Standard name
Human Ortholog
Description DDHD domain-containing phospholipase A1; mitochondrial matrix enzyme with sn-1-specific activity, hydrolyzing cardiolipin, PE, PC, PG and PA; implicated in remodeling of mitochondrial phospholipids; antagonistically regulated by Aft1p and Aft2p; in humans, mutations in DDHD1 and DDHD2 genes cause specific types of hereditary spastic paraplegia, while DDL1-defective yeast share similar phenotypes such as mitochondrial dysfunction and defects in lipid metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.05 – 0.05 0 0 0 0 – – – 0 0 0 0 0.05 0
Bud Neck 0 0 0.11 0.08 0.09 0.05 0.08 0.09 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.62 0.75 0.89 0.8 0.76 0.5 0.49 0.58 0.33 – 0.35 0.35 0.84 0.89 0.88 – – – 0.27 0.26 0.18 0.19 0.16 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.07 – – – 0.06 0.05 0 0.05 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.2 0.22 0 0.07 0.12 0.45 0.41 0.28 0.69 – 0.69 0.57 0.13 0 0 – – – 0.4 0.47 0.56 0.53 0.48 0.5
Nucleus 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0.07 0 0 0.07 0.08 0.08 0.08 0.12 0.08 – 0.08 0.13 0 0 0 – – – 0.06 0 0 0 0.06 0.05
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0.05 0.06 0.05 0.05 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 9 6 0 0 3 3 0 – 0 1 0 0 0 – – – 3 19 12 4 17 19
Bud 5 8 1 8 5 2 17 9 13 – 13 9 13 4 2 – – – 1 2 2 2 18 9
Bud Neck 6 2 42 17 25 10 29 20 7 – 5 12 1 0 0 – – – 1 6 5 6 11 12
Bud Site 1 0 0 0 0 2 2 2 10 – 7 3 0 0 0 – – – – – – – – –
Cell Periphery 3 2 14 4 0 2 0 1 1 – 0 2 2 0 2 – – – 1 3 8 2 5 7
Cytoplasm 131 262 349 169 204 109 174 130 94 – 94 134 248 367 413 – – – 65 118 86 39 64 70
Endoplasmic Reticulum 0 4 5 2 1 1 0 0 2 – 1 1 1 0 0 – – – 6 4 5 4 7 6
Endosome 6 6 1 0 1 2 0 0 1 – 0 2 4 22 31 – – – 15 24 20 10 13 24
Golgi 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 – – – 1 2 0 1 1 4
Mitochondria 42 76 1 14 33 100 145 63 199 – 187 217 40 9 21 – – – 96 214 265 108 199 218
Nucleus 10 4 0 2 1 5 11 5 7 – 1 4 0 1 0 – – – 1 0 0 0 1 1
Nuclear Periphery 13 3 0 0 0 0 1 0 0 – 0 1 0 0 0 – – – 1 0 1 0 0 0
Nucleolus 8 0 0 1 0 0 0 0 0 – 1 1 0 0 0 – – – 2 0 0 0 2 0
Peroxisomes 4 4 0 0 0 0 0 0 0 – 0 2 5 16 19 – – – 2 1 2 2 9 5
SpindlePole 15 7 13 15 21 18 30 28 23 – 22 51 7 12 12 – – – 13 16 12 7 25 20
Vac/Vac Membrane 10 9 2 1 8 2 5 4 4 – 5 6 3 12 11 – – – 11 27 25 10 25 24
Unique Cell Count 211 350 393 210 270 220 356 225 287 272 379 297 412 469 239 460 475 204 416 441
Labelled Cell Count 254 387 437 240 299 253 417 265 361 336 446 324 443 511 239 460 475 204 416 441


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 3.7 4.7 4.2 5.0 3.6 4.0 4.3 3.2 – 3.2 3.7 3.9 4.0 4.1 – – – 4.1 3.8 4.4
Std Deviation (1e-4) 11.2 0.5 0.7 0.9 1.6 0.9 1.4 1.3 1.0 – 1.0 1.0 1.2 0.9 1.1 – – – 2.4 1.0 2.2
Intensity Change (Log2) – – – -0.16 0.08 -0.39 -0.22 -0.14 -0.54 – -0.58 -0.36 -0.28 -0.24 -0.21 – – – -0.19 -0.33 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.853 -0.5696 -0.7948 -0.6996 -1.178 -0.6225 -0.3626 0.0194 -0.2472 0.4521 -0.6657 -0.1505 -0.2802 -0.5067 -0.4889 -0.576 -0.6834 -0.6182
Actin 0.0273 0.0223 0.0182 0.0292 0.0251 0.012 0.0307 0.0066 0.0248 0.037 0.0034 0.0167 0.0283 0.0182 0.0251 0.0078 0.0064 0.0081
Bud 0.0009 0.003 0.0043 0.0037 0.0009 0.0016 0.0078 0.0052 0.0061 0.0059 0.006 0.0038 0.0022 0.0037 0.0025 0.0027 0.0032 0.001
Bud Neck 0.003 0.0055 0.0044 0.0061 0.0013 0.0176 0.0066 0.0019 0.0037 0.0173 0.0024 0.0114 0.0029 0.0021 0.0053 0.0019 0.0052 0.0042
Bud Periphery 0.0024 0.0056 0.005 0.0067 0.0022 0.0077 0.0073 0.0058 0.0158 0.0091 0.0153 0.0079 0.0088 0.005 0.005 0.0132 0.0084 0.0023
Bud Site 0.0077 0.0304 0.0168 0.0067 0.0011 0.0013 0.0128 0.0326 0.0229 0.0317 0.0028 0.0022 0.0101 0.0463 0.0216 0.0019 0.0063 0.0015
Cell Periphery 0.0021 0.0022 0.0029 0.0011 0.0003 0.0018 0.0016 0.0031 0.0019 0.0009 0.0006 0.0011 0.0014 0.0024 0.0041 0.0013 0.0011 0.0007
Cytoplasm 0.0616 0.0967 0.0718 0.1641 0.052 0.0715 0.0854 0.1184 0.1226 0.0442 0.1209 0.0974 0.0693 0.0682 0.0659 0.0915 0.0434 0.0506
Cytoplasmic Foci 0.1116 0.1351 0.1468 0.0968 0.1382 0.1269 0.1609 0.1778 0.1444 0.1248 0.1329 0.1614 0.1301 0.1564 0.124 0.1236 0.1618 0.139
Eisosomes 0.0014 0.0023 0.0025 0.0009 0.0012 0.0006 0.0008 0.001 0.0015 0.0005 0.0002 0.0003 0.0042 0.002 0.0016 0.0014 0.0004 0.0005
Endoplasmic Reticulum 0.0106 0.007 0.0084 0.005 0.0015 0.0051 0.006 0.0042 0.004 0.0023 0.0034 0.0038 0.0062 0.0057 0.0066 0.0034 0.0071 0.0032
Endosome 0.1555 0.1812 0.1481 0.1862 0.1198 0.1659 0.206 0.1436 0.126 0.0613 0.1699 0.1342 0.1404 0.1212 0.1029 0.1367 0.1574 0.1011
Golgi 0.0385 0.051 0.0358 0.0355 0.0307 0.0461 0.0561 0.0527 0.0508 0.0305 0.0493 0.0593 0.0398 0.0657 0.0416 0.0332 0.0557 0.0411
Lipid Particles 0.0712 0.0384 0.0405 0.0095 0.0341 0.0451 0.0418 0.0077 0.014 0.0564 0.0147 0.0219 0.0262 0.0187 0.0293 0.0136 0.023 0.0219
Mitochondria 0.1626 0.2259 0.1453 0.1358 0.3083 0.2833 0.1804 0.2441 0.2527 0.3065 0.3284 0.28 0.2138 0.254 0.2714 0.3091 0.1985 0.3441
None 0.2636 0.1205 0.2818 0.2642 0.171 0.1427 0.102 0.1285 0.1342 0.2084 0.0869 0.1069 0.2322 0.1804 0.1847 0.1831 0.222 0.1812
Nuclear Periphery 0.0133 0.0023 0.0025 0.0029 0.0174 0.0026 0.0046 0.0009 0.0014 0.0013 0.0011 0.0039 0.0033 0.0014 0.0042 0.0012 0.0223 0.0017
Nucleolus 0.002 0.0011 0.0027 0.0008 0.0015 0.001 0.0025 0.001 0.0013 0.0093 0.0006 0.0005 0.0015 0.0011 0.0018 0.0012 0.0018 0.0009
Nucleus 0.0086 0.0023 0.003 0.0036 0.0074 0.0015 0.0047 0.0017 0.0019 0.0035 0.0011 0.0012 0.003 0.0011 0.0026 0.0014 0.005 0.0013
Peroxisomes 0.0292 0.033 0.0378 0.0178 0.0671 0.0459 0.0484 0.0503 0.0544 0.0292 0.04 0.0748 0.0493 0.0353 0.0829 0.0594 0.0462 0.084
Punctate Nuclear 0.008 0.0017 0.0067 0.0017 0.005 0.003 0.0084 0.0013 0.0023 0.0119 0.0009 0.0033 0.0081 0.0033 0.003 0.0014 0.0033 0.004
Vacuole 0.0134 0.0245 0.0109 0.0158 0.0086 0.0106 0.0201 0.0087 0.0092 0.0051 0.0113 0.0052 0.0154 0.0056 0.0095 0.0071 0.0133 0.0047
Vacuole Periphery 0.0055 0.0083 0.0038 0.0058 0.0052 0.0062 0.0052 0.0031 0.004 0.0028 0.0079 0.0028 0.0037 0.0022 0.0044 0.004 0.0083 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.678 4.1329 3.7483 7.1302 4.9952 9.2413 8.1253 6.8942 7.6064 7.1007
Translational Efficiency 0.5322 0.9742 0.9645 0.6191 0.6309 0.6487 0.6287 0.9012 0.8434 0.8262

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
612 927 177 108 822 1700 1579 405 1434 2627 1756 513

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 627.05 742.87 990.05 888.04 596.80 692.44 824.43 832.85 609.71 710.24 841.12 844.47
Standard Deviation 74.50 89.57 107.54 108.81 88.94 79.06 101.89 99.73 84.42 86.35 113.96 104.17
Intensity Change Log 2 — 0.244529 0.658921 0.502044 — 0.214442 0.466149 0.480809 — 0.229936 0.568129 0.491728

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000351 0.001254 0.007398 0.010188 0.000393 0.001784 0.000575 0.006641 0.000375 0.001597 0.001263 0.007387
Bud Neck 0.078680 0.050537 0.014488 0.014685 0.040910 0.049734 0.009334 0.018409 0.057029 0.050017 0.009853 0.017625
Bud Site 0.026245 0.034803 0.031072 0.081652 0.013121 0.028291 0.010881 0.073594 0.018722 0.030589 0.012916 0.075291
Cell Periphery 0.000295 0.000158 0.000370 0.000261 0.000198 0.000320 0.000187 0.000220 0.000239 0.000263 0.000206 0.000229
Cytoplasm 0.271773* 0.115897* 0.174305* 0.189509* 0.213332* 0.095721 0.378153* 0.257888* 0.238273* 0.102841* 0.357606* 0.243492*
Cytoplasmic Foci 0.309482* 0.286288* 0.071915 0.127316 0.339384* 0.300850* 0.061992 0.117662 0.326623* 0.295712* 0.062992 0.119694
Eisosomes 0.000362 0.000196 0.000169 0.000166 0.000194 0.000324 0.000089 0.000173 0.000265 0.000279 0.000097 0.000172
Endoplasmic Reticulum 0.001390 0.000901 0.025276 0.005191 0.001404 0.000333 0.003026 0.003230 0.001398 0.000533 0.005269 0.003643
Endosome 0.031628 0.079747 0.032695 0.037983 0.058165 0.075639 0.009579 0.019494 0.046840 0.077089 0.011909 0.023386
Golgi 0.024055 0.039423 0.002222 0.007655 0.020474 0.069854 0.000656 0.007747 0.022002 0.059116 0.000814 0.007728
Lipid Particles 0.019969 0.007961 0.014887 0.003619 0.030581 0.013847 0.006520 0.005360 0.026052 0.011770 0.007363 0.004994
Mitochondria 0.079466 0.126551* 0.002333 0.009300 0.071944 0.182026* 0.001963 0.002977 0.075154 0.162450* 0.002000 0.004308
Mitotic Spindle 0.002945 0.035023 0.062419 0.077919 0.004397 0.010865 0.012170 0.048922 0.003777 0.019390 0.017235 0.055027
None 0.003503 0.001023 0.001856 0.009888 0.006234 0.000766 0.006975 0.001292 0.005069 0.000857 0.006459 0.003102
Nuclear Periphery 0.000257 0.000502 0.005181 0.001691 0.000882 0.000198 0.002723 0.000895 0.000615 0.000305 0.002970 0.001062
Nuclear Periphery Foci 0.000199 0.000282 0.013864 0.001840 0.000567 0.000148 0.001648 0.001722 0.000410 0.000195 0.002880 0.001747
Nucleolus 0.002918 0.002372 0.003696 0.000949 0.009048 0.001428 0.002728 0.000748 0.006432 0.001761 0.002825 0.000790
Nucleus 0.072923 0.034904 0.264818* 0.095601 0.093508 0.017169 0.399150* 0.124183 0.084722 0.023427 0.385610* 0.118166
Peroxisomes 0.019608 0.094831 0.030490 0.042571 0.040084 0.106624* 0.004968 0.084581 0.031345 0.102462* 0.007540 0.075737
Vacuole 0.048503 0.078305 0.237236* 0.277908* 0.048930 0.037546 0.085965 0.222869* 0.048747 0.051929 0.101213 0.234456*
Vacuole Periphery 0.005447 0.009040 0.003311 0.004108 0.006252 0.006533 0.000720 0.001393 0.005909 0.007418 0.000981 0.001965

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.13 -3.86 -2.39 -2.09 -0.23 -3.02 -2.01 -4.86 -2.56 -4.43 -4.04 -4.52 -5.05 -3.45 -3.85
Bud Neck 4.61 11.34 12.35 10.03 1.63 -2.20 8.85 6.61 10.93 -2.67 1.90 15.19 12.79 13.95 -2.43
Bud Site -2.24 -1.18 -2.56 -1.82 -2.01 -6.92 1.38 -5.69 -3.15 -6.18 -5.98 3.32 -5.67 -3.47 -6.89
Cell Periphery 4.06 -2.06 1.94 -1.44 4.04 -2.50 1.43 1.27 3.08 -0.11 -0.64 2.44 2.94 2.52 0.77
Cytoplasm 13.50 6.98 4.15 -2.53 -0.61 13.03 -11.94 -1.96 -11.62 7.16 18.95 -9.26 0.89 -11.26 7.67
Cytoplasmic Foci 2.21 23.51 13.99 13.27 -2.06 3.97 33.94 23.37 24.00 -5.88 4.28 42.26 27.58 27.53 -6.27
Eisosomes 3.58 4.01 4.21 0.89 0.80 -6.19 10.12 2.58 7.81 -6.39 -0.51 8.67 5.10 6.92 -6.17
Endoplasmic Reticulum 2.25 -9.17 -4.53 -5.11 7.15 3.32 -8.03 -5.66 -12.76 1.95 4.21 -12.66 -7.86 -12.37 5.24
Endosome -10.62 0.21 2.08 10.01 1.86 -3.77 13.11 10.16 16.97 -1.99 -9.12 14.28 9.96 20.23 -1.90
Golgi -4.04 9.75 7.14 10.92 -0.82 -14.95 11.84 7.28 20.27 -2.04 -14.70 15.58 9.79 22.40 -2.30
Lipid Particles 5.61 2.30 7.83 4.88 5.76 6.06 9.79 10.17 6.54 1.70 7.65 11.20 12.43 7.38 3.49
Mitochondria -4.56 11.89 10.39 15.86 -0.58 -13.78 13.33 13.28 29.31* -0.42 -13.91 17.88 17.48 33.64 -0.73
Mitotic Spindle -8.28 -4.42 -3.39 -1.07 0.47 -3.93 -4.92 -4.98 -3.94 -3.19 -9.00 -6.88 -6.08 -3.30 -3.17
None 3.89 2.81 -0.52 -1.17 -1.01 6.84 0.60 5.56 -3.56 4.56 7.90 -0.13 2.71 -1.95 2.48
Nuclear Periphery -3.06 -7.95 -2.70 -2.07 4.99 4.57 -13.11 -3.19 -10.36 11.21 3.28 -18.70 -5.50 -8.50 11.58
Nuclear Periphery Foci -2.27 -7.03 -6.56 -6.22 6.00 6.64 -5.62 -6.26 -8.52 0.76 5.19 -8.89 -8.70 -10.05 3.80
Nucleolus 0.45 -1.30 3.01 4.84 3.50 5.26 4.40 5.68 2.15 5.67 5.15 3.92 6.29 4.42 6.70
Nucleus 6.71 -9.45 -1.48 -3.80 6.65 13.28 -34.07 -5.28 -14.06 21.09 15.19 -39.24 -6.15 -13.95 23.25
Peroxisomes -14.45 -0.83 -0.40 8.43 0.27 -13.59 12.46 -1.36 6.66 -6.25 -19.74 12.40 -2.16 8.50 -6.27
Vacuole -6.19 -13.62 -12.60 -11.40 -4.92 2.95 -13.63 -18.76 -19.90 -13.32 -1.62 -18.03 -22.38 -22.08 -15.03
Vacuole Periphery -3.27 3.38 2.92 5.21 0.71 -0.54 10.61 9.43 12.13 -0.65 -2.61 12.47 9.89 13.01 -0.63
Standard name
Human Ortholog
Description DDHD domain-containing phospholipase A1; mitochondrial matrix enzyme with sn-1-specific activity, hydrolyzing cardiolipin, PE, PC, PG and PA; implicated in remodeling of mitochondrial phospholipids; antagonistically regulated by Aft1p and Aft2p; in humans, mutations in DDHD1 and DDHD2 genes cause specific types of hereditary spastic paraplegia, while DDL1-defective yeast share similar phenotypes such as mitochondrial dysfunction and defects in lipid metabolism
Localization
Cell Percentages mitochondrion (28%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ddl1

Ddl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ddl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available