Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – – 0.06 0 0.08 0 0 0 0 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0 0 0 0.06 0.05 0 0.09 0.07 0.23 0.06 0 – – 0.06 0.06 0 0 0 0 0 0 0
Nucleus 0.93 0.91 0.98 0.93 0.95 0.9 0.87 0.88 0.82 0.83 0.78 0.82 1.0 – – 0.84 0.86 0.85 0.81 0.84 0.85 0.7 0.54 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0 0 0 0 0.09 0.13 0.15 0.23 0.17 0.34 0.28 0.26 – – 0 0.06 0 0.11 0.07 0.07 0.06 0.06 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0.06 0 0 0 0 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 1 2 2 0 0 1 0 – – 0 0 0 0 0 0 1 1 0
Bud Neck 0 0 0 0 1 0 0 1 2 0 1 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 3 7 5 3 4 5 0 – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Cytoplasm 6 11 3 12 7 9 15 17 8 7 7 9 0 – – 6 2 7 0 0 2 0 1 2
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 1 0 0 0 0 0 0
Endosome 0 1 1 2 2 0 0 0 0 0 1 0 0 – – 1 1 1 2 5 0 1 1 0
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 – – 1 1 0 1 4 0 0 0 0
Mitochondria 11 24 0 5 2 20 21 17 24 19 53 17 0 – – 6 3 1 5 2 0 0 0 1
Nucleus 204 337 215 229 286 302 377 411 221 217 178 235 23 – – 90 44 71 184 322 198 22 11 37
Nuclear Periphery 1 1 1 3 1 1 6 0 1 1 2 1 0 – – 2 0 0 1 0 0 0 0 0
Nucleolus 29 9 2 9 13 31 55 70 63 45 78 80 6 – – 3 3 2 25 28 16 1 1 5
Peroxisomes 0 0 0 0 0 1 1 0 0 0 0 0 0 – – 0 0 0 1 0 0 0 0 0
SpindlePole 0 4 6 2 4 1 3 9 3 5 2 2 0 – – 1 0 0 1 4 1 0 0 0
Vac/Vac Membrane 1 9 1 1 3 3 3 5 1 2 1 1 0 – – 5 1 5 1 13 9 1 1 4
Unique Cell Count 220 369 220 247 302 337 434 469 269 260 229 285 23 107 51 84 226 386 233 33 21 54
Labelled Cell Count 254 399 229 263 319 369 485 539 330 299 327 351 29 115 56 88 226 386 233 33 21 54


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.4 7.6 6.9 7.5 6.4 6.5 6.8 5.8 6.2 5.4 6.0 6.5 – – 8.6 9.3 8.9 8.3 – 9.0
Std Deviation (1e-4) 1.0 1.1 1.6 1.6 1.5 1.3 1.6 1.6 1.3 1.4 1.2 1.2 1.0 – – 1.8 2.2 2.1 1.4 – 2.0
Intensity Change (Log2) – – – -0.14 -0.03 -0.26 -0.24 -0.16 -0.39 -0.29 -0.49 -0.35 -0.24 – – 0.17 0.28 0.23 0.12 – 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cytoplasm 2.1 0 0 1.5 1.6 0 0 0 1.3 0 – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Mitochondria 0 0 3.7 3.3 2.9 4.5 4.1 7.6 3.7 0 – – 0 0 0
Nucleus -2.5 -1.7 -3.6 -4.5 -4.3 -5.5 -5.2 -6.4 -5.5 0.7 – – -4.6 -3.6 -4.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nucleolus 1.9 2.3 4.1 5.0 5.6 7.3 6.0 9.2 8.2 0 – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
SpindlePole 0 0 0 0 0 0 -0.6 0 0 0 – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3462 2.2669 2.3166 2.2099 2.5747 2.1113 1.8325 1.8581 1.7191 1.9127 1.1155 1.5898 3.2256 2.2934 2.3135 2.1375 1.9514 2.1041
Actin 0.0112 0 0.0028 0.006 0.0002 0.0001 0.0014 0.0001 0.0011 0 0.0037 0.0027 0.0184 0 0.0119 0.0105 0.0088 0.0001
Bud 0.0006 0 0.0004 0.0003 0.0013 0.0001 0.0005 0.0001 0.0003 0 0.0003 0.0006 0.0008 0 0.0006 0.0009 0.0024 0.0002
Bud Neck 0.0027 0.0001 0.0004 0.0007 0.0026 0.0008 0.0011 0.0001 0.0002 0 0.0024 0.0028 0.0016 0.0001 0.0005 0.0144 0.0133 0.0019
Bud Periphery 0.0023 0 0.0014 0.0004 0.0034 0.0004 0.0008 0 0.0002 0 0.0007 0.001 0.0014 0 0.0008 0.0037 0.0061 0.0004
Bud Site 0.0019 0 0.0006 0.0062 0.0045 0.0002 0.0045 0.0035 0.0013 0 0.0004 0.0014 0.0029 0.0001 0.0061 0.0022 0.0098 0.0003
Cell Periphery 0.001 0 0.0004 0.0003 0.001 0.0001 0.0002 0 0 0 0.0002 0.0002 0.0003 0 0.0004 0.0023 0.0018 0.0001
Cytoplasm 0.0028 0.0001 0.0005 0.0045 0.0015 0.0002 0.0064 0.0001 0.0163 0 0.0013 0.0016 0.0281 0.0019 0.0032 0.0002 0.0007 0.0003
Cytoplasmic Foci 0.008 0 0.0009 0.005 0.0064 0.0002 0.0234 0.0026 0.0095 0 0.0001 0.0147 0.0413 0.0001 0.0123 0.0008 0.0022 0
Eisosomes 0.0002 0 0.0001 0.0002 0 0 0.0001 0 0 0 0.0001 0 0.001 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0038 0 0.0013 0.0023 0.0006 0.0008 0.0039 0 0.0015 0 0.0068 0.0008 0.0086 0.0005 0.0005 0.0009 0.0008 0
Endosome 0.0229 0 0.0055 0.0075 0.0492 0.0049 0.0272 0.0002 0.0158 0 0.0025 0.0309 0.0307 0.0015 0.0129 0.0142 0.021 0.0001
Golgi 0.0042 0 0.0026 0.0014 0.0043 0.0005 0.0038 0.0003 0.0012 0 0.0003 0.0111 0.0053 0 0.0073 0.0128 0.0327 0
Lipid Particles 0.0093 0 0.0073 0.0096 0.0022 0.001 0.017 0.0004 0.002 0 0.0003 0.0121 0.0218 0.0002 0.0082 0.0037 0.0034 0
Mitochondria 0.0096 0 0.0145 0.008 0.0058 0.0024 0.0024 0.0002 0.0008 0 0.003 0.0069 0.0069 0.0002 0.0177 0.0164 0.0074 0.0009
None 0.0028 0 0.0003 0.0024 0.0002 0.0001 0.0057 0.0001 0.0014 0 0.0002 0.0004 0.062 0.0011 0.0007 0.0001 0.0001 0.0001
Nuclear Periphery 0.0338 0.0011 0.0061 0.0046 0.0015 0.0085 0.0218 0.0005 0.0031 0.0001 0.059 0.0065 0.0837 0.0046 0.0034 0.0011 0.0016 0.0005
Nucleolus 0.0667 0.0428 0.0263 0.0332 0.1424 0.1144 0.0221 0.0237 0.0218 0.0082 0.0687 0.0594 0.0293 0.0303 0.0388 0.0264 0.0673 0.1132
Nucleus 0.7661 0.9524 0.9175 0.8963 0.7474 0.8489 0.8041 0.9658 0.9171 0.9913 0.8442 0.8222 0.576 0.9377 0.8603 0.8732 0.7957 0.8695
Peroxisomes 0.0029 0 0.0024 0.0013 0.0005 0.0001 0.0046 0.0013 0.0011 0 0.0001 0.0181 0.0074 0 0.0043 0.0004 0.0005 0
Punctate Nuclear 0.0338 0.0033 0.0035 0.0059 0.0018 0.0108 0.0437 0.0009 0.004 0.0002 0.0025 0.0035 0.0606 0.0192 0.0031 0.0014 0.0027 0.0121
Vacuole 0.0093 0 0.0031 0.0029 0.02 0.0037 0.0041 0.0001 0.001 0 0.0017 0.0022 0.0056 0.0019 0.0055 0.0104 0.0195 0.0003
Vacuole Periphery 0.004 0 0.002 0.0012 0.0032 0.0018 0.0013 0 0.0003 0 0.0015 0.0009 0.006 0.0005 0.0013 0.0036 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.8944 16.8472 16.534 17.9419 15.3702 13.9748 15.7978 19.0997 15.7776 18.5199
Translational Efficiency 0.5451 0.6187 0.7122 0.686 0.712 0.6108 0.6928 0.6394 0.7563 0.6404

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2185 1627 1270 128 1858 2047 1677 1990 4043 3674 2947 2118

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 701.36 797.77 994.44 1132.05 790.56 780.73 902.48 956.44 742.35 788.28 942.11 967.05
Standard Deviation 82.59 126.29 130.58 144.16 98.33 123.29 150.78 137.43 100.53 124.91 149.53 144.05
Intensity Change Log 2 — 0.185818 0.503729 0.690711 — -0.018051 0.191020 0.274800 — 0.081384 0.346489 0.485290

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000043 0.000462 0.001110 0.001508 0.000156 0.000356 0.000877 0.000985 0.000095 0.000403 0.000978 0.001017
Bud Neck 0.002740 0.009768 0.022002 0.020736 0.004750 0.008390 0.020280 0.045388 0.003664 0.009000 0.021022 0.043898
Bud Site 0.000667 0.001535 0.001586 0.002054 0.000226 0.001089 0.001689 0.005321 0.000465 0.001286 0.001645 0.005124
Cell Periphery 0.001136 0.000093 0.000043 0.000080 0.000026 0.000116 0.000079 0.000138 0.000626 0.000106 0.000063 0.000134
Cytoplasm 0.030244 0.003647 0.000526 0.001308 0.000993 0.003215 0.001484 0.005232 0.016801 0.003406 0.001071 0.004995
Cytoplasmic Foci 0.004645 0.001315 0.000070 0.000031 0.000102 0.001772 0.000036 0.001941 0.002557 0.001570 0.000051 0.001826
Eisosomes 0.000056 0.000028 0.000049 0.000048 0.000016 0.000027 0.000033 0.000037 0.000038 0.000027 0.000040 0.000037
Endoplasmic Reticulum 0.000799 0.003060 0.003838 0.006121 0.001516 0.002826 0.003442 0.005419 0.001128 0.002930 0.003613 0.005462
Endosome 0.000350 0.000595 0.000201 0.000214 0.000083 0.001203 0.000173 0.003244 0.000227 0.000934 0.000185 0.003061
Golgi 0.000117 0.000454 0.000142 0.000120 0.000043 0.000719 0.000109 0.001260 0.000083 0.000602 0.000123 0.001191
Lipid Particles 0.000547 0.000560 0.000141 0.000112 0.000079 0.000593 0.000057 0.000170 0.000332 0.000578 0.000093 0.000166
Mitochondria 0.000677 0.002446 0.001520 0.002019 0.000346 0.004500 0.001255 0.004882 0.000525 0.003591 0.001369 0.004709
Mitotic Spindle 0.000188 0.002654 0.003618 0.001013 0.000099 0.005071 0.001121 0.022049 0.000147 0.004001 0.002197 0.020778
None 0.026220 0.006791 0.001708 0.003323 0.002143 0.009518 0.003287 0.004938 0.015155 0.008311 0.002607 0.004841
Nuclear Periphery 0.000456 0.000448 0.000297 0.000550 0.000165 0.000716 0.000305 0.003704 0.000322 0.000597 0.000302 0.003513
Nuclear Periphery Foci 0.000132 0.000479 0.000151 0.000070 0.000099 0.000509 0.000210 0.001670 0.000117 0.000496 0.000185 0.001574
Nucleolus 0.016479 0.059937 0.053748 0.011630 0.037324 0.047373 0.023211 0.013977 0.026058 0.052937 0.036371 0.013835
Nucleus 0.913334* 0.903048* 0.907490* 0.945964* 0.951503* 0.908841* 0.941101* 0.868164* 0.930875* 0.906276* 0.926616* 0.872866*
Peroxisomes 0.000387 0.000724 0.000768 0.000539 0.000186 0.001064 0.000352 0.000379 0.000295 0.000913 0.000531 0.000389
Vacuole 0.000681 0.001114 0.000806 0.002305 0.000070 0.001206 0.000677 0.007795 0.000401 0.001165 0.000732 0.007463
Vacuole Periphery 0.000101 0.000842 0.000184 0.000254 0.000073 0.000895 0.000223 0.003305 0.000088 0.000871 0.000206 0.003121

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -14.06 -23.90 -12.27 -9.44 -4.13 -5.38 -22.35 -17.48 -12.39 -3.42 -12.97 -33.20* -20.74 -13.86 -1.79
Bud Neck -7.70 -21.61 -7.34 -4.84 -0.08 -5.17 -15.91 -21.74 -19.16 -9.52 -9.65 -25.61 -23.26 -19.34 -9.97
Bud Site -2.46 -6.85 -5.93 -3.27 -2.61 -5.39 -6.87 -9.47 -7.34 -5.54 -4.41 -9.04 -9.29 -7.02 -5.72
Cell Periphery 4.14 4.34 4.12 -0.29 -3.58 -4.82 -7.58 -11.69 0.34 -3.88 3.80 4.13 3.80 -0.19 -6.49
Cytoplasm 12.62 15.39 14.20 2.38 -2.09 -3.39 -0.40 -5.64 -2.72 -6.45 11.35 14.55 9.55 -2.16 -7.21
Cytoplasmic Foci 4.59 7.95 8.01 3.06 1.67 -3.61 1.85 -2.64 1.75 -3.02 2.07 8.03 4.22 1.89 -2.96
Eisosomes 2.77 0.19 -0.64 -7.01 -1.78 -7.80 -15.08 -16.54 -7.18 -2.41 1.82 -1.04 -0.46 -9.26 2.30
Endoplasmic Reticulum -12.44 -16.48 -9.92 -6.11 -4.10 -7.22 -12.93 -15.83 -8.84 -7.21 -13.83 -21.24 -18.89 -10.07 -6.63
Endosome -1.43 1.64 0.64 1.93 -1.84 -3.33 -3.05 -3.99 -0.95 -3.79 -3.46 1.00 -3.58 -1.55 -3.74
Golgi -2.05 -1.12 -1.19 1.73 -0.38 -2.60 -7.25 -2.78 0.12 -2.44 -3.20 -2.16 -2.59 -0.12 -2.34
Lipid Particles -0.13 3.21 3.32 2.30 0.36 -3.36 1.26 -1.61 3.03 -6.04 -1.88 3.61 2.79 3.75 -2.89
Mitochondria -2.84 -4.16 -5.60 -0.38 -2.70 -4.29 -14.82 -12.12 0.50 -8.59 -5.04 -7.65 -11.33 -0.47 -8.09
Mitotic Spindle -3.17 -3.07 -1.42 -0.89 -0.66 -4.36 -3.27 -8.93 -6.13 -8.03 -5.35 -4.25 -9.03 -6.90 -7.59
None 9.12 13.09 10.37 2.10 -1.56 -6.01 -1.61 -4.12 3.90 -2.59 5.26 12.04 9.48 4.16 -3.99
Nuclear Periphery -0.07 0.31 -2.42 -2.61 -3.65 -4.01 -4.53 -5.59 -4.16 -5.16 -2.26 -0.57 -5.23 -4.54 -5.20
Nuclear Periphery Foci -2.96 -1.58 -0.57 2.16 0.25 -2.78 -1.83 -3.23 -1.35 -2.77 -3.94 -2.34 -3.22 -1.42 -2.82
Nucleolus -16.84* -14.81 1.09 14.19 13.05 -4.04 5.25 10.09 13.66 4.55 -14.98* -7.95 6.51 19.73 12.90
Nucleus 1.91 3.27 -1.48 -2.56 -3.30 10.18 5.37 15.03 5.26 11.31 7.10 4.11 10.43 5.02 7.99
Peroxisomes -1.34 -2.27 -1.01 0.25 1.09 -3.22 -1.40 -0.74 3.08 1.05 -3.22 -2.14 0.30 3.92 4.68
Vacuole -1.85 -2.78 -5.68 -3.95 -4.60 -4.02 -4.95 -6.85 -5.20 -6.00 -3.95 -4.68 -6.66 -5.47 -5.99
Vacuole Periphery -1.87 -6.19 -2.40 1.47 -0.84 -3.77 -2.64 -4.45 -1.85 -3.99 -3.64 -3.79 -4.44 -1.76 -4.05
Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spt20

Spt20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available