Standard name
Human Ortholog
Description GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.06 0.11 0.08 0.13 0.08 0.05
Bud 0.06 0 0.06 0.09 0.1 0.07 0.09 0.09 0.08 0.09 0.08 0.05 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.11 0.09 0 0
Bud Site 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.79 0.82 0.87 0.79 0.75 0.63 0.59 0.6 0.66 0.59 0.54 0.53 0.96 0.98 0.98 0.82 0.8 0.77 0.67 0.58 0.54 0.58 0.73 0.76
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.07 0.08 0.19 0.2 0.33 0.43 0.37 0.47 0.44 0.57 0.55 0 0 0 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 8 8 4 5 4 8 5 2 4 4 3 5 4 0 11 35 35 14 23 14 52 30 20
Bud 12 8 10 13 18 11 23 23 19 21 15 8 16 10 6 5 12 19 13 16 6 16 16 17
Bud Neck 0 0 6 4 3 1 5 3 6 2 2 1 0 2 0 3 15 6 14 20 21 36 7 9
Bud Site 4 7 2 5 5 8 10 5 4 8 2 7 2 0 0 3 4 6 – – – – – –
Cell Periphery 1 1 1 0 1 2 0 1 2 2 6 3 13 9 12 25 36 24 1 2 2 6 4 6
Cytoplasm 165 153 155 120 136 98 144 155 160 145 96 88 573 580 641 334 331 277 150 122 100 236 276 322
Endoplasmic Reticulum 4 9 0 0 1 1 2 0 0 2 1 2 0 1 1 18 28 20 1 0 0 2 0 0
Endosome 0 2 0 0 0 0 0 1 0 0 0 0 3 3 1 14 4 3 2 5 1 13 8 7
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 1 1 0 3 0
Mitochondria 44 14 15 28 36 52 105 95 114 107 101 92 0 0 1 6 2 5 8 8 18 16 10 16
Nucleus 0 0 0 0 6 0 2 6 1 1 1 1 0 1 0 2 4 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 1 0 2 0 0 1 0 1 1 0 1 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 1 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 3 0 0 1 1 2 1 0 4 1 2 6 2 3 13 2 4 0 0 0 0 0 0
SpindlePole 1 1 5 4 5 6 7 11 7 7 1 5 8 0 0 27 35 27 6 3 6 13 7 8
Vac/Vac Membrane 0 0 0 0 2 1 4 1 1 2 2 3 2 0 0 8 11 9 3 2 2 3 2 2
Unique Cell Count 208 187 179 151 181 156 245 260 244 244 177 166 596 594 652 405 412 358 224 212 187 408 377 423
Labelled Cell Count 244 206 203 179 220 185 314 309 316 308 232 216 629 612 668 470 519 437 224 212 187 408 377 423


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.3 3.4 3.2 3.8 3.5 3.2 3.6 3.3 3.2 3.1 3.0 5.0 4.8 4.9 5.9 6.9 7.1 4.2 4.2 4.8
Std Deviation (1e-4) 0.5 0.9 0.7 0.6 1.1 0.9 1.0 1.1 1.0 1.1 0.7 1.1 1.2 1.1 1.2 1.4 1.3 1.7 1.0 1.2 0.8
Intensity Change (Log2) – – – -0.08 0.16 0.02 -0.1 0.07 -0.05 -0.08 -0.13 -0.18 0.57 0.51 0.51 0.79 1.01 1.06 0.29 0.3 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4831 3.7012 3.8447 3.7378 4.4015 4.0211 2.4276 2.6429 2.6812 2.6551 2.4929 2.8233 3.346 3.2994 3.3737 3.0857 4.1126 3.5859
Actin 0.0887 0.0089 0.0344 0.0066 0.0159 0.029 0.0481 0.0054 0.0224 0.0115 0.0167 0.0278 0.0355 0.0125 0.071 0.036 0.0906 0.0265
Bud 0.0123 0.0136 0.0592 0.0241 0.007 0.0015 0.0109 0.0133 0.0462 0.0138 0.0053 0.0015 0.016 0.0241 0.0583 0.007 0.0003 0.0014
Bud Neck 0.021 0.012 0.0039 0.0014 0.0023 0.3545 0.0054 0.0042 0.0021 0.0051 0.0523 0.2728 0.053 0.002 0.0037 0.0003 0.0007 0.3001
Bud Periphery 0.0297 0.0219 0.1443 0.0438 0.029 0.0049 0.0452 0.0256 0.1404 0.0871 0.0119 0.007 0.0541 0.0257 0.1557 0.0188 0.0009 0.0032
Bud Site 0.0423 0.0995 0.0719 0.0049 0.0022 0.002 0.0577 0.1211 0.0086 0.0128 0.0289 0.0055 0.1154 0.0966 0.0434 0.0053 0.0003 0.0052
Cell Periphery 0.0068 0.0032 0.0041 0.0014 0.009 0.0022 0.0056 0.0038 0.0025 0.0033 0.0013 0.0037 0.0063 0.0038 0.005 0.0006 0.003 0.0016
Cytoplasm 0.2434 0.2973 0.1705 0.2737 0.2855 0.2533 0.2307 0.1602 0.1399 0.1359 0.3207 0.1762 0.2266 0.1463 0.1722 0.4417 0.3915 0.2324
Cytoplasmic Foci 0.0579 0.0146 0.0323 0.0306 0.0202 0.0723 0.0414 0.0156 0.0351 0.0201 0.0311 0.0449 0.0439 0.0144 0.0319 0.0063 0.0042 0.0695
Eisosomes 0.0023 0.0005 0.0008 0.0004 0.0007 0.0004 0.0013 0.0004 0.0012 0.0006 0.0009 0.0006 0.0012 0.0011 0.0017 0.0002 0.0032 0.0005
Endoplasmic Reticulum 0.0082 0.0017 0.0041 0.0033 0.0137 0.0012 0.002 0.0013 0.002 0.0033 0.0102 0.0022 0.0072 0.0013 0.0042 0.0046 0.0032 0.002
Endosome 0.0217 0.0013 0.0086 0.0091 0.0407 0.0075 0.0125 0.0007 0.0042 0.0431 0.0763 0.0072 0.011 0.0008 0.0133 0.0015 0.0039 0.0034
Golgi 0.0108 0.0006 0.0056 0.0023 0.0091 0.0038 0.0066 0.0004 0.0055 0.0171 0.0422 0.0032 0.0056 0.0004 0.0061 0.0009 0.0078 0.0013
Lipid Particles 0.0201 0.0005 0.0048 0.0026 0.0037 0.0114 0.0075 0.0003 0.0042 0.0053 0.0081 0.0076 0.0133 0.0004 0.0042 0.0004 0.0028 0.0008
Mitochondria 0.0213 0.002 0.0082 0.0028 0.0192 0.0037 0.0043 0.0005 0.0078 0.0062 0.0303 0.0074 0.0029 0.0014 0.0089 0.0009 0.0023 0.0005
None 0.3261 0.5107 0.4049 0.5595 0.4779 0.2333 0.487 0.6348 0.5396 0.6145 0.3342 0.4167 0.3596 0.6607 0.3832 0.4697 0.4808 0.3436
Nuclear Periphery 0.012 0.0024 0.0023 0.0014 0.0097 0.0026 0.0007 0.0003 0.001 0.0038 0.0096 0.0004 0.0035 0.0006 0.0017 0.0008 0.0012 0.0009
Nucleolus 0.0055 0.0006 0.001 0.0019 0.0035 0.0005 0.0054 0.001 0.0003 0.0008 0.0008 0.0004 0.0011 0.001 0.0009 0.0001 0.0001 0.0002
Nucleus 0.0204 0.0022 0.0032 0.0071 0.0211 0.0012 0.0038 0.0053 0.001 0.003 0.0041 0.0007 0.0024 0.002 0.0014 0.0007 0.0003 0.0009
Peroxisomes 0.0294 0.0008 0.0164 0.0077 0.0026 0.0088 0.0124 0.0009 0.0325 0.0039 0.0024 0.0088 0.0265 0.0008 0.0269 0.0023 0.001 0.0036
Punctate Nuclear 0.0145 0.0019 0.0133 0.0121 0.0038 0.0044 0.0063 0.0036 0.0019 0.0011 0.0017 0.0037 0.012 0.0026 0.0035 0.001 0.0005 0.0014
Vacuole 0.0044 0.0024 0.0051 0.0029 0.0173 0.001 0.0045 0.0013 0.0014 0.0056 0.0076 0.001 0.0025 0.0013 0.0022 0.0008 0.0011 0.0008
Vacuole Periphery 0.0013 0.0013 0.001 0.0004 0.0058 0.0004 0.0006 0.0001 0.0003 0.0021 0.0035 0.0004 0.0006 0.0001 0.0005 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9368 16.0061 12.3721 8.9144 20.313 17.1496 19.3868 12.0608 14.6106 14.3582
Translational Efficiency 0.8119 1.0028 0.4916 0.9771 0.3849 0.5602 0.6955 0.6389 0.37 0.5309

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
331 1468 331 807 567 1855 142 2605 898 3323 473 3412

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.61 686.86 836.31 834.90 697.85 715.58 944.41 943.37 680.07 702.89 868.76 917.71
Standard Deviation 70.65 116.73 81.81 107.28 72.73 85.26 107.15 119.79 75.64 101.39 102.89 125.71
Intensity Change Log 2 — 0.080442 0.364464 0.362030 — 0.036196 0.436496 0.434907 — 0.057697 0.402218 0.400232

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000287 0.001116 0.001379 0.003217 0.000359 0.002003 0.001959 0.000663 0.000332 0.001611 0.001553 0.001267
Bud Neck 0.116249 0.063172 0.046096 0.058045 0.072077 0.098809 0.020077 0.085522 0.088358 0.083066 0.038285 0.079023
Bud Site 0.015598 0.048208 0.040361 0.117998* 0.017316 0.066880 0.047497 0.097311 0.016683 0.058631 0.042503 0.102203*
Cell Periphery 0.000429 0.000230 0.000576 0.000482 0.000313 0.000309 0.000430 0.000371 0.000356 0.000274 0.000532 0.000397
Cytoplasm 0.421354* 0.430742* 0.257085* 0.353416* 0.467024* 0.420936* 0.336857* 0.571965* 0.450190* 0.425268* 0.281034* 0.520274*
Cytoplasmic Foci 0.178037 0.197293* 0.057598 0.033358 0.208796* 0.193638* 0.053427 0.006166 0.197459* 0.195253* 0.056346 0.012597
Eisosomes 0.000374 0.000235 0.000252 0.000174 0.000442 0.000494 0.000185 0.000061 0.000417 0.000380 0.000232 0.000088
Endoplasmic Reticulum 0.000350 0.001210 0.004493 0.002752 0.000646 0.000360 0.006267 0.000342 0.000536 0.000736 0.005026 0.000912
Endosome 0.005504 0.026033 0.007697 0.011810 0.007610 0.016542 0.008692 0.002068 0.006834 0.020735 0.007996 0.004372
Golgi 0.000712 0.006296 0.000297 0.002928 0.001884 0.007613 0.000798 0.000636 0.001452 0.007031 0.000448 0.001178
Lipid Particles 0.006606 0.005988 0.005480 0.001609 0.006288 0.003498 0.003758 0.000362 0.006405 0.004598 0.004963 0.000657
Mitochondria 0.004215 0.006635 0.000437 0.001596 0.002593 0.014278 0.000228 0.004076 0.003191 0.010902 0.000374 0.003489
Mitotic Spindle 0.001516 0.003722 0.000531 0.020908 0.000047 0.009135 0.009851 0.027570 0.000589 0.006744 0.003329 0.025994
None 0.003304 0.006328 0.003435 0.002206 0.001638 0.001100 0.001289 0.005568 0.002252 0.003409 0.002791 0.004773
Nuclear Periphery 0.000460 0.000354 0.001250 0.001159 0.000207 0.000118 0.000760 0.000428 0.000301 0.000223 0.001103 0.000601
Nuclear Periphery Foci 0.000731 0.000634 0.001614 0.002088 0.000159 0.000143 0.005223 0.000071 0.000370 0.000360 0.002697 0.000548
Nucleolus 0.001056 0.002012 0.001424 0.000608 0.000513 0.000598 0.000429 0.000805 0.000713 0.001223 0.001125 0.000758
Nucleus 0.208218* 0.074594 0.421224* 0.160256* 0.152940* 0.059508 0.211851* 0.130888* 0.173315* 0.066173 0.358368* 0.137834*
Peroxisomes 0.006931 0.021662 0.010129 0.008826 0.006805 0.028595 0.003111 0.001248 0.006851 0.025532 0.008022 0.003040
Vacuole 0.026420 0.100967 0.138367 0.211062* 0.051715 0.073926 0.287028* 0.062758 0.042391 0.085872 0.182997 0.097834
Vacuole Periphery 0.001649 0.002568 0.000274 0.005502 0.000628 0.001514 0.000282 0.001123 0.001004 0.001980 0.000276 0.002159

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.76 -3.60 -4.80 -2.63 -1.59 -7.33 -3.61 -1.89 6.00 3.06 -8.17 -5.04 -4.74 3.48 2.35
Bud Neck 3.55 4.77 5.52 6.07 0.78 -2.98 6.00 1.33 7.20 -7.43 0.74 6.37 4.40 7.51 -4.10
Bud Site -6.53 -3.12 -7.77 -3.29 -3.72 -11.61 -2.34 -11.23 0.92 -2.63 -13.05 -3.94 -13.78 -1.74 -4.71
Cell Periphery 4.33 -2.00 1.70 -3.34 3.89 0.12 -2.25 1.87 1.92 3.77 3.01 -3.79 2.89 0.18 6.14
Cytoplasm -0.58 9.44 5.95 9.09 -4.94 3.68 8.47 -5.27 -12.40 -12.28 2.53 14.46 -3.05 -7.69 -19.13
Cytoplasmic Foci -1.58 11.37 15.67 33.12 6.55 1.57 17.19 24.19 40.01 7.37 0.42 19.85 29.21 53.79 11.82
Eisosomes 3.67 2.81 6.23 3.21 4.72 -0.50 4.21 7.60 5.15 8.29 0.69 4.46 9.79 6.16 10.77
Endoplasmic Reticulum -4.17 -8.93 -13.48 -8.72 2.93 1.44 -8.97 0.46 -3.38 9.33 -1.29 -11.83 -5.31 -5.42 10.54
Endosome -11.94 -2.13 -0.58 11.39 1.47 -7.69 -0.10 8.72 15.77 7.32 -13.85 -1.27 7.33 20.48 8.18
Golgi -5.88 2.03 -0.77 4.77 -1.67 -7.19 3.26 4.26 9.98 0.38 -9.04 4.55 3.28 10.98 -1.31
Lipid Particles 0.49 0.49 2.57 6.83 7.40 3.69 3.01 8.72 7.78 8.65 2.19 1.28 6.80 11.08 11.31
Mitochondria -0.74 1.26 1.07 8.12 -2.69 -7.57 5.16 -0.93 7.34 -8.05 -5.29 2.46 0.48 8.96 -8.02
Mitotic Spindle -1.70 1.20 -5.04 -4.30 -5.50 -5.93 -1.13 -12.42 -5.86 -3.87 -6.11 -0.98 -13.09 -8.14 -9.64
None -2.32 0.35 1.07 3.57 0.78 1.51 2.52 -8.92 -9.25 -11.21 -2.00 -0.24 -5.67 -2.05 -4.34
Nuclear Periphery 0.67 -5.96 -5.82 -8.16 -0.61 3.88 -10.29 -14.50 -16.36 -1.58 1.26 -11.48 -12.50 -16.03 0.58
Nuclear Periphery Foci 0.30 -2.74 -2.59 -4.57 0.33 0.60 -9.27 4.01 3.05 9.39 0.08 -9.21 -0.85 -1.57 9.66
Nucleolus -2.44 -0.86 1.81 3.48 2.73 -1.07 1.34 -2.76 -1.38 -3.34 -2.85 -1.79 -0.56 2.57 1.43
Nucleus 11.68 -10.47 -0.15 -16.25 11.29 13.44 -3.57 -4.86 -28.62 1.57 17.39 -12.63 -3.15 -32.19 11.76
Peroxisomes -5.71 -0.96 1.24 8.42 1.98 -9.98 3.14 5.31 14.72 3.00 -11.37 -0.48 5.24 17.65 3.28
Vacuole -17.12 -13.32 -26.92 -18.71 -8.93 -5.81 -13.91 -13.69 -9.03 11.27 -14.71 -16.99 -26.87 -16.21 6.51
Vacuole Periphery -0.64 1.04 -0.28 0.34 -1.72 -4.56 4.28 -0.09 4.08 -3.13 -1.84 1.51 0.02 3.00 -2.68
Standard name
Human Ortholog
Description GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null mutant
Localization
Cell Percentages cytoplasm (20%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Msb4

Msb4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msb4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available