Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of SEACAT, a subcomplex of the SEA complex; Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; null suppresses cdc13-1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 0 | 0 | 2 |
Bud | 0 | 1 | 5 | 1 | 0 | 6 | 8 | 9 | 10 | – | 7 | 5 | 3 | 2 | 7 | 2 | 0 | 4 | 0 | 2 | 2 | 2 | 2 | 5 |
Bud Neck | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 113 | 224 | 277 | 215 | 255 | 264 | 339 | 468 | 232 | – | 111 | 144 | 144 | 209 | 153 | 280 | 194 | 302 | 10 | 38 | 45 | 14 | 10 | 27 |
Endoplasmic Reticulum | 1 | 2 | 0 | 1 | 0 | 6 | 0 | 4 | 0 | – | 0 | 1 | 1 | 4 | 2 | 21 | 14 | 22 | 2 | 1 | 5 | 1 | 3 | 3 |
Endosome | 120 | 48 | 20 | 0 | 34 | 5 | 6 | 6 | 3 | – | 0 | 0 | 25 | 19 | 1 | 48 | 36 | 52 | 180 | 93 | 93 | 48 | 53 | 88 |
Golgi | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 1 | 1 | 1 | 0 |
Mitochondria | 4 | 30 | 51 | 88 | 44 | 127 | 169 | 194 | 163 | – | 93 | 139 | 9 | 19 | 36 | 19 | 6 | 20 | 36 | 41 | 65 | 12 | 19 | 22 |
Nucleus | 3 | 8 | 1 | 4 | 0 | 2 | 3 | 4 | 2 | – | 1 | 4 | 0 | 1 | 3 | 3 | 2 | 7 | 1 | 3 | 1 | 2 | 1 | 1 |
Nuclear Periphery | 3 | 7 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 3 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 13 | 3 | 4 | 1 | 5 | 4 |
Nucleolus | 7 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 2 | 0 | 5 | 0 | 1 | 4 | 1 | 0 | 3 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 32 | 9 | 6 | 2 | 3 | 3 | 3 | 5 | 0 | – | 0 | 2 | 0 | 1 | 2 | 7 | 8 | 3 | 12 | 9 | 3 | 6 | 9 | 15 |
Vac/Vac Membrane | 212 | 216 | 80 | 31 | 69 | 25 | 26 | 20 | 15 | – | 11 | 21 | 95 | 95 | 26 | 160 | 118 | 166 | 194 | 299 | 256 | 144 | 93 | 158 |
Unique Cell Count | 397 | 447 | 401 | 284 | 340 | 359 | 430 | 581 | 312 | 166 | 235 | 242 | 305 | 194 | 458 | 309 | 481 | 470 | 504 | 490 | 246 | 208 | 341 | |
Labelled Cell Count | 499 | 549 | 443 | 342 | 406 | 440 | 556 | 713 | 427 | 226 | 319 | 277 | 354 | 235 | 548 | 381 | 579 | 470 | 504 | 490 | 246 | 208 | 341 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 4.3 | 3.8 | 3.7 | 4.2 | 3.7 | 3.5 | 3.5 | 3.4 | – | 3.3 | 3.2 | 4.9 | 4.1 | 3.1 | 6.1 | 6.9 | 6.3 | 4.7 | 4.9 | 5.2 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.8 | 1.2 | 1.7 | 1.3 | 1.1 | 1.4 | 1.6 | – | 1.3 | 1.3 | 2.4 | 1.2 | 1.0 | 1.4 | 2.0 | 1.8 | 1.4 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.17 | -0.03 | -0.12 | -0.09 | -0.15 | – | -0.18 | -0.21 | 0.39 | 0.14 | -0.27 | 0.69 | 0.89 | 0.74 | 0.34 | 0.37 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.9 | 5.4 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9406 | 3.4232 | 3.1666 | 3.1676 | 3.9172 | 3.2181 | -1.6175 | -0.7393 | -0.8805 | -1.0852 | -1.1594 | -0.8308 | 3.5202 | 3.7092 | 3.0752 | 3.936 | 3.1506 | 3.4628 |
Actin | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0019 | 0.0001 | 0.017 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0017 |
Bud | 0.0004 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0004 | 0.0006 | 0.0046 | 0.0041 | 0.0007 | 0.0047 | 0.0008 | 0.0005 | 0.0016 | 0.0007 | 0.0008 | 0.0024 | 0.0018 |
Bud Neck | 0.0003 | 0.0005 | 0.0107 | 0.0003 | 0.0006 | 0.0019 | 0.0059 | 0.0016 | 0.0017 | 0.0005 | 0.0007 | 0.0048 | 0.0021 | 0.0002 | 0.0014 | 0.0008 | 0.0003 | 0.0031 |
Bud Periphery | 0.0011 | 0.0004 | 0.0011 | 0.0002 | 0.0005 | 0.0007 | 0.0008 | 0.0047 | 0.0045 | 0.0007 | 0.0057 | 0.0008 | 0.0008 | 0.0016 | 0.0008 | 0.0018 | 0.0079 | 0.0053 |
Bud Site | 0.0009 | 0.0062 | 0.0049 | 0.0002 | 0.0007 | 0.0008 | 0.0018 | 0.0145 | 0.0065 | 0.0004 | 0.0007 | 0.0009 | 0.0019 | 0.0013 | 0.0013 | 0.0017 | 0.0008 | 0.0006 |
Cell Periphery | 0.0015 | 0.001 | 0.0009 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.0009 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0006 | 0.0003 | 0.0008 | 0.0011 | 0.0004 |
Cytoplasm | 0.1565 | 0.3312 | 0.3259 | 0.1577 | 0.3109 | 0.3661 | 0.1086 | 0.1592 | 0.133 | 0.1502 | 0.0907 | 0.1785 | 0.0621 | 0.1879 | 0.0907 | 0.1323 | 0.1372 | 0.1434 |
Cytoplasmic Foci | 0.0386 | 0.0505 | 0.0568 | 0.0431 | 0.0608 | 0.0448 | 0.0718 | 0.0627 | 0.0787 | 0.0617 | 0.0491 | 0.0622 | 0.0669 | 0.0569 | 0.0856 | 0.1093 | 0.0618 | 0.0947 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0208 | 0.0142 | 0.0141 | 0.0075 | 0.0118 | 0.0168 | 0.0062 | 0.0074 | 0.0072 | 0.0028 | 0.0033 | 0.0036 | 0.0069 | 0.0095 | 0.0071 | 0.0053 | 0.0044 | 0.0052 |
Endosome | 0.3055 | 0.2088 | 0.1964 | 0.2885 | 0.2613 | 0.2667 | 0.4435 | 0.3417 | 0.3609 | 0.3611 | 0.5017 | 0.408 | 0.3942 | 0.2439 | 0.4025 | 0.3582 | 0.357 | 0.3408 |
Golgi | 0.0036 | 0.0053 | 0.0034 | 0.0024 | 0.0059 | 0.0043 | 0.0041 | 0.0054 | 0.0059 | 0.0031 | 0.006 | 0.004 | 0.0084 | 0.005 | 0.0125 | 0.0048 | 0.0051 | 0.0096 |
Lipid Particles | 0.0134 | 0.011 | 0.0263 | 0.0138 | 0.0129 | 0.0059 | 0.0104 | 0.0064 | 0.0105 | 0.0023 | 0.0066 | 0.0018 | 0.0155 | 0.0091 | 0.0111 | 0.0181 | 0.0083 | 0.0088 |
Mitochondria | 0.0079 | 0.0018 | 0.0028 | 0.0025 | 0.0093 | 0.0117 | 0.0041 | 0.0061 | 0.0114 | 0.0047 | 0.0066 | 0.0048 | 0.0112 | 0.0145 | 0.0253 | 0.0052 | 0.0047 | 0.0193 |
None | 0.1444 | 0.1685 | 0.1746 | 0.2265 | 0.1667 | 0.0889 | 0.0642 | 0.1001 | 0.1129 | 0.0329 | 0.0525 | 0.0293 | 0.1282 | 0.3371 | 0.2311 | 0.1856 | 0.1703 | 0.2079 |
Nuclear Periphery | 0.0118 | 0.0119 | 0.0076 | 0.0072 | 0.007 | 0.0094 | 0.0168 | 0.0116 | 0.0122 | 0.0048 | 0.0088 | 0.0057 | 0.0452 | 0.0076 | 0.0052 | 0.0034 | 0.0035 | 0.005 |
Nucleolus | 0.0019 | 0.0007 | 0.0016 | 0.0005 | 0.0004 | 0.0005 | 0.0012 | 0.0028 | 0.0038 | 0.0006 | 0.0012 | 0.0018 | 0.0034 | 0.0009 | 0.0013 | 0.0021 | 0.0014 | 0.0007 |
Nucleus | 0.0049 | 0.0042 | 0.0057 | 0.0026 | 0.0025 | 0.0041 | 0.0082 | 0.0081 | 0.0106 | 0.0042 | 0.0044 | 0.0134 | 0.0079 | 0.0038 | 0.0024 | 0.0042 | 0.0031 | 0.0024 |
Peroxisomes | 0.0005 | 0.0007 | 0.0018 | 0.0007 | 0.0008 | 0.0007 | 0.0025 | 0.0013 | 0.002 | 0.0008 | 0.0016 | 0.0004 | 0.0052 | 0.0006 | 0.0049 | 0.0029 | 0.0009 | 0.0052 |
Punctate Nuclear | 0.0014 | 0.0011 | 0.0038 | 0.0006 | 0.0006 | 0.0011 | 0.0021 | 0.0025 | 0.006 | 0.0009 | 0.0022 | 0.0015 | 0.0219 | 0.0008 | 0.0012 | 0.0039 | 0.0003 | 0.0028 |
Vacuole | 0.2478 | 0.1603 | 0.1392 | 0.2002 | 0.1294 | 0.1378 | 0.2072 | 0.2083 | 0.1893 | 0.2793 | 0.1914 | 0.2238 | 0.1767 | 0.0943 | 0.09 | 0.1379 | 0.2064 | 0.1161 |
Vacuole Periphery | 0.0362 | 0.0212 | 0.0213 | 0.0451 | 0.0171 | 0.0369 | 0.04 | 0.0496 | 0.038 | 0.0882 | 0.0599 | 0.0536 | 0.0227 | 0.0225 | 0.0229 | 0.0207 | 0.023 | 0.0252 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.5777 | 9.5551 | 11.0899 | 13.1609 | 7.8176 | 8.2175 | 13.0673 | 14.4624 | 13.8766 | 11.3362 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
207 | 1537 | 994 | 942 | 1757 | 1798 | 467 | 1144 | 1964 | 3335 | 1461 | 2086 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 638.20 | 695.43 | 872.41 | 791.16 | 620.89 | 707.10 | 844.71 | 753.56 | 622.71 | 701.72 | 863.56 | 770.54 |
Standard Deviation | 64.55 | 98.85 | 112.56 | 112.39 | 93.00 | 89.75 | 100.67 | 146.33 | 90.58 | 94.23 | 109.66 | 133.40 |
Intensity Change Log 2 | — | 0.123897 | 0.450998 | 0.309961 | — | 0.187577 | 0.444118 | 0.279385 | — | 0.155650 | 0.447609 | 0.294964 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000059 | 0.000201 | 0.000290 | 0.001006 | 0.000074 | 0.000176 | 0.000186 | 0.000534 | 0.000072 | 0.000188 | 0.000257 | 0.000747 |
Bud Neck | 0.038124 | 0.019881 | 0.048360 | 0.017206 | 0.020398 | 0.031270 | 0.009261 | 0.020213 | 0.022266 | 0.026021 | 0.035862 | 0.018855 |
Bud Site | 0.004896 | 0.010683 | 0.020542 | 0.057186 | 0.005883 | 0.016419 | 0.007189 | 0.035023 | 0.005779 | 0.013775 | 0.016274 | 0.045031 |
Cell Periphery | 0.000417 | 0.000094 | 0.000176 | 0.000120 | 0.000118 | 0.000113 | 0.000126 | 0.000342 | 0.000149 | 0.000104 | 0.000160 | 0.000242 |
Cytoplasm | 0.222272* | 0.162102* | 0.027999 | 0.382467* | 0.257475* | 0.103140* | 0.227910* | 0.420299* | 0.253765* | 0.130314* | 0.091900 | 0.403215* |
Cytoplasmic Foci | 0.211291* | 0.256266* | 0.025559 | 0.050414 | 0.227248* | 0.239642* | 0.051072 | 0.064457 | 0.225567* | 0.247303* | 0.033714 | 0.058116 |
Eisosomes | 0.000270 | 0.000073 | 0.000114 | 0.000047 | 0.000102 | 0.000080 | 0.000103 | 0.000145 | 0.000119 | 0.000077 | 0.000110 | 0.000101 |
Endoplasmic Reticulum | 0.005750 | 0.002499 | 0.007038 | 0.002004 | 0.007540 | 0.001980 | 0.003993 | 0.002030 | 0.007352 | 0.002219 | 0.006065 | 0.002019 |
Endosome | 0.117456 | 0.200792* | 0.147074 | 0.020717 | 0.102680 | 0.289736* | 0.031913 | 0.027170 | 0.104238 | 0.248744* | 0.110264 | 0.024256 |
Golgi | 0.002857 | 0.011697 | 0.001867 | 0.002274 | 0.005163 | 0.021409 | 0.000255 | 0.002296 | 0.004920 | 0.016933 | 0.001352 | 0.002286 |
Lipid Particles | 0.049342 | 0.019483 | 0.010574 | 0.003394 | 0.025722 | 0.026909 | 0.009129 | 0.005772 | 0.028212 | 0.023487 | 0.010112 | 0.004698 |
Mitochondria | 0.016956 | 0.020776 | 0.004454 | 0.004491 | 0.015482 | 0.033994 | 0.000635 | 0.001978 | 0.015637 | 0.027902 | 0.003233 | 0.003113 |
Mitotic Spindle | 0.000152 | 0.013127 | 0.021756 | 0.039840 | 0.002148 | 0.019980 | 0.003727 | 0.016657 | 0.001937 | 0.016821 | 0.015993 | 0.027126 |
None | 0.001165 | 0.001210 | 0.001637 | 0.004354 | 0.002375 | 0.001719 | 0.004371 | 0.026496 | 0.002248 | 0.001484 | 0.002511 | 0.016497 |
Nuclear Periphery | 0.001993 | 0.000924 | 0.008108 | 0.002365 | 0.001634 | 0.000991 | 0.007145 | 0.001911 | 0.001672 | 0.000960 | 0.007800 | 0.002116 |
Nuclear Periphery Foci | 0.000533 | 0.001183 | 0.010976 | 0.004158 | 0.000644 | 0.002148 | 0.004358 | 0.004487 | 0.000632 | 0.001703 | 0.008861 | 0.004338 |
Nucleolus | 0.004662 | 0.004713 | 0.065465 | 0.002770 | 0.002424 | 0.006233 | 0.012044 | 0.005366 | 0.002660 | 0.005533 | 0.048389 | 0.004194 |
Nucleus | 0.064214 | 0.020395 | 0.282572* | 0.101490 | 0.056614 | 0.013427 | 0.385401* | 0.124985 | 0.057415 | 0.016638 | 0.315441* | 0.114375 |
Peroxisomes | 0.007655 | 0.011821 | 0.013096 | 0.007818 | 0.004321 | 0.016737 | 0.005188 | 0.004770 | 0.004673 | 0.014472 | 0.010568 | 0.006146 |
Vacuole | 0.231336* | 0.219209* | 0.280761* | 0.288866* | 0.243807* | 0.154277 | 0.234670* | 0.233247* | 0.242492* | 0.184202 | 0.266028* | 0.258363* |
Vacuole Periphery | 0.018599 | 0.022871 | 0.021579 | 0.007013 | 0.018148 | 0.019618 | 0.001324 | 0.001822 | 0.018196 | 0.021117 | 0.015105 | 0.004166 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.70 | -7.80 | -3.54 | -2.35 | -1.63 | -3.65 | -3.68 | -5.38 | -3.27 | -2.39 | -2.83 | -9.05 | -5.37 | -3.45 | -2.17 |
Bud Neck | 2.67 | -1.50 | 3.64 | 3.63 | 10.86 | -4.89 | 4.86 | 2.02 | 6.00 | -2.80 | -2.11 | -5.40 | 4.35 | 6.96 | 8.62 |
Bud Site | -3.91 | -5.88 | -6.52 | -5.11 | -1.76 | -7.16 | -0.46 | -5.83 | -1.50 | -3.69 | -8.29 | -5.57 | -8.73 | -4.73 | -2.70 |
Cell Periphery | 3.94 | 3.02 | 4.03 | 0.28 | 4.72 | 0.29 | -0.33 | -1.28 | -1.33 | -1.22 | 3.09 | -0.22 | 0.17 | -1.25 | 0.27 |
Cytoplasm | 3.66 | 12.49 | -5.60 | -15.18 | -31.69 | 22.08 | 2.81 | -10.82 | -28.83 | -10.66 | 19.43 | 23.28 | -11.33 | -31.13 | -33.93 |
Cytoplasmic Foci | -3.64 | 15.98 | 15.02 | 42.30 | -3.78 | -2.42 | 34.44 | 36.41 | 37.24 | -0.73 | -4.58 | 45.75 | 42.44 | 55.69 | -4.93 |
Eisosomes | 4.92 | 3.83 | 5.46 | 5.74 | 11.64 | 4.35 | -0.88 | 0.37 | -0.52 | 0.63 | 7.17 | 0.85 | 3.30 | 0.24 | 2.98 |
Endoplasmic Reticulum | 2.22 | -1.16 | 2.18 | -0.28 | 12.10 | 7.78 | 4.07 | 7.35 | -1.81 | 5.58 | 7.89 | 1.19 | 7.60 | -1.49 | 13.52 |
Endosome | -7.13 | -1.72 | 10.21 | 35.31* | 19.40 | -27.56* | 14.40 | 22.68 | 44.07* | 2.39 | -27.97 | 0.13 | 26.79 | 55.44* | 18.06 |
Golgi | -8.20 | 1.58 | 2.84 | 11.96 | 3.03 | -13.16 | 13.18 | 6.15 | 15.66 | -1.56 | -14.52 | 10.23 | 8.36 | 19.43 | 0.94 |
Lipid Particles | 4.66 | 6.15 | 7.41 | 12.19 | 8.11 | -0.83 | 8.91 | 15.48 | 13.02 | 2.48 | 2.73 | 12.52 | 18.73 | 17.51 | 6.88 |
Mitochondria | -0.63 | 2.33 | 2.74 | 11.55 | 3.28 | -7.33 | 11.09 | 10.28 | 15.24 | -1.88 | -6.41 | 9.27 | 10.26 | 19.02 | 3.70 |
Mitotic Spindle | -7.52 | -8.02 | -7.37 | -3.21 | 0.46 | -8.94 | -1.90 | -6.99 | 0.43 | -4.56 | -10.65 | -7.60 | -9.61 | -2.11 | -0.26 |
None | 0.05 | -0.45 | -3.02 | -4.86 | -3.64 | 0.71 | -1.77 | -4.70 | -4.96 | -3.81 | 1.21 | -0.26 | -4.89 | -5.65 | -4.95 |
Nuclear Periphery | 3.10 | -8.21 | -2.52 | -8.47 | 7.38 | 2.67 | -4.19 | -5.91 | -8.77 | 2.82 | 3.58 | -9.59 | -6.54 | -12.08 | 7.41 |
Nuclear Periphery Foci | -2.48 | -7.79 | -4.12 | -2.72 | 5.94 | -4.85 | -4.16 | -6.42 | -2.84 | 1.38 | -5.26 | -8.70 | -7.47 | -3.94 | 5.83 |
Nucleolus | 0.08 | -11.07 | 0.96 | 3.72 | 15.21 | -5.07 | -4.30 | -2.66 | 1.96 | 3.43 | -4.73 | -15.20 | -1.12 | 3.57 | 14.94 |
Nucleus | 6.29 | -17.21 | -8.86 | -20.33 | 11.15 | 17.06 | -23.13 | -14.28 | -24.16 | 16.60 | 17.24 | -29.09 | -18.51 | -31.44 | 18.44 |
Peroxisomes | -2.97 | -3.25 | 2.41 | 6.23 | 5.83 | -12.57 | -0.64 | 1.55 | 12.43 | 1.24 | -13.84 | -5.80 | 1.75 | 13.20 | 6.32 |
Vacuole | 0.77 | -2.75 | -8.88 | -15.07 | -8.62 | 13.14 | -0.50 | -9.05 | -19.00 | -5.80 | 9.76 | -3.08 | -14.27 | -23.87 | -9.61 |
Vacuole Periphery | -1.22 | -0.31 | 5.18 | 14.66 | 10.34 | -0.97 | 15.98 | 15.67 | 21.10 | 0.46 | -2.21 | 3.12 | 15.43 | 24.50 | 11.05 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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