Standard name | |
---|---|
Human Ortholog | |
Description | O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 14 | 6 | 2 | 1 | 1 | 6 | 27 | 9 | 13 | 23 | 33 |
Bud | 7 | 19 | 13 | 8 | 13 | 15 | 23 | 27 | 24 | 24 | 23 | 30 | 4 | 7 | 5 | 7 | 6 | 5 | 1 | 14 | 3 | 3 | 11 | 18 |
Bud Neck | 0 | 10 | 10 | 0 | 5 | 2 | 5 | 3 | 2 | 4 | 0 | 3 | 10 | 18 | 23 | 2 | 3 | 1 | 0 | 11 | 3 | 7 | 15 | 19 |
Bud Site | 2 | 1 | 1 | 0 | 0 | 8 | 3 | 5 | 3 | 9 | 5 | 9 | 2 | 0 | 0 | 4 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 4 | 3 | 2 | 3 | 9 | 0 | 4 | 2 | 2 | 4 | 6 |
Cytoplasm | 18 | 379 | 179 | 94 | 77 | 69 | 61 | 56 | 40 | 48 | 31 | 44 | 306 | 370 | 459 | 10 | 18 | 22 | 8 | 192 | 66 | 57 | 65 | 63 |
Endoplasmic Reticulum | 0 | 3 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 5 | 2 | 8 | 3 | 2 | 3 | 10 | 0 | 6 | 3 | 3 | 4 | 3 |
Endosome | 11 | 26 | 7 | 6 | 1 | 2 | 0 | 2 | 3 | 5 | 1 | 2 | 15 | 8 | 24 | 21 | 34 | 43 | 20 | 55 | 36 | 58 | 73 | 72 |
Golgi | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 3 | 2 | 5 | 4 | 0 | 5 | 6 | 11 |
Mitochondria | 34 | 58 | 49 | 40 | 100 | 179 | 295 | 233 | 306 | 335 | 321 | 388 | 7 | 1 | 2 | 32 | 16 | 19 | 36 | 180 | 125 | 181 | 249 | 214 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 8 | 4 | 5 | 8 | 2 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 9 | 4 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 |
Nucleolus | 3 | 1 | 0 | 1 | 1 | 2 | 4 | 3 | 5 | 3 | 2 | 9 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 1 | 4 | 0 |
Peroxisomes | 13 | 9 | 8 | 3 | 5 | 3 | 7 | 11 | 9 | 19 | 14 | 9 | 6 | 0 | 3 | 49 | 58 | 63 | 18 | 13 | 8 | 18 | 26 | 30 |
SpindlePole | 25 | 64 | 48 | 24 | 34 | 36 | 51 | 50 | 37 | 57 | 44 | 32 | 31 | 56 | 61 | 71 | 60 | 66 | 15 | 96 | 43 | 49 | 54 | 70 |
Vac/Vac Membrane | 3 | 11 | 3 | 1 | 0 | 2 | 3 | 1 | 6 | 6 | 5 | 2 | 7 | 4 | 8 | 5 | 5 | 9 | 2 | 21 | 5 | 19 | 22 | 29 |
Unique Cell Count | 87 | 537 | 284 | 157 | 200 | 261 | 356 | 314 | 352 | 395 | 374 | 440 | 372 | 461 | 568 | 148 | 152 | 171 | 123 | 652 | 324 | 439 | 576 | 592 |
Labelled Cell Count | 119 | 594 | 321 | 182 | 237 | 325 | 458 | 401 | 441 | 517 | 464 | 542 | 402 | 492 | 599 | 217 | 213 | 253 | 123 | 652 | 324 | 439 | 576 | 592 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 4.0 | 3.4 | 3.6 | 3.3 | 3.3 | 2.7 | 3.1 | 2.9 | 3.1 | 3.0 | 2.9 | 5.1 | 4.7 | 5.0 | 5.8 | 5.7 | 5.6 | 5.4 | 5.5 | 6.2 |
Std Deviation (1e-4) | 3.7 | 0.9 | 1.0 | 1.7 | 1.6 | 1.1 | 0.9 | 1.2 | 0.8 | 1.0 | 0.9 | 0.8 | 1.0 | 0.7 | 1.0 | 1.3 | 1.4 | 1.3 | 1.3 | 1.3 | 11.3 |
Intensity Change (Log2) | – | – | – | 0.08 | -0.04 | -0.03 | -0.33 | -0.11 | -0.22 | -0.14 | -0.18 | -0.2 | 0.59 | 0.49 | 0.58 | 0.78 | 0.75 | 0.74 | 0.68 | 0.69 | 0.87 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3937 | 0.667 | 0.602 | 1.0361 | 0.2778 | 0.7178 | 3.7808 | 3.1038 | 3.4278 | 2.3774 | 3.4083 | 3.4659 | 1.5332 | 1.5124 | 1.5651 | 1.4543 | 1.3869 | 1.384 |
Actin | 0.0107 | 0.0126 | 0.0051 | 0.0199 | 0.0027 | 0.0069 | 0.0965 | 0.0405 | 0.0335 | 0.0465 | 0.0419 | 0.0362 | 0.0124 | 0.0042 | 0.0101 | 0.0039 | 0.0064 | 0.004 |
Bud | 0.0004 | 0.0016 | 0.0008 | 0.0021 | 0.0002 | 0.0006 | 0.001 | 0.0049 | 0.0006 | 0.0005 | 0.0009 | 0.0007 | 0.0004 | 0.001 | 0.0012 | 0.001 | 0.0009 | 0.0003 |
Bud Neck | 0.001 | 0.0009 | 0.0004 | 0.002 | 0.0003 | 0.0011 | 0.0016 | 0.0006 | 0.0122 | 0.0002 | 0.0014 | 0.0024 | 0.001 | 0.0009 | 0.0008 | 0.0003 | 0.0006 | 0.0013 |
Bud Periphery | 0.0008 | 0.0024 | 0.0023 | 0.0052 | 0.0005 | 0.0008 | 0.0015 | 0.0114 | 0.001 | 0.0006 | 0.0012 | 0.0015 | 0.0007 | 0.002 | 0.0028 | 0.004 | 0.0024 | 0.0006 |
Bud Site | 0.0017 | 0.0172 | 0.003 | 0.0013 | 0.0005 | 0.0007 | 0.0053 | 0.0112 | 0.0072 | 0.0007 | 0.0016 | 0.0007 | 0.0054 | 0.0084 | 0.0022 | 0.0014 | 0.0022 | 0.0006 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0005 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0008 | 0.0001 |
Cytoplasm | 0.006 | 0.0043 | 0.0047 | 0.0053 | 0.0053 | 0.0085 | 0.0111 | 0.0048 | 0.0107 | 0.0014 | 0.0015 | 0.0024 | 0.0027 | 0.0057 | 0.0013 | 0.0049 | 0.0091 | 0.0015 |
Cytoplasmic Foci | 0.1637 | 0.1573 | 0.151 | 0.1233 | 0.103 | 0.1336 | 0.1188 | 0.1396 | 0.1549 | 0.0809 | 0.1587 | 0.1305 | 0.1347 | 0.1669 | 0.1294 | 0.0988 | 0.1152 | 0.1233 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.001 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0025 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0011 | 0.0098 | 0.001 | 0.0005 | 0.0003 | 0.0001 | 0.001 | 0.0013 | 0.0004 | 0.0002 | 0.0002 | 0.0023 | 0.0002 |
Endosome | 0.084 | 0.0705 | 0.0524 | 0.0856 | 0.0771 | 0.053 | 0.0746 | 0.0818 | 0.0928 | 0.039 | 0.0485 | 0.0527 | 0.093 | 0.0882 | 0.0744 | 0.0836 | 0.0836 | 0.0612 |
Golgi | 0.0453 | 0.0586 | 0.0323 | 0.0497 | 0.0384 | 0.0366 | 0.0598 | 0.088 | 0.1013 | 0.0367 | 0.0492 | 0.0721 | 0.0725 | 0.0859 | 0.0797 | 0.0755 | 0.0596 | 0.0687 |
Lipid Particles | 0.1445 | 0.0614 | 0.0839 | 0.028 | 0.0457 | 0.1034 | 0.0853 | 0.0386 | 0.0395 | 0.1227 | 0.0173 | 0.0717 | 0.142 | 0.0604 | 0.0671 | 0.0414 | 0.079 | 0.092 |
Mitochondria | 0.1059 | 0.1193 | 0.1782 | 0.2697 | 0.1674 | 0.1437 | 0.0658 | 0.0868 | 0.1296 | 0.1497 | 0.0838 | 0.0829 | 0.1349 | 0.1615 | 0.2215 | 0.1107 | 0.1358 | 0.1649 |
None | 0.0469 | 0.0249 | 0.0454 | 0.0335 | 0.0628 | 0.0491 | 0.0125 | 0.0099 | 0.0017 | 0.0007 | 0.0013 | 0.0283 | 0.0088 | 0.0126 | 0.0091 | 0.003 | 0.0273 | 0.009 |
Nuclear Periphery | 0.0071 | 0.0005 | 0.0003 | 0.0004 | 0.001 | 0.0015 | 0.0035 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0012 | 0.0027 | 0.0002 | 0.0003 | 0.0001 | 0.0053 | 0.0003 |
Nucleolus | 0.0011 | 0.0008 | 0.0009 | 0.0002 | 0.0012 | 0.0011 | 0.0002 | 0.0003 | 0.0024 | 0.0003 | 0.0001 | 0.0002 | 0.001 | 0.0035 | 0.0004 | 0.0002 | 0.0005 | 0.0003 |
Nucleus | 0.0014 | 0.0005 | 0.0004 | 0.0002 | 0.0015 | 0.0011 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0022 | 0.0004 | 0.0002 | 0.0001 | 0.0009 | 0.0002 |
Peroxisomes | 0.3658 | 0.4594 | 0.4203 | 0.3666 | 0.4854 | 0.4425 | 0.4432 | 0.4755 | 0.4009 | 0.5162 | 0.5895 | 0.51 | 0.3774 | 0.3907 | 0.3893 | 0.5662 | 0.4581 | 0.4645 |
Punctate Nuclear | 0.0047 | 0.0026 | 0.0126 | 0.0007 | 0.0029 | 0.01 | 0.0041 | 0.0009 | 0.002 | 0.0011 | 0.0011 | 0.0024 | 0.0035 | 0.0023 | 0.0065 | 0.0021 | 0.0062 | 0.004 |
Vacuole | 0.0035 | 0.0034 | 0.0034 | 0.0028 | 0.0021 | 0.0027 | 0.0018 | 0.0013 | 0.005 | 0.0009 | 0.0008 | 0.0011 | 0.0017 | 0.0031 | 0.0016 | 0.0012 | 0.002 | 0.0016 |
Vacuole Periphery | 0.0024 | 0.0011 | 0.0016 | 0.0024 | 0.0011 | 0.0013 | 0.0011 | 0.0009 | 0.0022 | 0.0008 | 0.0006 | 0.0009 | 0.0012 | 0.0013 | 0.0013 | 0.001 | 0.0013 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.2256 | 6.3027 | 8.5743 | 11.2433 | 11.4266 | 12.9188 | 11.6832 | 17.2045 | 17.0257 | 13.7441 |
Translational Efficiency | 2.0133 | 1.9545 | 1.4458 | 1.6341 | 1.1437 | 1.3055 | 1.3868 | 1.0794 | 1.3169 | 1.3262 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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