Standard name
Human Ortholog
Description Catalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain; another complex member is Trm112p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.12 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.97 0.94 0.82 0.78 0.83 0.69 0.69 0.64 0.59 1.0 0.99 0.99 0.96 0.95 0.96 0.97 0.97 0.92 0.96 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.08 0.26 0.34 0.19 0.41 0.44 0.53 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0 0.06 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1
Bud 1 0 0 0 3 2 6 6 6 8 7 7 1 0 0 0 2 0 0 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 9 34 7 4 7 12 12 6 9 7 8 13 14 13 23 20 20 0 0 0 0 0 0
Cytoplasm 180 464 289 128 174 196 256 334 177 255 162 171 502 428 483 281 309 268 178 460 263 384 392 426
Endoplasmic Reticulum 0 2 2 0 0 2 0 1 0 1 1 3 1 4 4 16 10 11 0 2 4 2 3 2
Endosome 0 0 1 0 0 3 1 2 0 0 1 0 0 1 0 0 1 0 0 3 3 3 3 8
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 1 1 1
Mitochondria 2 0 3 6 14 61 111 76 106 163 134 149 1 0 0 3 3 3 0 0 1 1 3 2
Nucleus 1 2 1 0 0 2 1 3 2 2 1 1 2 4 3 5 3 1 0 0 0 0 2 1
Nuclear Periphery 1 0 0 0 0 1 1 1 1 2 6 5 0 0 0 0 0 1 0 0 0 0 1 4
Nucleolus 0 0 0 0 0 1 0 1 1 5 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
SpindlePole 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 1 3 3 3 7 15 14 24 20 21 16 6 0 0 0 4 10 1 1 0 0 0 2 2
Unique Cell Count 184 469 293 132 186 239 328 401 257 372 255 292 504 433 488 293 324 280 184 473 288 399 418 464
Labelled Cell Count 189 481 334 144 202 291 402 460 319 467 336 350 520 452 503 332 359 307 184 473 288 399 418 464


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 10.0 10.4 8.4 7.7 6.6 5.9 6.2 5.5 5.7 5.1 4.7 10.1 9.8 9.0 18.0 16.5 18.4 11.2 10.9 10.8
Std Deviation (1e-4) 0.9 1.4 3.9 2.4 2.6 2.3 2.0 1.9 1.1 2.6 1.8 0.9 1.1 1.0 1.0 4.1 3.8 4.3 1.3 1.5 1.6
Intensity Change (Log2) – – – -0.29 -0.43 -0.64 -0.8 -0.74 -0.92 -0.87 -1.01 -1.13 -0.04 -0.08 -0.2 0.8 0.67 0.83 0.12 0.07 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.0 -3.7 -3.7 -3.8 -4.5 -4.2 -4.8 -3.9 0 -5.2 -4.5 -5.1 -1.5 -2.4 -1.8
Cytoplasm -1.2 -3.0 -6.7 -7.8 -6.6 -9.7 -10.0 -10.7 -11.8 1.5 0.3 0.4 -2.0 -2.3 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 2.2 2.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.7 8.6 10.5 7.3 11.8 12.7 13.9 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.3 2.5 3.3 4.0 3.2 3.4 0 0 0 0 0 1.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1169 6.0918 6.0091 5.5181 5.515 5.7739 3.4913 5.1839 4.1695 4.04 3.7433 4.8094 4.4552 5.603 4.8926 5.4105 5.0634 5.0044
Actin 0.0188 0.0007 0.0052 0.0086 0.0052 0.0003 0.0684 0.0014 0.0295 0.0685 0.0368 0.002 0.0184 0.0009 0.0015 0.0001 0.0001 0.0049
Bud 0.0007 0.0001 0.0003 0.0003 0.0003 0.0001 0.005 0.0005 0.0013 0.0009 0.0008 0.0003 0.0005 0.011 0.0038 0.0001 0.0001 0.0024
Bud Neck 0.0019 0.0001 0.001 0.0004 0.001 0.0005 0.0153 0.0003 0.0023 0.001 0.0018 0.0019 0.0015 0.0002 0.0004 0.0004 0.0002 0.0009
Bud Periphery 0.0004 0 0.0003 0.0001 0.0003 0 0.0068 0.0002 0.0011 0.001 0.0007 0.0003 0.0006 0.0031 0.0013 0 0 0.001
Bud Site 0.0099 0.0005 0.0007 0.0019 0.0002 0 0.0083 0.0062 0.0054 0.014 0.005 0.0002 0.0031 0.0049 0.0027 0 0 0.0003
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0001 0 0.0008 0.0002 0.0002 0.0003 0.0002 0 0.0003 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.8818 0.9502 0.9535 0.9333 0.8937 0.9541 0.7217 0.968 0.8458 0.8457 0.7248 0.9526 0.8562 0.9471 0.9475 0.982 0.978 0.9498
Cytoplasmic Foci 0.0117 0.0031 0.0056 0.0208 0.0161 0.0038 0.0245 0.0045 0.0193 0.009 0.047 0.0064 0.0244 0.0052 0.0084 0.0036 0.0052 0.0087
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0 0.0004 0 0.0001 0.0002 0.0002 0 0.0002 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0072 0.0023 0.0021 0.0022 0.0025 0.0028 0.014 0.0031 0.0041 0.0012 0.0082 0.0014 0.0067 0.0037 0.0017 0.002 0.0029 0.0028
Endosome 0.007 0.0019 0.0039 0.0075 0.0105 0.0026 0.029 0.0029 0.0274 0.0101 0.0667 0.0095 0.0272 0.0045 0.0053 0.003 0.006 0.0086
Golgi 0.0015 0.0002 0.0009 0.0021 0.0028 0.0002 0.009 0.0004 0.0104 0.0046 0.0421 0.0045 0.005 0.0003 0.0013 0.0001 0.0002 0.0023
Lipid Particles 0.0012 0.0001 0.0005 0.0018 0.0003 0.0001 0.0123 0.0002 0.0067 0.0039 0.0363 0.0086 0.0093 0.0001 0.0035 0 0 0.0012
Mitochondria 0.0006 0.0001 0.0007 0.0008 0.001 0.0001 0.0071 0.0001 0.0048 0.0181 0.0067 0.0052 0.0027 0.0005 0.0012 0.0001 0.0002 0.0006
None 0.0318 0.0381 0.0192 0.0052 0.0628 0.0256 0.0187 0.0096 0.0199 0.0034 0.0016 0.003 0.0204 0.0113 0.0152 0.0055 0.0035 0.0074
Nuclear Periphery 0.0095 0.0005 0.0009 0.0016 0.0005 0.0069 0.02 0.0004 0.0024 0.0007 0.0016 0.0005 0.0051 0.002 0.0006 0.0003 0.0008 0.002
Nucleolus 0.0001 0 0.0001 0.0002 0 0 0.0027 0 0.0003 0.0007 0.0006 0 0.0002 0.0002 0.0001 0 0 0.0002
Nucleus 0.0049 0.0007 0.0013 0.0014 0.0009 0.0016 0.011 0.0008 0.0023 0.0014 0.001 0.0012 0.0035 0.0023 0.0017 0.0011 0.0014 0.0025
Peroxisomes 0.007 0 0.0003 0.0032 0.0006 0.0001 0.0099 0.0001 0.007 0.0111 0.0111 0.0011 0.0079 0.0001 0.0014 0 0 0.0005
Punctate Nuclear 0.0016 0.0002 0.002 0.0077 0.0003 0.0002 0.0063 0.0002 0.0032 0.0003 0.002 0.0005 0.0039 0.0006 0.0011 0.0001 0.0002 0.003
Vacuole 0.0016 0.0008 0.0011 0.0008 0.0005 0.0005 0.0061 0.0006 0.0057 0.0033 0.0038 0.0005 0.002 0.0014 0.001 0.0013 0.001 0.0007
Vacuole Periphery 0.0004 0.0001 0.0002 0.0002 0.0002 0.0003 0.0026 0.0001 0.001 0.0007 0.0012 0.0002 0.0007 0.0004 0.0002 0.0003 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.2377 37.8793 41.5999 27.1431 31.8646 13.552 30.8313 22.7477 16.192 32.7104
Translational Efficiency 1.5285 1.4302 0.9296 0.8161 0.9019 1.7569 1.2339 0.9187 1.1143 0.9651

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1668 664 989 1925 1885 2067 1095 1345 3553 2731 2084 3270

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 841.27 1043.99 1191.71 1436.85 858.06 992.28 1265.69 1465.33 850.18 1004.85 1230.58 1448.56
Standard Deviation 108.58 141.43 159.26 198.85 113.21 134.54 174.25 203.09 111.38 138.04 171.33 201.10
Intensity Change Log 2 — 0.311467 0.502392 0.772269 — 0.209669 0.560774 0.772075 — 0.260963 0.532167 0.772171

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000173 0.000333 0.000175 0.002207 0.000185 0.000241 0.000359 0.000415 0.000179 0.000263 0.000272 0.001470
Bud Neck 0.002157 0.001527 0.000644 0.001593 0.002474 0.001766 0.001028 0.001397 0.002326 0.001708 0.000846 0.001513
Bud Site 0.009479 0.019181 0.005264 0.014575 0.010283 0.012043 0.006659 0.012639 0.009905 0.013779 0.005997 0.013779
Cell Periphery 0.000069 0.000093 0.000041 0.000068 0.000147 0.000056 0.000097 0.000105 0.000111 0.000065 0.000070 0.000083
Cytoplasm 0.712116* 0.771394* 0.821213* 0.826612* 0.677360* 0.794651* 0.796029* 0.850830* 0.693677* 0.788996* 0.807981* 0.836573*
Cytoplasmic Foci 0.162285 0.129180 0.010533 0.014940 0.201373* 0.101138 0.019646 0.022972 0.183023* 0.107956 0.015321 0.018243
Eisosomes 0.000024 0.000013 0.000004 0.000005 0.000049 0.000014 0.000011 0.000004 0.000037 0.000014 0.000008 0.000005
Endoplasmic Reticulum 0.001560 0.001177 0.000220 0.000198 0.004688 0.000491 0.000605 0.000086 0.003219 0.000658 0.000422 0.000152
Endosome 0.010121 0.009307 0.000477 0.002551 0.009574 0.006211 0.003122 0.002521 0.009831 0.006964 0.001867 0.002539
Golgi 0.004593 0.002705 0.000092 0.002275 0.008757 0.003406 0.001588 0.002786 0.006802 0.003236 0.000878 0.002485
Lipid Particles 0.001546 0.001010 0.000284 0.000787 0.002861 0.000629 0.000766 0.000809 0.002243 0.000721 0.000537 0.000796
Mitochondria 0.002616 0.000228 0.000108 0.000878 0.001826 0.000503 0.000489 0.000454 0.002197 0.000436 0.000308 0.000704
Mitotic Spindle 0.000914 0.001255 0.001226 0.037138 0.002216 0.007338 0.008666 0.018801 0.001605 0.005859 0.005136 0.029596
None 0.001918 0.000982 0.005174 0.001860 0.003585 0.002472 0.004523 0.004201 0.002802 0.002110 0.004832 0.002823
Nuclear Periphery 0.000069 0.000067 0.000151 0.000150 0.000094 0.000046 0.000255 0.000104 0.000082 0.000051 0.000206 0.000131
Nuclear Periphery Foci 0.000709 0.002138 0.001745 0.001547 0.000831 0.000840 0.006423 0.002517 0.000774 0.001156 0.004203 0.001946
Nucleolus 0.000260 0.000148 0.000066 0.000145 0.000252 0.000167 0.000096 0.000100 0.000256 0.000162 0.000082 0.000126
Nucleus 0.020633 0.013671 0.126993 0.047602 0.024193 0.017989 0.077535 0.042473 0.022522 0.016939 0.101007 0.045493
Peroxisomes 0.000885 0.001286 0.000081 0.000782 0.000951 0.001553 0.000504 0.000657 0.000920 0.001488 0.000303 0.000731
Vacuole 0.065504 0.042790 0.025457 0.041549 0.044890 0.047208 0.070870 0.035752 0.054567 0.046134 0.049318 0.039165
Vacuole Periphery 0.002370 0.001516 0.000051 0.002538 0.003410 0.001237 0.000728 0.000377 0.002922 0.001305 0.000407 0.001649

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.47 -1.05 -2.62 -1.99 -2.37 -1.21 -4.88 -3.26 -1.75 1.71 -2.44 -4.06 -2.96 -2.40 -2.01
Bud Neck 2.46 7.40 4.29 0.40 -4.07 3.05 5.66 3.90 0.59 -1.28 3.53 9.10 6.00 1.20 -3.45
Bud Site -4.65 2.42 0.93 5.02 -1.75 -1.53 3.33 0.21 1.51 -2.53 -4.01 3.80 1.04 4.49 -2.69
Cell Periphery -2.96 3.99 -0.81 2.37 -4.51 15.63 5.19 5.69 -9.90 0.36 11.32 6.39 5.35 -5.93 -1.17
Cytoplasm -5.38 -8.02 -6.86 0.46 1.72 -16.11 -4.35 -12.02 2.92 -6.39 -16.23 -8.48 -13.54 3.59 -3.89
Cytoplasmic Foci 4.67 34.80 34.56 19.11 -1.68 19.85 42.04 39.50 23.16 -4.06 19.02 54.24 53.06 32.47 -3.13
Eisosomes 4.40 8.22 8.59 7.19 1.10 12.12 12.24 15.57 8.23 11.91 12.13 14.66 17.60 10.70 10.45
Endoplasmic Reticulum 0.90 4.41 4.92 2.80 3.45 6.16 6.03 6.85 2.97 11.14 6.41 7.17 8.09 3.85 9.98
Endosome 0.75 15.65 14.45 7.38 -2.70 5.14 13.72 16.88 9.10 3.03 4.99 20.81 22.21 12.02 1.75
Golgi 3.45 14.26 10.46 4.02 -2.68 6.83 10.88 11.58 4.10 -0.16 6.70 15.19 15.31 5.48 -0.99
Lipid Particles 1.58 5.08 0.18 -1.70 -8.97 10.39 8.33 4.66 -8.31 -5.02 9.04 9.62 3.78 -7.67 -9.09
Mitochondria 2.63 2.76 2.57 -0.32 -1.38 4.39 4.87 5.64 3.25 0.55 3.87 4.41 4.34 2.20 -0.36
Mitotic Spindle -0.69 -1.52 -6.75 -6.68 -6.05 -3.07 -3.02 -4.73 -2.22 -1.82 -3.47 -3.35 -8.11 -4.63 -5.13
None 2.41 -6.39 -0.26 -5.59 9.24 1.43 -0.23 0.97 -0.84 2.21 1.37 -3.35 1.04 -0.76 7.98
Nuclear Periphery -0.09 -10.58 -12.26 -8.83 0.04 3.26 -14.53 -6.47 -12.97 10.17 2.85 -17.80 -12.96 -18.17 7.57
Nuclear Periphery Foci -6.17 -6.57 -10.25 0.52 0.94 -0.18 -12.31 -13.57 -13.45 5.78 -4.36 -13.74 -16.57 -11.45 6.26
Nucleolus 2.44 4.97 2.90 -0.16 -8.16 2.20 5.43 4.31 1.17 -1.21 2.94 7.30 4.96 0.77 -5.86
Nucleus 3.98 -27.45 -19.43 -21.97 17.22 5.39 -22.30 -13.21 -16.41 11.67 5.74 -34.74 -23.61 -27.84 19.99
Peroxisomes -1.80 12.26 6.41 4.33 -3.33 -1.99 4.43 3.77 3.87 -0.83 -2.50 8.65 6.79 5.10 -2.01
Vacuole 5.09 2.98 -13.08 -16.13 -13.86 -1.73 -15.99 -15.74 -14.56 3.67 3.19 -11.38 -20.91 -22.70 -4.88
Vacuole Periphery 1.95 10.57 4.61 1.62 -2.03 4.43 8.35 10.80 2.74 0.89 4.48 12.31 9.56 2.32 -1.18
Standard name
Human Ortholog
Description Catalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain; another complex member is Trm112p
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Trm11

Trm11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available