Standard name
Human Ortholog
Description Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0 0 0.05 0.09 0.07 0 0.05 0 0 0.05 0.05 0.08 0.07 0.06 0.06 0.12 0.07 0 0.07 0 0 0 0
Bud 0 0 0.05 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.48 0.49 0.35 0.64 0.35 0.52 0.35 0.26 0.34 0.34 0.28 0.44 0.64 0.59 0.59 0.32 0.39 0.45 0.64 0.51 0.55 0.76 0.78 0.74
Cytoplasm 0.77 0.76 0.91 0.91 0.82 0.78 0.75 0.81 0.66 0.71 0.64 0.57 0.73 0.74 0.82 0.76 0.62 0.59 0.13 0.25 0.23 0.11 0.08 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0.05 0.12 0.21 0.11 0.34 0.26 0.4 0.38 0 0 0 0.05 0.06 0.07 0.06 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 4 4 1 5 6 4 9 2 4 4 6 31 39 35 20 27 12 11 6 1 9 9 13
Bud 10 2 5 2 3 2 6 3 5 1 1 1 7 8 3 2 4 8 2 0 0 0 1 4
Bud Neck 0 0 2 0 0 0 0 0 0 1 0 0 0 0 1 5 3 0 6 7 6 8 11 26
Bud Site 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 106 47 32 14 19 47 47 45 44 37 24 55 264 309 366 104 85 81 172 53 51 223 282 356
Cytoplasm 172 73 83 20 45 71 101 138 86 77 54 71 302 391 511 250 137 106 35 26 21 33 28 44
Endoplasmic Reticulum 1 0 0 0 0 1 3 2 2 1 1 2 4 2 4 18 19 6 10 1 3 3 13 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 0 0 1 2 2
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 5 1 0 2 3 0
Mitochondria 17 3 2 0 3 11 29 18 44 28 34 47 6 3 2 17 13 12 17 3 3 5 5 7
Nucleus 1 0 0 0 0 0 0 1 1 0 1 1 0 0 0 2 0 1 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 1
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 1 0 1 2 5 5 2 0 0 0 0 0 1
Vac/Vac Membrane 0 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0 3 0 2 2 1 1 0 4
Unique Cell Count 223 96 91 22 55 91 135 170 131 108 85 125 412 526 623 327 220 179 269 104 94 295 364 481
Labelled Cell Count 330 130 128 37 75 138 191 216 184 149 120 185 616 753 927 427 299 228 269 104 94 295 364 481


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.2 5.1 5.3 5.5 5.0 4.6 4.9 5.2 5.1 5.1 5.5 6.9 6.3 6.4 5.9 7.1 7.1 6.1 6.5 6.9
Std Deviation (1e-4) 0.6 0.9 1.1 1.1 1.1 0.7 0.8 0.8 0.9 0.9 1.1 1.4 0.9 1.0 1.0 1.1 1.5 1.2 0.9 1.4 1.2
Intensity Change (Log2) – – – 0.06 0.11 -0.02 -0.14 -0.06 0.05 0.01 0.01 0.12 0.44 0.33 0.35 0.23 0.49 0.49 0.26 0.36 0.45

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0.7 0 0 0 0 0 0 1.1 1.0 0 0.6 2.1 0.8
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.4 -0.1 2.2 0 0 -0.2 -0.1 -1.0 1.3 5.1 4.2 4.2 -0.6 0.6 1.6
Cytoplasm 0 -1.7 -2.5 -3.1 -2.1 -4.4 -3.5 -4.4 -5.5 -3.6 -3.5 -2.2 -3.1 -5.1 -5.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.6 0 0 5.7 4.7 6.2 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5391 6.6225 6.089 5.7286 5.8933 6.3404 3.573 4.5136 4.2146 3.8972 3.7564 4.1851 5.7488 6.7233 6.021 6.2242 5.4283 6.3104
Actin 0.0378 0.0035 0.0098 0.0071 0.0121 0.0113 0.0484 0.0026 0.0235 0.0268 0.0232 0.0074 0.0578 0.0078 0.0129 0.0076 0.0382 0.0062
Bud 0.0021 0.0004 0.001 0.0005 0.0003 0.0009 0.0014 0.0001 0.0004 0.0003 0.0003 0.0006 0.0012 0.0002 0.0002 0.0001 0.0012 0.0004
Bud Neck 0.0068 0.0013 0.0048 0.0114 0.0048 0.021 0.0099 0.0015 0.0044 0.002 0.0077 0.008 0.0126 0.0018 0.0015 0.0022 0.002 0.0026
Bud Periphery 0.0088 0.0013 0.0102 0.0055 0.0023 0.0077 0.007 0.0007 0.0028 0.0041 0.0034 0.0065 0.0064 0.0011 0.0023 0.0022 0.0036 0.0044
Bud Site 0.0127 0.0258 0.0193 0.0037 0.0036 0.0036 0.0198 0.0295 0.0359 0.0027 0.001 0.0015 0.0158 0.0242 0.0045 0.0015 0.0053 0.0008
Cell Periphery 0.3971 0.3872 0.4633 0.3836 0.4807 0.4942 0.5095 0.5376 0.4889 0.5681 0.5541 0.6601 0.5532 0.5426 0.5823 0.6915 0.5262 0.664
Cytoplasm 0.2916 0.5355 0.4212 0.4897 0.43 0.4061 0.2736 0.3793 0.3897 0.3402 0.281 0.2745 0.2488 0.4036 0.3365 0.2675 0.3057 0.3002
Cytoplasmic Foci 0.0234 0.0009 0.0038 0.001 0.0037 0.004 0.0218 0.0026 0.0035 0.0088 0.0053 0.0034 0.0128 0.0025 0.0078 0.0026 0.0107 0.0002
Eisosomes 0.0064 0.0013 0.001 0.0037 0.0005 0.0021 0.0031 0.0021 0.002 0.0013 0.0025 0.0009 0.0155 0.0007 0.0071 0.0008 0.0008 0.0007
Endoplasmic Reticulum 0.0148 0.0054 0.0076 0.0179 0.0035 0.0078 0.0123 0.0025 0.0033 0.0067 0.0079 0.0044 0.0117 0.0032 0.0075 0.0057 0.0042 0.0075
Endosome 0.0448 0.0008 0.0136 0.0058 0.006 0.0064 0.0207 0.0001 0.0087 0.0066 0.0153 0.0014 0.0054 0.0005 0.0037 0.0024 0.008 0.0013
Golgi 0.0126 0.0006 0.0045 0.007 0.0054 0.0078 0.0133 0.0004 0.0055 0.0107 0.0071 0.002 0.0052 0.0012 0.0027 0.0023 0.0051 0.0011
Lipid Particles 0.0262 0.0011 0.0021 0.007 0.0082 0.0013 0.0166 0.0014 0.0017 0.0055 0.0091 0.0011 0.0125 0.0014 0.0065 0.0076 0.0069 0.0006
Mitochondria 0.0274 0.0096 0.0139 0.0444 0.0045 0.0162 0.0037 0.0001 0.0013 0.0025 0.03 0.0013 0.0033 0.0001 0.0024 0.0008 0.0012 0.0012
None 0.02 0.0221 0.0101 0.0019 0.028 0.005 0.0142 0.0254 0.0239 0.0046 0.0331 0.0245 0.0036 0.0014 0.0089 0.0008 0.0112 0.0012
Nuclear Periphery 0.0094 0.0003 0.0038 0.0013 0.0003 0.0002 0.0036 0.0002 0.0004 0.0007 0.0053 0.0002 0.002 0.0002 0.0012 0.0003 0.0083 0.002
Nucleolus 0.0033 0.0001 0.0002 0.0002 0.0001 0.0001 0.0014 0.0001 0.0001 0.0001 0.0003 0.0001 0.0009 0 0.0003 0.0001 0.0033 0.0002
Nucleus 0.0236 0.0003 0.0008 0.0004 0.0001 0.0003 0.0028 0.0001 0.0002 0.0001 0.0009 0.0002 0.0046 0.0001 0.0003 0.0001 0.0143 0.0029
Peroxisomes 0.0065 0 0.0016 0.0003 0.0041 0.0003 0.008 0.0128 0.002 0.0056 0.0045 0.0003 0.0163 0.0066 0.0046 0.0021 0.0074 0
Punctate Nuclear 0.0061 0.0001 0.0006 0.0001 0.0003 0.0001 0.0036 0.0001 0.0001 0.0003 0.0063 0.0001 0.0053 0.0002 0.0051 0.0005 0.0345 0.0003
Vacuole 0.0159 0.0023 0.0063 0.0061 0.0014 0.0036 0.005 0.0007 0.0013 0.0022 0.0014 0.0016 0.0046 0.0008 0.0016 0.0014 0.0016 0.002
Vacuole Periphery 0.0027 0.0001 0.0006 0.0013 0.0002 0.0001 0.0005 0 0.0002 0.0001 0.0004 0 0.0004 0 0.0001 0.0001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.2305 29.7061 33.5657 35.3846 29.376 27.1736 36.3501 35.2458 31.1444 36.2575
Translational Efficiency 0.8765 0.9767 0.785 0.7889 0.7778 0.8998 0.7396 0.6061 0.8885 0.7274

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1030 1310 1518 1370 1253 2302 147 1089 2283 3612 1665 2459

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 709.37 762.49 1034.95 972.49 834.09 824.45 1117.93 975.39 777.82 801.98 1042.28 973.77
Standard Deviation 83.70 129.44 121.88 115.53 101.01 109.06 125.02 113.34 112.30 120.60 124.41 114.57
Intensity Change Log 2 — 0.104180 0.544951 0.455145 — -0.016771 0.422555 0.225776 — 0.040079 0.480100 0.335737

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002589 0.015583 0.029142 0.065788 0.007004 0.018553 0.051276 0.053399 0.005012 0.017476 0.031096 0.060302
Bud Neck 0.053358 0.045247 0.029770 0.040775 0.033732 0.064062 0.019592 0.022850 0.042587 0.057238 0.028872 0.032837
Bud Site 0.037492 0.116627 0.173655 0.185094 0.079599 0.088755 0.179640 0.141536 0.060602 0.098863 0.174183 0.165804
Cell Periphery 0.048261 0.029281 0.121307 0.098215 0.099403 0.039418 0.082730 0.059755 0.076330 0.035742 0.117901 0.081183
Cytoplasm 0.606574 0.542965 0.351016 0.370917 0.654663 0.605260 0.222775 0.415517 0.632967 0.582666 0.339694 0.390669
Cytoplasmic Foci 0.033242 0.097367 0.015992 0.009100 0.029121 0.041601 0.051836 0.025291 0.030980 0.061826 0.019156 0.016270
Eisosomes 0.029874 0.011802 0.014947 0.035884 0.021914 0.031490 0.017856 0.028197 0.025505 0.024350 0.015204 0.032480
Endoplasmic Reticulum 0.005504 0.010072 0.082624 0.010829 0.009884 0.005348 0.060459 0.010458 0.007908 0.007062 0.080667 0.010665
Endosome 0.001336 0.014660 0.001697 0.003267 0.002116 0.005106 0.003434 0.003281 0.001764 0.008571 0.001851 0.003273
Golgi 0.004162 0.010840 0.002198 0.003367 0.008271 0.010112 0.001887 0.002305 0.006417 0.010376 0.002171 0.002896
Lipid Particles 0.004293 0.008656 0.006277 0.002199 0.003578 0.005439 0.009231 0.002989 0.003901 0.006605 0.006538 0.002549
Mitochondria 0.002607 0.003845 0.000833 0.002783 0.001859 0.004576 0.000122 0.000711 0.002197 0.004311 0.000770 0.001865
Mitotic Spindle 0.000227 0.000192 0.000341 0.003573 0.000200 0.000210 0.000311 0.003934 0.000212 0.000203 0.000338 0.003733
None 0.005792 0.002794 0.001206 0.000513 0.001593 0.001492 0.000163 0.000302 0.003487 0.001964 0.001114 0.000419
Nuclear Periphery 0.000169 0.000141 0.000287 0.000113 0.000085 0.000070 0.000210 0.000114 0.000123 0.000096 0.000280 0.000114
Nuclear Periphery Foci 0.000491 0.000784 0.003405 0.000876 0.001243 0.000382 0.008384 0.001782 0.000903 0.000528 0.003844 0.001277
Nucleolus 0.000292 0.000318 0.000077 0.000054 0.000152 0.000174 0.000037 0.000038 0.000216 0.000226 0.000073 0.000047
Nucleus 0.142554 0.029051 0.112069 0.063143 0.023898 0.039238 0.071821 0.049885 0.077431 0.035543 0.108516 0.057271
Peroxisomes 0.002939 0.005592 0.000804 0.001321 0.002719 0.005955 0.001279 0.002703 0.002819 0.005823 0.000846 0.001933
Vacuole 0.018000 0.052713 0.052217 0.100749 0.018516 0.032189 0.216866 0.174695 0.018283 0.039633 0.066753 0.133497
Vacuole Periphery 0.000241 0.001471 0.000136 0.001441 0.000450 0.000571 0.000092 0.000258 0.000356 0.000897 0.000132 0.000917

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -12.06 -19.34 -22.53 -16.56 -11.38 -11.83 -8.42 -14.51 -10.15 -0.03 -17.12 -19.32 -26.21 -18.97 -11.05
Bud Neck 2.28 7.79 4.19 2.11 -4.34 -10.73 5.86 5.79 17.75 -0.99 -6.38 7.27 5.65 13.21 -1.51
Bud Site -16.06 -24.76 -22.56 -8.97 -0.82 -2.16 -6.61 -8.27 -7.31 2.69 -12.04 -22.34 -21.27 -13.08 2.35
Cell Periphery 5.57 -14.88 -9.15 -14.35 4.92 13.98 2.43 9.01 -4.21 2.33 14.21 -8.68 0.38 -13.78 9.00
Cytoplasm 7.51 30.34 27.04 19.48 -1.79 6.82 28.06 26.17 22.55 -11.69 9.14 41.53 38.26 31.93 -6.15
Cytoplasmic Foci -15.07 8.82 12.29 22.24 5.72 -5.82 -3.69 1.49 6.80 4.16 -14.54 8.26 10.49 22.45 1.84
Eisosomes 11.18 10.09 -1.68 -11.30 -10.30 -6.17 2.28 -1.80 3.00 -3.28 1.00 10.51 -3.02 -3.85 -11.29
Endoplasmic Reticulum -3.32 -21.27 -4.80 -0.63 19.76 4.65 -7.95 -0.05 -4.30 7.92 1.08 -21.83 -2.99 -4.33 21.03
Endosome -11.33 -2.02 -2.28 8.40 -1.84 -5.18 -3.20 -1.15 2.08 0.61 -12.03 -0.63 -2.32 7.10 -2.17
Golgi -9.35 6.84 1.19 7.21 -1.14 -2.78 17.45 8.20 8.79 -0.43 -8.02 18.44 6.30 10.76 -0.94
Lipid Particles -5.56 -5.66 7.96 8.17 11.98 -5.40 -5.55 2.99 7.12 6.14 -7.49 -8.69 8.04 11.19 13.06
Mitochondria -2.30 9.49 0.02 1.16 -1.83 -6.97 12.88 5.38 9.60 -2.82 -6.68 12.39 0.85 4.19 -1.77
Mitotic Spindle 0.46 -1.44 -2.86 -2.89 -2.76 -0.21 -1.10 -2.79 -2.78 -1.40 0.22 -2.14 -3.99 -4.01 -3.71
None 2.21 4.47 5.10 2.50 2.96 0.16 3.76 3.34 2.84 -1.29 2.31 4.55 5.94 3.60 4.00
Nuclear Periphery 0.81 -7.62 1.48 0.25 5.86 1.28 -6.33 -2.61 -3.16 4.14 1.88 -11.64 -0.37 -1.65 8.13
Nuclear Periphery Foci -2.68 -17.02 -4.36 -1.47 14.00 2.22 -6.35 -1.01 -10.98 6.38 1.79 -9.97 -1.48 -10.05 13.58
Nucleolus -0.47 7.37 7.72 3.37 2.13 -0.16 2.15 2.17 17.41 0.17 -0.20 4.31 4.99 5.99 4.36
Nucleus 22.36 4.34 13.48 -12.40 10.64 -8.66 -7.46 -11.10 -4.62 3.36 15.04 -8.22 5.44 -11.96 13.59
Peroxisomes -5.85 7.49 5.22 11.30 -3.19 -10.04 2.90 0.35 5.13 -1.56 -11.09 12.78 3.18 11.11 -3.44
Vacuole -13.69 -18.60 -21.99 -13.27 -13.54 -9.20 -13.58 -27.25 -25.12 1.86 -16.20 -20.60 -34.28 -28.32 -18.47
Vacuole Periphery -5.58 4.51 -2.32 0.25 -2.55 -2.38 11.10 2.57 3.85 -1.90 -6.28 10.75 -1.86 0.16 -2.72
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null
Localization
Cell Percentages cell periphery (37%), cytoplasm (6%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Whi2

Whi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Whi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available