Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0.09 0.05 0.06 0 0 0.05 0 0 0 0.05 0.08 0.06 0.22 0.2 0.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.06 0.07 0.05 0.05 0 0.05 0.07 0.07 0.08 0.13 0.15 0.23 0.28 0.18 0.09 0 0 0 0 0 0
Cytoplasm 0.08 0.08 0.11 0.13 0.21 0.16 0.2 0.18 0.16 0.22 0.19 0.2 0.06 0.11 0.11 0.25 0.3 0.32 0 0 0 0 0 0
Endoplasmic Reticulum 0.83 0.92 0.79 0.76 0.49 0.63 0.42 0.33 0.44 0.28 0.38 0.31 0.88 0.76 0.68 0 0 0 0.87 0.93 0.86 0.84 0.84 0.83
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.16 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.17 0.18 0.29 0.31 0.35 0.41 0.34 0.45 0.48 0 0 0 0.12 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0 0.21 0.07 0.2 0.21 0.15 0.27 0.13 0.12 0 0.09 0.13 0.14 0.24 0.33 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 5 14 17 16 23 6 17 22 8 9 21 14 20 21 30 28 25 4 3 5 4 6 6
Bud 0 0 2 2 5 14 9 12 11 19 10 29 0 0 3 2 3 4 2 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud Site 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 – – – – – –
Cell Periphery 13 6 11 11 21 21 20 20 25 36 30 44 41 40 84 39 25 12 1 0 5 1 4 4
Cytoplasm 25 15 22 26 63 66 86 94 73 113 78 107 19 28 41 35 42 41 10 3 1 3 5 5
Endoplasmic Reticulum 250 173 158 150 149 261 184 168 205 145 159 163 272 201 246 0 2 1 292 180 183 185 231 218
Endosome 1 0 1 0 5 2 3 9 1 6 5 4 0 1 0 0 1 0 3 0 3 1 0 2
Golgi 2 1 3 9 5 3 6 0 5 4 4 5 0 10 6 13 12 20 2 0 1 3 2 3
Mitochondria 12 0 27 33 55 121 137 180 191 178 190 256 1 1 1 16 8 5 3 1 2 6 6 5
Nucleus 2 0 0 1 1 4 4 5 1 3 2 3 0 2 0 0 2 0 0 0 0 0 0 0
Nuclear Periphery 10 0 9 9 4 5 8 16 16 17 13 24 1 1 1 5 4 3 3 0 4 1 2 0
Nucleolus 0 0 0 0 0 0 1 2 0 0 0 4 0 0 0 0 1 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 26 3 6 3 63 31 89 106 70 139 55 62 6 23 46 20 34 42 7 3 2 9 6 9
Unique Cell Count 300 189 201 198 306 414 441 512 465 518 421 529 309 263 363 139 142 128 337 194 213 220 274 264
Labelled Cell Count 346 203 253 261 387 551 554 630 620 668 555 723 354 327 449 160 163 154 337 194 213 220 274 264


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 14.3 10.0 10.1 9.2 8.6 7.8 7.8 7.8 7.7 7.7 7.6 13.1 13.2 13.4 16.1 15.7 16.5 13.7 13.2 14.0
Std Deviation (1e-4) 2.4 2.3 2.2 2.0 1.5 1.6 1.6 1.7 1.6 1.7 1.8 1.6 1.9 2.1 1.9 3.9 4.3 4.0 2.0 2.3 2.7
Intensity Change (Log2) – – – 0.01 -0.11 -0.21 -0.36 -0.34 -0.36 -0.37 -0.36 -0.39 0.4 0.4 0.43 0.7 0.65 0.72 0.46 0.41 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -0.8 -0.7 -3.8 -2.1 -1.2 -3.8 -3.0 -1.7 -1.2 0.3 -0.6 3.9 3.5 3.4
Bud 0 0 1.7 0 0 0 1.9 0 2.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.6 -0.2 -0.5 -0.9 -0.1 0.7 0.8 1.3 2.8 3.3 5.4 5.8 3.6 1.4
Cytoplasm 0.7 2.8 1.7 2.7 2.4 1.6 3.3 2.4 2.9 -1.9 -0.1 0.1 3.5 4.4 4.7
Endoplasmic Reticulum -0.7 -6.7 -3.9 -8.7 -11.0 -8.2 -12.3 -9.5 -11.6 2.9 -0.6 -2.7 -14.3 -14.1 -13.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.8 0 0 0 0 0 0 0 0 0 1.5 0 3.4 3.1 4.9
Mitochondria 0.9 1.4 4.3 4.8 5.7 7.0 5.6 7.8 8.7 -6.4 -5.9 -6.9 -0.5 -2.4 -2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -2.2 0 -1.9 -0.9 -0.6 -0.8 -0.9 0 0 0 0 -0.4 -0.8 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.7 2.2 5.7 5.8 4.5 7.2 4.0 3.6 0 2.5 3.8 3.9 6.0 7.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0038 0.0002 0.0076 0.0012 0.0068 0.007 0.0125 0.006 0.0112 0.0254 0.0089 0.0056 – – – – – –
Bud 0.0001 0 0 0 0.0002 0.0001 0.0006 0.0002 0.0001 0.0008 0.0001 0 – – – – – –
Bud Neck 0.0005 0.0001 0.0004 0.0002 0.0004 0.0011 0.0007 0.0006 0.0003 0.0003 0.0002 0.0001 – – – – – –
Bud Periphery 0.0002 0.0001 0.0001 0.0001 0.0006 0.0001 0.0009 0.0003 0.0001 0.0045 0.0001 0 – – – – – –
Bud Site 0.0004 0.0002 0.0001 0.0001 0.0009 0.0001 0.0102 0.0246 0.001 0.0005 0.0001 0 – – – – – –
Cell Periphery 0.0009 0.0019 0.0006 0.0073 0.001 0.0003 0.0012 0.0056 0.0013 0.0009 0.0005 0.0003 – – – – – –
Cytoplasm 0.0187 0.0538 0.0213 0.0017 0.0073 0.0331 0.0229 0.0162 0.0105 0.0011 0.002 0.0053 – – – – – –
Cytoplasmic Foci 0.0034 0.0012 0.0007 0.0004 0.0031 0.0019 0.0179 0.0015 0.0035 0.0001 0.0004 0.0011 – – – – – –
Eisosomes 0.0001 0.0001 0.0001 0.0081 0.0004 0.0001 0.0003 0.0002 0.0012 0.001 0.0004 0 – – – – – –
Endoplasmic Reticulum 0.8895 0.9231 0.9427 0.9347 0.8131 0.9301 0.7991 0.9375 0.9467 0.9542 0.9533 0.9777 – – – – – –
Endosome 0.0191 0.0038 0.0058 0.0157 0.0287 0.0041 0.0267 0.0012 0.009 0.0013 0.0078 0.0014 – – – – – –
Golgi 0.0049 0.0005 0.0038 0.0032 0.0049 0.0022 0.0076 0.0009 0.004 0.002 0.0016 0.0005 – – – – – –
Lipid Particles 0.0088 0.0036 0.002 0.0024 0.0172 0.0029 0.0154 0.0011 0.0019 0.0004 0.0012 0.0032 – – – – – –
Mitochondria 0.0084 0.0001 0.0005 0.0013 0.0105 0.0013 0.0035 0.0002 0.0038 0.002 0.0009 0.0001 – – – – – –
None 0.0036 0.0002 0.0003 0.0006 0.006 0.0003 0.0107 0.0006 0.0003 0.0002 0.0052 0.0004 – – – – – –
Nuclear Periphery 0.0213 0.0069 0.0106 0.0174 0.0468 0.0052 0.0482 0.0015 0.0026 0.0034 0.0131 0.0019 – – – – – –
Nucleolus 0.0004 0 0 0.0002 0.007 0 0.0009 0 0 0.0001 0.0002 0 – – – – – –
Nucleus 0.0065 0.0011 0.0012 0.0013 0.0252 0.001 0.0033 0.0002 0.0002 0.0002 0.0014 0.0001 – – – – – –
Peroxisomes 0.0007 0 0.0001 0 0.0021 0.007 0.0039 0.0007 0.0002 0 0.0001 0.001 – – – – – –
Punctate Nuclear 0.004 0.0002 0.0005 0.0004 0.0088 0.0006 0.0065 0.0001 0.0001 0 0.0009 0.0002 – – – – – –
Vacuole 0.0036 0.0026 0.0013 0.0022 0.0047 0.0011 0.0037 0.001 0.0017 0.0012 0.0008 0.0008 – – – – – –
Vacuole Periphery 0.001 0.0002 0.0003 0.0014 0.0043 0.0002 0.0031 0.0001 0.0003 0.0004 0.0008 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.8042 63.2292 28.1513 39.8749 44.7066 60.0881 45.9504 33.4406 34.0668 60.7307
Translational Efficiency 1.1571 0.9197 1.2138 1.064 1.3282 1.531 1.0736 1.0907 1.0495 1.0015

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1568 1392 1616 1344 1628 2021 2622 1604 3196 3413 4238 2948

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 890.99 1180.66 1503.10 1610.01 1060.68 1116.88 1580.06 1616.29 977.43 1142.89 1550.71 1613.43
Standard Deviation 144.75 241.75 204.83 266.68 169.92 174.39 222.31 242.83 179.40 206.94 219.02 254.00
Intensity Change Log 2 — 0.406112 0.754460 0.853589 — 0.074485 0.574990 0.607697 — 0.235381 0.659701 0.725171

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003487 0.000828 0.000885 0.003469 0.000655 0.000719 0.000814 0.001876 0.002044 0.000764 0.000841 0.002602
Bud Neck 0.000749 0.000257 0.001372 0.003830 0.000415 0.000409 0.000726 0.001622 0.000579 0.000347 0.000972 0.002629
Bud Site 0.009683 0.003675 0.005580 0.020619 0.005234 0.004314 0.003670 0.014727 0.007417 0.004054 0.004398 0.017413
Cell Periphery 0.007147 0.003305 0.003017 0.015762 0.001881 0.002244 0.007202 0.007592 0.004464 0.002676 0.005606 0.011317
Cytoplasm 0.027055 0.010399 0.018345 0.015871 0.020355 0.014299 0.009891 0.022808 0.023642 0.012709 0.013114 0.019645
Cytoplasmic Foci 0.011893 0.005299 0.000593 0.004298 0.010854 0.006102 0.000594 0.007321 0.011363 0.005775 0.000594 0.005943
Eisosomes 0.000806 0.000105 0.000073 0.000219 0.000077 0.000193 0.000118 0.000108 0.000435 0.000157 0.000101 0.000158
Endoplasmic Reticulum 0.885197* 0.952032* 0.934377* 0.841194* 0.932696* 0.947543* 0.957035* 0.863865* 0.909392* 0.949374* 0.948395* 0.853529*
Endosome 0.005040 0.004832 0.006155 0.012485 0.003876 0.004326 0.002910 0.013588 0.004447 0.004533 0.004148 0.013085
Golgi 0.009211 0.003026 0.000732 0.007065 0.004624 0.004315 0.001046 0.009584 0.006874 0.003789 0.000926 0.008436
Lipid Particles 0.007706 0.002517 0.004554 0.034485 0.003223 0.003846 0.006710 0.022127 0.005422 0.003304 0.005888 0.027761
Mitochondria 0.012339 0.002075 0.000341 0.002022 0.003913 0.002101 0.000753 0.002322 0.008047 0.002090 0.000596 0.002185
Mitotic Spindle 0.000189 0.000461 0.000502 0.004690 0.000068 0.000233 0.000092 0.002104 0.000127 0.000326 0.000248 0.003283
None 0.003050 0.001558 0.000986 0.001436 0.001355 0.002447 0.001484 0.001253 0.002187 0.002084 0.001294 0.001336
Nuclear Periphery 0.000954 0.000574 0.003247 0.001120 0.001395 0.000174 0.000227 0.000524 0.001179 0.000337 0.001379 0.000796
Nuclear Periphery Foci 0.004566 0.004508 0.012854 0.012071 0.004129 0.001448 0.001379 0.010036 0.004343 0.002696 0.005754 0.010963
Nucleolus 0.000149 0.000054 0.000006 0.000031 0.000042 0.000119 0.000009 0.000030 0.000095 0.000092 0.000008 0.000031
Nucleus 0.002271 0.000487 0.000957 0.001505 0.000915 0.000796 0.000313 0.001042 0.001580 0.000670 0.000558 0.001253
Peroxisomes 0.005477 0.000605 0.000039 0.000394 0.000927 0.001144 0.000851 0.000653 0.003159 0.000924 0.000542 0.000535
Vacuole 0.001412 0.002281 0.005122 0.015459 0.001980 0.001978 0.003909 0.013137 0.001701 0.002102 0.004372 0.014196
Vacuole Periphery 0.001620 0.001123 0.000263 0.001976 0.001386 0.001248 0.000269 0.003681 0.001501 0.001197 0.000267 0.002904

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.33 3.16 -0.15 -3.79 -3.60 -0.25 -0.85 -3.26 -3.15 -2.46 3.07 2.60 -1.26 -4.90 -4.33
Bud Neck 2.80 -2.25 -6.11 -7.29 -4.55 -0.01 -1.33 -4.50 -5.54 -3.21 1.58 -2.26 -7.52 -8.94 -5.72
Bud Site 4.13 2.26 -4.56 -7.32 -6.08 0.75 1.00 -4.95 -5.43 -5.56 3.53 2.66 -6.58 -8.98 -8.36
Cell Periphery 2.99 3.67 -4.80 -7.56 -8.36 -0.61 -6.88 -6.73 -6.59 -0.35 2.61 -1.69 -7.15 -9.98 -6.43
Cytoplasm 7.88 3.25 3.91 -3.11 0.86 3.46 6.16 -1.56 -4.12 -5.99 7.96 7.10 1.41 -5.03 -4.44
Cytoplasmic Foci 4.49 9.68 5.65 0.79 -5.84 2.98 7.68 2.09 -0.96 -6.91 5.15 12.10 4.97 -0.26 -8.86
Eisosomes 3.42 3.59 2.85 -3.34 -4.91 -1.17 -1.86 -1.62 0.84 0.43 2.38 3.30 2.71 -0.05 -3.18
Endoplasmic Reticulum -9.39 -6.44 5.19 13.55 11.13 -2.70 -4.72 9.47 11.87 13.63 -8.92 -8.69 9.92 17.90 17.89
Endosome 0.32 -2.26 -7.68 -8.00 -6.37 -0.95 2.44 -9.59 -8.77 -10.52 -0.25 0.40 -12.18 -11.85 -12.60
Golgi 5.14 7.71 1.37 -3.37 -5.77 0.29 4.52 -3.14 -3.40 -6.03 3.85 8.76 -1.41 -4.59 -8.15
Lipid Particles 5.60 2.62 -9.19 -11.17 -10.19 -0.89 -3.73 -9.25 -8.94 -7.22 3.58 -0.89 -12.85 -14.22 -12.26
Mitochondria 6.49 8.18 6.24 -0.01 -2.26 2.40 4.57 1.79 -0.36 -2.74 6.94 9.25 6.32 -0.23 -3.44
Mitotic Spindle -1.04 -0.80 -3.55 -3.27 -3.17 -1.38 -1.19 -3.68 -3.29 -3.61 -1.57 -0.86 -4.87 -4.47 -4.57
None 1.78 2.69 1.76 0.17 -0.71 -2.37 -0.34 0.24 2.21 0.49 0.21 2.10 1.73 1.69 -0.11
Nuclear Periphery 1.26 -3.94 -0.74 -2.66 3.81 2.69 2.57 1.87 -5.58 -4.91 3.11 -0.75 1.30 -4.78 2.66
Nuclear Periphery Foci 0.02 -5.97 -4.62 -4.55 0.20 3.65 3.56 -4.59 -7.47 -7.41 2.61 -2.29 -6.50 -8.42 -5.00
Nucleolus 0.98 1.63 1.32 0.56 -3.66 -1.25 2.79 0.77 1.43 -2.74 -0.03 2.00 1.43 1.53 -4.35
Nucleus 2.33 1.57 0.56 -3.22 -1.78 0.22 2.91 -1.12 -1.13 -3.51 2.09 2.50 0.14 -2.83 -3.85
Peroxisomes 6.37 7.18 6.68 1.62 -4.55 -1.16 0.39 1.38 2.11 0.80 5.71 6.67 6.73 2.72 0.05
Vacuole -1.90 -7.74 -9.15 -8.46 -6.41 -0.07 -3.99 -8.21 -8.13 -6.64 -1.30 -7.81 -12.27 -11.72 -9.32
Vacuole Periphery 1.25 5.85 -0.76 -1.58 -3.81 0.40 5.20 -3.42 -3.54 -5.31 1.19 7.82 -3.28 -3.81 -6.51
Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erp4

Erp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available