 | 27 | 44 | 25 | 23 | 37 | 24 |
Bud Site | 10 | 18 | 7 | 2 | 8 | 24 | 9 | 9 | 6 | 9 | 7 | 9 | 4 | 1 | 1 | 6 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 13 | 4 | 0 | 1 | 2 | 6 | 16 | 5 | 6 | 11 | 10 | 5 | 18 | 21 | 6 | 3 | 2 | 5 | 2 | 2 | 0 | 3 | 3 |
Cytoplasm | 97 | 133 | 90 | 66 | 115 | 87 | 178 | 233 | 120 | 199 | 105 | 118 | 159 | 357 | 435 | 83 | 28 | 37 | 76 | 98 | 64 | 78 | 202 | 218 |
Endoplasmic Reticulum | 3 | 11 | 5 | 3 | 12 | 16 | 13 | 11 | 12 | 13 | 3 | 8 | 9 | 22 | 21 | 7 | 4 | 3 | 3 | 4 | 5 | 4 | 28 | 39 |
Endosome | 6 | 2 | 3 | 5 | 14 | 16 | 12 | 11 | 2 | 7 | 3 | 2 | 1 | 2 | 0 | 3 | 0 | 3 | 3 | 8 | 5 | 4 | 6 | 6 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 3 | 2 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 14 | 10 | 6 | 11 | 8 | 39 | 82 | 142 | 123 | 202 | 149 | 165 | 3 | 1 | 2 | 5 | 2 | 4 | 2 | 2 | 2 | 0 | 5 | 6 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 2 | 2 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 2 | 1 | 1 | 1 | 7 | 8 | 8 | 12 | 6 | 12 | 8 | 9 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 6 | 9 | 7 | 4 | 12 | 13 | 11 | 8 | 7 | 9 | 8 | 6 | 9 | 0 | 2 | 8 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 1 | 1 | 6 | 5 | 10 | 10 | 8 | 3 | 6 | 3 | 4 | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 |
Vac/Vac Membrane | 0 | 2 | 1 | 2 | 6 | 5 | 16 | 26 | 10 | 18 | 14 | 12 | 1 | 0 | 0 | 8 | 3 | 5 | 0 | 4 | 3 | 1 | 4 | 15 |
Unique Cell Count | 139 | 202 | 129 | 90 | 189 | 202 | 302 | 416 | 244 | 385 | 243 | 278 | 201 | 417 | 473 | 125 | 45 | 58 | 150 | 240 | 142 | 155 | 357 | 362 |
Labelled Cell Count | 176 | 253 | 156 | 106 | 224 | 268 | 382 | 526 | 331 | 512 | 344 | 366 | 228 | 452 | 508 | 155 | 54 | 65 | 150 | 240 | 142 | 155 | 357 | 362 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 8.4 | 7.3 | 7.0 | 7.6 | 6.7 | 6.1 | 6.1 | 5.4 | 5.1 | 4.9 | 4.8 | 8.6 | 8.0 | 7.9 | 12.5 | 13.6 | 15.0 | 8.4 | 8.4 | 8.6 |
Std Deviation (1e-4) | 1.1 | 1.3 | 1.1 | 1.2 | 1.4 | 1.2 | 1.4 | 1.1 | 1.2 | 1.0 | 0.9 | 0.9 | 1.2 | 1.2 | 1.1 | 3.6 | 3.6 | 4.4 | 1.2 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.05 | -0.13 | -0.26 | -0.27 | -0.44 | -0.52 | -0.57 | -0.62 | 0.23 | 0.13 | 0.11 | 0.77 | 0.89 | 1.03 | 0.19 | 0.19 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.0 | -1.8 | -2.2 | -4.2 | -5.1 | -4.9 | -7.0 | -5.6 | 0 | -0.5 | -2.6 | -5.4 | -1.4 | -0.5 | -2.9 |
Bud | 0 | 0.3 | 2.1 | 0.6 | 0.5 | 1.7 | -0.7 | 1.6 | 0 | -0.9 | 0 | 0 | -0.9 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | -0.5 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 0 | 0.6 | 0.7 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -1.6 | -4.7 | -2.1 | -2.8 | -3.8 | -3.6 | -4.9 | -5.1 | 1.9 | 4.1 | 6.7 | -0.6 | -0.9 | -0.8 |
Endoplasmic Reticulum | 0 | 1.0 | 1.5 | 0.2 | 0 | 0.5 | 0 | 0 | 0 | 0.3 | 0.6 | 0.3 | 0.6 | 0 | 0 |
Endosome | 0 | 2.0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.1 | -0.2 | 3.8 | 5.3 | 6.6 | 8.8 | 9.6 | 10.6 | 0 | 0 | 0 | 0 | -0.3 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0.3 | 0.4 | -0.8 | 0 | 0 | 0 | -1.0 | 0 | -0.4 | 0 | 0 | 0.3 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.2 | 2.5 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4788 | 4.0904 | 3.7638 | 3.3049 | 3.2665 | 4.7442 | 2.4347 | 4.2166 | 3.1658 | 3.0801 | 2.642 | 4.0792 | 3.0786 | 4.1529 | 3.8626 | 3.3773 | 3.7939 | 5.2617 |
Actin | 0.135 | 0.0133 | 0.0195 | 0.0109 | 0.0942 | 0.0319 | 0.0979 | 0.0379 | 0.0308 | 0.018 | 0.055 | 0.0303 | 0.0012 | 0.004 | 0.0659 | 0.1547 | 0.0058 | 0.0069 |
Bud | 0.0029 | 0.0021 | 0.0031 | 0.001 | 0.0044 | 0.0011 | 0.0019 | 0.0048 | 0.0046 | 0.0012 | 0.001 | 0.0013 | 0.0004 | 0.0003 | 0.0025 | 0.0014 | 0.0018 | 0.0013 |
Bud Neck | 0.0489 | 0.0063 | 0.0622 | 0.013 | 0.043 | 0.66 | 0.0613 | 0.0059 | 0.0534 | 0.0809 | 0.002 | 0.6314 | 0.2352 | 0.0017 | 0.0611 | 0.1952 | 0.2308 | 0.692 |
Bud Periphery | 0.0047 | 0.0015 | 0.006 | 0.0013 | 0.0102 | 0.0019 | 0.0019 | 0.0027 | 0.0039 | 0.0017 | 0.0019 | 0.0021 | 0.0002 | 0.0001 | 0.0025 | 0.0014 | 0.0016 | 0.0012 |
Bud Site | 0.177 | 0.2977 | 0.1306 | 0.009 | 0.0052 | 0.0064 | 0.1961 | 0.2347 | 0.0318 | 0.0014 | 0.0022 | 0.0035 | 0.0989 | 0.3429 | 0.0649 | 0.118 | 0.01 | 0.0062 |
Cell Periphery | 0.0158 | 0.0038 | 0.0041 | 0.0118 | 0.0007 | 0.0043 | 0.0054 | 0.003 | 0.0022 | 0.0004 | 0.0003 | 0.0009 | 0.0015 | 0.0007 | 0.0033 | 0.0008 | 0.0021 | 0.0009 |
Cytoplasm | 0.3869 | 0.5928 | 0.6634 | 0.7101 | 0.7699 | 0.2474 | 0.4451 | 0.6182 | 0.7137 | 0.6894 | 0.7616 | 0.3151 | 0.652 | 0.6069 | 0.6925 | 0.3311 | 0.7218 | 0.2546 |
Cytoplasmic Foci | 0.091 | 0.0076 | 0.0203 | 0.0451 | 0.0091 | 0.0194 | 0.0584 | 0.0187 | 0.0198 | 0.0211 | 0.0099 | 0.0067 | 0.0016 | 0.0212 | 0.0151 | 0.012 | 0.0136 | 0.0048 |
Eisosomes | 0.0013 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.001 | 0 | 0 | 0 | 0.0003 | 0.0012 | 0 | 0 |
Endoplasmic Reticulum | 0.0231 | 0.0027 | 0.0032 | 0.0059 | 0.0072 | 0.0025 | 0.0057 | 0.0009 | 0.0059 | 0.0029 | 0.0142 | 0.0029 | 0.0019 | 0.0038 | 0.0058 | 0.0013 | 0.0012 | 0.0042 |
Endosome | 0.0344 | 0.0009 | 0.0099 | 0.014 | 0.0076 | 0.0027 | 0.0228 | 0.0118 | 0.023 | 0.0124 | 0.0217 | 0.0015 | 0.0008 | 0.0054 | 0.0138 | 0.0086 | 0.0038 | 0.0017 |
Golgi | 0.0142 | 0.001 | 0.0062 | 0.0084 | 0.0064 | 0.0026 | 0.0182 | 0.0136 | 0.0055 | 0.0063 | 0.0128 | 0.0013 | 0.0002 | 0.0022 | 0.0311 | 0.0337 | 0.0026 | 0.001 |
Lipid Particles | 0.015 | 0.0005 | 0.0022 | 0.0168 | 0.0019 | 0.0008 | 0.0111 | 0.0002 | 0.0027 | 0.0016 | 0.0098 | 0.0001 | 0.0001 | 0.0009 | 0.0033 | 0.0069 | 0.0001 | 0 |
Mitochondria | 0.0032 | 0.0001 | 0.0012 | 0.002 | 0.0101 | 0.0003 | 0.0094 | 0.0296 | 0.0272 | 0.0521 | 0.0907 | 0.0001 | 0 | 0.0001 | 0.0142 | 0.1195 | 0.0003 | 0.0001 |
None | 0.0192 | 0.0663 | 0.0607 | 0.1155 | 0.0221 | 0.0127 | 0.0279 | 0.0141 | 0.0557 | 0.0639 | 0.0023 | 0.0009 | 0.0032 | 0.0065 | 0.0055 | 0.0028 | 0.0018 | 0.024 |
Nuclear Periphery | 0.0068 | 0.0004 | 0.0005 | 0.0008 | 0.0014 | 0.0001 | 0.0006 | 0.0002 | 0.0011 | 0.0004 | 0.0026 | 0.0001 | 0.0003 | 0.0007 | 0.0033 | 0.0002 | 0.0001 | 0.0001 |
Nucleolus | 0.0008 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0033 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0013 | 0.0008 | 0.0007 | 0.0015 | 0.0008 | 0.0004 | 0.0015 | 0.0005 | 0.0009 | 0.0005 | 0.0007 | 0.0003 | 0.0006 | 0.0006 | 0.0031 | 0.0002 | 0.0005 | 0.0002 |
Peroxisomes | 0.0053 | 0.0001 | 0.0031 | 0.02 | 0.0028 | 0.0036 | 0.0247 | 0.0013 | 0.0106 | 0.0431 | 0.0051 | 0.0006 | 0 | 0.0002 | 0.0059 | 0.0097 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0066 | 0.0004 | 0.0006 | 0.0022 | 0.0005 | 0.0004 | 0.0029 | 0.0003 | 0.0006 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.001 | 0.0025 | 0.0003 | 0.0001 | 0.0001 |
Vacuole | 0.0053 | 0.0011 | 0.0018 | 0.0092 | 0.0013 | 0.0011 | 0.003 | 0.0012 | 0.0054 | 0.0018 | 0.0025 | 0.0004 | 0.0017 | 0.0007 | 0.0021 | 0.0005 | 0.0013 | 0.0004 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0002 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0008 | 0.0006 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0006 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.7921 | 25.5436 | 50.0592 | 64.0798 | 36.0978 | 16.1099 | 45.0319 | 65.7036 | 66.2604 | 33.8108 |
Translational Efficiency | 3.281 | 3.1163 | 2.3111 | 1.8049 | 1.5676 | 1.6336 | 1.854 | 1.6652 | 1.6327 | 1.7753 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
380 | 861 | 263 | 900 | 1983 | 669 | 1649 | 1911 | 2363 | 1530 | 1912 | 2811 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 848.69 | 888.14 | 1318.83 | 1314.53 | 917.06 | 955.14 | 1189.97 | 1186.51 | 906.07 | 917.44 | 1207.69 | 1227.50 |
Standard Deviation | 96.11 | 196.21 | 136.34 | 199.43 | 112.62 | 129.03 | 143.74 | 207.24 | 112.96 | 173.35 | 149.48 | 213.31 |
Intensity Change Log 2 | — | 0.065549 | 0.635949 | 0.631237 | — | 0.058696 | 0.375837 | 0.371636 | — | 0.061994 | 0.506716 | 0.502247 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001229 | 0.002924 | 0.020905 | 0.007784 | 0.003308 | 0.004456 | 0.003945 | 0.002052 | 0.002974 | 0.003594 | 0.006278 | 0.003887 |
Bud Neck | 0.150029 | 0.048301 | 0.098263 | 0.047765 | 0.125563 | 0.098325 | 0.071808 | 0.088039 | 0.129497 | 0.070175 | 0.075447 | 0.075145 |
Bud Site | 0.315555* | 0.332931* | 0.626537* | 0.567444* | 0.379444* | 0.341484* | 0.584318* | 0.407578* | 0.369170* | 0.336671* | 0.590126* | 0.458762* |
Cell Periphery | 0.001504 | 0.000637 | 0.001545 | 0.001786 | 0.001328 | 0.000784 | 0.000870 | 0.001105 | 0.001356 | 0.000701 | 0.000963 | 0.001323 |
Cytoplasm | 0.335162* | 0.403579* | 0.121994* | 0.322408* | 0.342755* | 0.346322* | 0.289410* | 0.344109* | 0.341534* | 0.378543* | 0.266381* | 0.337161* |
Cytoplasmic Foci | 0.127366 | 0.108398 | 0.057323 | 0.015568 | 0.089141 | 0.096558 | 0.020735 | 0.025074 | 0.095288 | 0.103221 | 0.025768 | 0.022031 |
Eisosomes | 0.000362 | 0.000090 | 0.000186 | 0.000124 | 0.000267 | 0.000186 | 0.000045 | 0.000077 | 0.000282 | 0.000132 | 0.000064 | 0.000092 |
Endoplasmic Reticulum | 0.001075 | 0.001992 | 0.000617 | 0.000325 | 0.002325 | 0.000965 | 0.000703 | 0.000666 | 0.002124 | 0.001543 | 0.000691 | 0.000557 |
Endosome | 0.015827 | 0.026451 | 0.005795 | 0.002741 | 0.011329 | 0.021955 | 0.002400 | 0.005902 | 0.012052 | 0.024485 | 0.002867 | 0.004890 |
Golgi | 0.008333 | 0.009340 | 0.000929 | 0.001878 | 0.009638 | 0.009159 | 0.001328 | 0.000682 | 0.009428 | 0.009261 | 0.001273 | 0.001065 |
Lipid Particles | 0.001844 | 0.001794 | 0.002732 | 0.001298 | 0.001189 | 0.002586 | 0.000415 | 0.000670 | 0.001295 | 0.002140 | 0.000734 | 0.000871 |
Mitochondria | 0.001943 | 0.001917 | 0.000036 | 0.001391 | 0.001441 | 0.001195 | 0.000388 | 0.000215 | 0.001521 | 0.001601 | 0.000340 | 0.000591 |
Mitotic Spindle | 0.000282 | 0.001656 | 0.002582 | 0.004100 | 0.000907 | 0.001854 | 0.000726 | 0.001851 | 0.000806 | 0.001742 | 0.000981 | 0.002571 |
None | 0.000266 | 0.002120 | 0.000034 | 0.000078 | 0.000209 | 0.000170 | 0.000098 | 0.001558 | 0.000218 | 0.001268 | 0.000089 | 0.001084 |
Nuclear Periphery | 0.000018 | 0.000087 | 0.000054 | 0.000038 | 0.000017 | 0.000026 | 0.000012 | 0.000030 | 0.000017 | 0.000060 | 0.000018 | 0.000033 |
Nuclear Periphery Foci | 0.000687 | 0.000596 | 0.003029 | 0.000359 | 0.000266 | 0.000336 | 0.000163 | 0.000286 | 0.000334 | 0.000482 | 0.000557 | 0.000309 |
Nucleolus | 0.000128 | 0.000478 | 0.000039 | 0.000154 | 0.000078 | 0.000345 | 0.000041 | 0.000196 | 0.000086 | 0.000420 | 0.000041 | 0.000182 |
Nucleus | 0.005948 | 0.006252 | 0.002278 | 0.000798 | 0.002278 | 0.004728 | 0.000767 | 0.008306 | 0.002869 | 0.005586 | 0.000975 | 0.005902 |
Peroxisomes | 0.003558 | 0.004907 | 0.012162 | 0.002979 | 0.003140 | 0.005236 | 0.002964 | 0.004188 | 0.003207 | 0.005051 | 0.004229 | 0.003801 |
Vacuole | 0.027432 | 0.041804 | 0.042819 | 0.019521 | 0.022405 | 0.060280 | 0.018053 | 0.106874 | 0.023214 | 0.049883 | 0.021459 | 0.078906 |
Vacuole Periphery | 0.001451 | 0.003746 | 0.000144 | 0.001462 | 0.002972 | 0.003049 | 0.000811 | 0.000540 | 0.002728 | 0.003442 | 0.000719 | 0.000835 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.21 | -6.48 | -6.21 | -4.29 | 3.86 | -0.73 | -1.17 | 3.30 | 1.58 | 4.21 | -0.79 | -5.09 | -1.99 | -0.39 | 3.33 |
Bud Neck | 7.87 | 4.17 | 8.28 | 0.45 | 4.36 | 3.21 | 9.39 | 6.99 | 1.92 | -2.65 | 9.86 | 10.36 | 11.05 | -0.27 | 0.53 |
Bud Site | -0.85 | -10.01 | -12.33 | -14.44 | 0.69 | 2.68 | -19.25 | -0.70 | -3.10 | 17.53* | 3.18 | -21.71 | -7.64 | -9.86 | 14.40 |
Cell Periphery | 2.23 | -0.01 | -0.65 | -7.57 | -1.17 | 3.80 | 3.09 | 1.15 | -1.38 | -0.90 | 5.34 | 2.78 | 0.35 | -4.06 | -2.03 |
Cytoplasm | -3.72 | 9.33 | 0.06 | 4.72 | -11.27 | -0.26 | 4.91 | -1.37 | -0.72 | -6.08 | -3.62 | 7.37 | -0.91 | 2.90 | -8.43 |
Cytoplasmic Foci | 1.97 | 6.38 | 13.35 | 17.46 | 7.73 | -1.20 | 19.81 | 18.17 | 12.62 | -2.38 | -1.69 | 20.66 | 22.95 | 20.68 | 1.96 |
Eisosomes | 3.45 | 2.32 | 2.98 | -1.32 | 1.80 | 2.27 | 9.77 | 8.31 | 3.92 | -4.71 | 5.69 | 9.52 | 8.06 | 2.75 | -3.11 |
Endoplasmic Reticulum | -1.66 | 1.18 | 3.12 | 3.28 | 2.57 | 2.34 | 2.45 | 2.83 | 1.19 | 0.13 | 1.06 | 2.51 | 3.24 | 3.07 | 0.48 |
Endosome | -3.12 | 3.74 | 5.43 | 9.02 | 3.12 | -4.63 | 11.36 | 6.02 | 7.25 | -6.38 | -6.69 | 11.81 | 8.49 | 11.19 | -3.94 |
Golgi | -0.77 | 8.76 | 5.89 | 6.24 | -1.22 | 0.35 | 13.51 | 14.78 | 6.40 | 4.24 | 0.20 | 15.26 | 14.64 | 9.92 | 0.92 |
Lipid Particles | 0.14 | -2.09 | 1.00 | 0.67 | 2.58 | -1.07 | 4.63 | 2.88 | 1.44 | -3.32 | -1.36 | 2.55 | 2.06 | 1.91 | -0.84 |
Mitochondria | 0.02 | 1.82 | 0.42 | 0.51 | -1.23 | 0.71 | 3.16 | 5.65 | 3.58 | 0.70 | -0.19 | 3.58 | 2.30 | 2.24 | -0.56 |
Mitotic Spindle | -2.19 | -4.27 | -2.42 | -1.43 | -0.47 | -1.06 | 0.37 | -1.92 | -0.05 | -2.58 | -1.63 | -0.96 | -2.83 | -1.11 | -2.51 |
None | -1.54 | 3.82 | 2.84 | 1.70 | -0.66 | 0.85 | 2.64 | -2.78 | -2.86 | -3.01 | -1.55 | 3.40 | -2.61 | 0.30 | -3.00 |
Nuclear Periphery | -2.84 | -4.20 | -1.30 | 1.66 | 2.77 | -1.59 | 0.78 | -4.40 | -1.26 | -4.86 | -3.14 | -2.02 | -3.25 | 1.65 | -1.30 |
Nuclear Periphery Foci | 0.49 | -4.55 | 1.04 | 1.99 | 5.70 | -0.66 | 2.23 | -1.08 | 0.25 | -3.00 | -1.40 | -3.27 | -0.01 | 1.65 | 3.68 |
Nucleolus | -1.87 | 3.67 | -0.23 | 1.62 | -1.28 | -1.15 | 2.50 | -2.88 | 0.59 | -3.67 | -2.29 | 3.43 | -2.50 | 1.54 | -3.58 |
Nucleus | -0.18 | 1.77 | 6.05 | 3.92 | 4.89 | -1.85 | 9.25 | -7.77 | -2.61 | -9.53 | -2.83 | 7.54 | -5.78 | -0.65 | -8.60 |
Peroxisomes | -2.13 | -4.34 | 1.02 | 4.20 | 4.81 | -2.37 | 0.46 | -2.01 | 1.18 | -2.01 | -3.89 | -2.00 | -1.52 | 2.43 | 0.66 |
Vacuole | -3.50 | -4.84 | 0.87 | 4.58 | 5.21 | -7.33 | 1.78 | -19.30 | -8.15 | -19.47 | -8.86 | -1.57 | -17.98 | -8.40 | -15.43 |
Vacuole Periphery | -4.96 | 7.49 | 0.06 | 3.01 | -1.94 | -0.13 | 5.16 | 6.28 | 5.88 | 1.67 | -1.59 | 5.66 | 4.96 | 7.11 | -0.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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