Standard name | |
---|---|
Human Ortholog | |
Description | Peroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 1 | 0 | 0 | 1 | 5 | 4 | 4 | – | 3 | 2 | 0 | 11 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 14 | 16 |
Bud | – | 2 | 2 | 8 | 23 | 40 | 63 | 57 | – | 54 | 45 | 58 | 13 | 13 | 15 | 6 | 4 | 5 | 1 | 6 | 11 | 7 | 27 | 35 |
Bud Neck | – | 2 | 10 | 16 | 25 | 20 | 31 | 17 | – | 11 | 7 | 11 | 25 | 19 | 24 | 14 | 20 | 5 | 2 | 7 | 12 | 8 | 22 | 37 |
Bud Site | – | 1 | 25 | 29 | 41 | 53 | 48 | 30 | – | 55 | 36 | 32 | 27 | 28 | 24 | 10 | 11 | 8 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 0 | 1 | 3 | 8 | 5 | 5 | 10 | – | 6 | 3 | 8 | 4 | 3 | 10 | 13 | 8 | 8 | 1 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | – | 2 | 4 | 8 | 8 | 0 | 12 | 8 | – | 12 | 4 | 15 | 5 | 12 | 28 | 13 | 22 | 7 | 0 | 0 | 2 | 9 | 19 | 25 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 |
Mitochondria | – | 4 | 26 | 36 | 66 | 80 | 106 | 83 | – | 92 | 72 | 110 | 36 | 25 | 13 | 25 | 18 | 11 | 0 | 0 | 2 | 1 | 10 | 15 |
Nucleus | – | 0 | 0 | 2 | 7 | 4 | 11 | 3 | – | 2 | 2 | 5 | 4 | 3 | 4 | 6 | 7 | 6 | 0 | 0 | 0 | 3 | 2 | 2 |
Nuclear Periphery | – | 0 | 0 | 1 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | – | 7 | 27 | 43 | 80 | 88 | 139 | 118 | – | 93 | 56 | 99 | 67 | 74 | 72 | 82 | 98 | 79 | 1 | 7 | 10 | 12 | 24 | 26 |
Peroxisomes | – | 10 | 36 | 136 | 188 | 139 | 246 | 175 | – | 276 | 132 | 225 | 90 | 177 | 237 | 44 | 54 | 37 | 2 | 8 | 21 | 41 | 137 | 204 |
SpindlePole | – | 5 | 52 | 122 | 179 | 143 | 226 | 212 | – | 231 | 125 | 179 | 166 | 214 | 307 | 110 | 118 | 97 | 11 | 16 | 49 | 44 | 114 | 100 |
Vac/Vac Membrane | – | 1 | 1 | 1 | 0 | 1 | 0 | 6 | – | 4 | 4 | 3 | 0 | 2 | 3 | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 6 | 4 |
Unique Cell Count | 25 | 113 | 254 | 422 | 358 | 562 | 482 | 514 | 308 | 477 | 310 | 394 | 496 | 219 | 241 | 175 | 24 | 55 | 118 | 140 | 396 | 483 | ||
Labelled Cell Count | 35 | 184 | 406 | 627 | 578 | 892 | 725 | 840 | 488 | 746 | 448 | 575 | 739 | 328 | 367 | 264 | 24 | 55 | 118 | 140 | 396 | 483 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 10.5 | 8.4 | 9.8 | 9.2 | 8.8 | 8.8 | 9.2 | – | 10.1 | 9.3 | 9.8 | 10.8 | 10.9 | 10.5 | 15.1 | 15.6 | 14.9 | 10.5 | 10.6 | 10.8 |
Std Deviation (1e-4) | – | 3.9 | 4.8 | 6.2 | 4.9 | 5.4 | 5.7 | 5.8 | – | 7.4 | 5.2 | 6.3 | 4.5 | 5.0 | 4.3 | 12.1 | 13.2 | 12.0 | 4.7 | 5.7 | 5.0 |
Intensity Change (Log2) | – | – | – | 0.22 | 0.13 | 0.06 | 0.06 | 0.12 | – | 0.26 | 0.13 | 0.22 | 0.35 | 0.37 | 0.32 | 0.84 | 0.89 | 0.82 | 0.31 | 0.33 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1.6 | 3.1 | 3.1 | 3.2 | – | 2.9 | 3.7 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -0.9 | -1.1 | -1.2 | -1.4 | -2.4 | – | 0 | 0 | 0 | -0.3 | -1.6 | -1.7 | -0.8 | -0.2 | -2.2 |
Bud Site | -2.7 | -3.6 | -1.8 | -4.2 | -5.3 | – | -3.3 | -2.7 | -5.0 | -3.7 | -4.6 | -6.1 | -4.9 | -5.1 | -4.6 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.9 | 0.9 | 1.9 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.1 | -1.8 | -0.1 | -1.0 | -1.4 | – | -1.3 | 0.1 | 0 | -2.9 | -5.2 | -8.0 | -2.8 | -4.1 | -4.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.6 | -1.2 | 0.1 | 0.2 | 0.1 | – | -1.4 | -1.3 | -0.7 | -0.5 | -1.2 | -2.4 | 2.5 | 3.1 | 3.7 |
Peroxisomes | 3.8 | 2.4 | 1.3 | 2.3 | 0.9 | – | 4.2 | 2.0 | 2.9 | -0.6 | 2.5 | 3.1 | -2.4 | -1.9 | -2.0 |
SpindlePole | 0.4 | -0.7 | -1.1 | -1.1 | -0.4 | – | -0.2 | -1.0 | -1.7 | 1.4 | 1.6 | 3.1 | 0.7 | 0.5 | 1.6 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.488 | 11.6846 | 11.4685 | 11.0743 | 11.6643 | 11.2928 | 10.3688 | 10.3283 | 11.1185 | 10.0807 | 9.0966 | 9.5428 | 8.7317 | 12.8838 | 10.7963 | 10.4845 | 9.1358 | 11.078 |
Actin | 0.0176 | 0.0108 | 0.0312 | 0.0025 | 0.0071 | 0.0041 | 0.0185 | 0.0016 | 0.0112 | 0.0003 | 0.0133 | 0.0007 | 0.0174 | 0.0061 | 0.0282 | 0.0015 | 0.0299 | 0.01 |
Bud | 0.0009 | 0.0097 | 0.005 | 0.0009 | 0.0002 | 0.0004 | 0.0004 | 0.0049 | 0.0044 | 0.0003 | 0.0001 | 0.0004 | 0.0006 | 0.0192 | 0.0067 | 0.0002 | 0.0028 | 0.0003 |
Bud Neck | 0.0034 | 0.0092 | 0.0341 | 0.0241 | 0.0007 | 0.0526 | 0.0034 | 0.005 | 0.0454 | 0.056 | 0.0139 | 0.0066 | 0.0012 | 0.003 | 0.027 | 0.0486 | 0.0051 | 0.0147 |
Bud Periphery | 0.001 | 0.0058 | 0.0032 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0013 | 0.0022 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0.0067 | 0.0026 | 0.0001 | 0.0026 | 0.0002 |
Bud Site | 0.0094 | 0.0777 | 0.0359 | 0.0036 | 0.0006 | 0.0017 | 0.0059 | 0.0682 | 0.0219 | 0.0043 | 0.0005 | 0.0016 | 0.0102 | 0.0409 | 0.0259 | 0.0007 | 0.0033 | 0.0013 |
Cell Periphery | 0.0007 | 0.0032 | 0.0012 | 0.0007 | 0.0004 | 0.0006 | 0.0006 | 0.0018 | 0.0015 | 0.0007 | 0.0003 | 0.0003 | 0.001 | 0.0017 | 0.0017 | 0.0006 | 0.0009 | 0.0005 |
Cytoplasm | 0.0013 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.0008 | 0.0003 | 0 | 0.0026 | 0 | 0.0009 | 0.0006 | 0.0002 | 0.0006 | 0.0008 | 0.0001 |
Cytoplasmic Foci | 0.2301 | 0.277 | 0.253 | 0.2589 | 0.1416 | 0.2406 | 0.2048 | 0.2904 | 0.2367 | 0.2221 | 0.2861 | 0.2942 | 0.2101 | 0.2451 | 0.231 | 0.2771 | 0.2286 | 0.2813 |
Eisosomes | 0.0008 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0061 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0085 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0003 | 0 |
Endosome | 0.0291 | 0.004 | 0.004 | 0.0037 | 0.0109 | 0.0009 | 0.0072 | 0.0018 | 0.004 | 0.0005 | 0.018 | 0.0008 | 0.0073 | 0.0073 | 0.0019 | 0.0187 | 0.021 | 0.001 |
Golgi | 0.0125 | 0.0047 | 0.0076 | 0.0086 | 0.0365 | 0.0046 | 0.0049 | 0.0035 | 0.0033 | 0.0027 | 0.0115 | 0.0035 | 0.0055 | 0.0115 | 0.0039 | 0.0041 | 0.0276 | 0.0068 |
Lipid Particles | 0.1457 | 0.0852 | 0.1082 | 0.1791 | 0.1988 | 0.1793 | 0.1329 | 0.0992 | 0.0891 | 0.2711 | 0.1693 | 0.2026 | 0.1999 | 0.1184 | 0.1043 | 0.1711 | 0.2093 | 0.1978 |
Mitochondria | 0.1254 | 0.0753 | 0.0751 | 0.0808 | 0.1057 | 0.0644 | 0.1602 | 0.0853 | 0.1242 | 0.059 | 0.1056 | 0.0554 | 0.1136 | 0.0917 | 0.1439 | 0.1303 | 0.0604 | 0.1134 |
None | 0.0016 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0073 | 0.0021 | 0.0004 | 0 | 0.0036 | 0.0001 | 0.0082 | 0.0004 | 0.0009 | 0.0011 | 0.0003 | 0 |
Nuclear Periphery | 0.0028 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0017 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0.0023 | 0.0002 | 0.0001 | 0.0022 | 0.0002 | 0 |
Nucleolus | 0.0588 | 0.089 | 0.0881 | 0.0941 | 0.0587 | 0.038 | 0.0702 | 0.1189 | 0.0542 | 0.0288 | 0.0348 | 0.0412 | 0.0673 | 0.0982 | 0.0621 | 0.0555 | 0.1534 | 0.0214 |
Nucleus | 0.0017 | 0.0051 | 0.0156 | 0.003 | 0.0014 | 0.0009 | 0.0019 | 0.0035 | 0.0028 | 0.0009 | 0.0009 | 0.0008 | 0.0033 | 0.0172 | 0.0029 | 0.0018 | 0.0083 | 0.0006 |
Peroxisomes | 0.3446 | 0.3116 | 0.3321 | 0.3056 | 0.4342 | 0.4093 | 0.3579 | 0.2985 | 0.3846 | 0.3511 | 0.3073 | 0.3876 | 0.3289 | 0.2976 | 0.3459 | 0.2796 | 0.2391 | 0.3483 |
Punctate Nuclear | 0.0017 | 0.0146 | 0.0026 | 0.0322 | 0.0005 | 0.0009 | 0.0173 | 0.0038 | 0.0093 | 0.0009 | 0.021 | 0.003 | 0.0065 | 0.0032 | 0.008 | 0.0019 | 0.0024 | 0.0008 |
Vacuole | 0.0028 | 0.011 | 0.0011 | 0.0006 | 0.0012 | 0.0006 | 0.0009 | 0.0054 | 0.003 | 0.0006 | 0.0006 | 0.0005 | 0.0086 | 0.0176 | 0.0016 | 0.0017 | 0.0026 | 0.0007 |
Vacuole Periphery | 0.002 | 0.0052 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0006 | 0.0036 | 0.001 | 0.0003 | 0.0004 | 0.0003 | 0.0054 | 0.0131 | 0.0005 | 0.0009 | 0.001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 96.9941 | 51.3304 | 32.9422 | 36.3202 | 50.3028 | 80.6544 | 63.1218 | 43.5497 | 41.5117 | 64.8505 |
Translational Efficiency | 1.3589 | 1.5771 | 1.5294 | 1.323 | 1.5855 | 2.1867 | 1.3355 | 1.4386 | 1.8424 | 1.3938 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
106 | 1260 | 714 | 922 | 1630 | 1433 | 2439 | 1716 | 1736 | 2693 | 3153 | 2638 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 877.81 | 1104.59 | 1163.74 | 1434.36 | 909.70 | 1104.00 | 1316.98 | 1499.11 | 907.75 | 1104.28 | 1282.28 | 1476.48 |
Standard Deviation | 180.48 | 362.24 | 289.88 | 432.56 | 307.56 | 337.40 | 437.84 | 459.85 | 301.44 | 349.24 | 414.05 | 451.55 |
Intensity Change Log 2 | — | 0.331530 | 0.406788 | 0.708427 | — | 0.279277 | 0.533771 | 0.720643 | — | 0.305174 | 0.472808 | 0.714657 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004309 | 0.006544 | 0.015445 | 0.017555 | 0.005596 | 0.007225 | 0.008067 | 0.028926 | 0.005518 | 0.006906 | 0.009738 | 0.024952 |
Bud Neck | 0.015091 | 0.015519 | 0.019621 | 0.022912 | 0.015980 | 0.022575 | 0.012758 | 0.015681 | 0.015926 | 0.019274 | 0.014312 | 0.018208 |
Bud Site | 0.094950 | 0.062772 | 0.070275 | 0.107767* | 0.050867 | 0.073928 | 0.058361 | 0.082673 | 0.053559 | 0.068708 | 0.061059 | 0.091443 |
Cell Periphery | 0.001160 | 0.000399 | 0.000222 | 0.000336 | 0.000482 | 0.000494 | 0.000296 | 0.000382 | 0.000524 | 0.000449 | 0.000279 | 0.000366 |
Cytoplasm | 0.016843 | 0.012825 | 0.011317 | 0.017530 | 0.014372 | 0.013912 | 0.017885 | 0.020453 | 0.014523 | 0.013403 | 0.016398 | 0.019431 |
Cytoplasmic Foci | 0.298426* | 0.326653* | 0.300169* | 0.307981* | 0.271504* | 0.328444* | 0.236612* | 0.233074* | 0.273147* | 0.327606* | 0.251005* | 0.259255* |
Eisosomes | 0.000624 | 0.002558 | 0.001270 | 0.001556 | 0.002462 | 0.001987 | 0.002636 | 0.001725 | 0.002349 | 0.002254 | 0.002327 | 0.001666 |
Endoplasmic Reticulum | 0.000727 | 0.000329 | 0.000329 | 0.000198 | 0.000381 | 0.000421 | 0.000156 | 0.000207 | 0.000402 | 0.000378 | 0.000195 | 0.000204 |
Endosome | 0.019502 | 0.004297 | 0.006578 | 0.006789 | 0.005504 | 0.007162 | 0.001614 | 0.004212 | 0.006358 | 0.005822 | 0.002738 | 0.005113 |
Golgi | 0.020167 | 0.008593 | 0.010224 | 0.011215 | 0.013755 | 0.009646 | 0.002829 | 0.011737 | 0.014147 | 0.009153 | 0.004504 | 0.011554 |
Lipid Particles | 0.008203 | 0.008517 | 0.008460 | 0.007042 | 0.009610 | 0.010677 | 0.003073 | 0.007007 | 0.009524 | 0.009667 | 0.004293 | 0.007019 |
Mitochondria | 0.013370 | 0.020795 | 0.016148 | 0.049201 | 0.019430 | 0.034759 | 0.048813 | 0.057847 | 0.019060 | 0.028226 | 0.041416 | 0.054825 |
Mitotic Spindle | 0.012687 | 0.009382 | 0.050564 | 0.035294 | 0.006528 | 0.018752 | 0.018848 | 0.022136 | 0.006904 | 0.014368 | 0.026030 | 0.026735 |
None | 0.003105 | 0.000546 | 0.000191 | 0.000351 | 0.001445 | 0.000595 | 0.000443 | 0.000402 | 0.001546 | 0.000572 | 0.000386 | 0.000384 |
Nuclear Periphery | 0.000051 | 0.000069 | 0.000241 | 0.000193 | 0.000057 | 0.000155 | 0.000115 | 0.000137 | 0.000056 | 0.000115 | 0.000143 | 0.000156 |
Nuclear Periphery Foci | 0.002018 | 0.001066 | 0.001352 | 0.000521 | 0.001860 | 0.001741 | 0.000314 | 0.000264 | 0.001870 | 0.001425 | 0.000549 | 0.000354 |
Nucleolus | 0.006452 | 0.006918 | 0.003867 | 0.003496 | 0.005019 | 0.006182 | 0.003649 | 0.002725 | 0.005107 | 0.006526 | 0.003698 | 0.002995 |
Nucleus | 0.002011 | 0.003055 | 0.001488 | 0.001862 | 0.003568 | 0.002705 | 0.001451 | 0.001954 | 0.003473 | 0.002869 | 0.001460 | 0.001922 |
Peroxisomes | 0.476733* | 0.504989* | 0.477968* | 0.400241* | 0.567489* | 0.452862* | 0.579792* | 0.504861* | 0.561947* | 0.477251* | 0.556734* | 0.468295* |
Vacuole | 0.003273 | 0.003765 | 0.003781 | 0.006782 | 0.003816 | 0.005090 | 0.001954 | 0.002994 | 0.003783 | 0.004470 | 0.002368 | 0.004318 |
Vacuole Periphery | 0.000297 | 0.000409 | 0.000491 | 0.001179 | 0.000275 | 0.000689 | 0.000333 | 0.000602 | 0.000277 | 0.000558 | 0.000368 | 0.000804 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.64 | -4.10 | -5.32 | -4.52 | -0.60 | -1.22 | -2.51 | -9.40 | -8.17 | -8.24 | -1.45 | -4.19 | -10.52 | -9.48 | -7.84 |
Bud Neck | -0.18 | -1.59 | -2.34 | -3.73 | -1.27 | -3.91 | 2.33 | -0.04 | 4.45 | -3.10 | -2.49 | 1.08 | -1.90 | 0.74 | -3.98 |
Bud Site | 1.87 | 1.36 | -0.81 | -6.70 | -4.97 | -4.98 | -2.11 | -7.12 | -1.95 | -5.53 | -3.93 | -2.23 | -9.21 | -5.91 | -7.93 |
Cell Periphery | 1.06 | 1.27 | 1.10 | 0.51 | -2.58 | -0.13 | 3.01 | 0.94 | 0.91 | -1.26 | 0.95 | 3.39 | 1.69 | 0.91 | -1.80 |
Cytoplasm | 1.07 | 1.31 | -0.11 | -3.64 | -4.08 | 0.53 | -2.98 | -4.26 | -4.72 | -1.94 | 1.37 | -1.77 | -4.24 | -6.08 | -2.91 |
Cytoplasmic Foci | -0.83 | 0.02 | -0.36 | 1.13 | -0.81 | -5.49 | 3.83 | 3.81 | 9.30 | 0.26 | -6.18 | 2.54 | 1.23 | 8.36 | -1.46 |
Eisosomes | -7.96 | -4.64 | -5.21 | 3.76 | -1.40 | 2.27 | -0.65 | 4.08 | 1.46 | 4.11 | 0.53 | 0.17 | 4.24 | 3.78 | 3.80 |
Endoplasmic Reticulum | 1.24 | 1.20 | 1.69 | 2.18 | 2.29 | -0.31 | 5.15 | 3.95 | 2.32 | -1.42 | 0.48 | 4.49 | 4.60 | 3.04 | -0.11 |
Endosome | 2.15 | 1.81 | 1.78 | -1.83 | -0.09 | -1.44 | 5.91 | 1.66 | 2.73 | -4.52 | 0.58 | 4.68 | 1.43 | 0.89 | -3.96 |
Golgi | 2.55 | 2.18 | 1.92 | -1.26 | -0.42 | 2.89 | 10.23 | 1.31 | -1.22 | -6.69 | 4.11 | 9.09 | 1.93 | -1.83 | -6.48 |
Lipid Particles | 0.05 | 0.08 | 0.45 | 0.76 | 0.86 | -0.47 | 4.93 | 1.79 | 2.03 | -4.01 | -0.01 | 4.15 | 1.90 | 2.02 | -3.19 |
Mitochondria | -1.32 | -0.50 | -5.10 | -5.68 | -6.80 | -4.45 | -9.32 | -10.38 | -5.26 | -2.09 | -3.62 | -8.33 | -11.43 | -8.20 | -3.87 |
Mitotic Spindle | 0.62 | -4.69 | -3.21 | -7.16 | 2.41 | -5.73 | -8.22 | -7.89 | -1.12 | -1.56 | -5.16 | -10.82 | -10.54 | -5.93 | -0.38 |
None | 1.07 | 1.21 | 1.15 | 2.00 | -3.49 | 2.38 | 3.00 | 3.10 | 1.30 | 0.61 | 2.77 | 3.36 | 3.35 | 2.03 | -0.06 |
Nuclear Periphery | -1.01 | -5.39 | -6.10 | -6.68 | 1.44 | -5.32 | -2.91 | -8.05 | 0.76 | -0.95 | -5.45 | -5.08 | -10.59 | -3.40 | -0.56 |
Nuclear Periphery Foci | 1.47 | 0.82 | 2.33 | 3.25 | 1.99 | 0.65 | 5.20 | 5.36 | 5.34 | 1.02 | 1.71 | 4.50 | 5.36 | 6.49 | 1.88 |
Nucleolus | -0.17 | 0.55 | 0.70 | 2.82 | 0.47 | -1.37 | 1.90 | 3.04 | 4.26 | 1.44 | -1.80 | 1.85 | 2.85 | 5.11 | 1.28 |
Nucleus | -1.28 | 0.25 | -0.29 | 1.01 | -0.60 | 1.03 | 2.71 | 1.17 | 0.36 | -1.56 | 0.82 | 2.68 | 1.33 | 0.89 | -1.73 |
Peroxisomes | -0.92 | 0.03 | 2.26 | 7.69 | 4.85 | 9.44 | -0.98 | 5.74 | -3.99 | 7.33 | 8.11 | 0.77 | 9.44 | 1.52 | 10.25 |
Vacuole | -0.48 | -0.99 | -2.51 | -2.48 | -1.82 | -1.75 | 4.01 | 1.19 | 2.75 | -3.04 | -1.23 | 2.75 | -1.19 | -0.05 | -3.76 |
Vacuole Periphery | -0.75 | -1.20 | -2.49 | -2.28 | -1.74 | -3.23 | -0.63 | -3.45 | 0.50 | -2.33 | -3.17 | -1.18 | -3.90 | -1.76 | -2.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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