ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0.06 0.06 0.05 0 – 0 0 0.06 0.06 0.05 0 0 0 0 0.06 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.61 0.67 0.71 0.34 0.3 0.21 – 0.25 0.21 0.25 0.23 0.21 0.73 0.67 0.85 0.61 0.71 – 0.22 0.21 0.17 0.1 0.09 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 – 0 0 0 0 0 0
Endosome 0 0.06 0 0 0.09 0 – 0 0 0 0 0 0 0.12 0 0.22 0.06 – 0.05 0 0.06 0.06 0.07 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.34 0.25 0.33 0.77 0.57 0.86 – 0.76 0.86 0.78 0.86 0.86 0.2 0.18 0.07 0.17 0 – 0.52 0.54 0.53 0.58 0.57 0.53
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.09 0 – 0.08 0.06 0.08 0.07 0.05 0 0 0 0 0.12 – 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.1 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.17 0 – 0.09 0.09 0.07 0.08 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 3 0 0 0 0 – 0 0 0 0 0 2 2 1 0 0 – 12 8 4 3 13 15
Bud 6 6 8 20 17 17 – 24 15 37 28 32 9 6 2 0 1 – 1 3 2 1 7 8
Bud Neck 5 2 4 0 2 0 – 4 1 6 3 4 0 0 2 0 0 – 8 4 0 1 11 10
Bud Site 1 0 1 2 2 2 – 6 8 11 5 10 0 0 0 0 0 – – – – – – –
Cell Periphery 2 3 2 1 3 0 – 2 0 1 1 3 1 4 3 0 0 – 1 1 0 0 1 2
Cytoplasm 269 198 97 104 107 86 – 169 91 152 116 142 184 244 343 11 12 – 124 82 27 14 36 47
Endoplasmic Reticulum 7 1 1 1 2 5 – 1 0 0 0 3 3 2 3 1 1 – 4 2 4 3 5 5
Endosome 10 17 0 1 32 0 – 4 0 2 1 1 9 45 13 4 1 – 29 14 8 9 31 29
Golgi 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 0 – 4 0 1 2 3 6
Mitochondria 151 73 45 239 207 348 – 518 374 485 428 575 50 66 27 3 0 – 296 210 84 87 243 237
Nucleus 1 0 0 2 3 4 – 8 8 4 2 7 1 0 0 1 0 – 1 0 0 1 2 3
Nuclear Periphery 0 0 0 0 0 0 – 1 1 2 0 1 0 0 0 0 0 – 0 0 1 0 0 2
Nucleolus 1 0 0 0 0 1 – 3 0 0 1 1 0 0 0 0 0 – 1 0 0 1 2 0
Peroxisomes 1 0 0 3 17 0 – 0 0 3 0 5 0 18 1 0 0 – 2 1 3 1 6 3
SpindlePole 8 4 5 7 32 7 – 52 24 49 35 34 5 5 6 0 2 – 13 9 3 4 16 17
Vac/Vac Membrane 33 29 1 5 13 13 – 9 6 14 5 6 11 16 20 3 0 – 53 36 11 11 37 45
Unique Cell Count 438 296 137 310 362 405 679 433 620 500 665 253 365 403 18 17 571 390 159 150 426 444
Labelled Cell Count 500 336 165 386 437 483 801 528 766 625 824 275 408 421 23 17 571 390 159 150 426 444


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.4 3.0 3.1 3.7 2.9 – 2.9 2.5 2.8 2.6 2.6 4.4 4.6 4.5 5.9 5.6 – 4.3 4.7 5.1
Std Deviation (1e-4) 0.9 1.4 1.1 1.0 1.3 0.7 – 0.8 0.7 0.9 1.1 0.6 0.7 0.9 0.9 1.5 1.5 – 1.4 1.2 2.3
Intensity Change (Log2) – – – 0.04 0.3 -0.06 – -0.05 -0.28 -0.08 -0.21 -0.23 0.57 0.63 0.57 0.98 0.91 – 0.52 0.66 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1606 0.3203 0.1162 -0.2188 -0.4282 0.1614 -0.3389 -0.0878 -0.2563 -0.7575 -0.5155 -0.4577 0.9616 1.5212 1.149 1.2993 1.0961 1.1108
Actin 0.0081 0.0055 0.0073 0.0146 0.0024 0.0052 0.0201 0.0095 0.0187 0.0311 0.0103 0.0104 0.0354 0.0098 0.0106 0.0008 0.0015 0.0096
Bud 0.0007 0.0079 0.0015 0.0061 0.0046 0.0011 0.0018 0.0057 0.0038 0.0031 0.0025 0.0017 0.0009 0.0041 0.0034 0.0013 0.0027 0.0026
Bud Neck 0.0009 0.0014 0.0039 0.0011 0.0014 0.0111 0.0047 0.0013 0.0065 0.0059 0.0009 0.0119 0.0039 0.002 0.0055 0.001 0.0084 0.01
Bud Periphery 0.0015 0.0103 0.004 0.017 0.0273 0.0026 0.0055 0.0092 0.0094 0.0171 0.0063 0.007 0.0023 0.0072 0.0101 0.0024 0.0054 0.0068
Bud Site 0.0022 0.0222 0.0076 0.0103 0.0019 0.0027 0.009 0.0377 0.0186 0.0022 0.0019 0.0018 0.0068 0.0431 0.0104 0.0014 0.0113 0.0028
Cell Periphery 0.0009 0.0022 0.0028 0.0015 0.0013 0.0008 0.0055 0.0014 0.0024 0.0024 0.0005 0.0016 0.0032 0.0031 0.0055 0.0005 0.0035 0.0015
Cytoplasm 0.0677 0.0959 0.0582 0.0808 0.0599 0.0815 0.0829 0.1076 0.0788 0.0849 0.0742 0.0854 0.0857 0.1603 0.1515 0.0768 0.0773 0.1604
Cytoplasmic Foci 0.129 0.1409 0.1276 0.0944 0.1699 0.1485 0.1519 0.175 0.133 0.0956 0.1491 0.1328 0.1583 0.1242 0.1066 0.0653 0.192 0.1412
Eisosomes 0.0008 0.0016 0.007 0.0007 0.0001 0.0003 0.001 0.0008 0.0013 0.0005 0.0002 0.001 0.0017 0.0008 0.0009 0.0002 0.0002 0.0006
Endoplasmic Reticulum 0.0119 0.0066 0.0052 0.0045 0.0023 0.0035 0.0097 0.0037 0.0029 0.0065 0.0056 0.0041 0.0177 0.0149 0.0104 0.0038 0.0054 0.0138
Endosome 0.1724 0.1861 0.1349 0.1305 0.1594 0.1498 0.1995 0.1533 0.1425 0.178 0.2243 0.1477 0.1761 0.1486 0.1693 0.1577 0.2984 0.1459
Golgi 0.0374 0.0609 0.0447 0.0399 0.0516 0.0463 0.0658 0.0546 0.0468 0.0453 0.0572 0.0556 0.0401 0.0493 0.0474 0.0571 0.0577 0.0565
Lipid Particles 0.0444 0.0165 0.0248 0.008 0.0124 0.028 0.0368 0.0187 0.0184 0.0044 0.0249 0.033 0.0535 0.0085 0.0203 0.0031 0.0169 0.0169
Mitochondria 0.2138 0.2111 0.3153 0.4087 0.2796 0.3217 0.2056 0.1685 0.3256 0.3189 0.2504 0.3201 0.1089 0.1982 0.2582 0.4021 0.2538 0.2386
None 0.2412 0.169 0.1893 0.1385 0.1144 0.1367 0.1233 0.1841 0.1175 0.1639 0.1255 0.1131 0.232 0.1836 0.1388 0.187 0.007 0.1411
Nuclear Periphery 0.0065 0.0029 0.0017 0.0026 0.0007 0.0021 0.0056 0.0016 0.0018 0.0011 0.0023 0.0029 0.0073 0.0028 0.0046 0.001 0.0014 0.0013
Nucleolus 0.0013 0.0013 0.0015 0.0016 0.0006 0.0008 0.0014 0.0018 0.0019 0.0005 0.0009 0.001 0.0011 0.0007 0.0007 0.0004 0.0005 0.0004
Nucleus 0.0026 0.0024 0.0023 0.0027 0.0011 0.0031 0.0039 0.0026 0.0047 0.0013 0.0018 0.0017 0.0029 0.0018 0.0016 0.0009 0.0012 0.0012
Peroxisomes 0.0325 0.0301 0.0383 0.0146 0.0927 0.0358 0.044 0.0413 0.0449 0.0247 0.0368 0.0464 0.0361 0.015 0.0242 0.0073 0.0333 0.0328
Punctate Nuclear 0.0031 0.001 0.0016 0.0011 0.0006 0.0016 0.0033 0.0057 0.0027 0.0017 0.002 0.003 0.0042 0.0007 0.0008 0.0005 0.0006 0.0009
Vacuole 0.0156 0.0185 0.0152 0.0141 0.012 0.0111 0.0133 0.0125 0.0127 0.0075 0.0143 0.0118 0.017 0.0159 0.0122 0.0104 0.0157 0.0105
Vacuole Periphery 0.0055 0.0057 0.0053 0.0067 0.0037 0.0055 0.0053 0.0033 0.0052 0.0036 0.008 0.006 0.0047 0.0052 0.0069 0.0192 0.0058 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
206 1303 608 159 1585 1863 1619 2802 1791 3166 2227 2961

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 615.73 726.35 838.23 907.63 665.40 732.54 848.02 841.53 659.69 729.99 845.35 845.08
Standard Deviation 67.72 87.99 91.67 95.34 69.00 88.23 92.41 102.47 70.66 88.19 92.31 103.18
Intensity Change Log 2 — 0.238367 0.445048 0.559806 — 0.138686 0.349876 0.338793 — 0.187454 0.396402 0.449245

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000172 0.000743 0.001628 0.008658 0.000455 0.001211 0.003431 0.010629 0.000423 0.001019 0.002939 0.010523
Bud Neck 0.052136 0.043666 0.013004 0.021851 0.037572 0.050401 0.038425 0.066103 0.039247 0.047629 0.031485 0.063726
Bud Site 0.006726 0.027624 0.016937 0.063642 0.009937 0.023962 0.062099 0.094133 0.009568 0.025469 0.049770 0.092495
Cell Periphery 0.000491 0.000292 0.000215 0.000304 0.000226 0.000280 0.000166 0.000217 0.000256 0.000285 0.000180 0.000221
Cytoplasm 0.118777* 0.086692 0.372836* 0.122890* 0.106947* 0.083831 0.180475* 0.196960* 0.108308* 0.085008 0.232992* 0.192982*
Cytoplasmic Foci 0.368968* 0.283967* 0.111206 0.234343* 0.340903* 0.263843* 0.171474 0.120152 0.344131* 0.272125* 0.155020 0.126284
Eisosomes 0.000468 0.000212 0.000101 0.000284 0.000294 0.000358 0.000127 0.000209 0.000314 0.000298 0.000120 0.000213
Endoplasmic Reticulum 0.001196 0.000937 0.006605 0.009849 0.002003 0.000855 0.005181 0.001798 0.001910 0.000889 0.005570 0.002231
Endosome 0.045444 0.080341 0.013488 0.067879 0.075156 0.087645 0.059922 0.023030 0.071738 0.084639 0.047245 0.025438
Golgi 0.023950 0.058456 0.002869 0.010593 0.044354 0.059368 0.020551 0.019108 0.042007 0.058993 0.015724 0.018650
Lipid Particles 0.049886 0.016212 0.006741 0.012973 0.032112 0.014756 0.006947 0.003379 0.034156 0.015356 0.006891 0.003894
Mitochondria 0.159152* 0.219455* 0.002439 0.005974 0.206274* 0.255252* 0.057017 0.101441* 0.200854* 0.240519* 0.042116 0.096315
Mitotic Spindle 0.001107 0.018566 0.014000 0.039330 0.005215 0.009380 0.076603 0.058789 0.004743 0.013160 0.059511 0.057744
None 0.001138 0.001926 0.002318 0.000731 0.000858 0.000689 0.001125 0.000533 0.000890 0.001198 0.001451 0.000544
Nuclear Periphery 0.002307 0.000485 0.001725 0.000925 0.000887 0.000372 0.002008 0.000554 0.001051 0.000419 0.001931 0.000574
Nuclear Periphery Foci 0.000209 0.000298 0.002926 0.007514 0.000321 0.000211 0.001621 0.000223 0.000308 0.000247 0.001977 0.000615
Nucleolus 0.002824 0.003482 0.002090 0.002555 0.002159 0.001248 0.003044 0.000887 0.002235 0.002167 0.002784 0.000976
Nucleus 0.054823 0.030343 0.338872* 0.054291 0.031659 0.019427 0.085546 0.045832 0.034324 0.023920 0.154707* 0.046286
Peroxisomes 0.075456 0.063833 0.011951 0.106358* 0.055412 0.065961 0.069073 0.129002* 0.057718 0.065085 0.053478 0.127786*
Vacuole 0.027952 0.051202 0.076641 0.226511* 0.036790 0.050335 0.147189 0.124018 0.035774 0.050692 0.127928 0.129522
Vacuole Periphery 0.006817 0.011268 0.001406 0.002546 0.010465 0.010615 0.007976 0.003004 0.010046 0.010884 0.006182 0.002979

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.14 -5.51 -7.08 -6.53 -5.36 -2.04 -11.27 -12.31 -10.23 -7.99 -2.65 -12.11 -12.87 -11.57 -8.99
Bud Neck 1.00 4.77 3.63 5.40 -2.14 -3.83 -0.21 -9.53 -4.86 -10.31 -2.95 2.98 -8.46 -6.02 -13.52
Bud Site -7.57 -3.41 -7.92 -4.65 -6.10 -7.77 -17.74 -28.08* -20.02 -7.38 -10.98 -17.31 -28.98 -21.01 -11.47
Cell Periphery 1.77 2.38 1.84 0.33 -1.23 -1.86 3.37 1.06 2.74 -1.85 -1.08 3.62 1.99 3.34 -1.24
Cytoplasm 2.42 -14.70 -0.43 -2.69 13.08 4.27 -11.16 -16.52 -22.22 -2.68 4.90 -18.87 -16.25 -23.93 5.05
Cytoplasmic Foci 4.98 15.26 8.03 5.57 -6.63 10.32 25.87 36.70 25.94 9.88 11.05 31.31 38.20 32.20 6.46
Eisosomes 3.37 4.73 2.44 -2.31 -6.26 -1.04 7.38 4.01 2.75 -7.68 0.41 8.79 4.84 2.73 -9.18
Endoplasmic Reticulum 0.76 -8.80 -3.16 -3.27 -0.95 3.24 -6.75 0.33 -3.96 7.98 3.29 -9.16 -1.01 -5.13 8.66
Endosome -5.94 6.19 -1.63 2.98 -6.71 -2.93 5.03 17.10 20.79 13.45 -3.67 8.33 16.73 24.95 10.19
Golgi -7.54 5.82 3.85 14.67 -2.54 -4.71 10.66 12.22 16.79 0.95 -6.40 13.15 12.43 21.48 -1.77
Lipid Particles 4.99 6.43 5.41 1.63 -2.38 9.03 14.69 17.05 10.50 5.95 10.32 16.04 17.95 13.17 5.73
Mitochondria -3.22 9.46 9.26 27.28* -1.20 -5.10 20.93 15.64 22.01 -8.95 -4.83 24.41 16.62 26.20 -12.79
Mitotic Spindle -7.22 -4.43 -4.09 -2.34 -2.59 -2.79 -16.80 -18.23 -16.78 3.88 -5.58 -16.65 -18.94 -15.31 0.74
None -1.44 -3.47 1.30 2.42 6.08 1.26 -1.64 2.95 1.28 4.18 -1.42 -3.95 3.38 3.12 7.83
Nuclear Periphery 1.16 0.28 0.75 -3.27 3.37 2.43 -4.37 0.79 -3.10 6.14 2.45 -3.50 1.18 -3.25 7.31
Nuclear Periphery Foci -1.52 -5.61 -2.32 -2.25 -1.21 1.24 -8.15 0.94 -0.65 8.69 -0.13 -9.28 -1.98 -1.86 6.00
Nucleolus -0.89 0.86 -0.38 0.32 -1.09 1.90 -2.18 2.65 1.04 5.61 0.04 -1.74 2.77 3.63 5.88
Nucleus 2.80 -20.29 -0.59 -3.23 18.78 4.64 -13.68 -7.21 -12.75 9.02 4.11 -23.95 -6.58 -11.98 20.33
Peroxisomes 1.31 7.34 -0.70 -1.81 -6.24 -2.76 -2.33 -13.93 -11.42 -11.09 -2.26 2.13 -13.54 -12.60 -15.52
Vacuole -4.14 -8.24 -12.67 -11.72 -9.51 -4.78 -25.34 -29.06 -23.32 1.72 -6.40 -26.09 -31.32 -26.61 -3.18
Vacuole Periphery -3.93 5.99 4.91 12.17 -2.06 -0.18 4.40 12.33 12.64 8.02 -1.20 7.06 12.85 16.47 6.62
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
Localization
Cell Percentages mitochondrion (66%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

YOL150C

YOL150C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yol150c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available