Standard name
Human Ortholog
Description Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles; interaction with Atg19p during the Cvt pathway requires phosphorylation by Hrr25p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0.09 0.05 0.07 0.11 0.14 0.12 0.12 0.19 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.08 0.06 0.07 0.05 0.09 0.06 0 0.06 0.06 0.07 0 0.06 0.11 0.05 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.06 0.06 0.06 0.07 0 0.05 0 0 0 0 0.06 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.75 0.8 0.88 0.57 0.48 0.4 0.35 0.29 0.34 0.26 0.39 0.75 0.73 0.77 0.35 0.37 0.36 0.49 0.57 0.5 0.61 0.58 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0.05 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0.11 0.06 0.12 0.24 0.23 0.27 0.3 0.36 0.36 0 0 0 0.06 0 0 0.11 0.07 0.2 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.07 0.07 0 0 0 0.06 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.17 0.16 0.11 0.08 0.36 0.39 0.42 0.48 0.45 0.38 0.38 0.32 0.23 0.24 0.2 0.58 0.56 0.54 0.2 0.25 0.13 0.22 0.24 0.23
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 1 0 2 0 0 0 0 1 0 0 0 0 0 2 1 5 1 0 2
Bud 6 3 6 1 13 7 16 21 21 22 15 25 4 3 3 15 11 8 4 5 2 3 7 2
Bud Neck 7 8 5 1 12 8 15 9 13 11 4 8 20 19 8 17 26 11 1 4 0 1 1 5
Bud Site 7 4 1 0 3 7 13 11 9 13 3 7 2 0 0 4 15 8 – – – – – –
Cell Periphery 1 4 0 1 0 0 0 0 0 0 0 1 0 0 0 0 2 2 0 0 0 0 1 0
Cytoplasm 153 165 105 121 82 70 86 69 44 65 32 53 233 191 222 104 92 77 111 136 66 90 119 153
Endoplasmic Reticulum 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 9 6 10 4 1 0 0 0 1
Endosome 0 12 3 3 6 6 2 8 3 2 5 1 1 4 4 19 8 17 11 7 6 5 7 7
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1 1 1 1 0 0 0 1
Mitochondria 32 4 5 15 9 18 52 45 41 57 45 49 1 0 0 17 11 6 25 15 26 2 7 7
Nucleus 2 0 0 0 0 0 6 0 0 2 1 1 0 0 0 4 0 0 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 3 0 2 0 2 8 5 6 3 12 2 6 1 3 0 14 7 9 1 0 1 0 0 0
Peroxisomes 0 4 3 5 4 10 15 7 4 8 8 6 0 0 0 7 4 10 1 0 0 0 0 0
SpindlePole 35 35 15 11 52 57 91 95 68 72 47 43 71 64 58 169 138 115 45 59 17 31 48 58
Vac/Vac Membrane 0 1 4 0 2 0 2 2 3 1 2 1 0 0 0 4 4 1 6 2 0 1 4 8
Unique Cell Count 209 219 131 138 144 145 215 197 152 190 125 135 310 262 287 293 246 213 227 238 133 147 206 253
Labelled Cell Count 249 243 149 159 185 193 304 275 209 265 164 201 336 284 296 384 325 275 227 238 133 147 206 253


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.9 5.3 3.7 3.9 4.2 3.7 3.6 3.8 3.4 3.2 3.0 5.0 5.1 5.0 6.0 5.4 5.7 5.1 5.5 5.6
Std Deviation (1e-4) 0.7 0.8 3.9 1.9 1.6 3.3 2.4 1.8 2.2 1.8 1.5 0.8 0.9 1.0 1.1 2.3 1.5 2.0 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.52 -0.43 -0.34 -0.49 -0.55 -0.47 -0.65 -0.7 -0.8 -0.07 -0.04 -0.08 0.19 0.03 0.12 -0.04 0.06 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.647 -0.3593 -0.5925 -0.9749 -0.2091 -0.531 -0.3507 -0.2888 -0.4743 -0.5676 -0.3423 -0.2344 -0.9386 -1.0375 -1.3248 -1.346 -0.9356 -1.0816
Actin 0.0258 0.0033 0.0029 0.0001 0.0312 0.0011 0.0213 0.0017 0.014 0.0333 0.0012 0.0218 0.0184 0.0034 0.0006 0.0001 0.0371 0.0216
Bud 0.0002 0.0037 0.0026 0.0054 0.0019 0.0003 0.0004 0.0027 0.001 0.0008 0.0004 0.0005 0.0122 0.0121 0.0037 0 0.0004 0.0001
Bud Neck 0.0008 0.001 0.001 0.0009 0.0005 0.0026 0.0007 0.0009 0.0007 0.0006 0.0023 0.0011 0.0073 0.0014 0.0003 0.0041 0.0189 0.0003
Bud Periphery 0.0006 0.0044 0.0048 0.0102 0.007 0.0005 0.0006 0.0021 0.001 0.0014 0.0004 0.0006 0.0081 0.0059 0.0024 0 0.0006 0.0002
Bud Site 0.0007 0.0062 0.0046 0.0011 0.0032 0.001 0.0018 0.0073 0.0088 0.0038 0.0047 0.0005 0.0035 0.0259 0.0032 0.0001 0.0009 0.0002
Cell Periphery 0.0001 0.0002 0.0005 0.0002 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0 0.0007 0.0014 0.0002 0.0001 0.0002 0
Cytoplasm 0.0143 0.0335 0.0208 0.0407 0.0194 0.0263 0.0301 0.0401 0.0287 0.0093 0.0559 0.062 0.0374 0.0568 0.0523 0.0106 0.0535 0.0946
Cytoplasmic Foci 0.3001 0.2906 0.2925 0.2263 0.2836 0.3297 0.2287 0.1465 0.1493 0.4575 0.2089 0.2379 0.2084 0.1977 0.1484 0.2814 0.3234 0.2561
Eisosomes 0.0004 0.0004 0.0002 0 0.0004 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0001 0.0002 0.0003
Endoplasmic Reticulum 0.0007 0.0003 0.0007 0.0006 0.0016 0.0004 0.002 0.0009 0.0008 0.0005 0.001 0.0015 0.0011 0.0008 0.0014 0.0001 0.0032 0.0006
Endosome 0.0139 0.023 0.012 0.0049 0.013 0.0155 0.0273 0.0194 0.0157 0.0477 0.036 0.0092 0.0378 0.0129 0.0285 0.0057 0.0376 0.0308
Golgi 0.0034 0.0022 0.0024 0.001 0.0048 0.0014 0.0049 0.0023 0.0025 0.0117 0.0023 0.0016 0.006 0.0013 0.0012 0.0009 0.0059 0.0067
Lipid Particles 0.0327 0.0354 0.0335 0.0096 0.0188 0.025 0.0231 0.0268 0.0195 0.0514 0.0088 0.0164 0.0576 0.0172 0.0224 0.0165 0.0368 0.0154
Mitochondria 0.001 0.0011 0.0023 0.0013 0.0074 0.0009 0.0018 0.0011 0.0016 0.0045 0.0005 0.0007 0.0116 0.0039 0.0012 0.0001 0.001 0.0087
None 0.5421 0.4948 0.5457 0.6097 0.5392 0.5363 0.5778 0.6982 0.673 0.255 0.5667 0.5662 0.515 0.6123 0.6821 0.6487 0.449 0.5069
Nuclear Periphery 0.001 0.0003 0.0008 0.0002 0.0069 0.0006 0.0127 0.0009 0.0042 0.0003 0.0008 0.0024 0.0061 0.0011 0.0045 0 0.0036 0.0003
Nucleolus 0.0053 0.0079 0.0014 0.0004 0.0005 0.0015 0.0017 0.0043 0.0061 0.0015 0.0019 0.0006 0.0034 0.0071 0.0015 0.0002 0.0003 0.0002
Nucleus 0.0014 0.0015 0.001 0.0006 0.0021 0.0011 0.0085 0.0024 0.01 0.0004 0.0034 0.0043 0.0115 0.002 0.0057 0.0002 0.0015 0.0003
Peroxisomes 0.0354 0.0249 0.0419 0.0693 0.04 0.0268 0.025 0.0139 0.0192 0.1096 0.065 0.0212 0.0227 0.0067 0.0088 0.0278 0.0173 0.0238
Punctate Nuclear 0.0196 0.0632 0.026 0.0164 0.0164 0.0257 0.0203 0.0231 0.0404 0.0081 0.0374 0.0501 0.0251 0.0226 0.029 0.0031 0.0069 0.0321
Vacuole 0.0005 0.0019 0.0019 0.001 0.001 0.0025 0.0091 0.0044 0.0026 0.002 0.0019 0.0009 0.0044 0.0062 0.002 0.0001 0.001 0.0008
Vacuole Periphery 0.0001 0.0002 0.0003 0.0001 0.001 0.0005 0.0017 0.0005 0.0003 0.0004 0.0002 0.0002 0.0013 0.0012 0.0004 0 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.4083 22.9206 22.2864 32.4877 32.0971 28.2768 31.5659 33.7103 43.0762 34.5018
Translational Efficiency 0.6259 0.7683 0.7398 0.7324 0.5686 1.1852 0.8443 0.7704 0.7967 0.7146

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
924 530 1491 1511 193 1891 684 599 1117 2421 2175 2110

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 621.46 705.31 730.93 759.10 708.72 663.49 837.00 802.46 636.54 672.65 764.29 771.41
Standard Deviation 67.22 74.94 90.07 115.67 80.87 74.43 93.74 104.36 77.18 76.53 103.68 114.26
Intensity Change Log 2 — 0.182596 0.234072 0.288628 — -0.095141 0.240012 0.179214 — 0.041290 0.237240 0.231367

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000227 0.000383 0.000195 0.000640 0.000209 0.000421 0.000841 0.001808 0.000224 0.000412 0.000399 0.000971
Bud Neck 0.055723 0.055677 0.025852 0.033743 0.057205 0.050274 0.018493 0.023771 0.055979 0.051457 0.023538 0.030912
Bud Site 0.037881 0.035264 0.018125 0.058490 0.036414 0.023400 0.050279 0.097887 0.037627 0.025997 0.028237 0.069675
Cell Periphery 0.000299 0.000251 0.000378 0.000500 0.000315 0.000168 0.000237 0.000206 0.000302 0.000186 0.000334 0.000416
Cytoplasm 0.191287* 0.185929* 0.287085* 0.324343* 0.176373* 0.317975* 0.232300* 0.263034* 0.188710* 0.289068* 0.269856* 0.306938*
Cytoplasmic Foci 0.432536* 0.399013* 0.044519 0.081874 0.452738* 0.332894* 0.078079 0.124855 0.436027* 0.347369* 0.055073 0.094075
Eisosomes 0.000187 0.000164 0.000076 0.000121 0.000181 0.000158 0.000093 0.000105 0.000186 0.000159 0.000082 0.000116
Endoplasmic Reticulum 0.000324 0.000504 0.000806 0.001680 0.000288 0.000354 0.002330 0.001954 0.000318 0.000387 0.001285 0.001758
Endosome 0.066498 0.065433 0.008521 0.024426 0.054841 0.038748 0.016187 0.022682 0.064483 0.044590 0.010931 0.023931
Golgi 0.013801 0.016226 0.000578 0.005646 0.013731 0.008157 0.000956 0.004493 0.013789 0.009923 0.000697 0.005319
Lipid Particles 0.016661 0.008786 0.001928 0.005247 0.007737 0.008080 0.003899 0.002659 0.015119 0.008235 0.002548 0.004512
Mitochondria 0.009544 0.018543 0.001374 0.006726 0.001687 0.014286 0.001074 0.010863 0.008187 0.015218 0.001279 0.007900
Mitotic Spindle 0.004200 0.014232 0.010034 0.039610 0.010905 0.008653 0.022031 0.052578 0.005359 0.009874 0.013807 0.043292
None 0.004858 0.002532 0.016164 0.005970 0.004626 0.003038 0.004932 0.007706 0.004818 0.002927 0.012632 0.006463
Nuclear Periphery 0.000257 0.000236 0.001212 0.001279 0.000200 0.000284 0.001466 0.000928 0.000247 0.000273 0.001292 0.001179
Nuclear Periphery Foci 0.007178 0.005521 0.001758 0.002207 0.025175 0.000844 0.003511 0.004124 0.010287 0.001868 0.002309 0.002751
Nucleolus 0.008809 0.003237 0.008708 0.006086 0.008875 0.004194 0.006084 0.002562 0.008820 0.003985 0.007883 0.005086
Nucleus 0.041682 0.055301 0.541703* 0.287389* 0.040162 0.088086 0.486609* 0.166094* 0.041420 0.080909 0.524377* 0.252955*
Peroxisomes 0.080629 0.091732 0.009987 0.018625 0.090891 0.054426 0.023071 0.095093 0.082402 0.062593 0.014102 0.040333
Vacuole 0.026278 0.038910 0.020593 0.092202 0.016653 0.043983 0.047271 0.113537 0.024615 0.042872 0.028983 0.098259
Vacuole Periphery 0.001142 0.002125 0.000403 0.003197 0.000794 0.001576 0.000257 0.003061 0.001082 0.001696 0.000357 0.003159

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -1.14 -5.62 -2.95 -3.10 -2.58 -4.48 -7.00 -5.58 -2.15 -2.90 -3.69 -8.70 -5.38 -3.53
Bud Neck 0.01 4.31 4.92 3.93 0.78 0.18 2.27 2.56 6.97 0.84 0.59 5.14 5.77 7.45 1.00
Bud Site 0.48 3.78 -3.16 -3.30 -6.98 1.89 -2.39 -4.91 -8.01 -2.95 3.50 1.29 -5.36 -9.55 -6.99
Cell Periphery 0.92 -0.51 -0.64 -1.36 -0.13 2.45 1.41 2.18 -0.90 1.94 3.99 0.24 0.34 -3.43 0.11
Cytoplasm 0.28 -6.84 -9.01 -8.00 -2.37 -7.79 -1.46 -3.12 7.17 -2.74 -11.18 -6.11 -9.06 2.42 -3.62
Cytoplasmic Foci 2.37 37.73 34.88 26.87 -4.54 5.73 17.09 16.87 28.64 -0.55 9.20 41.02 38.80 42.68 -3.92
Eisosomes 1.14 6.60 4.53 3.38 -5.35 0.79 1.58 1.56 2.53 -0.04 1.84 6.20 4.66 5.30 -4.06
Endoplasmic Reticulum -1.63 -10.42 -14.01 -10.90 -5.88 -0.35 -12.63 -12.13 -12.25 0.44 -1.11 -15.92 -18.22 -17.89 -3.86
Endosome 0.20 12.36 9.59 7.85 -5.13 1.84 4.07 4.35 6.65 0.64 4.55 12.64 10.70 9.24 -3.92
Golgi -0.87 10.93 7.18 5.58 -3.19 2.87 6.93 6.17 7.38 -1.96 3.17 12.43 8.94 7.46 -3.54
Lipid Particles 4.14 8.59 6.90 3.46 -4.16 -0.23 2.29 3.55 7.64 2.74 4.72 8.55 7.50 6.24 -2.75
Mitochondria -2.30 5.53 3.09 3.89 -2.72 -10.00 1.53 -1.32 4.34 -1.62 -4.13 5.63 2.65 7.30 -3.15
Mitotic Spindle -3.21 -5.89 -11.75 -8.20 -7.29 0.67 -3.98 -4.69 -6.55 -1.07 -2.35 -7.90 -13.00 -12.00 -6.46
None 2.37 -4.80 0.25 -2.59 5.29 1.20 -0.11 0.20 -1.26 0.43 2.23 -4.49 0.30 -2.55 5.17
Nuclear Periphery 0.44 -20.34 -16.49 -16.53 -5.66 -2.60 -16.79 -13.79 -13.17 1.16 -1.15 -25.88 -20.16 -19.93 -4.52
Nuclear Periphery Foci 0.55 3.14 3.12 1.49 -0.32 3.75 2.66 2.98 -4.24 1.27 5.24 3.76 4.16 -3.00 0.75
Nucleolus 3.25 -3.15 0.28 -3.60 3.85 2.36 0.39 2.12 -0.72 3.95 3.65 -2.53 1.60 -3.03 5.32
Nucleus -2.57 -49.29 -27.79 -23.06 21.70 -6.45 -25.54 -13.35 -10.80 12.99 -10.29 -55.08 -30.79 -24.76 24.91
Peroxisomes -1.32 13.74 12.44 10.71 -2.72 3.62 6.45 3.30 0.20 -4.12 4.03 14.76 11.37 10.16 -4.69
Vacuole -3.61 -1.59 -22.02 -18.71 -21.64 -7.19 -10.03 -17.88 -15.85 -11.63 -7.79 -7.47 -27.88 -24.69 -23.61
Vacuole Periphery -2.78 5.73 -1.72 0.52 -3.66 -3.52 2.91 -0.83 0.47 -1.63 -3.46 6.57 -1.77 0.05 -3.98
Standard name
Human Ortholog
Description Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles; interaction with Atg19p during the Cvt pathway requires phosphorylation by Hrr25p
Localization
Cell Percentages spindle pole (17%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg19

Atg19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available