Standard name | |
---|---|
Human Ortholog | |
Description | Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 0 | 4 | 11 | 6 | 9 | 14 | 11 | 13 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 7 | 5 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 2 | 19 | 9 | 5 | 8 | 14 | 20 | 29 | 23 | 12 | 26 | 9 | 12 | 10 | 9 | 17 | 20 | 0 | 0 | 3 | 0 | 0 | 0 |
Cytoplasm | 169 | 143 | 221 | 172 | 197 | 169 | 175 | 198 | 180 | 170 | 114 | 162 | 299 | 262 | 343 | 211 | 313 | 314 | 164 | 128 | 229 | 156 | 287 | 280 |
Endoplasmic Reticulum | 1 | 1 | 13 | 1 | 7 | 7 | 27 | 36 | 34 | 49 | 19 | 36 | 3 | 5 | 2 | 9 | 12 | 9 | 1 | 2 | 5 | 1 | 5 | 2 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | 1 | 12 | 19 | 13 | 44 | 73 | 49 | 64 | 89 | 72 | 93 | 0 | 0 | 0 | 4 | 5 | 3 | 1 | 1 | 2 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 1 | 1 | 2 | 1 | 2 | 2 | 2 | 2 | 0 | 0 | 1 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 5 | 3 | 3 | 0 | 2 | 12 | 15 | 15 | 19 | 21 | 1 | 2 | 3 | 8 | 7 | 6 | 1 | 1 | 1 | 0 | 1 | 3 |
Unique Cell Count | 174 | 146 | 244 | 185 | 217 | 210 | 259 | 282 | 287 | 296 | 212 | 298 | 304 | 268 | 347 | 227 | 326 | 331 | 176 | 138 | 251 | 167 | 311 | 300 |
Labelled Cell Count | 182 | 148 | 270 | 208 | 227 | 233 | 304 | 327 | 340 | 369 | 250 | 361 | 314 | 283 | 360 | 244 | 359 | 356 | 176 | 138 | 251 | 167 | 311 | 300 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 15.7 | 13.5 | 12.4 | 14.0 | 13.8 | 13.5 | 14.3 | 14.0 | 13.3 | 13.0 | 13.6 | 14.5 | 14.5 | 14.4 | 20.8 | 22.1 | 22.7 | 13.5 | 16.4 | 19.6 |
Std Deviation (1e-4) | 2.5 | 3.2 | 3.4 | 2.9 | 3.1 | 3.2 | 3.5 | 3.6 | 3.6 | 3.7 | 3.8 | 4.3 | 3.2 | 2.9 | 3.0 | 6.6 | 6.4 | 6.7 | 3.3 | 4.6 | 6.0 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.04 | 0.02 | 0.0 | 0.08 | 0.05 | -0.03 | -0.06 | 0.01 | 0.1 | 0.1 | 0.09 | 0.62 | 0.71 | 0.75 | -0.01 | 0.28 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.3 | 0 | 0 | 3.4 | 3.6 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.2 | -2.6 | -1.8 | -1.1 | -0.3 | 0.9 | 0 | -0.9 | 0.4 | -2.6 | -1.6 | -2.7 | -1.8 | -1.2 | -0.8 |
Cytoplasm | 0.9 | 0.1 | -3.1 | -6.3 | -5.8 | -7.4 | -8.6 | -8.9 | -9.2 | 4.1 | 3.5 | 4.7 | 0.9 | 2.6 | 2.0 |
Endoplasmic Reticulum | -2.8 | -1.1 | -1.0 | 2.1 | 2.9 | 2.6 | 4.1 | 1.5 | 2.7 | -3.0 | -2.1 | -3.6 | -0.7 | -1.0 | -1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.1 | 0.5 | 5.2 | 7.0 | 4.5 | 5.7 | 7.5 | 8.0 | 7.7 | -3.9 | -3.7 | 0 | -1.9 | -2.4 | -3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.4 | 1.9 | 1.8 | 3.3 | 2.7 | 0 | 0 | 0 | 1.0 | 0.1 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.6529 | 11.2739 | 10.6754 | 8.9884 | 9.396 | 9.885 | 8.1045 | 10.6985 | 10.0061 | 8.8633 | 8.996 | 9.6 | 7.7736 | 11.9207 | 10.8444 | 7.8578 | 11.2836 | 9.9689 |
Actin | 0.004 | 0.001 | 0.0076 | 0.0096 | 0.0008 | 0.0006 | 0.0087 | 0.0002 | 0.0046 | 0.0006 | 0.0007 | 0.0043 | 0.0269 | 0.0003 | 0.0037 | 0.0023 | 0.0013 | 0.0016 |
Bud | 0.0009 | 0.0005 | 0.0013 | 0.001 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0012 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0011 |
Bud Neck | 0.0032 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0012 | 0.0011 | 0.0003 | 0.0003 | 0.0009 | 0.0004 | 0.0009 | 0.0019 | 0.0001 | 0.0019 | 0.0013 | 0.0004 | 0.0036 |
Bud Periphery | 0.001 | 0.0001 | 0.001 | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0004 |
Bud Site | 0.0024 | 0.0018 | 0.0011 | 0.0047 | 0.0002 | 0.0002 | 0.0044 | 0.001 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0031 | 0.0002 | 0.0025 | 0.0003 | 0.0005 | 0.0002 |
Cell Periphery | 0.0009 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0001 |
Cytoplasm | 0.848 | 0.968 | 0.9268 | 0.8141 | 0.9516 | 0.9278 | 0.8181 | 0.9746 | 0.924 | 0.8539 | 0.8324 | 0.9307 | 0.8075 | 0.9596 | 0.9231 | 0.8529 | 0.9588 | 0.9273 |
Cytoplasmic Foci | 0.0154 | 0.0058 | 0.0084 | 0.0261 | 0.0119 | 0.0085 | 0.029 | 0.0038 | 0.0092 | 0.0194 | 0.0148 | 0.0065 | 0.0124 | 0.0079 | 0.006 | 0.0094 | 0.0109 | 0.0093 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0523 | 0.0045 | 0.0156 | 0.0353 | 0.0078 | 0.0284 | 0.0237 | 0.0032 | 0.0135 | 0.012 | 0.0062 | 0.0104 | 0.066 | 0.0086 | 0.0267 | 0.0207 | 0.0055 | 0.0172 |
Endosome | 0.0251 | 0.0086 | 0.0149 | 0.0405 | 0.0171 | 0.0198 | 0.0467 | 0.0061 | 0.0153 | 0.0647 | 0.0653 | 0.0174 | 0.0324 | 0.0168 | 0.0174 | 0.0418 | 0.01 | 0.0241 |
Golgi | 0.0039 | 0.0011 | 0.0019 | 0.0106 | 0.0017 | 0.0025 | 0.0079 | 0.0004 | 0.0046 | 0.0127 | 0.0129 | 0.0065 | 0.0067 | 0.0008 | 0.0008 | 0.0018 | 0.0011 | 0.0035 |
Lipid Particles | 0.0014 | 0.0001 | 0.0005 | 0.0044 | 0.0001 | 0.0002 | 0.0131 | 0.0001 | 0.0022 | 0.001 | 0.0374 | 0.0037 | 0.0093 | 0.0001 | 0.0008 | 0.0009 | 0.0003 | 0.0001 |
Mitochondria | 0.0029 | 0.0003 | 0.0009 | 0.0056 | 0.0003 | 0.0034 | 0.0028 | 0.0002 | 0.0088 | 0.0056 | 0.0105 | 0.0033 | 0.0025 | 0.0003 | 0.0016 | 0.0024 | 0.0016 | 0.0056 |
None | 0.0102 | 0.0014 | 0.0062 | 0.0008 | 0.0019 | 0.001 | 0.0034 | 0.0009 | 0.0016 | 0.0009 | 0.0011 | 0.0006 | 0.0049 | 0.0007 | 0.0027 | 0.0027 | 0.0026 | 0.0007 |
Nuclear Periphery | 0.0059 | 0.0004 | 0.0012 | 0.0021 | 0.0007 | 0.001 | 0.0047 | 0.0007 | 0.0012 | 0.0036 | 0.0018 | 0.004 | 0.0061 | 0.0008 | 0.0021 | 0.0237 | 0.0006 | 0.0009 |
Nucleolus | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0009 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0016 | 0 | 0 |
Nucleus | 0.0054 | 0.0009 | 0.0009 | 0.0013 | 0.0008 | 0.0008 | 0.0044 | 0.0017 | 0.0011 | 0.0017 | 0.0015 | 0.0019 | 0.0019 | 0.0007 | 0.0011 | 0.0143 | 0.0008 | 0.0009 |
Peroxisomes | 0.0007 | 0 | 0.0026 | 0.0224 | 0.0001 | 0.0001 | 0.008 | 0 | 0.001 | 0.001 | 0.0026 | 0.0008 | 0.0049 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0001 | 0.0026 | 0.0001 | 0.0004 | 0.0077 | 0.0001 | 0.0012 | 0.0002 | 0.0003 | 0.0033 | 0.0032 | 0.0001 | 0.0003 | 0.0042 | 0.0005 | 0.0001 |
Vacuole | 0.0122 | 0.0042 | 0.007 | 0.0143 | 0.0033 | 0.0025 | 0.0119 | 0.0058 | 0.0087 | 0.0168 | 0.0092 | 0.0037 | 0.0059 | 0.0023 | 0.0065 | 0.0151 | 0.0033 | 0.0024 |
Vacuole Periphery | 0.0015 | 0.0004 | 0.0009 | 0.0026 | 0.0006 | 0.0007 | 0.0015 | 0.0005 | 0.0009 | 0.0036 | 0.0024 | 0.0009 | 0.0012 | 0.0004 | 0.0011 | 0.0031 | 0.001 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.3364 | 41.8329 | 46.2904 | 38.8337 | 13.8378 | 12.308 | 66.2285 | 73.1224 | 57.1232 | 23.3079 |
Translational Efficiency | 0.8972 | 0.7459 | 0.6472 | 0.6871 | 0.5961 | 0.3373 | 0.67 | 0.6073 | 0.57 | 0.4508 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
195 | 161 | 1762 | 929 | 1205 | 2257 | 763 | 294 | 1400 | 2418 | 2525 | 1223 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2775.68 | 1329.59 | 1361.06 | 1679.22 | 1252.06 | 1204.95 | 1541.92 | 1882.16 | 1464.28 | 1213.25 | 1415.71 | 1728.01 |
Standard Deviation | 1217.06 | 304.88 | 201.39 | 497.61 | 253.93 | 190.92 | 267.49 | 510.05 | 734.93 | 202.92 | 238.37 | 508.08 |
Intensity Change Log 2 | — | -1.061860 | -1.028111 | -0.725050 | — | -0.055330 | 0.300424 | 0.588086 | — | -0.668247 | -0.472436 | -0.177534 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001381 | 0.000363 | 0.000199 | 0.001332 | 0.000270 | 0.000410 | 0.000406 | 0.002563 | 0.000425 | 0.000407 | 0.000261 | 0.001628 |
Bud Neck | 0.001660 | 0.000745 | 0.000580 | 0.002437 | 0.000469 | 0.000584 | 0.001188 | 0.004564 | 0.000635 | 0.000594 | 0.000764 | 0.002948 |
Bud Site | 0.033604 | 0.037285 | 0.008233 | 0.030326 | 0.022480 | 0.015850 | 0.012165 | 0.046621 | 0.024029 | 0.017277 | 0.009421 | 0.034244 |
Cell Periphery | 0.119125* | 0.000586 | 0.000168 | 0.004242 | 0.000553 | 0.000176 | 0.000181 | 0.008635 | 0.017068 | 0.000203 | 0.000172 | 0.005298 |
Cytoplasm | 0.223313* | 0.824552* | 0.921983* | 0.840824* | 0.844329* | 0.824933* | 0.865347* | 0.768242* | 0.757830* | 0.824907* | 0.904869* | 0.823376* |
Cytoplasmic Foci | 0.034356 | 0.088678 | 0.022142 | 0.023485 | 0.082284 | 0.091542 | 0.036119 | 0.032212 | 0.075608 | 0.091351 | 0.026365 | 0.025583 |
Eisosomes | 0.000026 | 0.000004 | 0.000003 | 0.000008 | 0.000005 | 0.000006 | 0.000004 | 0.000016 | 0.000008 | 0.000006 | 0.000003 | 0.000010 |
Endoplasmic Reticulum | 0.010924 | 0.002743 | 0.000525 | 0.000908 | 0.004922 | 0.002556 | 0.000704 | 0.000280 | 0.005758 | 0.002569 | 0.000579 | 0.000757 |
Endosome | 0.006186 | 0.005772 | 0.001225 | 0.002302 | 0.003816 | 0.006373 | 0.003394 | 0.003418 | 0.004146 | 0.006333 | 0.001881 | 0.002570 |
Golgi | 0.199284* | 0.009030 | 0.000991 | 0.004754 | 0.009513 | 0.009384 | 0.002341 | 0.006378 | 0.035946 | 0.009360 | 0.001399 | 0.005144 |
Lipid Particles | 0.059381 | 0.001288 | 0.001268 | 0.013402 | 0.001181 | 0.000602 | 0.001866 | 0.027212 | 0.009287 | 0.000648 | 0.001449 | 0.016722 |
Mitochondria | 0.003047 | 0.000023 | 0.000032 | 0.000189 | 0.000360 | 0.001196 | 0.000026 | 0.000344 | 0.000734 | 0.001118 | 0.000030 | 0.000226 |
Mitotic Spindle | 0.008131 | 0.000646 | 0.000641 | 0.001695 | 0.001007 | 0.002139 | 0.001919 | 0.003743 | 0.002000 | 0.002040 | 0.001027 | 0.002187 |
None | 0.004844 | 0.000484 | 0.001114 | 0.000361 | 0.000391 | 0.000309 | 0.000480 | 0.000449 | 0.001011 | 0.000321 | 0.000922 | 0.000382 |
Nuclear Periphery | 0.003065 | 0.000017 | 0.000027 | 0.000073 | 0.000056 | 0.000031 | 0.000069 | 0.000057 | 0.000475 | 0.000030 | 0.000040 | 0.000069 |
Nuclear Periphery Foci | 0.023439 | 0.001289 | 0.001691 | 0.002387 | 0.000961 | 0.000207 | 0.002786 | 0.003953 | 0.004092 | 0.000279 | 0.002022 | 0.002764 |
Nucleolus | 0.006561 | 0.000019 | 0.000049 | 0.000086 | 0.000030 | 0.000070 | 0.000065 | 0.000070 | 0.000940 | 0.000067 | 0.000054 | 0.000082 |
Nucleus | 0.019795 | 0.001796 | 0.014646 | 0.005499 | 0.002641 | 0.003089 | 0.011146 | 0.003876 | 0.005031 | 0.003003 | 0.013588 | 0.005109 |
Peroxisomes | 0.000992 | 0.000438 | 0.000131 | 0.000616 | 0.000232 | 0.000642 | 0.000267 | 0.001235 | 0.000338 | 0.000629 | 0.000172 | 0.000765 |
Vacuole | 0.084021 | 0.019698 | 0.023958 | 0.062689 | 0.014167 | 0.035536 | 0.058744 | 0.084549 | 0.023897 | 0.034481 | 0.034470 | 0.067944 |
Vacuole Periphery | 0.156865* | 0.004545 | 0.000395 | 0.002383 | 0.010335 | 0.004364 | 0.000784 | 0.001583 | 0.030745 | 0.004376 | 0.000513 | 0.002191 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.13 | 3.54 | 0.02 | -2.34 | -2.64 | -1.08 | -1.38 | -3.38 | -3.12 | -3.17 | 0.17 | 2.36 | -3.34 | -3.27 | -3.75 |
Bud Neck | 3.88 | 4.51 | -3.55 | -8.94 | -10.23 | -2.45 | -9.14 | -7.59 | -7.41 | -5.73 | 0.83 | -4.31 | -12.49 | -12.83 | -10.93 |
Bud Site | -0.37 | 4.32 | 0.54 | 1.13 | -9.59 | 4.00 | 5.55 | -4.50 | -5.83 | -6.39 | 4.01 | 8.79 | -4.20 | -7.79 | -11.39 |
Cell Periphery | 10.82 | 10.87 | 10.48 | -4.27 | -5.02 | 5.13 | 7.04 | -6.29 | -6.60 | -6.60 | 8.99 | 9.01 | 6.02 | -7.32 | -7.39 |
Cytoplasm | -23.98 | -34.32 | -28.72 | -0.17 | 10.82 | 2.65 | 0.61 | 5.87 | 4.80 | 5.42 | -7.18 | -15.15 | -4.70 | 2.13 | 11.10 |
Cytoplasmic Foci | -6.05 | 2.49 | 2.19 | 7.88 | -0.47 | -2.08 | 10.58 | 8.08 | 10.42 | 0.40 | -3.85 | 14.47 | 13.66 | 20.12 | 0.34 |
Eisosomes | 5.03 | 5.27 | 3.96 | -4.87 | -6.95 | -2.57 | -0.14 | -4.01 | -3.32 | -3.94 | 1.47 | 5.65 | -2.38 | -3.76 | -7.33 |
Endoplasmic Reticulum | 2.84 | 4.80 | 4.62 | 1.00 | -1.12 | 2.40 | 5.07 | 5.63 | 4.16 | 1.36 | 3.42 | 6.70 | 6.34 | 3.34 | -0.72 |
Endosome | 0.30 | 7.28 | 5.39 | 3.08 | -3.64 | -4.95 | 0.93 | 0.43 | 2.70 | 0.00 | -4.52 | 7.28 | 3.73 | 7.27 | -1.90 |
Golgi | 13.58 | 14.24 | 13.96 | 2.87 | -4.80 | 0.14 | 9.48 | 2.50 | 2.16 | -3.09 | 9.56 | 13.05 | 11.34 | 4.29 | -5.54 |
Lipid Particles | 7.48 | 7.48 | 5.71 | -8.82 | -9.25 | 4.11 | -3.68 | -6.90 | -7.05 | -6.64 | 7.14 | 6.30 | -4.51 | -11.94 | -11.22 |
Mitochondria | 3.52 | 3.51 | 3.31 | -1.27 | -1.18 | -2.72 | 3.67 | 0.25 | 2.48 | -1.33 | -1.22 | 4.85 | 2.98 | 3.12 | -1.69 |
Mitotic Spindle | 2.26 | 2.19 | 1.70 | -1.88 | -1.60 | -2.72 | -2.18 | -1.59 | -0.91 | -0.89 | -0.11 | 1.38 | -0.73 | -0.70 | -1.72 |
None | 2.06 | 1.80 | 2.12 | 0.40 | 2.94 | 1.33 | -0.92 | -0.44 | -0.76 | -0.16 | 2.30 | 0.42 | 1.86 | -0.73 | 2.62 |
Nuclear Periphery | 3.08 | 3.06 | 3.02 | -1.83 | -1.19 | 0.79 | -1.20 | -0.42 | -2.20 | 1.50 | 3.11 | 2.95 | 2.78 | -1.57 | -0.78 |
Nuclear Periphery Foci | 4.58 | 4.45 | 4.31 | -3.05 | -2.72 | 3.93 | -5.81 | -6.51 | -8.64 | -1.95 | 5.29 | 2.40 | 1.39 | -13.76 | -2.98 |
Nucleolus | 1.99 | 1.98 | 1.97 | -3.60 | -1.84 | -1.43 | -7.20 | -4.43 | -0.17 | 0.06 | 1.89 | 1.91 | 1.86 | -0.52 | -1.68 |
Nucleus | 3.44 | 0.58 | 2.53 | -8.60 | 11.98 | -2.10 | -13.24 | -4.14 | -3.43 | 8.76 | 2.66 | -11.61 | -1.28 | -7.33 | 14.27 |
Peroxisomes | 1.64 | 2.56 | 1.11 | -2.25 | -9.25 | -5.37 | -1.98 | -3.03 | -1.66 | -2.84 | -3.42 | 3.05 | -4.26 | -1.11 | -6.66 |
Vacuole | 8.32 | 7.98 | 1.73 | -8.91 | -9.46 | -9.30 | -13.14 | -8.91 | -6.66 | -2.05 | -4.45 | -8.45 | -12.97 | -10.40 | -7.80 |
Vacuole Periphery | 11.08 | 11.45 | 11.30 | 1.48 | -2.96 | 5.03 | 8.42 | 7.31 | 5.56 | -2.05 | 10.34 | 11.98 | 11.16 | 4.08 | -3.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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