Standard name
Human Ortholog
Description Protein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.06
Bud 0 0 0 0.05 0 0.06 0.05 0 – 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.42 0.52 0.4 0.51 0.62 0.55 0.51 0.47 – 0.33 0.26 0.21 0.67 0.66 0.71 0.65 0.46 0.45 0.13 0.14 0.06 0.05 0.08 0.09
Endoplasmic Reticulum 0.05 0.1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0.24 0.07 0.22 0.18 0.18 0.07 0 0 – 0 0 0 0.11 0.14 0.16 0.14 0.3 0.3 0.25 0.25 0.27 0.2 0.17 0.19
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.08 0.13 0.11 0.1 0.13
Mitochondria 0.25 0.33 0.31 0.3 0.2 0.52 0.61 0.59 – 0.82 0.86 0.86 0 0 0 0.15 0.08 0.08 0.34 0.3 0.32 0.44 0.4 0.36
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0 0.06 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0.07 0.05 0 0
SpindlePole 0 0 0.06 0 0 0 0 0 – 0 0 0 0.06 0 0 0.1 0.14 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.05 0 0 0 – 0 0 0 0.05 0 0.07 0 0.07 0.14 0.11 0.08 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 18 5 1 0 0 0 0 – 0 0 0 13 13 6 3 7 6 4 22 22 11 25 34
Bud 2 5 12 13 6 23 26 27 – 20 27 29 2 2 1 1 3 4 2 6 2 1 6 10
Bud Neck 0 1 0 0 1 0 0 1 – 0 0 0 5 7 0 3 0 0 1 8 0 2 5 5
Bud Site 0 1 1 0 0 6 2 1 – 7 6 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 1 – 2 2 0 3 3 2 2 2 4 0 0 0 0 0 0
Cytoplasm 38 205 120 130 185 231 246 317 – 171 127 110 208 208 248 181 117 90 22 74 23 21 41 53
Endoplasmic Reticulum 5 40 6 3 3 0 1 0 – 8 3 6 11 11 8 9 18 13 1 6 7 5 13 10
Endosome 22 27 67 46 54 28 12 18 – 1 0 4 35 43 55 39 77 61 42 134 115 78 86 116
Golgi 0 1 2 1 0 0 0 0 – 0 0 0 3 3 2 0 0 1 11 41 54 43 52 79
Mitochondria 23 131 93 76 60 217 295 400 – 430 416 448 9 9 3 41 20 16 58 161 138 171 207 216
Nucleus 4 0 0 2 3 0 3 2 – 1 0 1 0 0 2 6 2 2 0 0 0 0 1 0
Nuclear Periphery 2 0 0 0 1 0 2 2 – 4 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Nucleolus 3 0 0 0 2 0 5 5 – 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 27 11 17 5 2 7 – 2 1 0 16 14 5 0 2 5 3 9 30 18 15 14
SpindlePole 4 10 18 11 11 15 20 17 – 13 10 16 18 13 15 28 35 18 2 18 17 15 18 15
Vac/Vac Membrane 6 10 12 10 14 8 15 16 – 13 16 11 16 13 23 11 19 28 18 43 8 14 29 30
Unique Cell Count 91 397 303 257 297 417 484 675 526 481 520 310 316 351 280 256 200 173 541 427 389 515 599
Labelled Cell Count 110 449 364 304 357 533 630 814 673 609 630 339 339 370 324 302 249 173 541 427 389 515 599


Early Golgi, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.8 4.4 4.3 4.3 3.3 3.2 3.4 – 2.9 3.0 3.1 6.0 6.0 6.1 5.0 5.8 6.4 6.0 6.1 6.2
Std Deviation (1e-4) 0.7 0.8 1.0 1.2 1.8 1.1 1.1 0.8 – 0.8 0.6 0.6 1.1 1.1 1.0 1.0 1.2 1.6 1.1 1.4 1.5
Intensity Change (Log2) – – – -0.03 -0.01 -0.42 -0.46 -0.34 – -0.6 -0.52 -0.49 0.47 0.47 0.49 0.2 0.41 0.56 0.46 0.48 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0 1.8 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.21 1.2747 1.497 0.9947 1.3903 1.0403 2.3141 2.7368 2.459 2.3467 2.2197 2.6498 3.1015 2.4554 2.5851 2.5863 2.6325 2.4274
Actin 0.0224 0.0012 0.027 0.0073 0.0025 0.001 0.0392 0.0115 0.0018 0.0045 0.0054 0.0062 0.0341 0.0036 0.0141 0.0009 0.0004 0.0024
Bud 0.0007 0.0014 0.0008 0.0014 0.002 0.0004 0.0007 0.0011 0.0005 0.0001 0.0013 0.0004 0.0002 0.0002 0.0019 0.0004 0.0001 0.0002
Bud Neck 0.0016 0.0006 0.0042 0.0016 0.0022 0.0018 0.0016 0.0008 0.0003 0.0013 0.0018 0.0025 0.001 0.0002 0.0002 0.0003 0.0001 0.0039
Bud Periphery 0.0011 0.0029 0.0013 0.0027 0.0048 0.001 0.002 0.0008 0.0009 0.0001 0.0077 0.0012 0.0002 0.0003 0.0034 0.0006 0.0001 0.0002
Bud Site 0.0037 0.007 0.0086 0.0035 0.0077 0.0004 0.0008 0.0172 0.0011 0.0011 0.0011 0.0003 0.0009 0.001 0.0017 0.0015 0.0001 0.0003
Cell Periphery 0.0005 0.0008 0.0006 0.0018 0.0004 0.0002 0.0003 0.0005 0.0004 0.0001 0.0007 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.0888 0.1448 0.1123 0.1116 0.0385 0.1247 0.0649 0.1468 0.1074 0.0191 0.0555 0.1667 0.1084 0.1939 0.1356 0.1252 0.0858 0.2246
Cytoplasmic Foci 0.1012 0.0978 0.1211 0.1438 0.213 0.1209 0.1548 0.1733 0.1351 0.2189 0.1458 0.1837 0.1602 0.0837 0.1186 0.1422 0.1145 0.1256
Eisosomes 0.0006 0.0003 0.0007 0.0006 0.0001 0.0001 0.0007 0.0002 0.0006 0.0001 0.0003 0.0002 0.0003 0.0006 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0169 0.0085 0.0055 0.0091 0.002 0.0022 0.0077 0.0061 0.0037 0.001 0.003 0.0127 0.0046 0.009 0.0045 0.0074 0.0079 0.0085
Endosome 0.0854 0.1439 0.135 0.164 0.2756 0.138 0.1433 0.1867 0.1741 0.2444 0.2212 0.1378 0.1157 0.1098 0.1141 0.2697 0.1578 0.1615
Golgi 0.0087 0.012 0.0111 0.0206 0.0409 0.0192 0.0218 0.0204 0.0429 0.0293 0.0204 0.0163 0.0259 0.0103 0.0316 0.0342 0.0045 0.0283
Lipid Particles 0.0167 0.0207 0.0206 0.0231 0.0223 0.0209 0.0497 0.0183 0.0365 0.0239 0.053 0.0252 0.0412 0.0086 0.0263 0.0215 0.0174 0.0219
Mitochondria 0.0092 0.0135 0.0178 0.1039 0.033 0.0392 0.0183 0.0022 0.0113 0.0028 0.0091 0.0067 0.0042 0.0062 0.0048 0.0173 0.002 0.009
None 0.5477 0.4985 0.451 0.3578 0.2897 0.4921 0.4371 0.3837 0.4557 0.3301 0.4085 0.412 0.3961 0.5539 0.5184 0.36 0.5958 0.3916
Nuclear Periphery 0.0199 0.0034 0.0022 0.0077 0.0019 0.0012 0.0123 0.0015 0.002 0.001 0.0113 0.0013 0.0132 0.0023 0.0011 0.0022 0.0017 0.0041
Nucleolus 0.0014 0.0013 0.002 0.0018 0.0065 0.0004 0.0004 0.0007 0.0004 0.0008 0.0029 0.0002 0.0009 0.0002 0.0003 0.0001 0.0001 0.0002
Nucleus 0.0153 0.0027 0.0022 0.003 0.0031 0.0011 0.0054 0.0017 0.0012 0.0007 0.0036 0.0006 0.0578 0.0009 0.0006 0.0008 0.0005 0.0011
Peroxisomes 0.0062 0.0084 0.0262 0.0167 0.0294 0.0219 0.0283 0.0107 0.0163 0.0773 0.0246 0.0169 0.0135 0.0068 0.0178 0.0028 0.0041 0.0058
Punctate Nuclear 0.0186 0.0024 0.0124 0.0031 0.0046 0.0015 0.0026 0.0081 0.0018 0.0392 0.0127 0.0016 0.0155 0.0012 0.0012 0.0054 0.001 0.0034
Vacuole 0.0285 0.0251 0.0337 0.0113 0.0162 0.0088 0.0056 0.0066 0.0045 0.0033 0.0073 0.0058 0.0048 0.0057 0.0028 0.0043 0.0044 0.0055
Vacuole Periphery 0.005 0.0029 0.0036 0.0034 0.0037 0.0031 0.0025 0.0011 0.0016 0.0009 0.0029 0.0016 0.0009 0.0012 0.0007 0.0029 0.0016 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3398 7.9227 10.7781 15.3275 11.0121 6.6433 9.6795 11.9397 12.9352 13.7821
Translational Efficiency 1.3433 1.4259 1.2726 0.8693 0.9761 1.9244 1.1771 1.0646 1.0892 0.9865

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
126 1432 1506 1203 1979 1443 1789 1517 2105 2875 3295 2720

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 918.48 767.74 753.57 845.05 705.37 738.17 776.59 842.29 718.13 752.90 766.07 843.51
Standard Deviation 130.28 103.60 110.11 145.94 77.26 92.18 129.37 140.13 95.83 99.14 121.49 142.74
Intensity Change Log 2 — -0.258630 -0.285507 -0.120212 — 0.065573 0.138773 0.255937 — -0.108783 -0.085736 0.055332

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005596 0.000833 0.005851 0.004806 0.000262 0.000852 0.004802 0.011895 0.000581 0.000843 0.005282 0.008760
Bud Neck 0.008490 0.014045 0.010477 0.012950 0.019321 0.029480 0.012765 0.012047 0.018672 0.021792 0.011719 0.012446
Bud Site 0.044599 0.013521 0.023360 0.034645 0.005607 0.012364 0.016546 0.031069 0.007941 0.012940 0.019660 0.032650
Cell Periphery 0.000114 0.000088 0.000170 0.000181 0.000114 0.000190 0.000125 0.000142 0.000114 0.000139 0.000146 0.000159
Cytoplasm 0.046720 0.243809* 0.239333* 0.155006* 0.116257* 0.202290* 0.224180* 0.160208* 0.112095* 0.222970* 0.231106* 0.157908*
Cytoplasmic Foci 0.242252* 0.428901* 0.196317* 0.210042* 0.398070* 0.393785* 0.240516* 0.255144* 0.388744* 0.411276* 0.220315* 0.235196*
Eisosomes 0.000116 0.000131 0.000093 0.000105 0.000339 0.000389 0.000095 0.000112 0.000325 0.000260 0.000094 0.000109
Endoplasmic Reticulum 0.001598 0.000982 0.008079 0.001469 0.003949 0.000869 0.003714 0.001428 0.003808 0.000925 0.005709 0.001446
Endosome 0.258044* 0.054571 0.050747 0.144101 0.096677 0.082780 0.079161 0.138909 0.106336 0.068729 0.066174 0.141205
Golgi 0.041469 0.067233 0.024687 0.053039 0.123834* 0.063039 0.028485 0.077774 0.118903* 0.065128 0.026749 0.066834
Lipid Particles 0.012818 0.013272 0.022629 0.017343 0.057160 0.024394 0.025598 0.013570 0.054506 0.018854 0.024241 0.015239
Mitochondria 0.008259 0.024514 0.006884 0.009035 0.079433 0.044912 0.006035 0.008003 0.075172 0.034752 0.006423 0.008459
Mitotic Spindle 0.031026 0.019629 0.141756* 0.107448* 0.003483 0.005597 0.123074* 0.065168 0.005131 0.012586 0.131613* 0.083867
None 0.000072 0.003402 0.003332 0.001472 0.001167 0.001008 0.002916 0.003439 0.001101 0.002200 0.003106 0.002569
Nuclear Periphery 0.000505 0.000279 0.002856 0.001245 0.000461 0.000312 0.002050 0.000638 0.000464 0.000295 0.002419 0.000906
Nuclear Periphery Foci 0.002622 0.000841 0.014030 0.003221 0.000295 0.000232 0.007056 0.002973 0.000435 0.000535 0.010244 0.003083
Nucleolus 0.000794 0.001248 0.003511 0.002283 0.000927 0.000695 0.003307 0.001869 0.000919 0.000970 0.003401 0.002052
Nucleus 0.010003 0.032669 0.145984* 0.053069 0.016682 0.029654 0.077937 0.035914 0.016282 0.031156 0.109038 0.043501
Peroxisomes 0.085561 0.025055 0.028239 0.046729 0.014294 0.035827 0.049411 0.069489 0.018560 0.030461 0.039734 0.059423
Vacuole 0.188875 0.050783 0.068824 0.135840 0.052975 0.065803 0.090700 0.107321 0.061110 0.058322 0.080702 0.119935
Vacuole Periphery 0.010466 0.004194 0.002840 0.005969 0.008694 0.005530 0.001525 0.002889 0.008800 0.004864 0.002126 0.004251

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.04 0.03 1.16 -4.91 2.07 -4.61 -9.67 -6.40 -5.98 -3.56 -1.44 -10.34 -7.50 -7.17 -2.31
Bud Neck -2.58 -1.40 -2.38 0.17 -1.96 -4.32 3.56 4.21 7.49 0.62 -1.96 4.63 3.94 6.39 -0.78
Bud Site 3.27 2.20 1.31 -5.81 -2.46 -5.41 -8.81 -11.22 -7.14 -5.62 -4.43 -9.27 -12.01 -9.09 -5.48
Cell Periphery 1.92 -3.75 -1.67 -3.53 1.17 -2.67 -1.10 -1.23 1.89 -0.59 -1.63 -3.95 -2.16 -0.14 0.53
Cytoplasm -16.29 -16.17 -11.55 5.49 5.02 -11.69 -15.60 -8.76 2.21 5.56 -18.37 -20.79 -11.91 5.62 7.51
Cytoplasmic Foci -10.87 3.40 4.00 29.93 1.39 0.58 27.03 24.41 20.81 -1.73 -3.34 32.43 31.13 35.28 -0.54
Eisosomes -0.28 1.71 1.55 2.70 0.13 -1.10 9.92 9.31 7.22 -1.63 2.19 9.96 9.51 7.65 -0.89
Endoplasmic Reticulum 2.08 -11.98 0.19 -3.28 14.02 5.54 -0.82 4.16 -3.72 9.00 5.50 -4.83 4.14 -4.77 16.48
Endosome 8.66 9.02 6.06 -9.67 -11.13 3.35 7.80 -3.85 -6.33 -9.86 10.25 13.73 -1.62 -11.55 -14.84
Golgi -3.14 1.98 0.16 6.40 -4.39 11.61 23.50 11.48 0.26 -10.19 11.88 24.06 14.90 4.10 -10.82
Lipid Particles 0.03 -2.41 -0.22 -0.50 3.81 11.47 12.37 16.92 5.90 5.67 14.69 13.02 16.86 4.14 6.76
Mitochondria -5.07 0.35 0.26 8.31 -0.14 8.54 24.19 23.20 13.24 -1.66 12.37 23.92 23.05 15.64 -1.15
Mitotic Spindle 0.90 -7.88 -4.91 -11.28 4.37 -1.68 -21.08* -14.21 -13.37 7.91 -5.19 -28.36 -19.30 -16.85 8.65
None -4.01 -8.51 -5.92 2.17 3.58 0.43 -2.83 -2.00 -2.25 -0.34 -2.23 -4.46 -2.24 -0.14 0.94
Nuclear Periphery 1.17 -9.25 -5.14 -8.16 4.29 1.34 -11.05 -3.96 -6.26 8.39 1.71 -14.77 -6.88 -10.30 8.66
Nuclear Periphery Foci 2.36 -8.05 -0.20 -2.93 8.22 1.66 -11.77 -4.19 -4.29 5.28 -0.43 -15.87 -5.42 -4.99 9.72
Nucleolus -1.90 -6.58 -3.80 -2.56 2.08 2.23 -6.88 -2.60 -3.29 3.48 -0.91 -9.72 -4.52 -3.94 4.01
Nucleus -5.01 -20.60 -10.44 -7.88 12.06 -6.18 -18.96 -10.54 -5.66 9.17 -8.95 -29.18 -15.97 -9.50 15.11
Peroxisomes 4.71 4.56 3.71 -3.84 -3.13 -10.03 -11.35 -14.00 -6.35 -4.29 -7.16 -8.90 -13.00 -7.69 -5.39
Vacuole 6.96 5.56 0.15 -20.21 -15.69 -3.46 -15.22 -16.84 -13.85 -4.93 0.92 -11.43 -22.22 -24.05 -14.17
Vacuole Periphery 1.87 2.43 1.79 -0.11 -2.10 6.31 19.34 12.79 6.55 -3.24 8.78 16.60 10.22 3.68 -3.32
Standard name
Human Ortholog
Description Protein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
Localization
Cell Percentages mitochondrion (16%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Bsc6

Bsc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bsc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available