Standard name
Human Ortholog
Description Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.99 0.96 0.99 0.95 0.97 0.94 – 0.91 0.94 0.78 0.88 1.0 1.0 1.0 0.92 0.92 0.97 0.79 0.9 0.72 0.97 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0 0 0 0 – 0.1 0.1 0.34 0.12 0 0 0 0 0 0 0.07 0 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 2 5 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 2 0 9 – 6 3 11 4 0 0 0 0 0 0 0 0 0 0 2 2
Bud Neck 0 1 0 4 15 8 18 – 11 17 6 12 0 0 1 1 1 1 0 0 1 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 2 3 – 2 3 1 2 0 3 0 2 3 7 0 0 1 0 0 0
Cytoplasm 20 94 23 233 418 332 488 – 324 534 285 322 59 231 261 292 320 332 51 97 50 23 132 171
Endoplasmic Reticulum 0 1 1 0 2 1 3 – 0 0 2 2 0 0 1 18 18 24 0 0 1 0 0 0
Endosome 0 0 0 0 2 1 3 – 2 1 1 1 0 0 0 2 7 3 2 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 8 1 1 0 0 0 0 0 0
Mitochondria 3 0 0 0 0 4 19 – 37 56 123 44 0 0 0 11 8 2 4 3 9 0 3 1
Nucleus 0 0 0 3 2 1 3 – 1 4 0 3 0 1 0 1 0 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 5 0 1 – 4 11 6 8 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 0 0 0 2 9 2 3 – 1 2 0 3 0 0 0 14 24 14 3 0 0 0 0 2
Unique Cell Count 21 95 24 235 438 344 517 357 566 367 366 59 231 262 317 349 342 66 108 70 24 147 186
Labelled Cell Count 24 96 24 243 459 353 555 388 632 438 402 59 235 264 349 383 389 66 108 70 24 147 186


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.4 4.6 5.5 4.5 4.4 – 3.8 4.0 3.4 3.8 5.5 5.2 5.0 6.3 6.2 5.5 – 4.7 4.9
Std Deviation (1e-4) 0.5 0.6 0.7 1.4 1.6 1.1 1.4 – 1.1 1.3 0.6 1.5 1.4 0.9 1.2 1.5 1.7 1.4 – 1.2 1.3
Intensity Change (Log2) – – – 0.06 0.31 0.03 -0.01 – -0.22 -0.15 -0.39 -0.2 0.32 0.24 0.19 0.52 0.49 0.32 – 0.08 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.4871 -0.9606 -1.28 -1.3689 -2.0141 -0.8975 -1.2821 -0.7582 -0.628 -1.3328 -1.1287 -0.4395 -2.7877 -2.1476 -2.5084 -2.3695 -2.71 -2.5492
Actin 0.0164 0.0001 0.0152 0.0003 0.0001 0.0011 0.0158 0.001 0.006 0.0002 0.0003 0.0019 0.0074 0.0007 0.0002 0.0001 0 0.0006
Bud 0.0007 0.0005 0.0012 0.0001 0 0.0003 0.0034 0.0055 0.0021 0.0002 0 0.0033 0.0002 0 0.0001 0 0 0
Bud Neck 0.0031 0 0.0032 0.0001 0 0.0003 0.0005 0.0002 0.0013 0 0.0001 0.008 0.0001 0 0.0001 0 0.0001 0.0006
Bud Periphery 0.0018 0.0002 0.0039 0.0001 0 0.0004 0.003 0.0016 0.0014 0.0001 0 0.0044 0.0004 0.0001 0.0001 0.0001 0 0.0001
Bud Site 0.0084 0.0012 0.0074 0.0001 0 0.001 0.0032 0.0294 0.0112 0 0 0.0012 0.0003 0.0003 0.001 0.0001 0 0
Cell Periphery 0.0003 0.0001 0.0011 0 0 0.0001 0.0002 0.0007 0.0005 0 0.0002 0.0005 0.0001 0.0001 0 0 0 0
Cytoplasm 0.0664 0.095 0.0677 0.1611 0.0717 0.1187 0.0198 0.031 0.0443 0.0291 0.0147 0.0509 0.0292 0.0642 0.0578 0.0283 0.1145 0.0921
Cytoplasmic Foci 0.0169 0.0233 0.0108 0.0151 0.0041 0.009 0.0154 0.0195 0.0101 0.0061 0.0222 0.0149 0.0078 0.0047 0.0064 0.0209 0.0086 0.0148
Eisosomes 0.0021 0.0001 0.0006 0.0001 0.0001 0.0001 0.0003 0.0005 0.0004 0.0001 0.0003 0.0001 0.0007 0.0002 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0016 0.0011 0.0014 0.003 0.0006 0.0011 0.0007 0.001 0.0011 0.0007 0.0004 0.001 0.0008 0.0013 0.0009 0.0005 0.0005 0.0012
Endosome 0.0149 0.0047 0.0026 0.005 0.0012 0.0174 0.0009 0.0001 0.0035 0.0009 0.0001 0.0082 0.0016 0.0008 0.0009 0.0135 0.0018 0.002
Golgi 0.0159 0.0001 0.0022 0.0002 0 0.0068 0.0008 0.0001 0.0011 0.0001 0 0.0058 0.0007 0.0001 0.0001 0.0002 0 0.0001
Lipid Particles 0.0165 0.0018 0.0053 0.0007 0.0002 0.0107 0.0015 0.0003 0.0032 0.0007 0.0002 0.0013 0.0012 0.0007 0.0011 0.0005 0.0001 0.001
Mitochondria 0.0109 0.0006 0.0122 0.0004 0.0001 0.0345 0.0039 0.0008 0.0066 0.0003 0.0001 0.0179 0.0081 0.0002 0.0003 0.0009 0.0001 0.0002
None 0.8084 0.8629 0.8556 0.8088 0.9201 0.7884 0.9187 0.9019 0.8873 0.9555 0.9604 0.8689 0.9335 0.9226 0.9262 0.92 0.8696 0.8803
Nuclear Periphery 0.0006 0.0004 0.0006 0.0011 0.0003 0.0018 0.0014 0.0002 0.0041 0.0011 0.0001 0.0007 0.002 0.0007 0.001 0.0008 0.0003 0.0006
Nucleolus 0.0007 0.0005 0.0004 0.0001 0 0.0002 0.0011 0.0013 0.0017 0.0002 0.0001 0.0008 0.0009 0.0002 0.0004 0.0003 0.0001 0.0003
Nucleus 0.0013 0.0008 0.0006 0.0007 0.0002 0.0013 0.0026 0.0012 0.0061 0.0007 0.0001 0.0022 0.0006 0.0008 0.0008 0.0012 0.001 0.0018
Peroxisomes 0.0089 0.0003 0.0049 0.0003 0 0.001 0.0035 0.0002 0.003 0.0001 0.0003 0.0003 0.0022 0.0002 0.0002 0.0004 0.0001 0.0003
Punctate Nuclear 0.0012 0.0057 0.001 0.0009 0.0002 0.0006 0.0021 0.0027 0.0033 0.003 0.0001 0.0055 0.0014 0.0017 0.0016 0.0009 0.0027 0.0036
Vacuole 0.0026 0.0005 0.0017 0.0016 0.0009 0.0033 0.0007 0.0007 0.0014 0.0005 0.0001 0.0013 0.0004 0.0003 0.0004 0.0104 0.0004 0.0004
Vacuole Periphery 0.0004 0.0001 0.0005 0.0003 0.0001 0.0019 0.0003 0.0001 0.0005 0.0001 0 0.0009 0.0003 0.0001 0.0001 0.0009 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9792 9.5229 7.8103 13.3485 10.1771 11.2073 14.9497 14.1945 14.3725 15.7685
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1985 1667 2361 800 1602 1649 139 1843 3587 3316 2500 2643

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 570.86 648.61 796.59 738.43 630.28 645.63 887.95 948.26 597.40 647.13 801.67 884.75
Standard Deviation 71.69 98.87 83.27 140.16 62.67 85.20 85.14 125.64 73.97 92.34 85.96 162.01
Intensity Change Log 2 — 0.184214 0.480700 0.371324 — 0.034715 0.494486 0.589290 — 0.107701 0.487950 0.489791

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000194 0.000188 0.000815 0.000090 0.000331 0.000290 0.000100 0.000086 0.000262 0.000193 0.000316
Bud Neck 0.013305 0.032363 0.008968 0.007343 0.015577 0.043404 0.001287 0.039630 0.014320 0.037854 0.008541 0.029857
Bud Site 0.007586 0.010579 0.008120 0.035897 0.002543 0.016324 0.001771 0.027220 0.005334 0.013436 0.007767 0.029846
Cell Periphery 0.000375 0.000099 0.000087 0.000208 0.000107 0.000278 0.000186 0.000167 0.000255 0.000188 0.000093 0.000180
Cytoplasm 0.488745* 0.412610* 0.293962* 0.459796* 0.459108* 0.356848* 0.303381* 0.465923* 0.475509* 0.384880* 0.294485* 0.464069*
Cytoplasmic Foci 0.129372 0.220047* 0.003970 0.014308 0.235522* 0.217979* 0.021750 0.000689 0.176780 0.219019* 0.004958 0.004811
Eisosomes 0.000229 0.000102 0.000020 0.000043 0.000129 0.000159 0.000092 0.000012 0.000184 0.000130 0.000024 0.000022
Endoplasmic Reticulum 0.000906 0.000597 0.001996 0.002886 0.000659 0.000896 0.005250 0.000398 0.000796 0.000745 0.002177 0.001151
Endosome 0.003158 0.017348 0.001272 0.006990 0.009626 0.032699 0.003702 0.000364 0.006047 0.024982 0.001407 0.002370
Golgi 0.001056 0.003519 0.000281 0.003469 0.001899 0.005551 0.000074 0.000058 0.001433 0.004529 0.000270 0.001091
Lipid Particles 0.008723 0.007690 0.000536 0.001735 0.008473 0.015572 0.002740 0.000144 0.008612 0.011609 0.000659 0.000625
Mitochondria 0.002328 0.012765 0.001503 0.001363 0.005274 0.021306 0.000233 0.002094 0.003644 0.017013 0.001432 0.001873
Mitotic Spindle 0.001039 0.007201 0.005325 0.031706 0.002810 0.014168 0.000175 0.011334 0.001830 0.010665 0.005038 0.017501
None 0.015236 0.006560 0.004713 0.020322 0.002495 0.005733 0.004131 0.010895 0.009545 0.006149 0.004681 0.013748
Nuclear Periphery 0.000470 0.000664 0.002163 0.001234 0.000559 0.001001 0.001697 0.000894 0.000510 0.000831 0.002138 0.000997
Nuclear Periphery Foci 0.000287 0.000406 0.000344 0.001997 0.000341 0.000476 0.002255 0.000026 0.000311 0.000441 0.000450 0.000623
Nucleolus 0.002002 0.002965 0.000554 0.001070 0.001576 0.004082 0.000543 0.000760 0.001812 0.003520 0.000554 0.000854
Nucleus 0.303633* 0.184061* 0.639639* 0.342287* 0.207251* 0.162754* 0.540211* 0.422695* 0.260588* 0.173465* 0.634111* 0.398357*
Peroxisomes 0.001794 0.012771 0.000146 0.000987 0.007193 0.019809 0.000303 0.000090 0.004205 0.016271 0.000154 0.000361
Vacuole 0.018942 0.065476 0.025975 0.064442 0.037645 0.077257 0.109783 0.016144 0.027295 0.071334 0.030635 0.030763
Vacuole Periphery 0.000731 0.001984 0.000238 0.001102 0.001123 0.003373 0.000146 0.000361 0.000906 0.002675 0.000233 0.000586

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.61 -3.54 -5.57 -4.61 -4.58 -4.51 -5.94 -2.18 3.97 5.48 -6.02 -4.45 -5.88 -1.07 -2.32
Bud Neck -7.77 2.69 1.62 9.01 -1.05 -9.92 11.19 -5.57 4.35 -12.98 -12.63 4.55 -5.39 6.35 -8.52
Bud Site -1.74 0.76 -4.16 -3.45 -4.63 -10.31 -1.07 -7.85 0.31 -4.41 -7.31 -0.80 -7.31 -2.49 -6.35
Cell Periphery 9.49 10.09 6.36 -7.26 -8.91 -3.85 -4.69 -0.03 3.70 4.08 2.58 10.00 6.70 2.38 -3.99
Cytoplasm 9.37 32.48 16.59 8.79 -4.35 11.09 10.15 8.96 -2.72 -6.42 14.80 34.94 18.09 3.69 -14.47
Cytoplasmic Foci -14.39 36.68 33.30 38.56 -6.46 2.32 32.85 42.94 42.09 4.76 -8.65 53.26 53.62 57.80 1.23
Eisosomes 9.38 15.55 11.41 5.81 -14.68 -3.97 0.32 23.37 23.63 9.11 6.43 19.94 19.56 26.34 -1.81
Endoplasmic Reticulum 2.25 -11.41 -14.53 -16.44 -8.60 -1.82 -8.25 -1.37 0.97 8.12 0.49 -15.85 -11.28 -12.94 5.12
Endosome -13.69 5.49 -1.14 10.38 -3.41 -11.91 5.77 12.39 18.36 8.26 -17.37 11.37 10.16 22.46 0.10
Golgi -5.80 3.65 -0.26 3.22 -1.71 -6.10 8.70 8.86 9.80 2.18 -8.42 7.10 4.27 10.83 -1.19
Lipid Particles 1.02 10.66 8.78 9.96 -8.36 -5.49 6.84 14.22 13.23 6.97 -3.73 15.83 15.85 16.80 0.21
Mitochondria -8.19 3.16 4.43 9.26 0.73 -8.31 6.80 4.70 10.95 -13.50 -11.65 5.87 5.63 14.13 -1.59
Mitotic Spindle -3.94 -4.57 -6.58 -4.76 -5.15 -5.13 2.01 -4.70 1.61 -7.25 -6.59 -3.69 -8.64 -2.55 -5.30
None 4.95 7.14 1.49 -3.17 -5.02 -3.78 -0.59 -23.73* -7.42 -11.47 3.20 5.69 -3.28 -7.71 -12.42
Nuclear Periphery -2.17 -24.29 -17.28 -14.34 0.82 -2.96 -10.74 -14.04 -7.07 -0.43 -3.70 -27.53 -20.35 -13.45 4.44
Nuclear Periphery Foci -1.96 -3.21 -6.10 -5.84 -5.70 -1.57 -6.70 3.90 4.81 7.37 -2.47 -5.11 -4.92 -3.95 -2.90
Nucleolus -2.33 3.01 1.25 3.81 -2.22 -4.61 3.79 -0.50 4.15 -4.03 -4.92 3.65 0.33 5.16 -3.22
Nucleus 16.92 -48.20 -10.19 -20.31 20.12 6.66 -11.99 -39.68 -44.60 -0.50 17.54 -60.29 -34.89 -48.30 21.48
Peroxisomes -10.82 11.84 6.25 11.97 -3.48 -7.12 8.32 8.79 12.54 3.26 -12.04 10.82 10.59 17.17 -1.50
Vacuole -17.74 -10.04 -18.66 -11.88 -15.57 -12.15 -8.27 4.14 14.90 8.74 -21.27 -9.05 -14.33 0.73 -5.87
Vacuole Periphery -4.89 3.44 1.16 5.71 -1.54 -5.44 5.16 5.10 8.56 -0.36 -7.29 5.98 4.82 11.04 -1.50
Standard name
Human Ortholog
Description Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (15%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Skm1

Skm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available