Standard name
Human Ortholog
Description tRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.06 0 0 0.05 0.05 0 0.06 0.05 0.05 0.11 0.1 0.14 0.12 0 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.08 0.05 0.09 0.34 0.24 0.29 0.28 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.9 0.92 0.95 0.93 0.89 0.87 0.89 0.84 0.81 0.81 0.79 0.84 0.86 0.84 0.83 0.78 0.88 0.83 0.9 0.9 0.94 0.86 0.83 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.08 0.06 0 0.06 0 0 0 0 0.05 0 0 0.07 0.11 0.12 0 0 0 0.06 0.12 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 1 2 4 2 2 0 1 0 0 0 0 0 2 1 2 0 1
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 2 0 3 14 6 6 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 11 10 12 7 10 25 27 13 22 12 15 4 25 32 5 2 8 0 0 0 0 1 3
Endoplasmic Reticulum 0 1 0 0 0 0 4 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0
Endosome 0 0 1 1 1 1 3 1 0 2 1 2 0 4 0 0 1 1 0 3 1 0 0 1
Golgi 2 1 0 0 0 0 1 0 0 0 0 0 1 0 1 1 1 0 2 1 0 0 0 2
Mitochondria 1 13 1 0 0 26 25 44 116 81 69 80 0 1 0 3 0 0 0 5 1 1 0 2
Nucleus 26 224 293 177 175 279 444 419 277 277 187 238 32 204 190 31 49 71 74 222 284 57 156 147
Nuclear Periphery 0 0 4 0 3 1 4 1 6 5 1 5 0 3 1 1 0 1 0 0 0 0 0 0
Nucleolus 0 1 0 0 1 4 4 7 9 7 17 10 0 4 2 0 0 0 0 0 0 0 3 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 1 8 3 1 3 4 0 0 1 0 0 1 0 0 0 0 1 2
Vac/Vac Membrane 0 11 9 7 15 20 16 31 11 10 6 11 2 8 7 3 6 10 2 7 8 3 23 44
Unique Cell Count 29 244 308 191 196 321 500 496 342 343 236 283 37 242 228 40 56 86 83 247 304 67 188 214
Labelled Cell Count 30 262 318 197 204 346 529 543 451 416 304 381 39 251 234 44 59 92 83 247 304 67 188 214


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 6.3 8.7 7.9 8.3 5.9 6.1 6.0 4.9 5.2 4.8 5.0 9.0 8.3 7.5 6.6 8.5 8.7 8.3 8.7 8.8
Std Deviation (1e-4) 1.1 1.1 1.3 1.4 3.0 1.0 1.9 1.4 1.4 1.6 1.2 1.2 1.9 1.5 1.6 1.5 1.7 1.9 2.0 1.3 1.8
Intensity Change (Log2) – – – -0.15 -0.07 -0.57 -0.51 -0.55 -0.83 -0.74 -0.85 -0.79 0.04 -0.07 -0.21 -0.4 -0.04 -0.0 -0.08 -0.01 0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.6 0 0 4.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 0.2 -0.1 1.2 1.4 0.4 1.9 1.1 1.2 0 3.4 4.6 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.8 3.7 5.1 11.1 8.9 0 9.9 0 0 0 0 0 0
Nucleus -1.1 -2.5 -3.6 -3.1 -4.6 -5.5 -5.5 -5.7 -4.4 -2.1 -4.3 -4.5 -4.1 -2.2 -3.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.5 2.4 2.0 0.2 2.1 0.2 0 -0.3 0.6 0 0.3 0.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5566 6.478 6.0719 5.4454 5.2499 5.9246 3.0173 3.4858 3.0065 3.0061 1.9926 2.8239 1.2647 2.0792 1.7178 1.7618 0.9109 1.5266
Actin 0.0318 0 0.0102 0.0146 0.0002 0.0013 0.0585 0.0005 0.002 0 0.0009 0.0017 0.01 0 0 0 0.0002 0
Bud 0.0009 0 0.0001 0.0005 0 0.0001 0.0007 0.0002 0.0002 0 0.0001 0 0.0002 0 0 0 0.0002 0.0001
Bud Neck 0.0039 0.0002 0.0005 0.0005 0.0006 0.0011 0.0019 0.0003 0.0004 0.0001 0.0001 0.0009 0.0007 0.0001 0 0 0.0004 0.0018
Bud Periphery 0.003 0.0001 0.0002 0.0012 0.0001 0.0002 0.0019 0.0011 0.0004 0 0.0002 0.0001 0.0002 0 0 0 0.0002 0.0001
Bud Site 0.0088 0.0002 0.0006 0.002 0.0001 0.0005 0.0022 0.0004 0.0013 0 0.0002 0.0001 0.0004 0 0 0 0.0002 0.0001
Cell Periphery 0.0007 0 0.0001 0.0002 0 0 0.0009 0.0004 0.0001 0 0 0 0.0001 0 0 0 0 0.0001
Cytoplasm 0.009 0.002 0.0018 0.0002 0.0106 0.0003 0.0064 0.0006 0.0054 0.0038 0.0006 0.0026 0.0037 0.0008 0.0023 0.0003 0.0012 0.0003
Cytoplasmic Foci 0.0104 0 0.0012 0.0005 0.0002 0.0006 0.023 0 0.0017 0 0.0001 0.0019 0.0032 0 0 0 0.0001 0
Eisosomes 0.0007 0 0.0001 0.0006 0 0 0.0009 0 0.0003 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0024 0.0001 0.0002 0.0002 0.0005 0.0001 0.0119 0.0002 0.0012 0.0001 0.0001 0.0002 0.0008 0.0001 0.0002 0 0.0004 0
Endosome 0.0082 0.0001 0.0022 0.0022 0.0005 0.0002 0.034 0.0007 0.0039 0 0.0009 0.0019 0.0047 0 0 0 0.0005 0
Golgi 0.0044 0 0.0025 0.0028 0.0001 0.0002 0.0154 0.0002 0.0011 0 0.0005 0.001 0.0014 0 0 0 0 0
Lipid Particles 0.012 0 0.0024 0.0028 0.0003 0.0004 0.054 0.0004 0.0039 0 0.0009 0.0036 0.0019 0 0 0 0 0
Mitochondria 0.0233 0.0002 0.0109 0.0509 0.0004 0.0009 0.0281 0.0053 0.0093 0.0002 0.0663 0.0015 0.0016 0.0001 0.0001 0.0002 0.0007 0.0001
None 0.0176 0.0017 0.0038 0.0002 0.0134 0.0002 0.0062 0.0002 0.0133 0.0078 0.0002 0.0011 0.0013 0 0.0001 0 0.0001 0
Nuclear Periphery 0.0124 0.003 0.0022 0.0017 0.0162 0.006 0.0662 0.0074 0.0069 0.0067 0.0021 0.0019 0.0076 0.0045 0.0024 0.0016 0.0083 0.0004
Nucleolus 0.0114 0.009 0.008 0.0032 0.0301 0.0161 0.0072 0.0115 0.0037 0.0034 0.0049 0.0057 0.0075 0.0021 0.0023 0.0045 0.0067 0.0078
Nucleus 0.8007 0.9811 0.9491 0.9123 0.8536 0.9612 0.5957 0.9651 0.929 0.9763 0.9193 0.9649 0.9467 0.9913 0.9918 0.993 0.9788 0.9885
Peroxisomes 0.0233 0 0.0012 0.0014 0 0.005 0.0165 0.0001 0.0126 0 0.0005 0.0039 0.0005 0 0 0 0 0
Punctate Nuclear 0.0103 0.0017 0.0021 0.0004 0.072 0.0051 0.0389 0.001 0.0022 0.0014 0.0014 0.0066 0.0057 0.0004 0.0003 0.0001 0.0006 0.0003
Vacuole 0.0035 0.0004 0.0006 0.0007 0.0005 0.0002 0.0211 0.0022 0.0009 0.0001 0.0003 0.0002 0.0011 0.0002 0.0002 0 0.001 0.0003
Vacuole Periphery 0.0014 0.0001 0.0002 0.0008 0.0005 0.0002 0.0084 0.0021 0.0004 0.0001 0.0004 0.0001 0.0008 0.0001 0 0 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.0164 10.0651 26.5554 18.2078 22.7081 22.506 13.569 13.7373 14.5407 19.6207
Translational Efficiency 3.6297 3.7868 1.6857 2.6792 2.3895 2.8302 2.3528 2.8618 2.6925 2.6017

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1917 667 2145 1709 1669 2217 2529 1504 3586 2884 4674 3213

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 858.90 967.46 999.24 1006.99 803.69 908.68 984.54 1027.89 833.20 922.27 991.29 1016.77
Standard Deviation 148.17 160.98 127.51 171.73 126.48 128.35 141.73 158.70 141.21 138.82 135.59 166.09
Intensity Change Log 2 — 0.171712 0.218341 0.229487 — 0.177133 0.292811 0.354975 — 0.174335 0.254820 0.291511

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000180 0.000690 0.000401 0.000722 0.000201 0.000193 0.000291 0.000654 0.000189 0.000308 0.000342 0.000691
Bud Neck 0.003805 0.014377 0.013687 0.025809 0.003797 0.007056 0.013692 0.024787 0.003801 0.008749 0.013690 0.025331
Bud Site 0.000755 0.003302 0.001409 0.006010 0.000639 0.001157 0.000738 0.004969 0.000701 0.001653 0.001046 0.005523
Cell Periphery 0.000254 0.000606 0.000112 0.000237 0.000180 0.000226 0.000111 0.000192 0.000220 0.000314 0.000111 0.000216
Cytoplasm 0.005248 0.006381 0.005978 0.013910 0.007368 0.003449 0.002252 0.005934 0.006234 0.004127 0.003962 0.010176
Cytoplasmic Foci 0.000368 0.001756 0.000033 0.002319 0.000231 0.000630 0.000008 0.000454 0.000304 0.000890 0.000020 0.001446
Eisosomes 0.000015 0.000028 0.000021 0.000030 0.000015 0.000016 0.000023 0.000023 0.000015 0.000019 0.000022 0.000027
Endoplasmic Reticulum 0.006253 0.010774 0.004270 0.004772 0.004933 0.006192 0.003748 0.005345 0.005639 0.007252 0.003988 0.005041
Endosome 0.000315 0.001672 0.000226 0.002594 0.000210 0.000929 0.000105 0.001908 0.000266 0.001101 0.000160 0.002273
Golgi 0.000303 0.000900 0.000034 0.001499 0.000050 0.000453 0.000021 0.000988 0.000185 0.000556 0.000027 0.001260
Lipid Particles 0.000185 0.000250 0.000061 0.000262 0.000123 0.000569 0.000017 0.000087 0.000156 0.000495 0.000037 0.000180
Mitochondria 0.000594 0.003847 0.001355 0.003810 0.001108 0.002057 0.000781 0.002894 0.000833 0.002471 0.001044 0.003381
Mitotic Spindle 0.000554 0.002428 0.001610 0.013772 0.000985 0.003163 0.000660 0.007205 0.000754 0.002993 0.001096 0.010698
None 0.005019 0.004119 0.002247 0.006282 0.004645 0.001959 0.001015 0.003184 0.004845 0.002458 0.001581 0.004832
Nuclear Periphery 0.000537 0.000970 0.000727 0.002633 0.000343 0.001077 0.000511 0.002028 0.000447 0.001052 0.000611 0.002350
Nuclear Periphery Foci 0.000235 0.000442 0.000068 0.001253 0.000283 0.000623 0.000027 0.001021 0.000258 0.000581 0.000046 0.001145
Nucleolus 0.002766 0.004846 0.000856 0.005180 0.002400 0.002617 0.000851 0.003264 0.002596 0.003132 0.000853 0.004283
Nucleus 0.970996 0.937800 0.964605 0.897912 0.971069 0.964644 0.974114 0.928088 0.971030 0.958435 0.969750 0.912038
Peroxisomes 0.000212 0.000563 0.000045 0.000504 0.000095 0.000198 0.000020 0.000102 0.000157 0.000282 0.000032 0.000316
Vacuole 0.001258 0.003507 0.002131 0.007335 0.000788 0.001789 0.000919 0.005307 0.001039 0.002187 0.001475 0.006386
Vacuole Periphery 0.000149 0.000742 0.000125 0.003152 0.000536 0.001007 0.000094 0.001566 0.000329 0.000946 0.000108 0.002410

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.58 -10.23 -13.22 -1.29 -5.82 0.43 -5.25 -13.27 -13.50 -11.62 -4.73 -11.70 -18.55 -12.45 -11.69
Bud Neck -6.78 -11.02 -14.53 -5.29 -6.46 -3.01 -11.47 -14.86 -12.32 -8.20 -5.94 -16.03 -20.78 -14.41 -10.37
Bud Site -2.77 -3.52 -5.31 -1.27 -4.07 -2.16 -0.96 -4.45 -3.82 -4.30 -3.35 -3.33 -6.91 -4.86 -6.05
Cell Periphery -1.27 2.96 1.57 1.62 -2.21 -1.02 3.39 0.87 1.56 -1.37 -1.24 4.00 1.80 2.17 -2.52
Cytoplasm -0.83 -0.55 -4.61 -2.56 -4.65 2.99 4.26 0.92 -2.77 -4.89 2.21 3.22 -2.91 -5.24 -6.75
Cytoplasmic Foci -1.67 2.09 -2.02 0.48 -3.07 -1.80 3.37 -0.61 1.35 -2.90 -2.15 3.13 -2.10 0.13 -3.66
Eisosomes -5.61 -6.41 -6.32 -0.78 -3.27 -0.96 -5.28 -8.15 -7.87 -1.37 -3.69 -7.93 -9.13 -6.07 -3.45
Endoplasmic Reticulum -5.80 3.93 2.85 7.36 -1.35 -3.02 4.74 -1.67 1.54 -5.98 -4.37 6.31 1.10 5.25 -5.59
Endosome -1.68 0.90 -3.56 0.09 -3.71 -3.67 3.77 -2.74 -0.84 -3.01 -3.51 2.92 -4.45 -0.98 -4.80
Golgi -0.96 1.04 -1.54 0.05 -3.35 -1.60 1.45 -2.19 -0.54 -2.32 -1.36 1.16 -2.49 -0.71 -4.02
Lipid Particles -0.56 1.18 -0.45 0.18 -2.52 -1.95 2.02 0.54 2.17 -6.84 -1.92 2.17 -0.14 1.93 -3.97
Mitochondria -2.25 -4.21 -4.74 0.37 -3.20 -1.45 0.50 -2.77 -1.23 -4.81 -3.02 -1.33 -5.33 -0.97 -5.47
Mitotic Spindle -1.51 -1.75 -5.45 -3.68 -4.55 -1.95 0.78 -3.98 -2.49 -4.37 -2.73 -0.74 -6.74 -4.31 -6.36
None 0.86 3.97 -0.02 -0.91 -4.50 3.75 5.45 2.06 -2.51 -5.71 4.19 6.68 1.17 -3.52 -6.80
Nuclear Periphery -2.50 -5.95 -4.23 -2.94 -3.33 -3.22 -4.41 -3.46 -1.62 -2.91 -3.38 -6.74 -5.45 -2.95 -4.49
Nuclear Periphery Foci -0.96 1.75 -2.41 -0.81 -3.08 -1.40 2.34 -1.27 -0.20 -2.35 -1.86 3.04 -2.53 -0.63 -3.85
Nucleolus -2.06 5.34 -3.93 -0.98 -6.41 -0.50 4.28 -4.28 -3.78 -6.30 -1.34 6.68 -5.72 -4.25 -8.91
Nucleus 5.57 3.85 12.90 3.96 10.93 1.62 -0.53 10.79 9.20 12.23 4.30 2.22 16.82 11.44 16.48
Peroxisomes -1.32 1.54 -0.57 0.81 -1.63 -1.61 2.97 -0.48 1.43 -5.15 -1.36 2.24 -0.64 0.51 -2.19
Vacuole -2.99 -3.71 -7.18 -3.86 -5.28 -3.70 -1.49 -6.18 -4.97 -5.99 -3.75 -3.59 -9.47 -7.47 -8.20
Vacuole Periphery -2.41 0.89 -3.05 -1.80 -3.11 -1.06 1.28 -1.10 -0.11 -2.89 -2.21 1.39 -3.09 -1.27 -4.11
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description tRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Trm10

Trm10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available