Standard name | |
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Human Ortholog | |
Description | Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 5 | 14 | 17 | 16 | 23 | 6 | 17 | 22 | 8 | 9 | 21 | 14 | 20 | 21 | 30 | 28 | 25 | 4 | 3 | 5 | 4 | 6 | 6 |
Bud | 0 | 0 | 2 | 2 | 5 | 14 | 9 | 12 | 11 | 19 | 10 | 29 | 0 | 0 | 3 | 2 | 3 | 4 | 2 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 6 | 11 | 11 | 21 | 21 | 20 | 20 | 25 | 36 | 30 | 44 | 41 | 40 | 84 | 39 | 25 | 12 | 1 | 0 | 5 | 1 | 4 | 4 |
Cytoplasm | 25 | 15 | 22 | 26 | 63 | 66 | 86 | 94 | 73 | 113 | 78 | 107 | 19 | 28 | 41 | 35 | 42 | 41 | 10 | 3 | 1 | 3 | 5 | 5 |
Endoplasmic Reticulum | 250 | 173 | 158 | 150 | 149 | 261 | 184 | 168 | 205 | 145 | 159 | 163 | 272 | 201 | 246 | 0 | 2 | 1 | 292 | 180 | 183 | 185 | 231 | 218 |
Endosome | 1 | 0 | 1 | 0 | 5 | 2 | 3 | 9 | 1 | 6 | 5 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 3 | 1 | 0 | 2 |
Golgi | 2 | 1 | 3 | 9 | 5 | 3 | 6 | 0 | 5 | 4 | 4 | 5 | 0 | 10 | 6 | 13 | 12 | 20 | 2 | 0 | 1 | 3 | 2 | 3 |
Mitochondria | 12 | 0 | 27 | 33 | 55 | 121 | 137 | 180 | 191 | 178 | 190 | 256 | 1 | 1 | 1 | 16 | 8 | 5 | 3 | 1 | 2 | 6 | 6 | 5 |
Nucleus | 2 | 0 | 0 | 1 | 1 | 4 | 4 | 5 | 1 | 3 | 2 | 3 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 10 | 0 | 9 | 9 | 4 | 5 | 8 | 16 | 16 | 17 | 13 | 24 | 1 | 1 | 1 | 5 | 4 | 3 | 3 | 0 | 4 | 1 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 26 | 3 | 6 | 3 | 63 | 31 | 89 | 106 | 70 | 139 | 55 | 62 | 6 | 23 | 46 | 20 | 34 | 42 | 7 | 3 | 2 | 9 | 6 | 9 |
Unique Cell Count | 300 | 189 | 201 | 198 | 306 | 414 | 441 | 512 | 465 | 518 | 421 | 529 | 309 | 263 | 363 | 139 | 142 | 128 | 337 | 194 | 213 | 220 | 274 | 264 |
Labelled Cell Count | 346 | 203 | 253 | 261 | 387 | 551 | 554 | 630 | 620 | 668 | 555 | 723 | 354 | 327 | 449 | 160 | 163 | 154 | 337 | 194 | 213 | 220 | 274 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 14.3 | 10.0 | 10.1 | 9.2 | 8.6 | 7.8 | 7.8 | 7.8 | 7.7 | 7.7 | 7.6 | 13.1 | 13.2 | 13.4 | 16.1 | 15.7 | 16.5 | 13.7 | 13.2 | 14.0 |
Std Deviation (1e-4) | 2.4 | 2.3 | 2.2 | 2.0 | 1.5 | 1.6 | 1.6 | 1.7 | 1.6 | 1.7 | 1.8 | 1.6 | 1.9 | 2.1 | 1.9 | 3.9 | 4.3 | 4.0 | 2.0 | 2.3 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.11 | -0.21 | -0.36 | -0.34 | -0.36 | -0.37 | -0.36 | -0.39 | 0.4 | 0.4 | 0.43 | 0.7 | 0.65 | 0.72 | 0.46 | 0.41 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | -0.8 | -0.7 | -3.8 | -2.1 | -1.2 | -3.8 | -3.0 | -1.7 | -1.2 | 0.3 | -0.6 | 3.9 | 3.5 | 3.4 |
Bud | 0 | 0 | 1.7 | 0 | 0 | 0 | 1.9 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.6 | -0.2 | -0.5 | -0.9 | -0.1 | 0.7 | 0.8 | 1.3 | 2.8 | 3.3 | 5.4 | 5.8 | 3.6 | 1.4 |
Cytoplasm | 0.7 | 2.8 | 1.7 | 2.7 | 2.4 | 1.6 | 3.3 | 2.4 | 2.9 | -1.9 | -0.1 | 0.1 | 3.5 | 4.4 | 4.7 |
Endoplasmic Reticulum | -0.7 | -6.7 | -3.9 | -8.7 | -11.0 | -8.2 | -12.3 | -9.5 | -11.6 | 2.9 | -0.6 | -2.7 | -14.3 | -14.1 | -13.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 3.4 | 3.1 | 4.9 |
Mitochondria | 0.9 | 1.4 | 4.3 | 4.8 | 5.7 | 7.0 | 5.6 | 7.8 | 8.7 | -6.4 | -5.9 | -6.9 | -0.5 | -2.4 | -2.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | -2.2 | 0 | -1.9 | -0.9 | -0.6 | -0.8 | -0.9 | 0 | 0 | 0 | 0 | -0.4 | -0.8 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 5.7 | 2.2 | 5.7 | 5.8 | 4.5 | 7.2 | 4.0 | 3.6 | 0 | 2.5 | 3.8 | 3.9 | 6.0 | 7.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0038 | 0.0002 | 0.0076 | 0.0012 | 0.0068 | 0.007 | 0.0125 | 0.006 | 0.0112 | 0.0254 | 0.0089 | 0.0056 | – | – | – | – | – | – |
Bud | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0 | – | – | – | – | – | – |
Bud Neck | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0011 | 0.0007 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0045 | 0.0001 | 0 | – | – | – | – | – | – |
Bud Site | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0102 | 0.0246 | 0.001 | 0.0005 | 0.0001 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0009 | 0.0019 | 0.0006 | 0.0073 | 0.001 | 0.0003 | 0.0012 | 0.0056 | 0.0013 | 0.0009 | 0.0005 | 0.0003 | – | – | – | – | – | – |
Cytoplasm | 0.0187 | 0.0538 | 0.0213 | 0.0017 | 0.0073 | 0.0331 | 0.0229 | 0.0162 | 0.0105 | 0.0011 | 0.002 | 0.0053 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0034 | 0.0012 | 0.0007 | 0.0004 | 0.0031 | 0.0019 | 0.0179 | 0.0015 | 0.0035 | 0.0001 | 0.0004 | 0.0011 | – | – | – | – | – | – |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0081 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0012 | 0.001 | 0.0004 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.8895 | 0.9231 | 0.9427 | 0.9347 | 0.8131 | 0.9301 | 0.7991 | 0.9375 | 0.9467 | 0.9542 | 0.9533 | 0.9777 | – | – | – | – | – | – |
Endosome | 0.0191 | 0.0038 | 0.0058 | 0.0157 | 0.0287 | 0.0041 | 0.0267 | 0.0012 | 0.009 | 0.0013 | 0.0078 | 0.0014 | – | – | – | – | – | – |
Golgi | 0.0049 | 0.0005 | 0.0038 | 0.0032 | 0.0049 | 0.0022 | 0.0076 | 0.0009 | 0.004 | 0.002 | 0.0016 | 0.0005 | – | – | – | – | – | – |
Lipid Particles | 0.0088 | 0.0036 | 0.002 | 0.0024 | 0.0172 | 0.0029 | 0.0154 | 0.0011 | 0.0019 | 0.0004 | 0.0012 | 0.0032 | – | – | – | – | – | – |
Mitochondria | 0.0084 | 0.0001 | 0.0005 | 0.0013 | 0.0105 | 0.0013 | 0.0035 | 0.0002 | 0.0038 | 0.002 | 0.0009 | 0.0001 | – | – | – | – | – | – |
None | 0.0036 | 0.0002 | 0.0003 | 0.0006 | 0.006 | 0.0003 | 0.0107 | 0.0006 | 0.0003 | 0.0002 | 0.0052 | 0.0004 | – | – | – | – | – | – |
Nuclear Periphery | 0.0213 | 0.0069 | 0.0106 | 0.0174 | 0.0468 | 0.0052 | 0.0482 | 0.0015 | 0.0026 | 0.0034 | 0.0131 | 0.0019 | – | – | – | – | – | – |
Nucleolus | 0.0004 | 0 | 0 | 0.0002 | 0.007 | 0 | 0.0009 | 0 | 0 | 0.0001 | 0.0002 | 0 | – | – | – | – | – | – |
Nucleus | 0.0065 | 0.0011 | 0.0012 | 0.0013 | 0.0252 | 0.001 | 0.0033 | 0.0002 | 0.0002 | 0.0002 | 0.0014 | 0.0001 | – | – | – | – | – | – |
Peroxisomes | 0.0007 | 0 | 0.0001 | 0 | 0.0021 | 0.007 | 0.0039 | 0.0007 | 0.0002 | 0 | 0.0001 | 0.001 | – | – | – | – | – | – |
Punctate Nuclear | 0.004 | 0.0002 | 0.0005 | 0.0004 | 0.0088 | 0.0006 | 0.0065 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0002 | – | – | – | – | – | – |
Vacuole | 0.0036 | 0.0026 | 0.0013 | 0.0022 | 0.0047 | 0.0011 | 0.0037 | 0.001 | 0.0017 | 0.0012 | 0.0008 | 0.0008 | – | – | – | – | – | – |
Vacuole Periphery | 0.001 | 0.0002 | 0.0003 | 0.0014 | 0.0043 | 0.0002 | 0.0031 | 0.0001 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.8042 | 63.2292 | 28.1513 | 39.8749 | 44.7066 | 60.0881 | 45.9504 | 33.4406 | 34.0668 | 60.7307 |
Translational Efficiency | 1.1571 | 0.9197 | 1.2138 | 1.064 | 1.3282 | 1.531 | 1.0736 | 1.0907 | 1.0495 | 1.0015 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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