Standard name | |
---|---|
Human Ortholog | |
Description | Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 2 | 3 |
Bud | 0 | 0 | 3 | 2 | 2 | 11 | 18 | 13 | 9 | 13 | 10 | 9 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | 6 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 11 | 11 | 6 | 8 | 10 | 17 | 9 | 0 | 2 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 14 | 17 | 12 | 21 | 30 | 29 | 15 | 17 | 10 | 7 | 42 | 108 | 130 | 6 | 21 | 15 | 0 | 0 | 0 | 6 | 6 | 1 |
Endoplasmic Reticulum | 3 | 3 | 5 | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 3 | 3 | 8 | 10 | 0 | 0 | 3 | 1 | 1 | 2 |
Endosome | 5 | 4 | 70 | 19 | 24 | 12 | 5 | 18 | 14 | 6 | 4 | 6 | 111 | 129 | 139 | 17 | 36 | 38 | 33 | 42 | 78 | 83 | 88 | 108 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 7 | 11 |
Mitochondria | 173 | 94 | 189 | 93 | 186 | 413 | 508 | 422 | 345 | 454 | 410 | 457 | 25 | 7 | 20 | 7 | 28 | 19 | 11 | 22 | 19 | 37 | 57 | 49 |
Nucleus | 4 | 6 | 10 | 14 | 28 | 32 | 28 | 37 | 26 | 19 | 23 | 42 | 19 | 23 | 32 | 0 | 6 | 6 | 2 | 5 | 2 | 13 | 14 | 6 |
Nuclear Periphery | 113 | 102 | 171 | 55 | 59 | 119 | 110 | 76 | 91 | 112 | 72 | 86 | 127 | 206 | 157 | 32 | 61 | 77 | 147 | 128 | 268 | 347 | 344 | 314 |
Nucleolus | 39 | 21 | 34 | 10 | 8 | 35 | 53 | 32 | 31 | 40 | 27 | 40 | 9 | 8 | 10 | 15 | 12 | 19 | 3 | 7 | 6 | 18 | 10 | 10 |
Peroxisomes | 0 | 0 | 5 | 4 | 1 | 8 | 2 | 6 | 4 | 3 | 0 | 2 | 16 | 7 | 22 | 0 | 0 | 3 | 2 | 2 | 9 | 6 | 10 | 18 |
SpindlePole | 2 | 2 | 15 | 7 | 8 | 13 | 14 | 16 | 17 | 6 | 10 | 12 | 20 | 28 | 35 | 3 | 3 | 3 | 8 | 7 | 23 | 30 | 26 | 23 |
Vac/Vac Membrane | 24 | 61 | 41 | 10 | 35 | 19 | 35 | 24 | 16 | 31 | 20 | 35 | 90 | 168 | 177 | 18 | 58 | 50 | 6 | 13 | 4 | 21 | 17 | 19 |
Unique Cell Count | 210 | 200 | 372 | 164 | 265 | 496 | 600 | 514 | 413 | 516 | 448 | 516 | 369 | 543 | 579 | 58 | 157 | 155 | 221 | 240 | 443 | 589 | 606 | 578 |
Labelled Cell Count | 364 | 295 | 560 | 232 | 368 | 695 | 815 | 679 | 576 | 711 | 604 | 705 | 463 | 687 | 727 | 101 | 233 | 240 | 221 | 240 | 443 | 589 | 606 | 578 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.6 | 5.1 | 5.0 | 4.8 | 4.1 | 4.0 | 4.1 | 3.9 | 3.7 | 3.5 | 3.6 | 6.7 | 6.6 | 6.3 | 6.5 | 6.7 | 6.9 | 6.8 | 6.9 | 7.2 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.1 | 2.0 | 1.8 | 1.2 | 1.4 | 1.4 | 1.3 | 0.9 | 1.2 | 0.9 | 1.1 | 1.2 | 1.2 | 1.0 | 1.4 | 1.3 | 1.1 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.09 | -0.32 | -0.35 | -0.32 | -0.39 | -0.48 | -0.55 | -0.52 | 0.37 | 0.36 | 0.29 | 0.35 | 0.38 | 0.43 | 0.41 | 0.41 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.0 | 0.5 | 0.3 | 0.9 | 1.3 | -0.1 | -0.4 | -1.3 | -2.3 | 3.9 | 7.0 | 7.8 | 0 | 4.1 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 1.8 | 1.9 | 1.9 | 1.1 | 1.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -13.2 | -17.9 | -17.2 | -5.5 | -7.0 | -8.2 |
Nucleus | 3.0 | 4.1 | 2.6 | 1.5 | 3.0 | 2.4 | 0.8 | 1.8 | 3.4 | 1.7 | 1.2 | 2.1 | 0 | 0 | 0 |
Nuclear Periphery | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -2.4 | -6.0 | 1.3 | -1.5 | 0.8 |
Nucleolus | -1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.9 | -5.4 | -5.3 | 3.7 | -0.6 | 1.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 2.2 | 0 | 0 | 0 |
SpindlePole | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0.8 | 1.4 | 0 | -1.2 | -1.2 |
Vacuole | -1.8 | 0.8 | -4.1 | -2.9 | -3.6 | -3.9 | -2.7 | -3.6 | -2.2 | 4.8 | 7.0 | 7.0 | 4.1 | 7.0 | 5.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1791 | 3.0889 | 2.9053 | 2.5575 | 3.0297 | 2.5496 | 2.2441 | 1.891 | 1.8784 | 1.5414 | 1.7881 | 1.7851 | 1.0812 | 1.6779 | 1.09 | 1.5295 | 1.5572 | 1.4556 |
Actin | 0.093 | 0.0013 | 0.0072 | 0.0183 | 0.0267 | 0.0023 | 0.0127 | 0.0008 | 0.0082 | 0.0117 | 0.0025 | 0.0016 | 0.0278 | 0.0074 | 0.0091 | 0.0081 | 0.0008 | 0.0067 |
Bud | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 |
Bud Neck | 0.0021 | 0.0027 | 0.0008 | 0.0012 | 0.0005 | 0.0008 | 0.0021 | 0.0003 | 0.0008 | 0.0015 | 0.0009 | 0.0008 | 0.0005 | 0.0011 | 0.0006 | 0.0003 | 0.0002 | 0.0004 |
Bud Periphery | 0.0011 | 0.0003 | 0.0004 | 0.0008 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0006 | 0.0013 | 0.0007 | 0.0001 | 0.0004 | 0.001 | 0.0006 | 0.001 | 0.0003 | 0.0004 |
Bud Site | 0.003 | 0.0191 | 0.0022 | 0.0007 | 0.0001 | 0.0002 | 0.0033 | 0.0003 | 0.0007 | 0.0037 | 0.0018 | 0.0001 | 0.0025 | 0.001 | 0.0087 | 0.0013 | 0.0002 | 0.0002 |
Cell Periphery | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0254 | 0.0212 | 0.0066 | 0.0096 | 0.0068 | 0.0021 | 0.003 | 0.0028 | 0.003 | 0.0009 | 0.0007 | 0.0008 | 0.0042 | 0.0014 | 0.003 | 0.0062 | 0.0005 | 0.003 |
Cytoplasmic Foci | 0.0214 | 0.006 | 0.0054 | 0.0056 | 0.0064 | 0.0033 | 0.0058 | 0.0008 | 0.0048 | 0.0076 | 0.0039 | 0.0007 | 0.0346 | 0.0031 | 0.016 | 0.0079 | 0.0032 | 0.0064 |
Eisosomes | 0.0009 | 0.0001 | 0.0008 | 0.0003 | 0.0019 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0121 | 0.017 | 0.0058 | 0.0187 | 0.0028 | 0.0063 | 0.005 | 0.0024 | 0.0048 | 0.0038 | 0.0017 | 0.0014 | 0.0058 | 0.0072 | 0.0037 | 0.007 | 0.0035 | 0.0029 |
Endosome | 0.0392 | 0.0152 | 0.0097 | 0.0117 | 0.0078 | 0.0114 | 0.0172 | 0.0033 | 0.0063 | 0.0197 | 0.0117 | 0.0052 | 0.0524 | 0.0103 | 0.0133 | 0.0218 | 0.0571 | 0.017 |
Golgi | 0.0135 | 0.0024 | 0.0028 | 0.0043 | 0.0047 | 0.0021 | 0.0055 | 0.0009 | 0.0021 | 0.009 | 0.004 | 0.0011 | 0.0194 | 0.0059 | 0.0097 | 0.0043 | 0.0186 | 0.0079 |
Lipid Particles | 0.0319 | 0.051 | 0.0466 | 0.0437 | 0.0625 | 0.0473 | 0.0467 | 0.0145 | 0.0137 | 0.0238 | 0.0415 | 0.0085 | 0.1239 | 0.0497 | 0.0427 | 0.0365 | 0.1625 | 0.0276 |
Mitochondria | 0.0072 | 0.0016 | 0.0068 | 0.0058 | 0.0024 | 0.0046 | 0.0071 | 0.0012 | 0.0072 | 0.0228 | 0.0094 | 0.0014 | 0.0034 | 0.0102 | 0.0083 | 0.0104 | 0.0065 | 0.0019 |
None | 0.0502 | 0.0292 | 0.0625 | 0.0093 | 0.2028 | 0.0042 | 0.0019 | 0.001 | 0.0019 | 0.0017 | 0.0014 | 0.0046 | 0.0054 | 0.0043 | 0.0034 | 0.0038 | 0.0007 | 0.0007 |
Nuclear Periphery | 0.5409 | 0.6702 | 0.6789 | 0.7501 | 0.5914 | 0.7857 | 0.7895 | 0.7823 | 0.8042 | 0.7156 | 0.7643 | 0.8467 | 0.6573 | 0.8135 | 0.7707 | 0.8127 | 0.6752 | 0.8553 |
Nucleolus | 0.0056 | 0.0229 | 0.011 | 0.0039 | 0.0043 | 0.0208 | 0.0129 | 0.0262 | 0.0141 | 0.0287 | 0.0274 | 0.021 | 0.0039 | 0.012 | 0.0093 | 0.0042 | 0.0141 | 0.0092 |
Nucleus | 0.0576 | 0.0747 | 0.0478 | 0.0451 | 0.0064 | 0.0511 | 0.0375 | 0.1007 | 0.0722 | 0.0515 | 0.0596 | 0.058 | 0.0034 | 0.0283 | 0.0237 | 0.011 | 0.0132 | 0.0144 |
Peroxisomes | 0.006 | 0.0019 | 0.0028 | 0.0063 | 0.0069 | 0.0032 | 0.007 | 0.0004 | 0.0045 | 0.0095 | 0.004 | 0.0003 | 0.0171 | 0.0013 | 0.0059 | 0.0021 | 0.0019 | 0.004 |
Punctate Nuclear | 0.0681 | 0.0396 | 0.082 | 0.0365 | 0.0505 | 0.0362 | 0.0259 | 0.0493 | 0.0352 | 0.0557 | 0.0475 | 0.0399 | 0.0237 | 0.0148 | 0.0492 | 0.0271 | 0.0173 | 0.0308 |
Vacuole | 0.0037 | 0.0055 | 0.0022 | 0.0026 | 0.0013 | 0.0056 | 0.0029 | 0.001 | 0.0021 | 0.01 | 0.0034 | 0.0011 | 0.0023 | 0.0024 | 0.0018 | 0.0022 | 0.005 | 0.0014 |
Vacuole Periphery | 0.0161 | 0.0173 | 0.0174 | 0.0253 | 0.0133 | 0.0117 | 0.0127 | 0.0113 | 0.0134 | 0.0201 | 0.0127 | 0.0066 | 0.0114 | 0.0244 | 0.0195 | 0.0314 | 0.0188 | 0.0093 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.6625 | 4.3262 | 5.8854 | 9.6015 | 7.3204 | 8.0614 | 12.9404 | 10.8251 | 9.7602 | 10.549 |
Translational Efficiency | 0.7232 | 1.3244 | 0.9356 | 0.5987 | 0.8002 | 0.5581 | 0.4166 | 0.569 | 0.703 | 0.7255 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1679 | 251 | 2184 | 1290 | 1725 | 1898 | 1231 | 99 | 3404 | 2149 | 3415 | 1389 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 720.13 | 891.16 | 860.42 | 884.08 | 745.10 | 793.02 | 941.54 | 1029.94 | 732.78 | 804.48 | 889.66 | 894.48 |
Standard Deviation | 93.23 | 109.15 | 111.96 | 124.15 | 90.45 | 97.21 | 123.54 | 131.53 | 92.68 | 103.59 | 122.62 | 130.21 |
Intensity Change Log 2 | — | 0.307427 | 0.256784 | 0.295920 | — | 0.089923 | 0.337588 | 0.467054 | — | 0.200919 | 0.298440 | 0.385479 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000186 | 0.000125 | 0.000350 | 0.000753 | 0.000106 | 0.000161 | 0.000281 | 0.000078 | 0.000146 | 0.000157 | 0.000325 | 0.000705 |
Bud Neck | 0.000325 | 0.000467 | 0.002284 | 0.002774 | 0.000114 | 0.000359 | 0.000930 | 0.001327 | 0.000218 | 0.000371 | 0.001796 | 0.002671 |
Bud Site | 0.000459 | 0.000201 | 0.001328 | 0.002572 | 0.000048 | 0.001009 | 0.000195 | 0.004917 | 0.000251 | 0.000915 | 0.000920 | 0.002739 |
Cell Periphery | 0.000057 | 0.000017 | 0.000132 | 0.000049 | 0.000033 | 0.000039 | 0.000016 | 0.000012 | 0.000045 | 0.000037 | 0.000090 | 0.000046 |
Cytoplasm | 0.007881 | 0.000793 | 0.021729 | 0.004496 | 0.002782 | 0.005355 | 0.001464 | 0.002015 | 0.005297 | 0.004822 | 0.014424 | 0.004320 |
Cytoplasmic Foci | 0.008469 | 0.001096 | 0.003190 | 0.002829 | 0.002202 | 0.005451 | 0.000143 | 0.003353 | 0.005293 | 0.004943 | 0.002092 | 0.002866 |
Eisosomes | 0.000015 | 0.000004 | 0.000029 | 0.000011 | 0.000010 | 0.000015 | 0.000008 | 0.000006 | 0.000012 | 0.000013 | 0.000021 | 0.000010 |
Endoplasmic Reticulum | 0.005483 | 0.003899 | 0.004288 | 0.008409 | 0.003670 | 0.003822 | 0.003638 | 0.003963 | 0.004564 | 0.003831 | 0.004054 | 0.008092 |
Endosome | 0.008240 | 0.003671 | 0.002227 | 0.006397 | 0.002614 | 0.006789 | 0.000682 | 0.008087 | 0.005389 | 0.006425 | 0.001670 | 0.006517 |
Golgi | 0.004594 | 0.000960 | 0.000346 | 0.002497 | 0.001006 | 0.003227 | 0.000134 | 0.000889 | 0.002776 | 0.002963 | 0.000270 | 0.002382 |
Lipid Particles | 0.132019* | 0.027493 | 0.004060 | 0.003976 | 0.070763 | 0.065232 | 0.001114 | 0.002255 | 0.100977* | 0.060824 | 0.002998 | 0.003853 |
Mitochondria | 0.008649 | 0.001007 | 0.004078 | 0.004389 | 0.003658 | 0.007924 | 0.001191 | 0.000838 | 0.006120 | 0.007116 | 0.003037 | 0.004136 |
Mitotic Spindle | 0.020984 | 0.047017 | 0.065195 | 0.161311* | 0.011872 | 0.030811 | 0.086164 | 0.305580* | 0.016367 | 0.032704 | 0.072754 | 0.171594* |
None | 0.005570 | 0.001037 | 0.011325 | 0.000444 | 0.002895 | 0.002783 | 0.000474 | 0.000056 | 0.004214 | 0.002579 | 0.007413 | 0.000416 |
Nuclear Periphery | 0.054169 | 0.069309 | 0.145720* | 0.176814* | 0.069572 | 0.071444 | 0.181945* | 0.176662* | 0.061975 | 0.071195 | 0.158778* | 0.176803* |
Nuclear Periphery Foci | 0.715334* | 0.827031* | 0.393076* | 0.575047* | 0.806153* | 0.746728* | 0.637129* | 0.459134* | 0.761357* | 0.756107* | 0.481049* | 0.566786* |
Nucleolus | 0.013797 | 0.005681 | 0.091161 | 0.007837 | 0.011246 | 0.021500 | 0.037970 | 0.001352 | 0.012504 | 0.019653 | 0.071987 | 0.007374 |
Nucleus | 0.009873 | 0.008331 | 0.245804* | 0.020674 | 0.009221 | 0.023237 | 0.044312 | 0.004242 | 0.009542 | 0.021496 | 0.173173* | 0.019502 |
Peroxisomes | 0.001275 | 0.000158 | 0.000641 | 0.000489 | 0.000657 | 0.001051 | 0.000198 | 0.000574 | 0.000962 | 0.000947 | 0.000481 | 0.000495 |
Vacuole | 0.001583 | 0.000994 | 0.002386 | 0.014133 | 0.000858 | 0.001697 | 0.001358 | 0.019684 | 0.001216 | 0.001615 | 0.002015 | 0.014528 |
Vacuole Periphery | 0.001039 | 0.000707 | 0.000650 | 0.004101 | 0.000519 | 0.001365 | 0.000654 | 0.004977 | 0.000775 | 0.001288 | 0.000651 | 0.004163 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.42 | -3.82 | -1.93 | -2.17 | -1.34 | -2.37 | -6.53 | 0.11 | 1.71 | 5.17 | -0.23 | -7.31 | -2.07 | -2.05 | -1.37 |
Bud Neck | -0.68 | -9.46 | -7.19 | -6.32 | -2.09 | -5.03 | -4.16 | -3.00 | -2.47 | -0.85 | -3.19 | -10.44 | -7.65 | -7.21 | -3.10 |
Bud Site | 1.11 | -2.05 | -3.75 | -4.19 | -2.01 | -1.72 | -1.93 | -1.63 | -1.36 | -1.56 | -1.30 | -2.56 | -4.44 | -2.96 | -3.05 |
Cell Periphery | 8.92 | -2.61 | 0.75 | -5.06 | 2.77 | -1.33 | 3.35 | 3.30 | 5.77 | 0.73 | 1.84 | -2.47 | -0.58 | -1.72 | 2.16 |
Cytoplasm | 5.95 | -7.12 | 1.51 | -5.07 | 8.90 | -2.47 | 1.42 | -0.24 | 1.17 | -0.90 | 0.46 | -7.56 | -0.28 | -0.66 | 6.63 |
Cytoplasmic Foci | 6.87 | 4.02 | 5.17 | -2.99 | 1.54 | -4.56 | 5.25 | -0.49 | 1.24 | -1.67 | 0.65 | 4.30 | 3.69 | 3.12 | -0.44 |
Eisosomes | 7.58 | -8.23 | 1.83 | -4.87 | 9.29 | -4.36 | 0.96 | 1.05 | 2.88 | 0.69 | -1.45 | -8.37 | 0.56 | 1.60 | 7.12 |
Endoplasmic Reticulum | 2.26 | 1.97 | -3.30 | -4.69 | -4.60 | -0.42 | -0.74 | -0.47 | -0.33 | -0.20 | 2.06 | 0.93 | -4.34 | -5.14 | -4.76 |
Endosome | 3.68 | 6.65 | 1.59 | -2.65 | -6.28 | -4.70 | 5.89 | -1.43 | -0.30 | -1.94 | -1.13 | 7.52 | -1.69 | -0.34 | -7.53 |
Golgi | 3.83 | 6.80 | 2.41 | -1.38 | -2.76 | -3.63 | 2.97 | 0.26 | 3.05 | -1.23 | -0.23 | 7.24 | 0.73 | 0.83 | -2.94 |
Lipid Particles | 14.16 | 24.03 | 24.13 | 4.47 | 0.84 | 1.12 | 18.79 | 18.28 | 18.55 | -1.71 | 8.98 | 29.88 | 29.48 | 18.50 | -0.82 |
Mitochondria | 7.74 | 4.45 | 3.10 | -3.40 | -0.18 | -3.94 | 6.14 | 5.28 | 6.52 | 1.11 | -0.96 | 5.49 | 1.89 | 2.32 | -1.07 |
Mitotic Spindle | -3.05 | -11.48 | -18.41 | -10.51 | -11.24 | -6.32 | -12.75 | -8.19 | -7.68 | -6.18 | -6.02 | -17.44 | -20.68 | -17.88 | -12.19 |
None | 4.12 | -4.29 | 6.13 | 0.61 | 9.91 | -0.14 | 3.25 | 3.59 | 5.47 | 1.63 | 2.18 | -3.77 | 6.73 | 5.12 | 9.81 |
Nuclear Periphery | -2.47 | -19.01 | -21.02 | -13.08 | -5.65 | -0.37 | -19.27 | -4.82 | -4.76 | 0.39 | -3.07 | -25.86 | -20.73 | -18.24 | -3.50 |
Nuclear Periphery Foci | -7.56 | 32.61* | 13.45 | 16.99* | -16.08 | 7.11 | 17.60 | 10.69 | 8.80 | 5.54 | 1.08 | 38.70 | 21.37 | 18.96 | -8.05 |
Nucleolus | 3.16 | -22.78* | 2.87 | -1.34 | 25.24* | -5.34 | -9.37 | 7.03 | 10.46 | 13.30 | -4.35 | -24.71* | 3.13 | 6.42 | 26.20* |
Nucleus | 0.46 | -35.15 | -5.42 | -4.28 | 30.79 | -6.12 | -11.17 | 1.08 | 5.41 | 10.15 | -6.02 | -34.71 | -5.63 | -0.72 | 28.22 |
Peroxisomes | 4.81 | 2.39 | 2.80 | -2.24 | 1.02 | -2.38 | 5.77 | 0.39 | 1.68 | -1.18 | 0.22 | 3.19 | 2.33 | 2.01 | 0.05 |
Vacuole | 0.86 | -2.04 | -8.09 | -8.12 | -7.53 | -1.71 | -1.77 | -2.06 | -1.97 | -1.90 | -0.97 | -2.97 | -8.51 | -8.27 | -7.76 |
Vacuole Periphery | 0.64 | 2.35 | -4.27 | -4.00 | -4.93 | -2.89 | -0.34 | -1.47 | -1.17 | -1.40 | -2.05 | 0.72 | -4.89 | -4.01 | -5.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|