Standard name | |
---|---|
Human Ortholog | |
Description | Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 1 | 0 | 6 | 4 | 3 | 4 | 5 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 2 | 6 | 4 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 6 | 14 | 10 | 9 | 12 | 1 | 2 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 5 | 6 | 14 | 8 | 7 | 18 | 16 | 7 | 15 | 12 | 10 | 21 | 34 | 52 | 10 | 17 | 7 | 0 | 5 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 5 | 3 | 0 | 0 | 1 | 0 | 1 | 0 |
Endosome | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 2 | 3 | 5 | 1 | 1 | 2 | 1 | 4 | 1 | 3 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 2 |
Mitochondria | 2 | 42 | 0 | 4 | 4 | 38 | 70 | 43 | 151 | 149 | 213 | 95 | 7 | 2 | 3 | 1 | 3 | 1 | 1 | 1 | 2 | 2 | 6 | 7 |
Nucleus | 172 | 252 | 359 | 348 | 308 | 551 | 490 | 509 | 436 | 442 | 427 | 377 | 251 | 230 | 304 | 204 | 122 | 125 | 142 | 237 | 320 | 75 | 95 | 88 |
Nuclear Periphery | 0 | 1 | 1 | 4 | 2 | 16 | 3 | 5 | 11 | 14 | 3 | 9 | 8 | 5 | 7 | 7 | 1 | 3 | 0 | 0 | 3 | 0 | 3 | 15 |
Nucleolus | 67 | 112 | 38 | 7 | 9 | 44 | 40 | 28 | 39 | 51 | 44 | 45 | 102 | 92 | 93 | 29 | 17 | 15 | 89 | 107 | 103 | 42 | 115 | 94 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 0 | 2 | 0 | 8 | 1 | 7 | 4 | 7 | 1 | 10 | 3 | 10 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 1 | 3 | 9 |
Vac/Vac Membrane | 6 | 11 | 12 | 5 | 10 | 14 | 11 | 17 | 4 | 5 | 15 | 10 | 4 | 9 | 13 | 22 | 10 | 21 | 8 | 20 | 21 | 35 | 89 | 78 |
Unique Cell Count | 223 | 353 | 405 | 375 | 330 | 618 | 579 | 592 | 527 | 561 | 571 | 479 | 360 | 347 | 432 | 261 | 162 | 168 | 249 | 383 | 469 | 164 | 331 | 321 |
Labelled Cell Count | 252 | 427 | 418 | 388 | 342 | 691 | 642 | 638 | 672 | 700 | 730 | 580 | 401 | 391 | 478 | 281 | 177 | 177 | 249 | 383 | 469 | 164 | 331 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 7.6 | 8.6 | 7.8 | 7.5 | 6.0 | 6.0 | 6.2 | 5.2 | 5.1 | 4.8 | 5.4 | 9.1 | 8.7 | 8.4 | 11.1 | 11.6 | 10.8 | 8.6 | 8.4 | 8.6 |
Std Deviation (1e-4) | 1.7 | 1.5 | 1.3 | 1.6 | 1.3 | 1.1 | 1.1 | 1.2 | 1.1 | 1.1 | 1.2 | 1.0 | 2.8 | 2.7 | 2.4 | 2.2 | 2.7 | 2.7 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.2 | -0.52 | -0.53 | -0.47 | -0.74 | -0.77 | -0.84 | -0.67 | 0.08 | 0.02 | -0.03 | 0.36 | 0.43 | 0.33 | -0.01 | -0.04 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 0.9 | -0.5 | 1.6 | 1.3 | -0.2 | 1.3 | 0.7 | 0.7 | 3.3 | 5.1 | 6.0 | 1.9 | 4.9 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.1 | 7.3 | 5.5 | 11.8 | 11.3 | 0 | 9.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.0 | 2.2 | 0.3 | -1.8 | -1.2 | -2.5 | -4.0 | -5.4 | -3.9 | -6.5 | -7.4 | -6.5 | -3.7 | -4.0 | -4.3 |
Nuclear Periphery | 0 | 0 | 2.9 | 0 | 0 | 2.5 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -4.5 | -3.7 | -1.3 | -1.4 | -2.9 | -1.1 | -0.2 | 0 | 0 | 6.8 | 6.2 | 4.8 | 0.7 | 0.4 | -0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.6 | 0.1 | -0.7 | -1.1 | -0.1 | -2.6 | -2.4 | -0.3 | -0.8 | -1.8 | -0.3 | 0 | 3.1 | 1.8 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0645 | 4.8449 | 4.5165 | 3.1471 | 3.2569 | 3.9287 | 6.5218 | 6.5777 | 6.3982 | 5.5991 | 5.9302 | 5.9092 | 5.0179 | 5.1157 | 4.6819 | 4.6329 | 4.8632 | 4.8489 |
Actin | 0.0104 | 0 | 0.0001 | 0 | 0.0032 | 0 | 0.0012 | 0 | 0.0041 | 0.0162 | 0.0144 | 0 | 0.005 | 0 | 0.0065 | 0.0119 | 0.0502 | 0.0046 |
Bud | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0001 |
Bud Neck | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0011 | 0.0008 | 0.0002 | 0.0004 | 0.0009 | 0.0014 | 0.0006 | 0.0015 | 0.0001 | 0.0005 | 0.0003 | 0.0016 | 0.0008 |
Bud Periphery | 0.0012 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0003 | 0.0009 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0005 | 0.0002 | 0.0012 | 0.0001 |
Bud Site | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0006 | 0.0015 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.001 | 0.0007 | 0.0012 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0008 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 |
Cytoplasm | 0.0018 | 0.0003 | 0.0003 | 0 | 0.0015 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0009 | 0 | 0.0003 | 0.0012 | 0.001 | 0.0001 |
Cytoplasmic Foci | 0.0115 | 0 | 0.0077 | 0 | 0.0012 | 0.0001 | 0.0011 | 0 | 0.0029 | 0.003 | 0.0034 | 0.0001 | 0.0033 | 0 | 0.0016 | 0.0091 | 0.0045 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0022 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0007 | 0 | 0.0012 | 0.0006 | 0.0003 | 0 | 0.0012 | 0 | 0.001 | 0.0005 | 0.0022 | 0.0003 |
Endosome | 0.0072 | 0.0001 | 0.0013 | 0 | 0.0033 | 0.0001 | 0.0053 | 0 | 0.005 | 0.0114 | 0.0035 | 0.0005 | 0.0031 | 0 | 0.0025 | 0.0057 | 0.0218 | 0.0009 |
Golgi | 0.0014 | 0 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.002 | 0 | 0.0017 | 0.0109 | 0.0029 | 0.0001 | 0.0011 | 0 | 0.0006 | 0.002 | 0.0093 | 0.0004 |
Lipid Particles | 0.0072 | 0 | 0.002 | 0 | 0.002 | 0.0015 | 0.0083 | 0 | 0.0064 | 0.006 | 0.0197 | 0.0003 | 0.0118 | 0 | 0.0047 | 0.003 | 0.0127 | 0.0002 |
Mitochondria | 0.0025 | 0.0001 | 0.0009 | 0.0002 | 0.0108 | 0.0008 | 0.0154 | 0.0001 | 0.0067 | 0.0028 | 0.0014 | 0.0004 | 0.0053 | 0.0001 | 0.0042 | 0.0005 | 0.0109 | 0.0002 |
None | 0.0015 | 0 | 0.0001 | 0 | 0.0148 | 0 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0006 | 0 | 0.0005 | 0.0031 | 0.0017 | 0.0001 |
Nuclear Periphery | 0.0081 | 0.0058 | 0.0055 | 0.0081 | 0.0204 | 0.0044 | 0.0073 | 0.0029 | 0.009 | 0.0016 | 0.0066 | 0.0002 | 0.0058 | 0.0004 | 0.0062 | 0.0023 | 0.0366 | 0.0018 |
Nucleolus | 0.4032 | 0.3397 | 0.4857 | 0.379 | 0.3842 | 0.4481 | 0.3735 | 0.4367 | 0.467 | 0.356 | 0.301 | 0.4661 | 0.3975 | 0.4125 | 0.4363 | 0.4327 | 0.4242 | 0.4796 |
Nucleus | 0.5174 | 0.6462 | 0.4915 | 0.6095 | 0.5114 | 0.5389 | 0.5684 | 0.5557 | 0.4847 | 0.5819 | 0.6131 | 0.5284 | 0.5458 | 0.5839 | 0.5267 | 0.5078 | 0.3971 | 0.5082 |
Peroxisomes | 0.0103 | 0 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0019 | 0 | 0.0035 | 0.0016 | 0.003 | 0 | 0.0032 | 0 | 0.0007 | 0.0067 | 0.0056 | 0 |
Punctate Nuclear | 0.0075 | 0.0018 | 0.0013 | 0.0015 | 0.0167 | 0.002 | 0.003 | 0.0036 | 0.0033 | 0.0029 | 0.0257 | 0.0013 | 0.0066 | 0.0013 | 0.0037 | 0.0118 | 0.0125 | 0.0021 |
Vacuole | 0.0029 | 0.0046 | 0.0009 | 0.0003 | 0.0087 | 0.001 | 0.0053 | 0.0004 | 0.0013 | 0.0005 | 0.0008 | 0.0012 | 0.003 | 0.0006 | 0.0013 | 0.0002 | 0.0019 | 0.0002 |
Vacuole Periphery | 0.0013 | 0.0008 | 0.0011 | 0.0008 | 0.0169 | 0.001 | 0.0033 | 0.0002 | 0.0009 | 0.0004 | 0.0012 | 0.0004 | 0.0013 | 0.0006 | 0.0005 | 0.0001 | 0.0029 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.8346 | 39.8672 | 34.5691 | 39.5039 | 29.8856 | 29.5481 | 36.4598 | 30.5689 | 31.7538 | 35.0856 |
Translational Efficiency | 0.7605 | 0.95 | 1.0063 | 0.7731 | 0.8622 | 0.8588 | 0.8141 | 0.8048 | 0.8333 | 0.7957 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1302 | 1289 | 1128 | 1077 | 1857 | 2249 | 1350 | 1152 | 3159 | 3538 | 2478 | 2229 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 759.92 | 1008.89 | 1134.75 | 1096.03 | 891.73 | 995.16 | 1153.65 | 1193.38 | 837.40 | 1000.16 | 1145.05 | 1146.34 |
Standard Deviation | 268.84 | 198.18 | 182.72 | 176.93 | 154.23 | 167.38 | 190.39 | 189.67 | 219.05 | 179.33 | 187.17 | 189.96 |
Intensity Change Log 2 | — | 0.408849 | 0.578455 | 0.528368 | — | 0.158322 | 0.371527 | 0.420375 | — | 0.279011 | 0.470431 | 0.471068 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000276 | 0.000486 | 0.000960 | 0.001132 | 0.000279 | 0.000382 | 0.000602 | 0.000832 | 0.000278 | 0.000420 | 0.000765 | 0.000977 |
Bud Neck | 0.002816 | 0.006864 | 0.021925 | 0.041204 | 0.003768 | 0.009457 | 0.014422 | 0.037587 | 0.003375 | 0.008512 | 0.017837 | 0.039335 |
Bud Site | 0.000359 | 0.001022 | 0.001835 | 0.008915 | 0.000293 | 0.000938 | 0.000928 | 0.003261 | 0.000320 | 0.000968 | 0.001341 | 0.005993 |
Cell Periphery | 0.000111 | 0.000054 | 0.000073 | 0.000202 | 0.000040 | 0.000084 | 0.000027 | 0.000114 | 0.000069 | 0.000073 | 0.000048 | 0.000157 |
Cytoplasm | 0.002657 | 0.000509 | 0.000884 | 0.004604 | 0.000604 | 0.000650 | 0.000192 | 0.001822 | 0.001450 | 0.000599 | 0.000507 | 0.003166 |
Cytoplasmic Foci | 0.000688 | 0.000698 | 0.000080 | 0.003025 | 0.000120 | 0.000313 | 0.000132 | 0.000680 | 0.000354 | 0.000453 | 0.000108 | 0.001813 |
Eisosomes | 0.000030 | 0.000047 | 0.000058 | 0.000043 | 0.000036 | 0.000047 | 0.000048 | 0.000047 | 0.000034 | 0.000047 | 0.000053 | 0.000045 |
Endoplasmic Reticulum | 0.002977 | 0.001870 | 0.002344 | 0.002249 | 0.002248 | 0.002454 | 0.001072 | 0.001749 | 0.002549 | 0.002241 | 0.001651 | 0.001991 |
Endosome | 0.000353 | 0.001003 | 0.000224 | 0.005732 | 0.000174 | 0.001019 | 0.000436 | 0.001262 | 0.000248 | 0.001013 | 0.000339 | 0.003421 |
Golgi | 0.000151 | 0.000384 | 0.000226 | 0.008358 | 0.000087 | 0.000282 | 0.000173 | 0.001051 | 0.000114 | 0.000320 | 0.000197 | 0.004582 |
Lipid Particles | 0.000120 | 0.000444 | 0.000098 | 0.000264 | 0.000142 | 0.000248 | 0.000072 | 0.000144 | 0.000133 | 0.000320 | 0.000084 | 0.000202 |
Mitochondria | 0.000653 | 0.001154 | 0.001491 | 0.005072 | 0.000560 | 0.002250 | 0.001343 | 0.003676 | 0.000599 | 0.001851 | 0.001410 | 0.004351 |
Mitotic Spindle | 0.000060 | 0.000896 | 0.002307 | 0.033401 | 0.000405 | 0.003318 | 0.002025 | 0.012699 | 0.000263 | 0.002436 | 0.002153 | 0.022702 |
None | 0.006151 | 0.001589 | 0.001579 | 0.003803 | 0.001917 | 0.002056 | 0.000772 | 0.001571 | 0.003662 | 0.001885 | 0.001139 | 0.002650 |
Nuclear Periphery | 0.000249 | 0.000245 | 0.000297 | 0.003356 | 0.000131 | 0.001306 | 0.000251 | 0.001810 | 0.000179 | 0.000919 | 0.000272 | 0.002557 |
Nuclear Periphery Foci | 0.000445 | 0.001226 | 0.000232 | 0.003552 | 0.000243 | 0.000862 | 0.000169 | 0.001147 | 0.000327 | 0.000994 | 0.000197 | 0.002309 |
Nucleolus | 0.181778 | 0.299332* | 0.307698* | 0.266024* | 0.220738* | 0.232495* | 0.388588* | 0.325809* | 0.204680 | 0.256846* | 0.351767* | 0.296922* |
Nucleus | 0.798165* | 0.679089* | 0.653630* | 0.591238* | 0.767084* | 0.738844* | 0.585674* | 0.596035* | 0.779894* | 0.717074* | 0.616608* | 0.593717* |
Peroxisomes | 0.000412 | 0.001392 | 0.002428 | 0.001383 | 0.000450 | 0.000593 | 0.002206 | 0.001180 | 0.000435 | 0.000884 | 0.002307 | 0.001278 |
Vacuole | 0.000977 | 0.001183 | 0.001280 | 0.009958 | 0.000308 | 0.001267 | 0.000442 | 0.004828 | 0.000583 | 0.001237 | 0.000823 | 0.007307 |
Vacuole Periphery | 0.000570 | 0.000514 | 0.000352 | 0.006484 | 0.000372 | 0.001133 | 0.000427 | 0.002698 | 0.000454 | 0.000908 | 0.000393 | 0.004527 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.77 | -16.63 | -11.20 | -8.29 | -0.79 | -3.99 | -13.41 | -14.22 | -11.54 | -6.25 | -6.77 | -20.90 | -17.01 | -13.50 | -4.13 |
Bud Neck | -6.04 | -19.32 | -21.50 | -19.09 | -10.77 | -9.00 | -16.41 | -20.77 | -17.51 | -13.62 | -10.92 | -25.13 | -29.85 | -25.68 | -17.31 |
Bud Site | -2.16 | -14.58 | -6.11 | -5.37 | -4.64 | -4.54 | -5.43 | -7.37 | -6.14 | -5.70 | -4.28 | -13.04 | -8.41 | -7.35 | -6.49 |
Cell Periphery | 2.46 | 1.58 | -0.99 | -2.35 | -1.85 | -2.40 | 1.28 | -3.55 | -1.22 | -4.27 | -0.31 | 1.77 | -2.74 | -2.44 | -3.68 |
Cytoplasm | 2.50 | 1.75 | -1.85 | -4.33 | -3.67 | -0.01 | 1.77 | -2.99 | -3.15 | -3.97 | 2.29 | 2.13 | -3.34 | -5.36 | -5.21 |
Cytoplasmic Foci | -0.19 | 1.54 | -1.78 | -1.46 | -3.60 | -0.91 | 0.14 | -1.31 | -0.63 | -1.44 | -0.49 | 1.46 | -2.41 | -1.89 | -3.80 |
Eisosomes | -6.03 | -12.79 | -5.66 | 1.57 | 7.32 | -4.41 | -6.46 | -3.13 | 0.52 | 1.11 | -7.20 | -13.50 | -5.61 | 1.39 | 5.30 |
Endoplasmic Reticulum | 4.71 | 0.58 | 0.56 | -3.67 | 0.03 | -0.96 | 6.56 | 0.62 | 1.60 | -6.51 | 1.83 | 4.27 | 0.51 | -1.28 | -3.55 |
Endosome | -1.82 | 0.50 | -4.19 | -2.98 | -4.30 | -3.26 | -0.80 | -2.42 | -0.06 | -1.45 | -3.62 | -0.66 | -4.86 | -2.66 | -4.37 |
Golgi | -1.74 | -2.12 | -3.02 | -2.85 | -2.93 | -1.53 | -4.11 | -2.93 | -2.03 | -2.57 | -2.18 | -4.02 | -3.52 | -3.22 | -3.37 |
Lipid Particles | -1.55 | -0.51 | -2.11 | 0.89 | -2.21 | -0.50 | 0.63 | 0.02 | 0.83 | -1.64 | -1.39 | 0.46 | -0.76 | 1.08 | -2.73 |
Mitochondria | -1.48 | -7.58 | -7.21 | -5.52 | -5.33 | -3.63 | -5.46 | -10.61 | -3.56 | -8.21 | -3.92 | -8.82 | -11.84 | -5.90 | -8.94 |
Mitotic Spindle | -1.83 | -5.53 | -7.98 | -7.70 | -6.98 | -3.48 | -2.17 | -5.67 | -4.42 | -4.82 | -3.93 | -4.78 | -9.75 | -8.63 | -8.47 |
None | 3.27 | 3.59 | 1.59 | -3.02 | -4.35 | -0.25 | 2.73 | 0.10 | 0.44 | -4.73 | 2.76 | 4.32 | 1.22 | -2.45 | -5.94 |
Nuclear Periphery | -0.08 | -2.53 | -7.02 | -7.04 | -6.68 | -2.79 | -3.35 | -7.71 | -1.39 | -6.99 | -2.76 | -4.41 | -10.16 | -4.39 | -9.46 |
Nuclear Periphery Foci | -1.28 | 0.39 | -2.53 | -1.29 | -2.71 | -2.44 | 1.04 | -3.18 | -0.64 | -3.51 | -2.41 | 0.71 | -3.55 | -1.61 | -3.80 |
Nucleolus | -13.06 | -11.07 | -4.53 | 6.84 | 5.84 | -1.65 | -16.51 | -6.65 | -5.51 | 7.78 | -9.31 | -19.28 | -7.53 | -0.13 | 9.87 |
Nucleus | 13.05 | 13.96 | 15.75 | 4.55 | 2.59 | 3.92 | 18.62 | 15.08 | 12.21 | -2.16 | 11.09 | 22.87 | 21.52 | 12.67 | 0.08 |
Peroxisomes | -6.09 | -12.73 | -9.31 | 0.72 | 7.31 | -2.78 | -13.31 | -10.13 | -8.35 | 7.02 | -6.70 | -18.31 | -13.57 | -4.32 | 10.08 |
Vacuole | -0.40 | -1.68 | -6.27 | -6.25 | -5.76 | -3.88 | -2.30 | -5.02 | -3.94 | -4.80 | -2.25 | -2.28 | -8.07 | -7.39 | -7.46 |
Vacuole Periphery | 0.21 | 0.81 | -4.49 | -4.71 | -4.92 | -3.72 | -0.67 | -4.50 | -2.52 | -4.39 | -2.81 | 0.40 | -6.22 | -5.09 | -6.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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