Standard name
Human Ortholog
Description Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0 0 0.05 0.09 0.07 0 0.05 0 0 0.05 0.05 0.08 0.07 0.06 0.06 0.12 0.07 0 0.07 0 0 0 0
Bud 0 0 0.05 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.48 0.49 0.35 0.64 0.35 0.52 0.35 0.26 0.34 0.34 0.28 0.44 0.64 0.59 0.59 0.32 0.39 0.45 0.64 0.51 0.55 0.76 0.78 0.74
Cytoplasm 0.77 0.76 0.91 0.91 0.82 0.78 0.75 0.81 0.66 0.71 0.64 0.57 0.73 0.74 0.82 0.76 0.62 0.59 0.13 0.25 0.23 0.11 0.08 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0.05 0.12 0.21 0.11 0.34 0.26 0.4 0.38 0 0 0 0.05 0.06 0.07 0.06 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 4 4 1 5 6 4 9 2 4 4 6 31 39 35 20 27 12 11 6 1 9 9 13
Bud 10 2 5 2 3 2 6 3 5 1 1 1 7 8 3 2 4 8 2 0 0 0 1 4
Bud Neck 0 0 2 0 0 0 0 0 0 1 0 0 0 0 1 5 3 0 6 7 6 8 11 26
Bud Site 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 106 47 32 14 19 47 47 45 44 37 24 55 264 309 366 104 85 81 172 53 51 223 282 356
Cytoplasm 172 73 83 20 45 71 101 138 86 77 54 71 302 391 511 250 137 106 35 26 21 33 28 44
Endoplasmic Reticulum 1 0 0 0 0 1 3 2 2 1 1 2 4 2 4 18 19 6 10 1 3 3 13 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 0 0 1 2 2
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 5 1 0 2 3 0
Mitochondria 17 3 2 0 3 11 29 18 44 28 34 47 6 3 2 17 13 12 17 3 3 5 5 7
Nucleus 1 0 0 0 0 0 0 1 1 0 1 1 0 0 0 2 0 1 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 1
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 1 0 1 2 5 5 2 0 0 0 0 0 1
Vac/Vac Membrane 0 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0 3 0 2 2 1 1 0 4
Unique Cell Count 223 96 91 22 55 91 135 170 131 108 85 125 412 526 623 327 220 179 269 104 94 295 364 481
Labelled Cell Count 330 130 128 37 75 138 191 216 184 149 120 185 616 753 927 427 299 228 269 104 94 295 364 481


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.2 5.1 5.3 5.5 5.0 4.6 4.9 5.2 5.1 5.1 5.5 6.9 6.3 6.4 5.9 7.1 7.1 6.1 6.5 6.9
Std Deviation (1e-4) 0.6 0.9 1.1 1.1 1.1 0.7 0.8 0.8 0.9 0.9 1.1 1.4 0.9 1.0 1.0 1.1 1.5 1.2 0.9 1.4 1.2
Intensity Change (Log2) – – – 0.06 0.11 -0.02 -0.14 -0.06 0.05 0.01 0.01 0.12 0.44 0.33 0.35 0.23 0.49 0.49 0.26 0.36 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0.7 0 0 0 0 0 0 1.1 1.0 0 0.6 2.1 0.8
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.4 -0.1 2.2 0 0 -0.2 -0.1 -1.0 1.3 5.1 4.2 4.2 -0.6 0.6 1.6
Cytoplasm 0 -1.7 -2.5 -3.1 -2.1 -4.4 -3.5 -4.4 -5.5 -3.6 -3.5 -2.2 -3.1 -5.1 -5.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.6 0 0 5.7 4.7 6.2 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5391 6.6225 6.089 5.7286 5.8933 6.3404 3.573 4.5136 4.2146 3.8972 3.7564 4.1851 5.7488 6.7233 6.021 6.2242 5.4283 6.3104
Actin 0.0378 0.0035 0.0098 0.0071 0.0121 0.0113 0.0484 0.0026 0.0235 0.0268 0.0232 0.0074 0.0578 0.0078 0.0129 0.0076 0.0382 0.0062
Bud 0.0021 0.0004 0.001 0.0005 0.0003 0.0009 0.0014 0.0001 0.0004 0.0003 0.0003 0.0006 0.0012 0.0002 0.0002 0.0001 0.0012 0.0004
Bud Neck 0.0068 0.0013 0.0048 0.0114 0.0048 0.021 0.0099 0.0015 0.0044 0.002 0.0077 0.008 0.0126 0.0018 0.0015 0.0022 0.002 0.0026
Bud Periphery 0.0088 0.0013 0.0102 0.0055 0.0023 0.0077 0.007 0.0007 0.0028 0.0041 0.0034 0.0065 0.0064 0.0011 0.0023 0.0022 0.0036 0.0044
Bud Site 0.0127 0.0258 0.0193 0.0037 0.0036 0.0036 0.0198 0.0295 0.0359 0.0027 0.001 0.0015 0.0158 0.0242 0.0045 0.0015 0.0053 0.0008
Cell Periphery 0.3971 0.3872 0.4633 0.3836 0.4807 0.4942 0.5095 0.5376 0.4889 0.5681 0.5541 0.6601 0.5532 0.5426 0.5823 0.6915 0.5262 0.664
Cytoplasm 0.2916 0.5355 0.4212 0.4897 0.43 0.4061 0.2736 0.3793 0.3897 0.3402 0.281 0.2745 0.2488 0.4036 0.3365 0.2675 0.3057 0.3002
Cytoplasmic Foci 0.0234 0.0009 0.0038 0.001 0.0037 0.004 0.0218 0.0026 0.0035 0.0088 0.0053 0.0034 0.0128 0.0025 0.0078 0.0026 0.0107 0.0002
Eisosomes 0.0064 0.0013 0.001 0.0037 0.0005 0.0021 0.0031 0.0021 0.002 0.0013 0.0025 0.0009 0.0155 0.0007 0.0071 0.0008 0.0008 0.0007
Endoplasmic Reticulum 0.0148 0.0054 0.0076 0.0179 0.0035 0.0078 0.0123 0.0025 0.0033 0.0067 0.0079 0.0044 0.0117 0.0032 0.0075 0.0057 0.0042 0.0075
Endosome 0.0448 0.0008 0.0136 0.0058 0.006 0.0064 0.0207 0.0001 0.0087 0.0066 0.0153 0.0014 0.0054 0.0005 0.0037 0.0024 0.008 0.0013
Golgi 0.0126 0.0006 0.0045 0.007 0.0054 0.0078 0.0133 0.0004 0.0055 0.0107 0.0071 0.002 0.0052 0.0012 0.0027 0.0023 0.0051 0.0011
Lipid Particles 0.0262 0.0011 0.0021 0.007 0.0082 0.0013 0.0166 0.0014 0.0017 0.0055 0.0091 0.0011 0.0125 0.0014 0.0065 0.0076 0.0069 0.0006
Mitochondria 0.0274 0.0096 0.0139 0.0444 0.0045 0.0162 0.0037 0.0001 0.0013 0.0025 0.03 0.0013 0.0033 0.0001 0.0024 0.0008 0.0012 0.0012
None 0.02 0.0221 0.0101 0.0019 0.028 0.005 0.0142 0.0254 0.0239 0.0046 0.0331 0.0245 0.0036 0.0014 0.0089 0.0008 0.0112 0.0012
Nuclear Periphery 0.0094 0.0003 0.0038 0.0013 0.0003 0.0002 0.0036 0.0002 0.0004 0.0007 0.0053 0.0002 0.002 0.0002 0.0012 0.0003 0.0083 0.002
Nucleolus 0.0033 0.0001 0.0002 0.0002 0.0001 0.0001 0.0014 0.0001 0.0001 0.0001 0.0003 0.0001 0.0009 0 0.0003 0.0001 0.0033 0.0002
Nucleus 0.0236 0.0003 0.0008 0.0004 0.0001 0.0003 0.0028 0.0001 0.0002 0.0001 0.0009 0.0002 0.0046 0.0001 0.0003 0.0001 0.0143 0.0029
Peroxisomes 0.0065 0 0.0016 0.0003 0.0041 0.0003 0.008 0.0128 0.002 0.0056 0.0045 0.0003 0.0163 0.0066 0.0046 0.0021 0.0074 0
Punctate Nuclear 0.0061 0.0001 0.0006 0.0001 0.0003 0.0001 0.0036 0.0001 0.0001 0.0003 0.0063 0.0001 0.0053 0.0002 0.0051 0.0005 0.0345 0.0003
Vacuole 0.0159 0.0023 0.0063 0.0061 0.0014 0.0036 0.005 0.0007 0.0013 0.0022 0.0014 0.0016 0.0046 0.0008 0.0016 0.0014 0.0016 0.002
Vacuole Periphery 0.0027 0.0001 0.0006 0.0013 0.0002 0.0001 0.0005 0 0.0002 0.0001 0.0004 0 0.0004 0 0.0001 0.0001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.2305 29.7061 33.5657 35.3846 29.376 27.1736 36.3501 35.2458 31.1444 36.2575
Translational Efficiency 0.8765 0.9767 0.785 0.7889 0.7778 0.8998 0.7396 0.6061 0.8885 0.7274

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null
Localization
Cell Percentages cell periphery (37%), cytoplasm (6%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Whi2

Whi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Whi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available