Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – – 0.06 0 0.08 0 0 0 0 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0 0 0 0.06 0.05 0 0.09 0.07 0.23 0.06 0 – – 0.06 0.06 0 0 0 0 0 0 0
Nucleus 0.93 0.91 0.98 0.93 0.95 0.9 0.87 0.88 0.82 0.83 0.78 0.82 1.0 – – 0.84 0.86 0.85 0.81 0.84 0.85 0.7 0.54 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0 0 0 0 0.09 0.13 0.15 0.23 0.17 0.34 0.28 0.26 – – 0 0.06 0 0.11 0.07 0.07 0.06 0.06 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0.06 0 0 0 0 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 1 2 2 0 0 1 0 – – 0 0 0 0 0 0 1 1 0
Bud Neck 0 0 0 0 1 0 0 1 2 0 1 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 3 7 5 3 4 5 0 – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Cytoplasm 6 11 3 12 7 9 15 17 8 7 7 9 0 – – 6 2 7 0 0 2 0 1 2
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 1 0 0 0 0 0 0
Endosome 0 1 1 2 2 0 0 0 0 0 1 0 0 – – 1 1 1 2 5 0 1 1 0
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 – – 1 1 0 1 4 0 0 0 0
Mitochondria 11 24 0 5 2 20 21 17 24 19 53 17 0 – – 6 3 1 5 2 0 0 0 1
Nucleus 204 337 215 229 286 302 377 411 221 217 178 235 23 – – 90 44 71 184 322 198 22 11 37
Nuclear Periphery 1 1 1 3 1 1 6 0 1 1 2 1 0 – – 2 0 0 1 0 0 0 0 0
Nucleolus 29 9 2 9 13 31 55 70 63 45 78 80 6 – – 3 3 2 25 28 16 1 1 5
Peroxisomes 0 0 0 0 0 1 1 0 0 0 0 0 0 – – 0 0 0 1 0 0 0 0 0
SpindlePole 0 4 6 2 4 1 3 9 3 5 2 2 0 – – 1 0 0 1 4 1 0 0 0
Vac/Vac Membrane 1 9 1 1 3 3 3 5 1 2 1 1 0 – – 5 1 5 1 13 9 1 1 4
Unique Cell Count 220 369 220 247 302 337 434 469 269 260 229 285 23 107 51 84 226 386 233 33 21 54
Labelled Cell Count 254 399 229 263 319 369 485 539 330 299 327 351 29 115 56 88 226 386 233 33 21 54


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.4 7.6 6.9 7.5 6.4 6.5 6.8 5.8 6.2 5.4 6.0 6.5 – – 8.6 9.3 8.9 8.3 – 9.0
Std Deviation (1e-4) 1.0 1.1 1.6 1.6 1.5 1.3 1.6 1.6 1.3 1.4 1.2 1.2 1.0 – – 1.8 2.2 2.1 1.4 – 2.0
Intensity Change (Log2) – – – -0.14 -0.03 -0.26 -0.24 -0.16 -0.39 -0.29 -0.49 -0.35 -0.24 – – 0.17 0.28 0.23 0.12 – 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cytoplasm 2.1 0 0 1.5 1.6 0 0 0 1.3 0 – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Mitochondria 0 0 3.7 3.3 2.9 4.5 4.1 7.6 3.7 0 – – 0 0 0
Nucleus -2.5 -1.7 -3.6 -4.5 -4.3 -5.5 -5.2 -6.4 -5.5 0.7 – – -4.6 -3.6 -4.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nucleolus 1.9 2.3 4.1 5.0 5.6 7.3 6.0 9.2 8.2 0 – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
SpindlePole 0 0 0 0 0 0 -0.6 0 0 0 – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3462 2.2669 2.3166 2.2099 2.5747 2.1113 1.8325 1.8581 1.7191 1.9127 1.1155 1.5898 3.2256 2.2934 2.3135 2.1375 1.9514 2.1041
Actin 0.0112 0 0.0028 0.006 0.0002 0.0001 0.0014 0.0001 0.0011 0 0.0037 0.0027 0.0184 0 0.0119 0.0105 0.0088 0.0001
Bud 0.0006 0 0.0004 0.0003 0.0013 0.0001 0.0005 0.0001 0.0003 0 0.0003 0.0006 0.0008 0 0.0006 0.0009 0.0024 0.0002
Bud Neck 0.0027 0.0001 0.0004 0.0007 0.0026 0.0008 0.0011 0.0001 0.0002 0 0.0024 0.0028 0.0016 0.0001 0.0005 0.0144 0.0133 0.0019
Bud Periphery 0.0023 0 0.0014 0.0004 0.0034 0.0004 0.0008 0 0.0002 0 0.0007 0.001 0.0014 0 0.0008 0.0037 0.0061 0.0004
Bud Site 0.0019 0 0.0006 0.0062 0.0045 0.0002 0.0045 0.0035 0.0013 0 0.0004 0.0014 0.0029 0.0001 0.0061 0.0022 0.0098 0.0003
Cell Periphery 0.001 0 0.0004 0.0003 0.001 0.0001 0.0002 0 0 0 0.0002 0.0002 0.0003 0 0.0004 0.0023 0.0018 0.0001
Cytoplasm 0.0028 0.0001 0.0005 0.0045 0.0015 0.0002 0.0064 0.0001 0.0163 0 0.0013 0.0016 0.0281 0.0019 0.0032 0.0002 0.0007 0.0003
Cytoplasmic Foci 0.008 0 0.0009 0.005 0.0064 0.0002 0.0234 0.0026 0.0095 0 0.0001 0.0147 0.0413 0.0001 0.0123 0.0008 0.0022 0
Eisosomes 0.0002 0 0.0001 0.0002 0 0 0.0001 0 0 0 0.0001 0 0.001 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0038 0 0.0013 0.0023 0.0006 0.0008 0.0039 0 0.0015 0 0.0068 0.0008 0.0086 0.0005 0.0005 0.0009 0.0008 0
Endosome 0.0229 0 0.0055 0.0075 0.0492 0.0049 0.0272 0.0002 0.0158 0 0.0025 0.0309 0.0307 0.0015 0.0129 0.0142 0.021 0.0001
Golgi 0.0042 0 0.0026 0.0014 0.0043 0.0005 0.0038 0.0003 0.0012 0 0.0003 0.0111 0.0053 0 0.0073 0.0128 0.0327 0
Lipid Particles 0.0093 0 0.0073 0.0096 0.0022 0.001 0.017 0.0004 0.002 0 0.0003 0.0121 0.0218 0.0002 0.0082 0.0037 0.0034 0
Mitochondria 0.0096 0 0.0145 0.008 0.0058 0.0024 0.0024 0.0002 0.0008 0 0.003 0.0069 0.0069 0.0002 0.0177 0.0164 0.0074 0.0009
None 0.0028 0 0.0003 0.0024 0.0002 0.0001 0.0057 0.0001 0.0014 0 0.0002 0.0004 0.062 0.0011 0.0007 0.0001 0.0001 0.0001
Nuclear Periphery 0.0338 0.0011 0.0061 0.0046 0.0015 0.0085 0.0218 0.0005 0.0031 0.0001 0.059 0.0065 0.0837 0.0046 0.0034 0.0011 0.0016 0.0005
Nucleolus 0.0667 0.0428 0.0263 0.0332 0.1424 0.1144 0.0221 0.0237 0.0218 0.0082 0.0687 0.0594 0.0293 0.0303 0.0388 0.0264 0.0673 0.1132
Nucleus 0.7661 0.9524 0.9175 0.8963 0.7474 0.8489 0.8041 0.9658 0.9171 0.9913 0.8442 0.8222 0.576 0.9377 0.8603 0.8732 0.7957 0.8695
Peroxisomes 0.0029 0 0.0024 0.0013 0.0005 0.0001 0.0046 0.0013 0.0011 0 0.0001 0.0181 0.0074 0 0.0043 0.0004 0.0005 0
Punctate Nuclear 0.0338 0.0033 0.0035 0.0059 0.0018 0.0108 0.0437 0.0009 0.004 0.0002 0.0025 0.0035 0.0606 0.0192 0.0031 0.0014 0.0027 0.0121
Vacuole 0.0093 0 0.0031 0.0029 0.02 0.0037 0.0041 0.0001 0.001 0 0.0017 0.0022 0.0056 0.0019 0.0055 0.0104 0.0195 0.0003
Vacuole Periphery 0.004 0 0.002 0.0012 0.0032 0.0018 0.0013 0 0.0003 0 0.0015 0.0009 0.006 0.0005 0.0013 0.0036 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.8944 16.8472 16.534 17.9419 15.3702 13.9748 15.7978 19.0997 15.7776 18.5199
Translational Efficiency 0.5451 0.6187 0.7122 0.686 0.712 0.6108 0.6928 0.6394 0.7563 0.6404

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2185 1627 1270 128 1858 2047 1677 1990 4043 3674 2947 2118

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 701.36 797.77 994.44 1132.05 790.56 780.73 902.48 956.44 742.35 788.28 942.11 967.05
Standard Deviation 82.59 126.29 130.58 144.16 98.33 123.29 150.78 137.43 100.53 124.91 149.53 144.05
Intensity Change Log 2 — 0.185818 0.503729 0.690711 — -0.018051 0.191020 0.274800 — 0.081384 0.346489 0.485290

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000043 0.000462 0.001110 0.001508 0.000156 0.000356 0.000877 0.000985 0.000095 0.000403 0.000978 0.001017
Bud Neck 0.002740 0.009768 0.022002 0.020736 0.004750 0.008390 0.020280 0.045388 0.003664 0.009000 0.021022 0.043898
Bud Site 0.000667 0.001535 0.001586 0.002054 0.000226 0.001089 0.001689 0.005321 0.000465 0.001286 0.001645 0.005124
Cell Periphery 0.001136 0.000093 0.000043 0.000080 0.000026 0.000116 0.000079 0.000138 0.000626 0.000106 0.000063 0.000134
Cytoplasm 0.030244 0.003647 0.000526 0.001308 0.000993 0.003215 0.001484 0.005232 0.016801 0.003406 0.001071 0.004995
Cytoplasmic Foci 0.004645 0.001315 0.000070 0.000031 0.000102 0.001772 0.000036 0.001941 0.002557 0.001570 0.000051 0.001826
Eisosomes 0.000056 0.000028 0.000049 0.000048 0.000016 0.000027 0.000033 0.000037 0.000038 0.000027 0.000040 0.000037
Endoplasmic Reticulum 0.000799 0.003060 0.003838 0.006121 0.001516 0.002826 0.003442 0.005419 0.001128 0.002930 0.003613 0.005462
Endosome 0.000350 0.000595 0.000201 0.000214 0.000083 0.001203 0.000173 0.003244 0.000227 0.000934 0.000185 0.003061
Golgi 0.000117 0.000454 0.000142 0.000120 0.000043 0.000719 0.000109 0.001260 0.000083 0.000602 0.000123 0.001191
Lipid Particles 0.000547 0.000560 0.000141 0.000112 0.000079 0.000593 0.000057 0.000170 0.000332 0.000578 0.000093 0.000166
Mitochondria 0.000677 0.002446 0.001520 0.002019 0.000346 0.004500 0.001255 0.004882 0.000525 0.003591 0.001369 0.004709
Mitotic Spindle 0.000188 0.002654 0.003618 0.001013 0.000099 0.005071 0.001121 0.022049 0.000147 0.004001 0.002197 0.020778
None 0.026220 0.006791 0.001708 0.003323 0.002143 0.009518 0.003287 0.004938 0.015155 0.008311 0.002607 0.004841
Nuclear Periphery 0.000456 0.000448 0.000297 0.000550 0.000165 0.000716 0.000305 0.003704 0.000322 0.000597 0.000302 0.003513
Nuclear Periphery Foci 0.000132 0.000479 0.000151 0.000070 0.000099 0.000509 0.000210 0.001670 0.000117 0.000496 0.000185 0.001574
Nucleolus 0.016479 0.059937 0.053748 0.011630 0.037324 0.047373 0.023211 0.013977 0.026058 0.052937 0.036371 0.013835
Nucleus 0.913334* 0.903048* 0.907490* 0.945964* 0.951503* 0.908841* 0.941101* 0.868164* 0.930875* 0.906276* 0.926616* 0.872866*
Peroxisomes 0.000387 0.000724 0.000768 0.000539 0.000186 0.001064 0.000352 0.000379 0.000295 0.000913 0.000531 0.000389
Vacuole 0.000681 0.001114 0.000806 0.002305 0.000070 0.001206 0.000677 0.007795 0.000401 0.001165 0.000732 0.007463
Vacuole Periphery 0.000101 0.000842 0.000184 0.000254 0.000073 0.000895 0.000223 0.003305 0.000088 0.000871 0.000206 0.003121

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -14.06 -23.90 -12.27 -9.44 -4.13 -5.38 -22.35 -17.48 -12.39 -3.42 -12.97 -33.20* -20.74 -13.86 -1.79
Bud Neck -7.70 -21.61 -7.34 -4.84 -0.08 -5.17 -15.91 -21.74 -19.16 -9.52 -9.65 -25.61 -23.26 -19.34 -9.97
Bud Site -2.46 -6.85 -5.93 -3.27 -2.61 -5.39 -6.87 -9.47 -7.34 -5.54 -4.41 -9.04 -9.29 -7.02 -5.72
Cell Periphery 4.14 4.34 4.12 -0.29 -3.58 -4.82 -7.58 -11.69 0.34 -3.88 3.80 4.13 3.80 -0.19 -6.49
Cytoplasm 12.62 15.39 14.20 2.38 -2.09 -3.39 -0.40 -5.64 -2.72 -6.45 11.35 14.55 9.55 -2.16 -7.21
Cytoplasmic Foci 4.59 7.95 8.01 3.06 1.67 -3.61 1.85 -2.64 1.75 -3.02 2.07 8.03 4.22 1.89 -2.96
Eisosomes 2.77 0.19 -0.64 -7.01 -1.78 -7.80 -15.08 -16.54 -7.18 -2.41 1.82 -1.04 -0.46 -9.26 2.30
Endoplasmic Reticulum -12.44 -16.48 -9.92 -6.11 -4.10 -7.22 -12.93 -15.83 -8.84 -7.21 -13.83 -21.24 -18.89 -10.07 -6.63
Endosome -1.43 1.64 0.64 1.93 -1.84 -3.33 -3.05 -3.99 -0.95 -3.79 -3.46 1.00 -3.58 -1.55 -3.74
Golgi -2.05 -1.12 -1.19 1.73 -0.38 -2.60 -7.25 -2.78 0.12 -2.44 -3.20 -2.16 -2.59 -0.12 -2.34
Lipid Particles -0.13 3.21 3.32 2.30 0.36 -3.36 1.26 -1.61 3.03 -6.04 -1.88 3.61 2.79 3.75 -2.89
Mitochondria -2.84 -4.16 -5.60 -0.38 -2.70 -4.29 -14.82 -12.12 0.50 -8.59 -5.04 -7.65 -11.33 -0.47 -8.09
Mitotic Spindle -3.17 -3.07 -1.42 -0.89 -0.66 -4.36 -3.27 -8.93 -6.13 -8.03 -5.35 -4.25 -9.03 -6.90 -7.59
None 9.12 13.09 10.37 2.10 -1.56 -6.01 -1.61 -4.12 3.90 -2.59 5.26 12.04 9.48 4.16 -3.99
Nuclear Periphery -0.07 0.31 -2.42 -2.61 -3.65 -4.01 -4.53 -5.59 -4.16 -5.16 -2.26 -0.57 -5.23 -4.54 -5.20
Nuclear Periphery Foci -2.96 -1.58 -0.57 2.16 0.25 -2.78 -1.83 -3.23 -1.35 -2.77 -3.94 -2.34 -3.22 -1.42 -2.82
Nucleolus -16.84* -14.81 1.09 14.19 13.05 -4.04 5.25 10.09 13.66 4.55 -14.98* -7.95 6.51 19.73 12.90
Nucleus 1.91 3.27 -1.48 -2.56 -3.30 10.18 5.37 15.03 5.26 11.31 7.10 4.11 10.43 5.02 7.99
Peroxisomes -1.34 -2.27 -1.01 0.25 1.09 -3.22 -1.40 -0.74 3.08 1.05 -3.22 -2.14 0.30 3.92 4.68
Vacuole -1.85 -2.78 -5.68 -3.95 -4.60 -4.02 -4.95 -6.85 -5.20 -6.00 -3.95 -4.68 -6.66 -5.47 -5.99
Vacuole Periphery -1.87 -6.19 -2.40 1.47 -0.84 -3.77 -2.64 -4.45 -1.85 -3.99 -3.64 -3.79 -4.44 -1.76 -4.05
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spt20

Spt20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available