Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar membrane protein of unknown function; involved in vacuolar protein sorting; also detected in the mitochondria |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 0 | 3 | 7 | – | 5 | 10 | 7 | 9 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud | 1 | 0 | 0 | 0 | 0 | 4 | 5 | – | 3 | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 4 | – | 5 | 6 | 3 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 13 | 11 | 12 | 17 | 17 | 29 | – | 24 | 26 | 20 | 22 | 1 | 5 | 6 | 2 | 4 | 1 | 0 | 1 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 1 |
Endosome | 39 | 45 | 93 | 61 | 41 | 31 | 56 | – | 38 | 35 | 20 | 14 | 100 | 112 | 107 | 3 | 2 | 3 | 58 | 115 | 102 | 81 | 139 | 154 |
Golgi | 4 | 5 | 4 | 10 | 23 | 18 | 109 | – | 55 | 61 | 38 | 36 | 12 | 17 | 23 | 5 | 22 | 23 | 10 | 4 | 6 | 7 | 12 | 25 |
Mitochondria | 35 | 50 | 7 | 11 | 13 | 108 | 32 | – | 99 | 46 | 62 | 43 | 27 | 23 | 10 | 1 | 4 | 4 | 5 | 8 | 8 | 4 | 7 | 6 |
Nucleus | 6 | 11 | 8 | 1 | 10 | 4 | 2 | – | 4 | 6 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 7 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 9 | 12 | 5 | 2 | 1 | 1 | 4 | – | 2 | 2 | 1 | 3 | 6 | 4 | 6 | 3 | 7 | 2 | 7 | 8 | 17 | 3 | 8 | 8 |
Nucleolus | 30 | 41 | 52 | 18 | 34 | 62 | 69 | – | 64 | 52 | 44 | 28 | 47 | 65 | 56 | 5 | 13 | 6 | 3 | 5 | 4 | 1 | 2 | 2 |
Peroxisomes | 1 | 0 | 0 | 0 | 5 | 2 | 6 | – | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 5 | 12 | 25 | 11 | 26 | 8 | 31 | – | 5 | 7 | 6 | 2 | 4 | 13 | 11 | 2 | 1 | 0 | 3 | 6 | 5 | 3 | 1 | 3 |
Vac/Vac Membrane | 133 | 267 | 156 | 64 | 88 | 46 | 128 | – | 73 | 84 | 62 | 56 | 141 | 165 | 205 | 41 | 104 | 69 | 109 | 222 | 107 | 46 | 70 | 94 |
Unique Cell Count | 187 | 355 | 245 | 135 | 190 | 223 | 349 | 279 | 246 | 197 | 162 | 230 | 270 | 295 | 47 | 113 | 78 | 207 | 387 | 263 | 156 | 248 | 302 | |
Labelled Cell Count | 270 | 458 | 361 | 192 | 259 | 304 | 483 | 381 | 342 | 275 | 229 | 339 | 404 | 424 | 64 | 161 | 112 | 207 | 387 | 263 | 156 | 248 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | 9.5 | 9.5 | 10.0 | 14.9 | 9.9 | 14.5 | – | 13.6 | 13.8 | 12.9 | 13.7 | 12.0 | 13.8 | 14.8 | 21.4 | 25.2 | 24.7 | 9.0 | 11.0 | 12.9 |
Std Deviation (1e-4) | 2.8 | 2.5 | 1.9 | 2.6 | 6.6 | 3.3 | 3.3 | – | 3.9 | 4.1 | 4.5 | 5.1 | 2.8 | 3.2 | 3.7 | 7.0 | 7.6 | 5.9 | 2.6 | 3.3 | 3.8 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.66 | 0.06 | 0.61 | – | 0.53 | 0.54 | 0.44 | 0.53 | 0.34 | 0.55 | 0.64 | 1.18 | 1.41 | 1.38 | -0.08 | 0.21 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | 1.9 | 1.4 | 1.8 | – | 1.9 | 2.6 | 2.3 | 3.3 | -2.8 | -1.7 | -1.6 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.4 | -3.7 | -5.9 | -6.1 | – | -6.4 | -6.0 | -6.7 | -6.6 | 1.2 | 0.8 | -0.4 | -4.2 | -7.2 | -5.7 |
Golgi | 0 | 4.5 | 3.3 | 9.0 | – | 6.5 | 7.6 | 6.3 | 6.8 | 2.2 | 2.7 | 3.3 | 0 | 6.0 | 7.7 |
Mitochondria | 2.3 | 2.0 | 11.4 | 3.1 | – | 9.3 | 5.7 | 8.2 | 7.1 | 3.8 | 2.7 | 0.4 | 0 | 0 | 0 |
Nucleus | 0 | 1.0 | -1.0 | 0 | – | -1.4 | -0.6 | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.9 | -0.9 | 1.7 | -0.4 | – | 0.5 | 0 | 0.3 | -1.0 | -0.2 | 0.8 | -0.6 | -1.7 | -2.2 | -2.7 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.7 | 1.1 | -2.8 | -0.5 | – | -4.1 | -3.3 | -2.9 | -3.6 | -3.9 | -2.3 | -3.0 | 0 | -3.2 | -2.9 |
Vacuole | -3.1 | -3.6 | -9.4 | -6.5 | – | -8.6 | -6.5 | -6.7 | -5.8 | -0.5 | -0.6 | 1.4 | 3.2 | 5.6 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6786 | 5.5707 | 5.1414 | 4.9895 | 4.4129 | 5.424 | 7.0119 | 8.3059 | 8.3091 | 7.2961 | 7.4894 | 7.9155 | 9.4777 | 11.1915 | 9.6862 | 9.7637 | 9.7937 | 10.5641 |
Actin | 0.0003 | 0 | 0.0038 | 0.0116 | 0.0323 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0387 | 0 | 0.0076 | 0.0002 | 0.0012 | 0 |
Bud | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Neck | 0.0002 | 0.0002 | 0.0003 | 0.0014 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0016 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Bud Periphery | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 |
Bud Site | 0.0002 | 0.0003 | 0.0003 | 0.001 | 0.0014 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0027 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0005 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.0043 | 0.0023 | 0.0019 | 0.001 | 0.0012 | 0.0015 | 0.001 | 0.002 | 0.0013 | 0.0007 | 0.0007 | 0.0007 | 0.0008 | 0.0009 | 0.0007 | 0.0004 | 0.0021 | 0.0004 |
Cytoplasmic Foci | 0.0178 | 0.0355 | 0.0284 | 0.0352 | 0.0246 | 0.0189 | 0.0284 | 0.0422 | 0.0304 | 0.0178 | 0.0358 | 0.0219 | 0.0256 | 0.0327 | 0.0206 | 0.0363 | 0.0417 | 0.019 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0021 | 0.0006 | 0.0012 | 0.0003 | 0.0013 | 0.0013 | 0.0006 | 0.0006 | 0.0011 | 0.0003 | 0.0006 | 0.0006 | 0.0023 | 0.0004 | 0.001 | 0.0004 | 0.0025 | 0.0005 |
Endosome | 0.4047 | 0.3793 | 0.3574 | 0.3704 | 0.4533 | 0.3696 | 0.4846 | 0.3832 | 0.4787 | 0.5283 | 0.4105 | 0.4473 | 0.3397 | 0.3507 | 0.3902 | 0.3457 | 0.4683 | 0.3493 |
Golgi | 0.0353 | 0.0325 | 0.0463 | 0.0498 | 0.0599 | 0.0445 | 0.0412 | 0.0325 | 0.0675 | 0.0359 | 0.0519 | 0.0676 | 0.0437 | 0.0385 | 0.0599 | 0.0317 | 0.0379 | 0.0423 |
Lipid Particles | 0.0514 | 0.0551 | 0.066 | 0.036 | 0.0225 | 0.0668 | 0.0755 | 0.0709 | 0.07 | 0.0223 | 0.0596 | 0.0721 | 0.1534 | 0.0602 | 0.0814 | 0.0286 | 0.0267 | 0.0959 |
Mitochondria | 0.0139 | 0.0025 | 0.0122 | 0.0155 | 0.0081 | 0.0039 | 0.0081 | 0.0057 | 0.0153 | 0.0018 | 0.0023 | 0.0051 | 0.0109 | 0.0018 | 0.0248 | 0.002 | 0.0074 | 0.0069 |
None | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0164 | 0.0068 | 0.0046 | 0.0009 | 0.0045 | 0.0154 | 0.0037 | 0.0028 | 0.0062 | 0.0013 | 0.0093 | 0.007 | 0.0207 | 0.0024 | 0.0189 | 0.0025 | 0.0096 | 0.0052 |
Nucleolus | 0.0044 | 0.0024 | 0.0031 | 0.0003 | 0.0004 | 0.0022 | 0.0068 | 0.0032 | 0.0008 | 0.0003 | 0.0067 | 0.0011 | 0.0014 | 0.0015 | 0.0014 | 0.0003 | 0.0004 | 0.0007 |
Nucleus | 0.0031 | 0.0017 | 0.0026 | 0.0003 | 0.0008 | 0.0024 | 0.0013 | 0.0009 | 0.0005 | 0.0003 | 0.0005 | 0.0007 | 0.0011 | 0.0008 | 0.0012 | 0.0003 | 0.0007 | 0.0004 |
Peroxisomes | 0.0011 | 0.0018 | 0.0036 | 0.0131 | 0.0118 | 0.0027 | 0.0026 | 0.0017 | 0.0038 | 0.0004 | 0.0012 | 0.0014 | 0.0087 | 0.0049 | 0.0044 | 0.0044 | 0.0015 | 0.0025 |
Punctate Nuclear | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0037 | 0.0008 | 0.0052 | 0.0003 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0011 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0001 |
Vacuole | 0.1986 | 0.2405 | 0.2123 | 0.2416 | 0.1649 | 0.1685 | 0.1605 | 0.2231 | 0.1627 | 0.1685 | 0.1998 | 0.1474 | 0.1383 | 0.2144 | 0.1515 | 0.21 | 0.129 | 0.1473 |
Vacuole Periphery | 0.2444 | 0.2376 | 0.2537 | 0.2208 | 0.2065 | 0.2998 | 0.1791 | 0.2295 | 0.1599 | 0.2207 | 0.2204 | 0.2259 | 0.2063 | 0.2898 | 0.2336 | 0.3362 | 0.2694 | 0.3288 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 78.5306 | 55.0949 | 60.6648 | 103.3286 | 50.2647 | 63.5845 | 68.7345 | 74.5897 | 84.5179 | 100.7304 |
Translational Efficiency | 1.4796 | 1.4562 | 1.5445 | 0.933 | 1.6359 | 2.4428 | 1.6158 | 1.6224 | 1.6359 | 1.3054 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1747 | 198 | 171 | 17 | 1327 | 2240 | 2116 | 607 | 3074 | 2438 | 2287 | 624 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 873.32 | 1109.79 | 1575.68 | 807.24 | 902.44 | 986.07 | 1430.70 | 1381.90 | 885.89 | 996.12 | 1441.54 | 1366.24 |
Standard Deviation | 132.94 | 198.51 | 292.80 | 704.34 | 155.71 | 166.56 | 293.91 | 335.93 | 143.94 | 172.71 | 296.29 | 363.38 |
Intensity Change Log 2 | — | 0.345704 | 0.851392 | -0.113513 | — | 0.127859 | 0.664818 | 0.614750 | — | 0.239106 | 0.759591 | 0.301928 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000029 | 0.000141 | 0.000425 | 0.000092 | 0.000053 | 0.000046 | 0.000140 | 0.000592 | 0.000039 | 0.000054 | 0.000161 | 0.000578 |
Bud Neck | 0.001937 | 0.001912 | 0.005562 | 0.000320 | 0.002469 | 0.001713 | 0.001465 | 0.001486 | 0.002167 | 0.001729 | 0.001772 | 0.001455 |
Bud Site | 0.009367 | 0.011039 | 0.002247 | 0.000799 | 0.008028 | 0.006424 | 0.001879 | 0.004722 | 0.008789 | 0.006799 | 0.001906 | 0.004615 |
Cell Periphery | 0.000149 | 0.000217 | 0.000188 | 0.000272 | 0.000155 | 0.000164 | 0.000389 | 0.000572 | 0.000152 | 0.000168 | 0.000374 | 0.000564 |
Cytoplasm | 0.008254 | 0.002650 | 0.000285 | 0.000150 | 0.005962 | 0.003271 | 0.000533 | 0.001525 | 0.007265 | 0.003221 | 0.000514 | 0.001487 |
Cytoplasmic Foci | 0.035471 | 0.029303 | 0.024174 | 0.010014 | 0.042772 | 0.030421 | 0.020531 | 0.028832 | 0.038622 | 0.030331 | 0.020803 | 0.028319 |
Eisosomes | 0.000008 | 0.000008 | 0.000042 | 0.000470 | 0.000013 | 0.000011 | 0.000029 | 0.000036 | 0.000010 | 0.000011 | 0.000030 | 0.000048 |
Endoplasmic Reticulum | 0.000469 | 0.001068 | 0.000615 | 0.000069 | 0.000672 | 0.000698 | 0.000273 | 0.000984 | 0.000556 | 0.000728 | 0.000299 | 0.000959 |
Endosome | 0.273684* | 0.187611 | 0.029782 | 0.020672 | 0.315262* | 0.219327* | 0.099377 | 0.148192 | 0.291633* | 0.216752* | 0.094173 | 0.144718 |
Golgi | 0.055207 | 0.080539 | 0.049284 | 0.158730* | 0.067533 | 0.074553 | 0.100494 | 0.235222* | 0.060528 | 0.075039 | 0.096665 | 0.233138* |
Lipid Particles | 0.000473 | 0.000920 | 0.009912 | 0.000798 | 0.001728 | 0.001415 | 0.014280 | 0.014961 | 0.001015 | 0.001375 | 0.013953 | 0.014575 |
Mitochondria | 0.011652 | 0.006465 | 0.050017 | 0.000547 | 0.017720 | 0.026246 | 0.044915 | 0.029902 | 0.014271 | 0.024639 | 0.045296 | 0.029103 |
Mitotic Spindle | 0.002625 | 0.007442 | 0.009562 | 0.002189 | 0.002142 | 0.003443 | 0.001397 | 0.003516 | 0.002416 | 0.003768 | 0.002007 | 0.003480 |
None | 0.000245 | 0.000089 | 0.000540 | 0.648148* | 0.000264 | 0.000506 | 0.000437 | 0.000260 | 0.000253 | 0.000472 | 0.000445 | 0.017911 |
Nuclear Periphery | 0.000160 | 0.001796 | 0.017680 | 0.000367 | 0.000425 | 0.000779 | 0.003534 | 0.002616 | 0.000274 | 0.000861 | 0.004592 | 0.002555 |
Nuclear Periphery Foci | 0.000955 | 0.005961 | 0.019459 | 0.001312 | 0.001426 | 0.001862 | 0.007567 | 0.009605 | 0.001158 | 0.002195 | 0.008456 | 0.009379 |
Nucleolus | 0.000493 | 0.000797 | 0.002570 | 0.000026 | 0.001048 | 0.001147 | 0.001329 | 0.000416 | 0.000732 | 0.001119 | 0.001422 | 0.000406 |
Nucleus | 0.000606 | 0.004354 | 0.006173 | 0.000011 | 0.001078 | 0.000910 | 0.000305 | 0.000311 | 0.000809 | 0.001189 | 0.000744 | 0.000303 |
Peroxisomes | 0.001460 | 0.001821 | 0.001458 | 0.000185 | 0.004546 | 0.002014 | 0.001002 | 0.001155 | 0.002792 | 0.001998 | 0.001036 | 0.001129 |
Vacuole | 0.310055* | 0.349440* | 0.360108* | 0.102608 | 0.273240* | 0.303646* | 0.367691* | 0.290312* | 0.294163* | 0.307366* | 0.367124* | 0.285198* |
Vacuole Periphery | 0.286701* | 0.306427* | 0.409917* | 0.052223 | 0.253463* | 0.321405* | 0.332435* | 0.224782* | 0.272353* | 0.320188* | 0.338228* | 0.220081* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.15 | -4.63 | -1.86 | 0.83 | 3.69 | 0.49 | -2.59 | -4.32 | -4.38 | -3.51 | -1.46 | -3.98 | -4.45 | -4.32 | -3.33 |
Bud Neck | 0.02 | -1.98 | 4.75 | 2.08 | 2.81 | 1.51 | 2.24 | 2.15 | 0.81 | -0.11 | 1.22 | 1.13 | 2.36 | 1.01 | 1.31 |
Bud Site | -0.55 | 6.29 | 9.10 | 3.27 | 1.84 | 1.30 | 6.04 | 2.55 | 1.62 | -3.82 | 2.11 | 10.47 | 4.19 | 2.14 | -3.72 |
Cell Periphery | -0.85 | -1.55 | -2.21 | -0.59 | -1.55 | -0.34 | -5.85 | -5.58 | -5.36 | -2.28 | -0.68 | -6.22 | -5.73 | -5.34 | -2.45 |
Cytoplasm | 4.32 | 8.24 | 8.38 | 2.93 | 1.48 | 2.26 | 4.94 | 2.91 | 1.25 | -1.50 | 4.56 | 9.14 | 4.77 | 1.31 | -1.51 |
Cytoplasmic Foci | 1.47 | 2.60 | 3.84 | 2.55 | 1.84 | 4.89 | 9.11 | 4.29 | 0.60 | -3.11 | 4.75 | 10.84 | 3.86 | 0.77 | -2.89 |
Eisosomes | -0.11 | -4.23 | -2.31 | -2.31 | -2.13 | 1.28 | -7.07 | -6.52 | -7.02 | -1.73 | -0.96 | -9.45 | -5.46 | -5.31 | -2.48 |
Endoplasmic Reticulum | -1.74 | -0.73 | 6.37 | 2.91 | 2.51 | -0.21 | 3.60 | -1.71 | -1.62 | -4.56 | -1.72 | 4.01 | -2.58 | -1.34 | -4.33 |
Endosome | 4.91 | 29.69* | 18.92 | 8.32 | 0.71 | 10.27 | 25.40 | 15.52 | 7.89 | -5.95 | 10.69 | 33.33 | 16.65 | 8.22 | -6.34 |
Golgi | -2.55 | 0.73 | -1.58 | -1.18 | -1.66 | -1.69 | -7.09 | -14.99 | -14.48 | -11.91 | -4.44 | -9.51 | -16.01 | -14.45 | -12.28 |
Lipid Particles | -1.15 | -4.20 | -0.74 | 0.19 | 3.98 | 0.49 | -9.37 | -5.32 | -5.50 | -0.25 | -0.89 | -10.99 | -5.71 | -5.51 | -0.24 |
Mitochondria | 1.74 | -3.53 | 8.60 | 2.15 | 4.57 | -3.14 | -8.31 | -3.08 | -0.94 | 3.54 | -5.14 | -11.27 | -4.24 | -1.19 | 3.92 |
Mitotic Spindle | -1.32 | -1.54 | 0.23 | 1.33 | 1.54 | -2.14 | 1.70 | -1.44 | -0.10 | -2.30 | -1.99 | 0.66 | -1.13 | 0.24 | -1.56 |
None | 3.60 | -3.61 | -5.41 | -5.42 | -5.41 | -1.18 | -2.89 | 0.08 | 1.25 | 5.68 | -1.19 | -4.97 | -3.34 | -3.30 | -3.31 |
Nuclear Periphery | -1.20 | -4.00 | -1.20 | 1.04 | 3.95 | -2.93 | -9.32 | -5.07 | -4.19 | 1.71 | -3.97 | -9.54 | -5.48 | -3.85 | 3.32 |
Nuclear Periphery Foci | -1.39 | -3.74 | -0.49 | 1.28 | 3.65 | -1.05 | -6.77 | -3.80 | -3.63 | -0.95 | -2.39 | -8.23 | -3.96 | -3.43 | -0.47 |
Nucleolus | -0.70 | -3.12 | 2.62 | 2.00 | 3.96 | -0.54 | -1.00 | 2.56 | 2.98 | 4.34 | -1.67 | -3.06 | 1.92 | 3.09 | 5.02 |
Nucleus | -0.92 | -1.05 | 3.56 | 1.07 | 1.15 | 0.48 | 3.19 | 2.66 | 2.40 | -0.18 | -1.00 | 0.08 | 2.36 | 2.18 | 0.98 |
Peroxisomes | -0.47 | 0.00 | 6.92 | 2.13 | 3.01 | 3.46 | 6.08 | 5.63 | 1.96 | -0.78 | 1.55 | 6.22 | 5.21 | 2.11 | -0.48 |
Vacuole | -2.32 | -2.98 | 4.81 | 5.39 | 5.63 | -4.29 | -13.31 | -1.68 | 1.29 | 7.82 | -2.36 | -13.06 | 0.87 | 2.22 | 8.40 |
Vacuole Periphery | -1.02 | -5.89 | 10.67 | 9.09 | 12.29 | -7.84 | -9.49 | 2.65 | 9.47 | 10.85 | -6.94 | -10.02 | 5.48 | 10.05 | 12.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|