Standard name
Human Ortholog
Description Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.07 0.05 0 – 0 0 0.06 0.13 0.13 0.08 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.07 0.1 0 0.15 0.13 0.2 0.24 – 0.28 0.31 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.97 0.96 0.97 0.94 0.91 0.81 0.79 0.82 – 0.84 0.79 0.91 0.82 0.78 0.9 0.93 0.91 0.94 0.95 0.93 0.91 0.78 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.14 0.12 0.13 0.23 – 0.2 0.23 0 0 0 0 0 0 0 0 0 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 0 0 1 2 3 3 – 3 4 0 0 2 0 0 0 2 0 1 2 3 3
Bud Neck 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 0 6 8 17 9 – 20 18 0 3 8 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 1 3 3 13 3 27 26 7 – 4 10 21 56 59 23 12 17 0 0 0 2 2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 4 6 0 0 1 0 0 1
Endosome 1 0 0 0 1 0 0 1 1 – 0 0 1 3 3 0 2 1 1 1 2 1 1 5
Golgi 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Mitochondria 2 7 16 25 16 50 54 114 104 – 89 129 12 1 24 3 1 7 2 0 0 0 2 13
Nucleus 211 116 209 235 336 301 329 455 347 – 271 323 310 354 367 263 247 248 203 115 210 210 274 232
Nuclear Periphery 1 0 0 2 4 3 11 7 7 – 9 8 3 2 9 3 0 1 0 0 0 0 0 3
Nucleolus 1 2 1 5 8 46 49 75 96 – 65 93 4 12 21 5 5 8 1 0 0 1 8 34
Peroxisomes 0 0 0 0 1 0 0 0 0 – 1 0 0 3 9 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 1 0 0 1 0 0 – 1 0 2 11 10 0 1 1 0 1 0 0 2 3
Vac/Vac Membrane 1 0 2 0 2 0 1 2 1 – 2 1 3 8 6 10 4 4 2 1 8 8 49 86
Unique Cell Count 218 120 218 243 357 331 407 576 425 323 410 340 432 468 293 266 274 217 122 227 233 350 394
Labelled Cell Count 221 126 231 274 381 410 482 701 575 465 586 356 453 518 309 276 293 217 122 227 233 350 394


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 6.9 6.2 6.0 6.6 6.2 6.1 5.7 5.5 – 5.3 5.0 7.2 7.8 6.7 9.1 11.0 10.6 7.2 6.8 7.3
Std Deviation (1e-4) 2.0 1.6 1.4 1.4 1.7 1.6 1.7 1.5 1.5 – 1.5 1.5 2.7 2.6 2.3 3.0 4.0 3.5 1.5 1.6 1.7
Intensity Change (Log2) – – – -0.05 0.1 0.01 -0.02 -0.11 -0.18 – -0.22 -0.31 0.23 0.33 0.11 0.55 0.82 0.77 0.22 0.13 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 3.7 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 1.6 0 2.9 2.1 0 – 0 0 2.7 4.9 4.8 3.3 2.0 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.1 -1.5 2.7 2.2 4.2 5.3 – 5.8 6.8 -2.0 -5.4 -1.2 -3.7 -4.1 -2.5
Nucleus 0.5 -0.9 -2.2 -5.2 -5.7 -5.0 – -4.3 -5.7 -2.1 -4.9 -5.8 -2.6 -1.4 -2.3
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 5.5 5.1 5.4 7.4 – 6.9 7.4 0 0 2.8 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 1.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6353 7.0554 7.2848 5.4484 5.0948 6.0311 5.363 7.4733 7.198 5.6129 5.6536 5.7744 6.5164 7.8137 7.5061 7.9659 7.2373 6.8529
Actin 0.0103 0 0.0011 0.0162 0.0235 0 0.0151 0 0.0168 0.0148 0.0052 0.0016 0 0 0 0 0 0.0001
Bud 0.0003 0 0.0001 0.0004 0.0003 0 0.0003 0 0.0002 0.0003 0.0011 0.0001 0 0 0 0 0 0
Bud Neck 0.0008 0 0.004 0.0036 0.0011 0.0003 0.001 0 0.0002 0.001 0.0005 0.0003 0 0 0 0 0.0001 0.0018
Bud Periphery 0.0005 0 0.0001 0.0007 0.0005 0 0.0005 0 0.0004 0.0003 0.0028 0.0004 0 0 0 0 0.0001 0.0001
Bud Site 0.0048 0 0.004 0.0028 0.0015 0 0.0014 0 0.0005 0.0034 0.0031 0.0002 0 0 0 0 0.0001 0.0001
Cell Periphery 0.0002 0 0.0002 0.0005 0.0003 0 0.0002 0 0.0001 0.0001 0.0003 0.0001 0 0 0 0 0 0
Cytoplasm 0.0067 0 0.0002 0.0027 0.0014 0 0.0101 0 0.0011 0.0051 0.0019 0.0001 0.0001 0 0.0002 0.0001 0.0001 0
Cytoplasmic Foci 0.0025 0 0.0006 0.0031 0.0042 0 0.0078 0 0.0013 0.0462 0.0266 0.0014 0 0 0 0 0 0
Eisosomes 0.0002 0 0 0.0006 0.0004 0 0.0001 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0011 0 0.0001 0.0022 0.0021 0 0.0041 0 0.0048 0.0013 0.0015 0.0005 0 0 0 0 0 0
Endosome 0.0032 0 0.0017 0.0056 0.0093 0 0.0126 0 0.0042 0.0262 0.0258 0.0016 0 0 0 0 0 0
Golgi 0.0006 0 0.0007 0.0021 0.0035 0 0.0017 0 0.0025 0.0089 0.0093 0.0005 0 0 0 0 0 0
Lipid Particles 0.0011 0 0.0017 0.0135 0.0082 0 0.0054 0 0.0105 0.0192 0.0681 0.0043 0 0 0 0 0 0
Mitochondria 0.0012 0.0001 0.0004 0.0062 0.0033 0.0001 0.0018 0.0001 0.0042 0.0149 0.0253 0.0032 0.0001 0.0001 0.0001 0.0001 0.0008 0.0001
None 0.0071 0 0.0001 0.0035 0.0032 0 0.0045 0 0.0003 0.0031 0.0016 0.0002 0 0 0.0001 0 0 0
Nuclear Periphery 0.0053 0.0012 0.0006 0.0113 0.007 0.0001 0.0256 0.0025 0.0039 0.006 0.0295 0.01 0.0006 0.0007 0.0014 0.001 0.0022 0.0005
Nucleolus 0.0176 0.0133 0.0099 0.0178 0.0622 0.0376 0.0139 0.006 0.006 0.0105 0.0486 0.0204 0.0288 0.0136 0.0083 0.0023 0.059 0.0421
Nucleus 0.9294 0.9843 0.9728 0.8776 0.8517 0.9617 0.8695 0.9912 0.9387 0.811 0.66 0.9529 0.9663 0.9804 0.9783 0.9965 0.9101 0.9546
Peroxisomes 0.001 0 0.0006 0.0203 0.0021 0 0.0018 0 0.0019 0.0103 0.0162 0.0002 0 0 0 0 0 0
Punctate Nuclear 0.0043 0.001 0.0007 0.0063 0.0123 0.0001 0.0186 0 0.0006 0.0127 0.0578 0.0007 0.0038 0.005 0.0115 0 0.0271 0.0005
Vacuole 0.0013 0 0.0002 0.0021 0.0011 0.0001 0.0025 0 0.001 0.0035 0.0087 0.0005 0 0 0 0 0.0001 0.0001
Vacuole Periphery 0.0005 0 0.0001 0.0008 0.0009 0 0.0015 0 0.0007 0.0009 0.0061 0.0008 0 0 0 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 96.4288 35.0939 17.9507 32.8896 69.6775 75.8052 25.579 21.5075 26.7014 75.8256
Translational Efficiency 1.1256 0.9141 0.8006 0.837 0.9589 1.4161 1.0971 0.6644 0.8201 0.8908

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
928 583 1850 1136 899 1944 198 108 1827 2527 2048 1244

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 713.89 902.28 1066.36 1127.68 763.99 907.25 1238.84 1264.13 738.54 906.10 1083.04 1139.53
Standard Deviation 97.87 181.60 157.15 188.88 134.17 136.76 164.33 211.39 119.79 148.33 165.89 194.76
Intensity Change Log 2 — 0.337873 0.578921 0.659584 — 0.247946 0.697364 0.726519 — 0.292086 0.641363 0.694574

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000100 0.000492 0.000470 0.001237 0.000327 0.000308 0.000934 0.000880 0.000212 0.000350 0.000515 0.001206
Bud Neck 0.000860 0.003012 0.013248 0.022077 0.002472 0.005481 0.007807 0.012629 0.001653 0.004911 0.012722 0.021256
Bud Site 0.000197 0.000448 0.000940 0.008718 0.001642 0.000711 0.000689 0.002522 0.000908 0.000650 0.000916 0.008180
Cell Periphery 0.000126 0.000167 0.000095 0.000229 0.000229 0.000150 0.000032 0.000044 0.000176 0.000154 0.000089 0.000213
Cytoplasm 0.004954 0.000794 0.000360 0.002385 0.004573 0.001093 0.000122 0.000221 0.004766 0.001024 0.000337 0.002197
Cytoplasmic Foci 0.000549 0.000138 0.000015 0.004622 0.000517 0.000580 0.000016 0.002008 0.000533 0.000478 0.000015 0.004395
Eisosomes 0.000011 0.000027 0.000041 0.000035 0.000021 0.000029 0.000056 0.000041 0.000016 0.000029 0.000042 0.000036
Endoplasmic Reticulum 0.002790 0.007010 0.003397 0.003526 0.006156 0.006384 0.004186 0.003646 0.004446 0.006529 0.003474 0.003536
Endosome 0.000222 0.000223 0.000142 0.005504 0.000325 0.000677 0.000063 0.006052 0.000273 0.000572 0.000135 0.005552
Golgi 0.000074 0.000190 0.000027 0.003786 0.000069 0.000787 0.000040 0.002086 0.000071 0.000649 0.000028 0.003638
Lipid Particles 0.000253 0.000931 0.000038 0.000979 0.000114 0.000154 0.000051 0.000363 0.000185 0.000333 0.000039 0.000926
Mitochondria 0.000611 0.000940 0.001009 0.008525 0.000723 0.002020 0.001188 0.001949 0.000666 0.001771 0.001026 0.007954
Mitotic Spindle 0.000621 0.001013 0.000298 0.016592 0.000315 0.003758 0.000036 0.001085 0.000470 0.003125 0.000272 0.015246
None 0.006301 0.004005 0.000813 0.003463 0.008215 0.001894 0.000426 0.000698 0.007243 0.002381 0.000776 0.003223
Nuclear Periphery 0.000202 0.000169 0.000281 0.002689 0.000257 0.000447 0.000136 0.000764 0.000229 0.000383 0.000267 0.002522
Nuclear Periphery Foci 0.000061 0.000578 0.000140 0.002242 0.000353 0.000212 0.000015 0.003888 0.000205 0.000296 0.000128 0.002385
Nucleolus 0.004180 0.006753 0.004377 0.007887 0.009908 0.005649 0.004762 0.016358 0.006999 0.005903 0.004415 0.008622
Nucleus 0.977165* 0.971972* 0.973267* 0.896318* 0.962738* 0.967509* 0.977801* 0.934391* 0.970066* 0.968539* 0.973705* 0.899624*
Peroxisomes 0.000297 0.000376 0.000161 0.001420 0.000305 0.000316 0.000484 0.000433 0.000301 0.000330 0.000192 0.001335
Vacuole 0.000364 0.000622 0.000706 0.005773 0.000511 0.001143 0.001075 0.007066 0.000436 0.001023 0.000742 0.005885
Vacuole Periphery 0.000063 0.000139 0.000174 0.001993 0.000230 0.000698 0.000079 0.002876 0.000145 0.000569 0.000165 0.002069

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.15 -14.12 -10.98 -6.84 -7.24 0.14 -10.51 -5.43 -5.36 -1.26 -3.68 -11.58 -10.67 -8.77 -7.29
Bud Neck -5.75 -19.08 -16.47 -14.62 -6.98 -4.51 -5.11 -4.44 -3.23 -2.11 -6.65 -18.27 -16.41 -13.42 -7.26
Bud Site -3.50 -6.43 -4.65 -4.48 -4.08 1.21 1.14 -0.77 -1.73 -1.69 0.59 -0.30 -4.14 -4.42 -4.18
Cell Periphery -1.11 1.48 -1.10 -0.02 -1.90 1.75 4.69 4.14 4.44 -1.45 0.83 3.48 0.47 -0.12 -1.96
Cytoplasm 4.17 4.71 1.22 -3.29 -3.95 2.57 3.51 3.35 1.79 -3.16 4.27 5.51 1.54 -2.68 -3.98
Cytoplasmic Foci 2.17 2.98 -2.62 -3.25 -3.43 0.36 1.52 -0.30 -0.51 -1.07 0.63 2.48 -2.42 -2.80 -3.53
Eisosomes -6.98 -17.05 -14.21 -3.94 1.79 -3.66 -10.63 -5.03 -3.23 2.47 -7.75 -16.45 -12.70 -4.48 2.41
Endoplasmic Reticulum -5.60 -2.33 -2.87 4.38 -1.28 -0.49 3.56 0.99 1.16 -0.19 -5.93 3.46 1.79 7.66 -1.17
Endosome 0.03 0.77 -4.38 -4.41 -4.53 -1.25 2.42 -1.15 -1.03 -1.25 -1.41 1.76 -4.45 -3.88 -4.67
Golgi -1.07 0.83 -3.81 -3.56 -3.90 -1.75 1.23 -1.09 -0.41 -1.12 -1.84 1.53 -3.94 -2.46 -4.04
Lipid Particles -0.79 1.45 -1.33 0.35 -2.50 -0.66 2.28 -1.09 -0.83 -1.57 -0.69 1.92 -1.77 -0.89 -2.58
Mitochondria -0.67 -1.03 -4.23 -4.03 -4.03 -2.12 -3.33 -4.30 -0.31 -3.00 -2.18 -1.51 -4.42 -3.28 -4.10
Mitotic Spindle -0.44 0.25 -5.29 -5.04 -5.44 -3.04 1.06 -1.30 2.23 -2.01 -2.90 0.13 -5.44 -4.06 -5.47
None 1.85 6.56 3.09 0.58 -5.35 4.32 5.48 5.09 2.08 -2.79 5.44 7.86 4.48 -1.38 -5.45
Nuclear Periphery 0.91 -1.25 -2.28 -2.32 -2.15 -2.40 2.93 -2.60 -1.63 -3.10 -2.60 -0.81 -2.31 -2.10 -2.23
Nuclear Periphery Foci -1.40 -0.76 -3.21 -1.63 -2.98 0.74 2.19 -0.90 -0.96 -1.06 -0.82 0.70 -3.00 -2.75 -3.16
Nucleolus -2.18 -0.82 -4.91 -3.01 -4.79 3.85 4.51 -0.72 -1.44 -1.57 1.39 2.87 -3.36 -4.24 -5.04
Nucleus 1.34 2.11 11.28 10.07 10.88 -1.20 -3.94 1.44 1.79 2.44 0.52 -0.92 10.42 10.14 11.15
Peroxisomes -0.35 0.69 -1.58 -1.50 -2.03 -0.04 -1.81 -0.97 -0.84 0.44 -0.24 1.11 -1.66 -1.61 -2.01
Vacuole -1.76 -1.66 -5.63 -5.25 -5.10 -2.63 -4.73 -2.26 -1.99 -1.96 -3.19 -1.74 -5.95 -5.11 -5.42
Vacuole Periphery -2.04 -1.16 -4.03 -3.75 -3.51 -1.36 1.34 -1.19 -0.85 -1.29 -1.64 -0.18 -3.87 -1.92 -3.74
Standard name
Human Ortholog
Description Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Msh2

Msh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available