ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 2 | 2 | 0 | 0 | 15 | 0 | 4 | – | 2 | 3 | 2 | 9 | 9 | 4 | 3 | 2 | 7 | 4 | 4 | 1 | 1 | 6 | 9 |
Bud | 1 | 1 | 7 | 12 | 18 | 40 | 34 | 33 | – | 44 | 50 | 48 | 4 | 4 | 7 | 6 | 6 | 3 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 1 | 0 | 0 | 1 | 8 | 8 | 4 | – | 6 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 1 | 0 | 0 | 3 | 5 | 2 | – | 2 | 3 | 5 | 0 | 3 | 2 | 0 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 6 | 10 | 17 | 19 | 42 | 32 | 31 | – | 24 | 11 | 8 | 12 | 30 | 33 | 9 | 11 | 14 | 0 | 1 | 3 | 0 | 2 | 3 |
Endoplasmic Reticulum | 5 | 1 | 8 | 6 | 12 | 24 | 12 | 2 | – | 5 | 1 | 9 | 7 | 21 | 12 | 2 | 6 | 7 | 1 | 0 | 0 | 0 | 1 | 2 |
Endosome | 51 | 4 | 9 | 14 | 24 | 5 | 8 | 19 | – | 16 | 0 | 4 | 26 | 71 | 97 | 36 | 41 | 41 | 56 | 18 | 36 | 24 | 49 | 35 |
Golgi | 31 | 22 | 4 | 3 | 4 | 3 | 1 | 3 | – | 1 | 0 | 5 | 8 | 82 | 79 | 75 | 92 | 78 | 144 | 62 | 100 | 50 | 124 | 122 |
Mitochondria | 168 | 83 | 184 | 241 | 375 | 605 | 767 | 866 | – | 670 | 599 | 672 | 79 | 128 | 102 | 15 | 15 | 18 | 40 | 22 | 36 | 36 | 57 | 56 |
Nucleus | 2 | 0 | 0 | 0 | 0 | 7 | 3 | 7 | – | 4 | 4 | 7 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 2 | 4 | 6 | 6 | – | 6 | 4 | 12 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | 4 | 2 | 0 | 1 | 3 | 7 | 12 | 47 | – | 17 | 11 | 39 | 1 | 0 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 7 | 1 | 5 | 6 | 10 | 9 | 24 | 36 | – | 24 | 20 | 21 | 6 | 15 | 9 | 4 | 4 | 9 | 19 | 2 | 23 | 7 | 18 | 19 |
SpindlePole | 2 | 0 | 7 | 10 | 11 | 14 | 24 | 32 | – | 29 | 29 | 20 | 4 | 15 | 25 | 8 | 10 | 14 | 3 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 12 | 5 | 1 | 3 | 6 | 5 | 10 | 18 | – | 14 | 11 | 12 | 14 | 32 | 43 | 10 | 14 | 24 | 5 | 1 | 2 | 3 | 7 | 7 |
Unique Cell Count | 228 | 100 | 202 | 269 | 402 | 663 | 822 | 959 | 739 | 632 | 726 | 130 | 318 | 325 | 141 | 172 | 176 | 281 | 116 | 206 | 134 | 277 | 266 | |
Labelled Cell Count | 296 | 129 | 239 | 313 | 485 | 791 | 946 | 1110 | 864 | 755 | 875 | 170 | 418 | 414 | 169 | 207 | 224 | 281 | 116 | 206 | 134 | 277 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 7.0 | 5.0 | 4.7 | 4.6 | 4.7 | 4.4 | 5.0 | – | 4.8 | 4.4 | 4.7 | 6.6 | 6.6 | 6.8 | 8.9 | 9.0 | 10.1 | 7.5 | 7.8 | 7.6 |
Std Deviation (1e-4) | 1.2 | 1.4 | 1.1 | 1.2 | 0.9 | 1.2 | 1.1 | 1.2 | – | 1.1 | 1.0 | 1.2 | 1.1 | 1.1 | 1.2 | 1.5 | 1.7 | 13.6 | 1.4 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.11 | -0.1 | -0.18 | -0.01 | – | -0.07 | -0.17 | -0.1 | 0.4 | 0.4 | 0.44 | 0.83 | 0.85 | 1.02 | 0.59 | 0.65 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | -0.9 | 0.4 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0.6 | 1.3 | -0.2 | 0 | -0.2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 4.5 | 5.5 | 7.1 | 5.7 | 5.5 | 5.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 7.1 | 6.8 | 11.1 | 11.4 | 10.0 |
Mitochondria | 0 | 0 | 0 | 0 | -0.3 | – | 0 | 0 | – | -6.6 | -11.5 | -13.4 | -14.9 | -15.9 | -15.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 3.2 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0.9 | – | 0 | 0 | – | 0 | 1.3 | 0.2 | 0 | 0 | 1.4 |
SpindlePole | 0 | 0 | 0 | 0 | -0.1 | – | 0 | 0 | – | 0 | 0.7 | 2.0 | 1.0 | 1.1 | 1.9 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -0.1 | 0.7 | -0.7 | -1.2 | – | -1.1 | -2.5 | – | 1.5 | 1.9 | 2.1 | 0.6 | 0.6 | 1.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 4.4 | 4.4 | 5.1 | 0 | 3.8 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0689 | 1.1483 | 1.1768 | 1.297 | 0.0373 | 0.8653 | 1.8923 | 2.2614 | 2.3467 | 2.1347 | 2.5496 | 2.4755 | -0.4139 | 0.1305 | 0.3606 | 0.3339 | -0.3387 | 0.0463 |
Actin | 0.0267 | 0.0062 | 0.0244 | 0.0093 | 0.0123 | 0.0189 | 0.0189 | 0.0191 | 0.0266 | 0.0019 | 0.0185 | 0.0084 | 0.0149 | 0.0221 | 0.0112 | 0.0031 | 0.0027 | 0.0067 |
Bud | 0.0015 | 0.0037 | 0.0007 | 0.0001 | 0.0002 | 0.0028 | 0.0002 | 0.0014 | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0001 |
Bud Neck | 0.0026 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.0009 | 0.0006 | 0.0005 | 0.0029 | 0.0005 | 0.0007 | 0.0007 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0007 |
Bud Periphery | 0.0027 | 0.002 | 0.0012 | 0.0001 | 0.0002 | 0.0022 | 0.0003 | 0.0018 | 0.002 | 0.0005 | 0.0014 | 0.0004 | 0.0002 | 0.0006 | 0.0004 | 0.0013 | 0.0004 | 0.0001 |
Bud Site | 0.0074 | 0.0148 | 0.0043 | 0.0005 | 0.0008 | 0.0004 | 0.0016 | 0.0045 | 0.0027 | 0.0006 | 0.0025 | 0.0005 | 0.0009 | 0.0021 | 0.0008 | 0.0003 | 0.0002 | 0.0002 |
Cell Periphery | 0.0005 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0195 | 0.0308 | 0.0109 | 0.0086 | 0.0117 | 0.0133 | 0.022 | 0.03 | 0.0173 | 0.011 | 0.0111 | 0.0134 | 0.0076 | 0.0241 | 0.0131 | 0.0074 | 0.007 | 0.0092 |
Cytoplasmic Foci | 0.1756 | 0.2046 | 0.1682 | 0.1946 | 0.1762 | 0.1921 | 0.1902 | 0.2036 | 0.1806 | 0.2171 | 0.2223 | 0.1697 | 0.1863 | 0.1997 | 0.168 | 0.1356 | 0.1652 | 0.1867 |
Eisosomes | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0002 | 0.0011 | 0.0001 | 0.0005 | 0.0001 | 0.0051 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0028 | 0.0015 | 0.0014 | 0.0009 | 0.0007 | 0.0008 | 0.0021 | 0.0016 | 0.0017 | 0.001 | 0.0014 | 0.0008 | 0.0011 | 0.0009 | 0.0014 | 0.0026 | 0.0007 | 0.0009 |
Endosome | 0.3119 | 0.3161 | 0.3046 | 0.3848 | 0.3544 | 0.236 | 0.2309 | 0.2804 | 0.2812 | 0.3472 | 0.2645 | 0.1916 | 0.3222 | 0.2954 | 0.2927 | 0.3794 | 0.3666 | 0.2351 |
Golgi | 0.1733 | 0.2246 | 0.2489 | 0.2374 | 0.1779 | 0.2346 | 0.2355 | 0.266 | 0.2802 | 0.2686 | 0.2747 | 0.3188 | 0.2836 | 0.2934 | 0.3714 | 0.3459 | 0.3567 | 0.3767 |
Lipid Particles | 0.0839 | 0.0143 | 0.0281 | 0.045 | 0.1288 | 0.0381 | 0.0535 | 0.0232 | 0.0236 | 0.0287 | 0.0501 | 0.0449 | 0.0506 | 0.0339 | 0.0164 | 0.0125 | 0.017 | 0.0321 |
Mitochondria | 0.0504 | 0.054 | 0.0603 | 0.0218 | 0.0114 | 0.061 | 0.0402 | 0.0581 | 0.0545 | 0.0252 | 0.0181 | 0.0546 | 0.0339 | 0.0375 | 0.0455 | 0.0509 | 0.0092 | 0.0469 |
None | 0.057 | 0.0407 | 0.0565 | 0.0049 | 0.0415 | 0.0732 | 0.0786 | 0.0185 | 0.0448 | 0.0251 | 0.0303 | 0.0609 | 0.0075 | 0.0233 | 0.0253 | 0.0128 | 0.0227 | 0.0249 |
Nuclear Periphery | 0.0049 | 0.0005 | 0.0035 | 0.0019 | 0.0006 | 0.0004 | 0.0014 | 0.0007 | 0.0006 | 0.0004 | 0.0023 | 0.0003 | 0.0008 | 0.0003 | 0.0003 | 0.0012 | 0.0003 | 0.0002 |
Nucleolus | 0.0009 | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0069 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0014 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0011 | 0.0003 | 0.0004 | 0.0003 | 0.0014 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 |
Peroxisomes | 0.0633 | 0.0774 | 0.0755 | 0.0756 | 0.0684 | 0.1191 | 0.1128 | 0.0816 | 0.0713 | 0.0611 | 0.0684 | 0.1292 | 0.0831 | 0.0602 | 0.0471 | 0.038 | 0.0457 | 0.0746 |
Punctate Nuclear | 0.0026 | 0.0009 | 0.0012 | 0.0083 | 0.002 | 0.0011 | 0.0028 | 0.0005 | 0.001 | 0.0005 | 0.0145 | 0.0016 | 0.0009 | 0.0004 | 0.0005 | 0.0005 | 0.0007 | 0.0006 |
Vacuole | 0.0068 | 0.0035 | 0.005 | 0.0031 | 0.0094 | 0.0023 | 0.0036 | 0.0051 | 0.004 | 0.0065 | 0.003 | 0.0016 | 0.003 | 0.0028 | 0.0028 | 0.0037 | 0.0023 | 0.002 |
Vacuole Periphery | 0.0035 | 0.0019 | 0.0026 | 0.0022 | 0.0025 | 0.0018 | 0.0013 | 0.0023 | 0.0021 | 0.0031 | 0.0017 | 0.0012 | 0.0018 | 0.0015 | 0.0019 | 0.0031 | 0.002 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.0123 | 11.5029 | 9.247 | 11.3196 | 13.9029 | 8.5737 | 13.0844 | 11.1207 | 9.0488 | 16.6467 |
Translational Efficiency | 0.9289 | 1.0419 | 1.0478 | 0.8117 | 0.8465 | 1.6362 | 0.9542 | 1.0289 | 1.4692 | 0.934 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2056 | 1454 | 1768 | 230 | 628 | 1809 | 306 | 1605 | 2684 | 3263 | 2074 | 1835 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 803.51 | 826.43 | 1107.85 | 974.61 | 713.45 | 770.14 | 1049.79 | 931.40 | 782.44 | 795.22 | 1099.28 | 936.82 |
Standard Deviation | 105.96 | 127.25 | 164.11 | 120.24 | 112.40 | 102.04 | 122.84 | 136.95 | 114.06 | 117.35 | 160.03 | 135.73 |
Intensity Change Log 2 | — | 0.040577 | 0.463375 | 0.278509 | — | 0.110308 | 0.557217 | 0.384589 | — | 0.073793 | 0.508271 | 0.329373 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002916 | 0.002035 | 0.002589 | 0.011789 | 0.001532 | 0.001518 | 0.010409 | 0.012847 | 0.002593 | 0.001749 | 0.003743 | 0.012714 |
Bud Neck | 0.000310 | 0.002222 | 0.000383 | 0.005992 | 0.004453 | 0.002725 | 0.001745 | 0.006971 | 0.001279 | 0.002501 | 0.000584 | 0.006848 |
Bud Site | 0.001905 | 0.007199 | 0.002679 | 0.029408 | 0.003762 | 0.004793 | 0.009240 | 0.030126 | 0.002340 | 0.005865 | 0.003647 | 0.030036 |
Cell Periphery | 0.000119 | 0.000075 | 0.000036 | 0.000084 | 0.000169 | 0.000077 | 0.000062 | 0.000107 | 0.000130 | 0.000076 | 0.000040 | 0.000104 |
Cytoplasm | 0.002643 | 0.030048 | 0.001439 | 0.054715 | 0.021655 | 0.021940 | 0.009776 | 0.075226 | 0.007092 | 0.025553 | 0.002669 | 0.072655 |
Cytoplasmic Foci | 0.193944* | 0.289702* | 0.299775* | 0.296145* | 0.254766* | 0.260577* | 0.347851* | 0.301943* | 0.208175* | 0.273555* | 0.306869* | 0.301216* |
Eisosomes | 0.000075 | 0.000072 | 0.000023 | 0.000083 | 0.000209 | 0.000154 | 0.000049 | 0.000111 | 0.000106 | 0.000117 | 0.000027 | 0.000107 |
Endoplasmic Reticulum | 0.000350 | 0.000433 | 0.000613 | 0.003383 | 0.000565 | 0.000255 | 0.001073 | 0.000902 | 0.000400 | 0.000334 | 0.000681 | 0.001213 |
Endosome | 0.161920 | 0.151270 | 0.146211 | 0.171762 | 0.149465 | 0.132962 | 0.230963* | 0.177404 | 0.159005 | 0.141120 | 0.158716 | 0.176697 |
Golgi | 0.559349* | 0.404611* | 0.468573* | 0.093968 | 0.408566* | 0.451678* | 0.240377* | 0.181060* | 0.524069* | 0.430705* | 0.434904* | 0.170144* |
Lipid Particles | 0.007383 | 0.011594 | 0.007858 | 0.012579 | 0.043987 | 0.015783 | 0.017976 | 0.006308 | 0.015948 | 0.013916 | 0.009351 | 0.007094 |
Mitochondria | 0.044377 | 0.041061 | 0.003722 | 0.004482 | 0.045609 | 0.054630 | 0.006775 | 0.014245 | 0.044665 | 0.048584 | 0.004172 | 0.013022 |
Mitotic Spindle | 0.001721 | 0.014290 | 0.031977 | 0.084374 | 0.003586 | 0.007973 | 0.053643 | 0.043564 | 0.002157 | 0.010788 | 0.035173 | 0.048679 |
None | 0.000053 | 0.000284 | 0.000020 | 0.000317 | 0.000845 | 0.000389 | 0.000040 | 0.000398 | 0.000238 | 0.000342 | 0.000023 | 0.000387 |
Nuclear Periphery | 0.000016 | 0.000062 | 0.000443 | 0.000520 | 0.000132 | 0.000049 | 0.000343 | 0.000265 | 0.000043 | 0.000055 | 0.000428 | 0.000297 |
Nuclear Periphery Foci | 0.000095 | 0.000317 | 0.003255 | 0.003847 | 0.000731 | 0.000137 | 0.002135 | 0.000815 | 0.000244 | 0.000218 | 0.003090 | 0.001195 |
Nucleolus | 0.000027 | 0.000251 | 0.000036 | 0.000450 | 0.001515 | 0.000174 | 0.000419 | 0.000329 | 0.000375 | 0.000208 | 0.000092 | 0.000344 |
Nucleus | 0.000072 | 0.001283 | 0.000060 | 0.022998 | 0.004871 | 0.001631 | 0.001296 | 0.006628 | 0.001195 | 0.001476 | 0.000242 | 0.008680 |
Peroxisomes | 0.016351 | 0.022065 | 0.002544 | 0.053135 | 0.040756 | 0.030853 | 0.036935 | 0.062806 | 0.022062 | 0.026937 | 0.007618 | 0.061593 |
Vacuole | 0.002813 | 0.016365 | 0.020565 | 0.144618 | 0.007600 | 0.007869 | 0.025282 | 0.071900 | 0.003933 | 0.011655 | 0.021261 | 0.081014 |
Vacuole Periphery | 0.003562 | 0.004760 | 0.007200 | 0.005351 | 0.005222 | 0.003834 | 0.003609 | 0.006046 | 0.003950 | 0.004246 | 0.006670 | 0.005959 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.49 | 0.42 | -2.52 | -2.86 | -2.62 | 0.03 | -2.42 | -6.77 | -6.82 | -0.39 | 1.96 | -1.52 | -6.44 | -7.22 | -5.19 |
Bud Neck | -5.73 | -1.18 | -5.93 | -4.31 | -5.87 | 1.34 | 1.78 | -2.51 | -6.69 | -7.43 | -3.20 | 2.07 | -9.82 | -8.15 | -11.78 |
Bud Site | -5.31 | -1.42 | -6.13 | -4.99 | -5.97 | -0.80 | -3.00 | -11.32 | -11.37 | -6.97 | -4.75 | -2.52 | -14.15 | -12.18 | -13.47 |
Cell Periphery | 1.07 | 2.15 | 0.64 | -1.27 | -6.87 | 4.09 | 4.81 | 2.65 | -1.34 | -2.08 | 1.75 | 3.02 | 0.90 | -1.57 | -4.41 |
Cytoplasm | -10.30 | 1.26 | -7.69 | -4.69 | -7.78 | -0.10 | 1.84 | -12.46 | -13.51 | -12.99 | -10.29 | 3.50 | -19.07 | -13.90 | -20.06 |
Cytoplasmic Foci | -18.02 | -19.71 | -3.81 | 2.88 | 3.49 | -0.59 | -6.31 | -3.77 | -4.24 | 4.10 | -15.78 | -19.20 | -13.51 | -1.73 | 3.30 |
Eisosomes | 0.35 | 9.53 | -1.25 | -1.51 | -6.75 | 1.77 | 7.23 | 4.62 | 1.88 | -6.56 | -0.75 | 11.93 | 0.11 | 0.83 | -13.00 |
Endoplasmic Reticulum | -0.65 | -1.98 | -4.04 | -3.98 | -3.77 | 2.13 | -2.79 | -1.95 | -4.84 | 1.56 | 0.62 | -2.82 | -4.85 | -5.92 | -3.31 |
Endosome | 2.13 | 3.51 | 2.40 | 1.39 | 0.97 | 2.09 | -6.22 | -1.82 | -5.55 | 5.55 | 4.54 | 0.23 | -0.25 | -3.99 | -0.44 |
Golgi | 18.19 | 11.71 | 45.42* | 28.01 | 34.84* | -3.39 | 10.39 | 19.21 | 33.14 | 5.05 | 13.77 | 12.07 | 53.42* | 39.61 | 36.29* |
Lipid Particles | -2.57 | -0.40 | -1.98 | -0.05 | -1.75 | 5.72 | 4.26 | 7.86 | 6.73 | 3.11 | 1.28 | 4.08 | 6.37 | 6.43 | 2.29 |
Mitochondria | 0.87 | 15.93 | 11.76 | 9.96 | 0.09 | -1.76 | 8.27 | 7.16 | 12.97 | -2.80 | -1.31 | 18.19 | 13.15 | 15.27 | -5.81 |
Mitotic Spindle | -7.01 | -11.18 | -6.57 | -5.49 | -3.96 | -2.98 | -6.38 | -12.25 | -10.74 | 1.42 | -8.24 | -12.75 | -14.58 | -11.42 | -3.17 |
None | -4.02 | 2.35 | -1.57 | -1.02 | -1.65 | 1.57 | 2.97 | 1.41 | -0.25 | -2.41 | -0.73 | 3.33 | -1.36 | -0.77 | -3.01 |
Nuclear Periphery | -5.92 | -4.32 | -7.40 | -7.01 | -3.03 | 3.11 | -2.00 | -4.35 | -6.92 | 1.03 | -1.39 | -4.64 | -9.11 | -8.89 | 0.39 |
Nuclear Periphery Foci | -3.99 | -4.25 | -6.05 | -5.77 | -1.64 | 3.89 | -2.96 | -0.38 | -4.10 | 2.84 | 0.82 | -4.65 | -5.83 | -6.15 | 2.76 |
Nucleolus | -2.80 | -0.30 | -3.62 | -2.25 | -3.59 | 2.53 | 1.98 | 2.17 | -3.67 | 0.41 | 1.55 | 2.21 | 0.10 | -3.56 | -5.56 |
Nucleus | -4.28 | -0.00 | -4.99 | -4.83 | -4.99 | 1.95 | 1.19 | -2.34 | -6.05 | -4.49 | -0.62 | 1.83 | -7.88 | -7.83 | -8.59 |
Peroxisomes | -2.79 | 9.46 | -3.95 | -3.11 | -6.02 | 2.59 | 0.53 | -4.13 | -7.93 | -3.39 | -2.67 | 8.66 | -11.30 | -9.86 | -16.51 |
Vacuole | -9.10 | -12.79 | -12.26 | -11.44 | -11.18 | -0.23 | -4.64 | -19.87 | -20.09 | -8.75 | -9.30 | -12.69 | -23.96 | -22.03 | -18.89 |
Vacuole Periphery | -2.44 | -6.90 | -0.43 | 0.43 | 2.15 | 1.72 | 1.45 | -0.24 | -1.61 | -1.48 | -0.79 | -5.54 | -1.54 | -1.19 | 1.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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