Standard name
Human Ortholog
Description tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.97 1.0 0.95 0.93 0.91 – 0.77 0.75 0.64 0.67 1.0 0.99 1.0 – 0.98 0.95 0.84 0.84 0.84 0.88 0.86 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.07 – 0.42 0.38 0.52 0.46 0 0 0 – 0 0.06 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 2 1 0 0 0 3
Bud 1 2 0 1 0 2 1 – 1 4 6 2 0 0 0 – 0 0 4 2 0 4 3 10
Bud Neck 0 0 1 0 4 1 1 – 2 3 1 1 0 0 0 – 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 1 0 – 0 1 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 2 – 0 0 1 1 0 1 0 – 3 3 1 0 1 1 0 1
Cytoplasm 205 212 33 16 101 93 160 – 115 122 104 172 175 281 314 – 88 92 188 190 54 160 180 230
Endoplasmic Reticulum 1 1 0 0 1 0 1 – 1 0 0 2 0 1 0 – 7 6 0 1 0 3 2 2
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 3 1 0 0 1 2
Golgi 0 0 1 0 1 0 0 – 0 0 0 0 0 1 0 – 0 0 1 1 1 0 2 2
Mitochondria 7 4 0 0 1 15 12 – 63 61 85 117 0 1 1 – 2 6 12 5 2 4 9 4
Nucleus 1 1 0 0 0 1 0 – 3 1 4 3 0 0 0 – 0 3 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 1 – 0 1 4 4 0 0 0 – 0 0 2 1 0 1 1 4
Vac/Vac Membrane 0 0 0 0 2 1 4 – 4 4 3 3 0 0 0 – 1 1 3 14 0 1 2 4
Unique Cell Count 211 216 34 16 106 100 175 149 162 163 257 175 283 314 90 97 225 226 65 182 209 276
Labelled Cell Count 215 221 35 17 112 114 182 190 197 209 307 175 285 315 101 111 225 226 65 182 209 276


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.2 4.8 3.7 5.5 3.8 4.6 – 3.9 4.1 3.8 3.8 4.7 4.9 4.9 4.9 5.2 5.1 4.7 5.3 4.8
Std Deviation (1e-4) 1.3 0.9 0.7 0.2 2.2 1.7 1.8 – 1.8 1.7 1.6 1.4 1.6 1.2 1.1 0.8 1.1 1.2 1.6 9.5 1.9
Intensity Change (Log2) – – – -0.38 0.18 -0.34 -0.09 – -0.3 -0.24 -0.35 -0.35 -0.03 0.01 0.02 0.01 0.1 0.09 -0.05 0.14 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0722 -0.3496 -0.8149 -1.0129 -1.0194 -0.9428 0.8066 1.039 0.8556 0.6612 0.9583 0.8346 1.9962 2.8996 2.6389 2.709 2.3932 2.6403
Actin 0.0232 0.001 0.0073 0.0422 0.006 0.0109 0.0262 0.0022 0.0071 0.0054 0.0002 0.008 0.0136 0.0004 0.0001 0.0061 0.0002 0.0003
Bud 0.0005 0.0018 0.0001 0.0002 0.0002 0.0019 0.0026 0.0044 0.0041 0.0046 0.0001 0.0016 0.0003 0.0001 0 0.0003 0 0
Bud Neck 0.0016 0.0001 0.0076 0.0004 0.0002 0.0008 0.0112 0.0002 0.0007 0.0002 0.0001 0.002 0.0047 0 0 0.0012 0.0001 0.0002
Bud Periphery 0.001 0.0017 0.0002 0.0003 0.0003 0.0031 0.0053 0.0045 0.0055 0.0265 0 0.0025 0.0006 0.0001 0 0.0004 0 0
Bud Site 0.0022 0.0023 0.0009 0.0008 0.0009 0.001 0.0138 0.009 0.0093 0.0006 0.0001 0.0005 0.0016 0.0002 0.0001 0.0011 0 0
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0009 0.0002 0.0005 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0 0
Cytoplasm 0.1239 0.2056 0.1698 0.3057 0.1451 0.2226 0.2335 0.3723 0.4128 0.3786 0.3706 0.4485 0.1978 0.391 0.2932 0.2448 0.4685 0.4043
Cytoplasmic Foci 0.0223 0.0092 0.009 0.009 0.0142 0.0178 0.0248 0.0105 0.0168 0.0086 0.0314 0.0204 0.0066 0.0045 0.0025 0.0046 0.0057 0.0051
Eisosomes 0.0006 0.0003 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0 0 0.0003 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0024 0.0009 0.0017 0.002 0.0016 0.0015 0.0091 0.0011 0.0016 0.0011 0.0007 0.0014 0.0011 0.0012 0.0008 0.0028 0.0016 0.0008
Endosome 0.0176 0.0021 0.0067 0.0061 0.0029 0.0067 0.0442 0.0079 0.0109 0.012 0.0295 0.0063 0.0025 0.0012 0.0004 0.0134 0.0015 0.0018
Golgi 0.0045 0.0005 0.0016 0.0049 0.0049 0.0016 0.0107 0.0032 0.0039 0.0057 0.0042 0.0027 0.0011 0.0001 0 0.0035 0.0001 0.0002
Lipid Particles 0.007 0.0003 0.0022 0.0058 0.0039 0.0025 0.0128 0.0009 0.0064 0.0006 0.0002 0.0029 0.0034 0.0002 0.0001 0.0017 0.0001 0.0001
Mitochondria 0.0018 0.0196 0.0016 0.0024 0.0319 0.0028 0.0042 0.0023 0.0022 0.0375 0.0017 0.0048 0.0006 0.0001 0.0001 0.0009 0.0001 0.0007
None 0.7639 0.7503 0.7822 0.6038 0.756 0.7157 0.5461 0.568 0.5083 0.5111 0.4848 0.486 0.7556 0.5992 0.7013 0.7116 0.5205 0.5848
Nuclear Periphery 0.0039 0.0003 0.0018 0.0017 0.0009 0.0018 0.0098 0.0009 0.0014 0.0009 0.0277 0.0018 0.0014 0.0002 0.0002 0.0023 0.0004 0.0002
Nucleolus 0.001 0.0003 0.0003 0.0001 0.0003 0.0003 0.0054 0.0005 0.0003 0.0001 0.0003 0.0002 0.0003 0 0 0.0003 0 0
Nucleus 0.003 0.001 0.0013 0.0011 0.0007 0.0012 0.0072 0.0019 0.0014 0.0012 0.0399 0.0024 0.0005 0.0006 0.0004 0.0014 0.0006 0.0006
Peroxisomes 0.0107 0.0005 0.0021 0.0102 0.0278 0.0043 0.0109 0.0047 0.0022 0.0009 0.0057 0.0037 0.0041 0.0001 0 0.0004 0 0.0001
Punctate Nuclear 0.0064 0.0007 0.002 0.001 0.0008 0.0022 0.0063 0.0042 0.0012 0.0006 0.0007 0.0031 0.0031 0.0002 0.0002 0.0014 0.0003 0.0003
Vacuole 0.0019 0.0011 0.0009 0.0014 0.0004 0.0006 0.0118 0.0008 0.0028 0.002 0.0008 0.0008 0.0004 0.0004 0.0003 0.0012 0.0003 0.0002
Vacuole Periphery 0.0005 0.0002 0.0003 0.0005 0.001 0.0003 0.0029 0.0002 0.0007 0.0016 0.0012 0.0002 0.0002 0 0 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0871 7.1167 11.618 13.0887 11.3541 14.322 12.5925 12.1415 10.901 20.4007
Translational Efficiency 1.5633 3.4138 1.416 1.2047 2.5623 2.6051 1.602 1.5521 1.8715 1.6418

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1514 348 1853 121 1924 2855 405 1269 3438 3203 2258 1390

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 545.40 801.30 866.45 890.37 675.51 750.80 891.22 830.48 618.21 756.29 870.89 835.69
Standard Deviation 67.52 96.61 102.85 119.68 72.15 120.17 92.95 125.70 95.36 118.89 101.59 126.32
Intensity Change Log 2 — 0.555028 0.667802 0.707090 — 0.152452 0.399804 0.297968 — 0.346265 0.525707 0.495164

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000296 0.001179 0.000201 0.000470 0.000060 0.000304 0.000280 0.001750 0.000164 0.000399 0.000215 0.001639
Bud Neck 0.038469 0.042185 0.005556 0.005310 0.009488 0.041191 0.001433 0.009519 0.022251 0.041299 0.004816 0.009152
Bud Site 0.015138 0.036471 0.012117 0.009701 0.002467 0.019315 0.001680 0.040843 0.008047 0.021179 0.010245 0.038132
Cell Periphery 0.000786 0.000261 0.000107 0.000206 0.000125 0.000156 0.000144 0.000219 0.000416 0.000168 0.000114 0.000218
Cytoplasm 0.340054* 0.311356* 0.463037* 0.399953* 0.545016* 0.534945* 0.314320* 0.515528* 0.454756* 0.510652* 0.436362* 0.505467*
Cytoplasmic Foci 0.295515* 0.286584* 0.007725 0.021384 0.177284 0.152589 0.010180 0.027523 0.229350* 0.167147 0.008165 0.026989
Eisosomes 0.000748 0.000187 0.000023 0.000075 0.000125 0.000102 0.000059 0.000053 0.000399 0.000111 0.000029 0.000055
Endoplasmic Reticulum 0.002855 0.001564 0.001853 0.002740 0.000626 0.000391 0.004914 0.001699 0.001608 0.000518 0.002402 0.001789
Endosome 0.008703 0.035133 0.001716 0.016675 0.006206 0.010846 0.002215 0.007763 0.007306 0.013485 0.001805 0.008539
Golgi 0.011670 0.007387 0.000092 0.001340 0.001098 0.002727 0.000059 0.008780 0.005754 0.003233 0.000086 0.008133
Lipid Particles 0.028459 0.003748 0.000763 0.002880 0.006742 0.002732 0.001935 0.001934 0.016305 0.002843 0.000974 0.002017
Mitochondria 0.087847 0.012445 0.000656 0.012859 0.001614 0.007994 0.000354 0.002609 0.039589 0.008477 0.000602 0.003501
Mitotic Spindle 0.000176 0.006188 0.006314 0.015365 0.000667 0.007568 0.000451 0.041413 0.000451 0.007418 0.005262 0.039145
None 0.011150 0.001014 0.004281 0.005907 0.002648 0.003303 0.006683 0.005064 0.006392 0.003054 0.004712 0.005137
Nuclear Periphery 0.000409 0.000359 0.001438 0.001812 0.000409 0.000257 0.001693 0.001763 0.000409 0.000268 0.001484 0.001767
Nuclear Periphery Foci 0.000254 0.000560 0.000753 0.002643 0.000182 0.000113 0.002130 0.001568 0.000214 0.000162 0.001000 0.001661
Nucleolus 0.001404 0.001259 0.000391 0.000508 0.000596 0.001345 0.000465 0.000863 0.000952 0.001336 0.000404 0.000832
Nucleus 0.116280 0.092310 0.448958* 0.299638* 0.212361* 0.147931* 0.568760* 0.217659* 0.170049* 0.141888* 0.470446* 0.224795*
Peroxisomes 0.018668 0.035728 0.000185 0.000718 0.003099 0.008096 0.000210 0.001430 0.009955 0.011098 0.000189 0.001368
Vacuole 0.017692 0.121583 0.042976 0.194579 0.028777 0.056801 0.081909 0.107115 0.023895 0.063839 0.049959 0.114729
Vacuole Periphery 0.003426 0.002497 0.000859 0.005237 0.000409 0.001294 0.000126 0.004907 0.001738 0.001425 0.000727 0.004936

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.98 0.97 -0.72 4.41 -1.26 -3.98 -8.79 -2.15 -1.53 -1.21 -3.96 -4.12 -1.94 -1.30 -1.60
Bud Neck -0.49 13.77 14.07 7.88 1.41 -15.87 8.79 1.15 16.42 -6.87 -9.30 13.33 10.61 17.84 -2.84
Bud Site -4.61 2.09 2.51 5.81 0.98 -12.18 -0.98 -7.89 -1.67 -7.68 -8.94 -0.75 -5.66 -0.65 -4.92
Cell Periphery 6.81 10.02 9.25 2.54 -2.17 -1.17 -2.43 -2.25 -0.02 -0.36 6.39 9.76 7.91 0.40 -3.03
Cytoplasm 1.93 -8.40 2.35 1.01 5.39 1.43 21.73 12.48 11.57 -11.38 -8.07 9.18 4.98 10.74 -2.34
Cytoplasmic Foci 0.79 51.18 43.60 21.61 -4.04 4.52 39.64 36.16 35.25 -7.31 12.56 62.34 55.98 39.91 -10.15
Eisosomes 13.63 19.10 16.86 4.34 -9.99 3.57 6.10 11.35 8.22 3.39 15.99 20.37 18.84 9.42 -10.08
Endoplasmic Reticulum 2.94 0.53 -2.17 -5.05 -3.18 1.69 -12.89 -9.58 -12.22 8.88 5.84 -8.53 -4.70 -12.90 3.79
Endosome -6.58 12.89 0.72 6.04 -2.06 -5.42 5.60 1.83 7.37 -3.55 -7.51 12.71 3.54 9.52 -5.30
Golgi 2.71 16.76 15.55 4.22 -1.38 -5.25 6.76 -2.27 -0.93 -3.14 5.83 17.27 1.90 -0.33 -3.16
Lipid Particles 16.46 18.75 17.47 3.14 -5.29 7.56 8.70 8.77 2.84 1.26 18.03 20.78 18.83 3.28 -2.51
Mitochondria 12.86 18.52 15.29 1.89 -0.85 -8.30 6.75 -0.06 8.10 -5.15 13.39 17.71 17.00 7.90 -3.32
Mitotic Spindle -3.41 -5.05 -1.64 -0.03 -0.02 -6.24 -1.15 -8.25 -5.82 -7.95 -7.04 -4.89 -8.44 -5.80 -6.55
None 7.20 5.13 5.33 -3.21 1.39 -1.45 -2.80 -3.15 -1.90 0.32 4.88 3.62 2.90 -2.35 -0.43
Nuclear Periphery 0.61 -22.34 -7.85 -8.01 -1.72 3.30 -15.43 -15.22 -17.00 -1.29 4.07 -28.16 -16.58 -18.07 -4.40
Nuclear Periphery Foci -3.08 -8.71 -7.95 -6.93 -5.86 4.47 -7.25 -6.61 -6.97 3.58 1.57 -12.42 -7.75 -8.08 -1.06
Nucleolus 0.63 4.65 5.29 4.86 2.18 -3.09 2.96 -0.42 2.66 -1.95 -1.66 5.29 2.41 3.03 -1.83
Nucleus 2.73 -45.96 -5.33 -6.11 9.63 12.10 -22.54 -6.88 -16.03 17.82 6.57 -46.57 -13.29 -17.30 25.00
Peroxisomes -3.64 16.24 16.04 8.08 -1.55 -6.85 6.39 4.56 11.68 -2.09 -1.19 16.93 14.89 13.85 -2.21
Vacuole -12.46 -16.65 -13.43 -9.24 -11.57 -13.61 -12.10 -25.03 -20.43 -3.53 -20.07 -18.95 -28.21 -21.57 -17.62
Vacuole Periphery 2.24 6.55 1.81 0.92 -0.58 -9.05 8.97 -2.81 -1.47 -3.23 2.18 3.97 -0.72 -1.25 -2.44
Standard name
Human Ortholog
Description tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
Localization
Cell Percentages cytoplasm (20%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tpt1

Tpt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available