Standard name
Human Ortholog
Description Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 – – 0.05 0 0.05 0
Bud – – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.08 0.07 0.08 0.08
Bud Site – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0.27 0.32 0.26 0.19 0.19 0.11 0.15 – 0.15 0.18 0.55 0.6 0.53 0.59 0.47 0.52 – – 0.34 0.15 0.34 0.3
Cytoplasm – – 0.92 0.97 0.91 0.9 0.87 0.9 0.79 – 0.76 0.76 0.91 0.89 0.94 0.64 0.73 0.65 – – 0.43 0.71 0.45 0.49
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 – – 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria – – 0 0 0.06 0.13 0.17 0.17 0.29 – 0.36 0.32 0 0 0 0 0 0 – – 0 0 0 0
Nucleus – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 13 2 3 6 2 3 10 – 6 1 10 9 5 34 17 34 – – 22 4 12 16
Bud – – 9 9 9 15 11 9 21 – 24 19 11 6 11 6 2 7 – – 2 0 1 2
Bud Neck – – 11 0 1 0 1 0 0 – 0 0 0 1 3 0 1 1 – – 38 14 22 31
Bud Site – – 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 121 122 139 104 115 91 80 – 74 100 197 244 226 504 239 379 – – 160 33 94 112
Cytoplasm – – 417 367 481 495 534 767 430 – 384 422 326 365 402 543 375 477 – – 202 160 127 181
Endoplasmic Reticulum – – 4 1 0 5 4 2 6 – 3 4 1 4 3 41 19 25 – – 15 2 10 11
Endosome – – 0 0 0 0 0 0 0 – 0 1 0 0 0 1 2 0 – – 1 0 1 2
Golgi – – 1 0 0 0 0 0 0 – 0 0 0 0 0 2 0 1 – – 1 0 0 1
Mitochondria – – 2 17 33 70 102 142 161 – 182 176 0 0 1 20 1 9 – – 19 1 3 4
Nucleus – – 0 1 0 1 0 3 3 – 0 1 0 0 2 16 10 15 – – 0 0 0 0
Nuclear Periphery – – 0 1 0 0 0 2 0 – 0 2 1 0 3 0 0 0 – – 0 0 1 0
Nucleolus – – 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole – – 1 0 1 0 1 0 0 – 1 1 0 0 0 6 2 8 – – 0 0 0 0
Vac/Vac Membrane – – 0 0 5 2 6 5 0 – 5 0 0 1 0 32 13 27 – – 3 3 3 4
Unique Cell Count 454 379 530 552 613 850 547 507 554 358 408 429 854 512 729 474 226 282 373
Labelled Cell Count 579 520 672 698 777 1025 711 679 728 546 630 656 1205 681 983 474 226 282 373


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 5.2 5.0 5.4 5.0 4.8 4.9 4.3 – 4.2 4.2 6.6 6.8 5.9 9.4 9.6 9.5 6.3 6.6 7.4
Std Deviation (1e-4) – – 0.7 1.0 1.2 1.1 0.9 0.8 0.7 – 0.7 0.7 0.9 0.9 0.8 1.2 1.4 1.5 1.1 1.2 1.2
Intensity Change (Log2) – – – -0.05 0.05 -0.07 -0.11 -0.09 -0.28 – -0.3 -0.32 0.34 0.39 0.17 0.85 0.88 0.86 0.28 0.34 0.5

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.5 -2.8 -2.1 0 -3.9 0 – 0 0 -0.1 -0.6 -1.8 1.0 0.4 1.5
Bud 0.4 -0.3 0.8 0 -1.4 0 – 0 0 1.0 -0.6 0.6 -2.1 -2.3 -1.5
Bud Neck -3.1 -3.2 0 0 0 0 – 0 0 0 -2.7 -2.0 0 -3.1 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 1.8 -0.2 -3.0 0 -7.4 0 – 0 0 8.2 9.8 7.9 11.2 6.4 8.6
Cytoplasm 3.0 -0.6 -1.2 -2.5 -1.0 -5.8 – -6.7 -6.6 -0.4 -1.2 1.1 -11.0 -7.5 -10.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.7 2.9 2.8
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.9 4.9 7.5 0 8.9 0 – 0 0 0 0 0 2.5 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 2.9 0 3.1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 4.2 3.4 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8915 4.8872 4.0694 3.679 3.3801 4.048 1.1965 2.3336 1.9486 1.5183 1.6356 1.9086 1.8144 2.5145 1.8185 2.7123 2.2965 2.0943
Actin 0.0351 0.0009 0.0129 0.0157 0.0029 0.0032 0.0198 0.0023 0.0213 0.0539 0.0372 0.032 0.0524 0.001 0.0318 0.0486 0.0333 0.0128
Bud 0.0007 0.0001 0.0008 0.0008 0.0009 0.0005 0.0029 0.0004 0.0048 0.0005 0.003 0.0016 0.0044 0.0047 0.0116 0.0019 0.0299 0.0016
Bud Neck 0.0152 0.0015 0.0146 0.0094 0.0185 0.0175 0.0115 0.0048 0.0224 0.0142 0.0525 0.041 0.0052 0.0019 0.0103 0.0051 0.0206 0.0076
Bud Periphery 0.0031 0.0008 0.0051 0.0056 0.0083 0.0036 0.0088 0.0014 0.0106 0.0031 0.0053 0.0107 0.0065 0.0059 0.0139 0.0052 0.0297 0.0051
Bud Site 0.0166 0.031 0.0247 0.003 0.0045 0.0048 0.0148 0.1206 0.0646 0.0047 0.048 0.0024 0.0163 0.0908 0.0626 0.0322 0.0817 0.0013
Cell Periphery 0.1052 0.0786 0.1201 0.0869 0.1337 0.0678 0.158 0.1235 0.1383 0.1905 0.0571 0.0931 0.0637 0.0488 0.0706 0.0525 0.0345 0.0291
Cytoplasm 0.596 0.7762 0.6325 0.7026 0.687 0.815 0.6509 0.715 0.6631 0.6323 0.6982 0.7723 0.5419 0.766 0.6422 0.7079 0.4833 0.8301
Cytoplasmic Foci 0.0208 0.0006 0.004 0.0017 0.0016 0.0013 0.037 0.0007 0.0044 0.0036 0.024 0.0163 0.0231 0.0011 0.0059 0.015 0.0136 0.0041
Eisosomes 0.0021 0.0004 0.0023 0.0009 0.0007 0.0002 0.0005 0.0005 0.0011 0.0005 0.0005 0.0004 0.0011 0.0005 0.0011 0.0003 0.0007 0.0002
Endoplasmic Reticulum 0.0099 0.0017 0.003 0.0054 0.0086 0.0023 0.0047 0.0022 0.0061 0.0036 0.0102 0.0039 0.0126 0.0022 0.0071 0.0034 0.0069 0.0041
Endosome 0.011 0.0001 0.0009 0.0003 0.0036 0.0005 0.0112 0.0001 0.0042 0.0006 0.0067 0.0018 0.0314 0.0001 0.0065 0.0077 0.0526 0.0039
Golgi 0.008 0.0001 0.0013 0.0004 0.0004 0.0006 0.0053 0.0001 0.0049 0.0026 0.0055 0.0018 0.0123 0.0001 0.0043 0.0093 0.0229 0.005
Lipid Particles 0.0085 0.0003 0.003 0.001 0.0021 0.0019 0.0076 0.0003 0.0037 0.0019 0.002 0.0016 0.0209 0.0005 0.0062 0.004 0.0339 0.0017
Mitochondria 0.0032 0.0001 0.0019 0.0002 0.0004 0.0002 0.0031 0 0.0024 0.0022 0.0026 0.0005 0.0121 0 0.0236 0.0022 0.0126 0.0037
None 0.1184 0.106 0.1601 0.1627 0.0847 0.0759 0.024 0.0262 0.0398 0.0781 0.0167 0.0167 0.1391 0.0739 0.07 0.0901 0.1109 0.0826
Nuclear Periphery 0.0071 0.0001 0.0002 0.0004 0.0109 0.0002 0.0043 0.0001 0.0006 0.0001 0.0039 0.0004 0.0131 0.0002 0.0051 0.0008 0.0035 0.001
Nucleolus 0.0022 0.0001 0.0003 0.0002 0.0013 0.0001 0.0025 0.0001 0.0002 0.0001 0.0007 0.0002 0.0025 0.0002 0.0032 0.0013 0.0039 0.0002
Nucleus 0.0081 0.0003 0.0005 0.0008 0.0205 0.0003 0.0043 0.0004 0.0008 0.0003 0.0157 0.0004 0.0138 0.0005 0.0083 0.0014 0.0049 0.0009
Peroxisomes 0.0129 0 0.0092 0.0001 0.0001 0.0029 0.0113 0 0.0038 0.0056 0.0046 0.0007 0.0089 0 0.0032 0.0039 0.0059 0.0006
Punctate Nuclear 0.0105 0 0.0004 0.0004 0.0033 0.0003 0.0125 0.0001 0.0006 0.0001 0.0014 0.0011 0.0137 0.0003 0.0049 0.0041 0.0063 0.0009
Vacuole 0.0044 0.0011 0.0019 0.0014 0.0056 0.0009 0.0042 0.0011 0.0023 0.0012 0.0036 0.001 0.0041 0.0013 0.0062 0.003 0.0068 0.0034
Vacuole Periphery 0.0006 0 0.0001 0 0.0004 0 0.0006 0 0.0002 0 0.0007 0.0001 0.0011 0 0.0015 0.0002 0.0015 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3965 13.2459 12.2317 16.936 11.7403 17.442 16.9643 23.2009 20.866 18.4688
Translational Efficiency 0.6889 0.3838 0.4872 0.3634 0.3361 0.3929 0.4295 0.3122 0.416 0.3414

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1894 401 572 1192 1924 1881 1567 2771 3818 2282 2139 3963

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 743.32 856.53 1249.18 984.03 754.07 817.49 963.02 1006.85 748.74 824.35 1039.54 999.99
Standard Deviation 84.16 109.82 147.28 133.23 103.74 105.11 138.62 126.74 94.69 106.99 189.52 129.15
Intensity Change Log 2 — 0.204520 0.748926 0.404719 — 0.116503 0.352867 0.417078 — 0.160867 0.563032 0.410956

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003401 0.004845 0.012391 0.013926 0.004056 0.005148 0.005654 0.010893 0.003731 0.005095 0.007456 0.011805
Bud Neck 0.092526 0.055631 0.014252 0.012924 0.052561 0.054516 0.012372 0.016999 0.072387 0.054712 0.012875 0.015773
Bud Site 0.047506 0.069111 0.097963 0.073105 0.041295 0.041434 0.043131 0.066197 0.044376 0.046297 0.057794 0.068275
Cell Periphery 0.015951 0.001827 0.009718 0.003712 0.004506 0.001911 0.008304 0.002848 0.010183 0.001896 0.008682 0.003108
Cytoplasm 0.619373 0.474929 0.415698 0.469825 0.583997 0.546098 0.532874 0.653707 0.601546 0.533592 0.501539 0.598399
Cytoplasmic Foci 0.043400 0.159097 0.082939 0.042228 0.105595 0.102176 0.042323 0.014181 0.074742 0.112179 0.053184 0.022617
Eisosomes 0.020618 0.002223 0.001408 0.001933 0.007514 0.004042 0.004278 0.001838 0.014015 0.003723 0.003510 0.001866
Endoplasmic Reticulum 0.002421 0.001788 0.010301 0.002997 0.002221 0.001577 0.009393 0.000668 0.002320 0.001614 0.009636 0.001369
Endosome 0.005993 0.019270 0.004059 0.009923 0.010545 0.015535 0.003209 0.002377 0.008287 0.016191 0.003436 0.004647
Golgi 0.007597 0.005697 0.000828 0.003932 0.007527 0.007183 0.000525 0.000968 0.007562 0.006922 0.000606 0.001860
Lipid Particles 0.007874 0.004644 0.009864 0.002549 0.007990 0.006750 0.003760 0.001263 0.007932 0.006380 0.005393 0.001650
Mitochondria 0.002208 0.000516 0.000060 0.004205 0.002696 0.003737 0.000705 0.002214 0.002454 0.003171 0.000533 0.002813
Mitotic Spindle 0.000300 0.000281 0.000696 0.009758 0.000397 0.003278 0.000913 0.010644 0.000348 0.002751 0.000855 0.010377
None 0.004812 0.000503 0.000520 0.000620 0.004450 0.002817 0.004171 0.000790 0.004630 0.002410 0.003195 0.000739
Nuclear Periphery 0.000091 0.000055 0.000164 0.000220 0.000107 0.000155 0.000153 0.000126 0.000099 0.000137 0.000156 0.000154
Nuclear Periphery Foci 0.000909 0.001153 0.014121 0.001989 0.000969 0.000586 0.002591 0.000355 0.000940 0.000686 0.005675 0.000846
Nucleolus 0.000464 0.000158 0.000057 0.000151 0.000608 0.000606 0.000152 0.000085 0.000536 0.000527 0.000127 0.000105
Nucleus 0.038400 0.023001 0.052832 0.035267 0.068203 0.057307 0.091178 0.048058 0.053418 0.051279 0.080924 0.044211
Peroxisomes 0.003072 0.007995 0.000881 0.003650 0.003836 0.007560 0.001101 0.001944 0.003457 0.007636 0.001042 0.002457
Vacuole 0.082336 0.166682 0.271151 0.305151 0.090260 0.136521 0.232815 0.163269 0.086329 0.141821 0.243067 0.205944
Vacuole Periphery 0.000749 0.000593 0.000096 0.001935 0.000668 0.001063 0.000396 0.000576 0.000708 0.000980 0.000316 0.000985

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.65 -8.64 -5.16 -3.65 0.62 -1.53 -2.30 -5.11 -3.53 -3.60 -2.39 -7.17 -7.38 -4.79 -2.80
Bud Neck 6.72 27.49 28.58 8.74 3.28 -0.59 19.09 18.04 18.74 -2.21 7.01 33.04 32.70 21.03 -0.63
Bud Site -3.66 -8.77 -3.11 1.30 5.34 0.01 0.21 -4.28 -4.01 -3.87 -0.67 -3.86 -4.78 -3.47 -0.44
Cell Periphery 25.64 8.15 22.27 -2.37 6.23 10.11 -3.95 8.39 -1.40 7.38 26.56 4.27 24.01 -2.49 9.26
Cytoplasm 11.78 20.59 19.04 2.12 -4.26 5.41 9.23 -4.64 -9.25 -12.54 11.62 19.06 6.91 -4.60 -11.87
Cytoplasmic Foci -15.91 -10.15 1.70 16.24 10.68 0.89 20.68 31.59 29.70 14.54 -11.96 10.46 28.41 32.09 16.88
Eisosomes 22.67 24.56 23.91 2.81 -0.83 8.21 5.34 17.04 9.29 3.74 21.16 17.37 28.12 9.72 3.61
Endoplasmic Reticulum 1.26 -6.21 0.30 -0.77 5.94 1.88 -4.39 6.87 3.31 5.53 2.57 -5.87 5.69 1.64 6.95
Endosome -4.85 5.19 -0.71 4.19 -2.58 -4.45 11.36 12.84 14.07 2.95 -8.15 12.56 9.21 12.99 -0.40
Golgi 3.15 23.65 5.87 3.00 -2.11 0.67 20.16 18.20 12.99 -1.31 1.56 31.17 18.15 11.56 -2.49
Lipid Particles 3.77 -2.16 15.43 2.64 11.00 1.89 6.52 12.65 12.09 4.82 3.26 5.93 19.71 11.95 8.97
Mitochondria 5.60 7.23 -0.42 -2.28 -2.77 -1.20 3.40 1.69 2.68 -1.89 -1.10 5.12 1.11 1.81 -3.09
Mitotic Spindle 0.03 -0.61 -4.00 -4.02 -3.77 -3.49 -1.37 -6.01 -3.14 -5.38 -3.54 -1.58 -7.21 -4.01 -6.50
None 6.06 6.22 6.08 -0.12 -0.39 1.89 0.77 7.19 3.20 3.11 3.12 2.27 9.10 3.22 3.09
Nuclear Periphery 4.73 -10.54 -3.51 -4.48 -0.53 -1.66 -3.97 -0.79 0.42 0.77 -1.74 -8.62 -2.14 -0.58 0.48
Nuclear Periphery Foci -0.89 -17.58 -6.29 -2.43 15.94 4.76 -8.48 8.43 2.82 11.99 3.53 -17.76 1.50 -2.12 17.98
Nucleolus 12.07 25.19 10.96 0.86 -2.87 -0.07 9.33 11.94 6.46 2.19 0.00 14.00 17.37 6.39 1.04
Nucleus 7.73 -5.43 2.14 -4.89 6.29 3.94 -4.64 9.01 4.04 11.20 1.07 -8.94 6.51 3.84 12.81
Peroxisomes -6.40 9.23 0.60 5.36 -2.95 -5.78 6.60 7.72 8.86 -1.28 -7.69 8.05 5.52 9.63 -2.57
Vacuole -10.09 -23.22 -34.63 -16.65 -4.41 -10.92 -26.90 -27.39 -15.18 5.97 -15.35 -35.37 -42.27 -23.46 2.11
Vacuole Periphery 0.74 3.14 -1.02 -1.53 -2.77 -2.08 2.01 1.57 2.65 -0.30 -1.42 2.76 0.29 1.42 -1.86
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Duf1

Duf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Duf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available