Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 2 | 9 | 3 | 8 | 3 | – | 3 | 1 | 2 | 2 | 1 | 8 | 13 | 0 | 12 | 9 | 4 | 14 | 8 | 8 | 5 | 15 | 10 |
Bud | 7 | 2 | 19 | 4 | 3 | 10 | – | 23 | 9 | 15 | 24 | 23 | 0 | 2 | 0 | 3 | 6 | 2 | 3 | 1 | 3 | 2 | 8 | 4 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 4 | 2 | 5 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 16 | 13 | 25 | 12 | 24 | – | 30 | 19 | 19 | 16 | 14 | 37 | 31 | 53 | 16 | 6 | 26 | 5 | 5 | 5 | 2 | 4 | 11 |
Cytoplasm | 177 | 70 | 98 | 52 | 123 | 226 | – | 324 | 164 | 242 | 162 | 264 | 102 | 93 | 132 | 41 | 56 | 45 | 97 | 23 | 35 | 28 | 45 | 63 |
Endoplasmic Reticulum | 160 | 199 | 68 | 83 | 93 | 70 | – | 47 | 61 | 66 | 37 | 42 | 255 | 158 | 213 | 0 | 6 | 5 | 350 | 237 | 207 | 138 | 275 | 224 |
Endosome | 6 | 0 | 0 | 0 | 2 | 6 | – | 5 | 1 | 3 | 0 | 3 | 1 | 4 | 1 | 3 | 4 | 3 | 2 | 0 | 1 | 1 | 5 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 3 |
Mitochondria | 24 | 3 | 68 | 95 | 50 | 202 | – | 338 | 245 | 289 | 204 | 365 | 4 | 2 | 2 | 4 | 13 | 5 | 15 | 6 | 30 | 15 | 19 | 16 |
Nucleus | 8 | 2 | 0 | 1 | 0 | 1 | – | 15 | 2 | 2 | 4 | 6 | 0 | 1 | 1 | 3 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 3 | 3 | 0 | 3 | 1 | 1 | – | 13 | 4 | 4 | 7 | 8 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 62 | 8 | 14 | 5 | 16 | 32 | – | 112 | 33 | 45 | 25 | 43 | 5 | 5 | 18 | 32 | 38 | 52 | 20 | 4 | 9 | 6 | 16 | 24 |
Unique Cell Count | 407 | 260 | 234 | 190 | 258 | 445 | 735 | 440 | 553 | 384 | 627 | 360 | 268 | 365 | 96 | 116 | 114 | 522 | 294 | 312 | 208 | 405 | 373 | |
Labelled Cell Count | 464 | 305 | 291 | 271 | 308 | 576 | 913 | 539 | 691 | 482 | 772 | 413 | 314 | 423 | 116 | 140 | 146 | 522 | 294 | 312 | 208 | 405 | 373 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 8.4 | 4.8 | 5.6 | 6.0 | 4.8 | – | 4.8 | 4.1 | 4.5 | 4.6 | 4.4 | 7.4 | 7.4 | 7.2 | 10.3 | 8.7 | 10.1 | 6.8 | 7.3 | 7.4 |
Std Deviation (1e-4) | 1.1 | 1.2 | 0.9 | 1.3 | 1.6 | 1.1 | – | 1.2 | 1.0 | 0.9 | 1.1 | 0.9 | 1.2 | 1.1 | 1.0 | 1.8 | 1.9 | 2.0 | 1.1 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.22 | 0.33 | -0.0 | – | -0.0 | -0.22 | -0.08 | -0.07 | -0.12 | 0.63 | 0.63 | 0.59 | 1.11 | 0.86 | 1.07 | 0.51 | 0.6 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -0.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.2 | 0.5 | 0 | 2.9 | 1.6 | 0 |
Bud | -2.7 | -3.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | -5.5 | -4.1 | -5.5 | -1.7 | -1.0 | -2.3 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.7 | -0.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.4 | 3.4 | 3.2 | -0.1 | 4.8 |
Cytoplasm | -3.1 | 1.3 | 2.2 | – | 0.6 | -1.2 | 0.5 | 0.1 | 0.1 | -3.4 | -1.7 | -1.4 | 0.1 | 1.1 | -0.4 |
Endoplasmic Reticulum | 3.1 | 1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 10.0 | 6.7 | 7.0 | -5.9 | -5.2 | -5.3 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | -2.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | -10.2 | -9.1 | -10.6 | -5.0 | -3.7 | -5.3 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.7 | 0.1 | 0.6 | – | 3.7 | 0.7 | 1.0 | 0.3 | 0.5 | -3.1 | -2.4 | -0.6 | 6.5 | 6.6 | 8.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.888 | 4.0042 | 2.9824 | 2.3285 | 2.4406 | 3.1235 | 3.0268 | 3.8956 | 3.5124 | 3.2445 | 2.6836 | 3.3868 | 4.2937 | 5.6413 | 5.0127 | 4.6768 | 4.6863 | 5.1953 |
Actin | 0.0272 | 0.0016 | 0.0144 | 0.0346 | 0.0964 | 0.0091 | 0.0656 | 0.0014 | 0.031 | 0.0029 | 0.0209 | 0.0015 | 0.045 | 0.0011 | 0.0059 | 0.0041 | 0.0026 | 0.0064 |
Bud | 0.0004 | 0.0001 | 0.0023 | 0.0032 | 0.0011 | 0.0002 | 0.0012 | 0.0015 | 0.0042 | 0.0004 | 0.0013 | 0.0003 | 0.0006 | 0.0046 | 0.0052 | 0.0001 | 0.0019 | 0.0001 |
Bud Neck | 0.0004 | 0.0003 | 0.0022 | 0.0011 | 0.0009 | 0.0097 | 0.0085 | 0.0004 | 0.0081 | 0.0019 | 0.0021 | 0.0029 | 0.0017 | 0.0003 | 0.0006 | 0.0006 | 0.0008 | 0.002 |
Bud Periphery | 0.0007 | 0.0002 | 0.0094 | 0.005 | 0.0017 | 0.0007 | 0.0024 | 0.0008 | 0.0042 | 0.0008 | 0.0029 | 0.0003 | 0.0007 | 0.001 | 0.0026 | 0.0003 | 0.0037 | 0.0001 |
Bud Site | 0.0011 | 0.0015 | 0.006 | 0.0112 | 0.0016 | 0.0001 | 0.018 | 0.0144 | 0.0074 | 0.0012 | 0.0027 | 0.0002 | 0.0032 | 0.0068 | 0.0077 | 0.0006 | 0.0031 | 0.0002 |
Cell Periphery | 0.0017 | 0.0032 | 0.0017 | 0.0005 | 0.0023 | 0.0013 | 0.0057 | 0.0034 | 0.0023 | 0.0007 | 0.0014 | 0.0009 | 0.0022 | 0.0034 | 0.0035 | 0.0011 | 0.0014 | 0.0007 |
Cytoplasm | 0.2062 | 0.3039 | 0.2266 | 0.1129 | 0.1994 | 0.2997 | 0.2054 | 0.2028 | 0.2238 | 0.2549 | 0.216 | 0.1972 | 0.1925 | 0.3178 | 0.2173 | 0.117 | 0.3954 | 0.2474 |
Cytoplasmic Foci | 0.0196 | 0.004 | 0.0166 | 0.0116 | 0.0282 | 0.0034 | 0.0311 | 0.0086 | 0.0116 | 0.0065 | 0.0224 | 0.01 | 0.017 | 0.0061 | 0.0099 | 0.0065 | 0.0087 | 0.0046 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.549 | 0.6287 | 0.6141 | 0.6252 | 0.5091 | 0.6103 | 0.4478 | 0.6633 | 0.6034 | 0.6385 | 0.4152 | 0.7324 | 0.5814 | 0.5812 | 0.6734 | 0.7529 | 0.4247 | 0.6853 |
Endosome | 0.0326 | 0.0077 | 0.0268 | 0.0603 | 0.0456 | 0.011 | 0.0635 | 0.0252 | 0.0271 | 0.0238 | 0.0957 | 0.0088 | 0.0187 | 0.0101 | 0.0138 | 0.0154 | 0.0255 | 0.0114 |
Golgi | 0.0103 | 0.001 | 0.0141 | 0.0358 | 0.0255 | 0.0026 | 0.0229 | 0.0031 | 0.0093 | 0.0078 | 0.0299 | 0.0014 | 0.0122 | 0.001 | 0.0051 | 0.0089 | 0.0142 | 0.0016 |
Lipid Particles | 0.0215 | 0.0062 | 0.0093 | 0.0169 | 0.0122 | 0.0025 | 0.0233 | 0.018 | 0.0109 | 0.0084 | 0.0324 | 0.0084 | 0.018 | 0.0167 | 0.0057 | 0.0336 | 0.0028 | 0.0024 |
Mitochondria | 0.0038 | 0.0002 | 0.0099 | 0.0295 | 0.0091 | 0.0011 | 0.0109 | 0.0009 | 0.0139 | 0.0034 | 0.0332 | 0.0006 | 0.0131 | 0.0004 | 0.0107 | 0.0227 | 0.0395 | 0.0007 |
None | 0.072 | 0.0175 | 0.0107 | 0.0081 | 0.0052 | 0.0197 | 0.0247 | 0.0292 | 0.0132 | 0.0022 | 0.0509 | 0.0162 | 0.0404 | 0.0288 | 0.0168 | 0.0054 | 0.0537 | 0.015 |
Nuclear Periphery | 0.0125 | 0.0144 | 0.0161 | 0.0175 | 0.0113 | 0.0193 | 0.0136 | 0.0128 | 0.0126 | 0.0274 | 0.0218 | 0.0101 | 0.0153 | 0.0087 | 0.009 | 0.016 | 0.0096 | 0.0105 |
Nucleolus | 0.0013 | 0.0001 | 0.0003 | 0.0018 | 0.0001 | 0.0001 | 0.0012 | 0.0003 | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0057 | 0.0009 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0035 | 0.0044 | 0.0066 | 0.0045 | 0.0014 | 0.0037 | 0.0063 | 0.0033 | 0.0036 | 0.0085 | 0.0089 | 0.0031 | 0.0088 | 0.0036 | 0.0017 | 0.0018 | 0.0037 | 0.0063 |
Peroxisomes | 0.0213 | 0 | 0.0054 | 0.0082 | 0.042 | 0.0001 | 0.0164 | 0.0002 | 0.0024 | 0.0013 | 0.0264 | 0.0003 | 0.0105 | 0.0001 | 0.0018 | 0.0048 | 0.0007 | 0.0002 |
Punctate Nuclear | 0.0059 | 0.0006 | 0.0013 | 0.0034 | 0.002 | 0.0011 | 0.0188 | 0.0025 | 0.0023 | 0.0016 | 0.0045 | 0.002 | 0.0083 | 0.0009 | 0.0005 | 0.0023 | 0.0008 | 0.0008 |
Vacuole | 0.0065 | 0.0039 | 0.0046 | 0.0059 | 0.0027 | 0.0032 | 0.0095 | 0.007 | 0.0066 | 0.0062 | 0.007 | 0.0028 | 0.0036 | 0.0063 | 0.0069 | 0.0047 | 0.0056 | 0.0038 |
Vacuole Periphery | 0.0023 | 0.0004 | 0.0014 | 0.0023 | 0.0018 | 0.0009 | 0.0019 | 0.0008 | 0.001 | 0.0013 | 0.0033 | 0.0003 | 0.0008 | 0.0003 | 0.0011 | 0.0011 | 0.0015 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.6229 | 8.3238 | 7.7208 | 10.5614 | 18.109 | 18.6126 | 14.2024 | 15.6211 | 8.8954 | 16.8314 |
Translational Efficiency | 0.4076 | 0.8157 | 0.7586 | 0.4078 | 0.3579 | 0.8081 | 0.715 | 0.4408 | 0.8213 | 0.6004 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1930 | 1423 | 991 | 1341 | 1323 | 87 | 2211 | 1366 | 3253 | 1510 | 3202 | 2707 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 819.37 | 957.83 | 1217.49 | 1068.94 | 804.93 | 1009.38 | 1083.11 | 998.12 | 813.50 | 960.80 | 1124.70 | 1033.20 |
Standard Deviation | 99.07 | 148.73 | 163.45 | 133.93 | 111.25 | 149.41 | 127.38 | 148.79 | 104.44 | 149.25 | 152.74 | 145.98 |
Intensity Change Log 2 | — | 0.225255 | 0.571323 | 0.383594 | — | 0.326534 | 0.428245 | 0.310350 | — | 0.276333 | 0.502192 | 0.347762 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000563 | 0.002343 | 0.001807 | 0.023125 | 0.000846 | 0.001718 | 0.003679 | 0.013272 | 0.000678 | 0.002307 | 0.003100 | 0.018153 |
Bud Neck | 0.003908 | 0.003076 | 0.004402 | 0.017776 | 0.004235 | 0.002507 | 0.009684 | 0.022739 | 0.004041 | 0.003043 | 0.008049 | 0.020280 |
Bud Site | 0.006212 | 0.008599 | 0.015755 | 0.071911 | 0.007315 | 0.011822 | 0.018488 | 0.047389 | 0.006660 | 0.008784 | 0.017642 | 0.059537 |
Cell Periphery | 0.001333 | 0.003012 | 0.003156 | 0.009514 | 0.001406 | 0.012372 | 0.004165 | 0.009011 | 0.001363 | 0.003552 | 0.003853 | 0.009260 |
Cytoplasm | 0.272594* | 0.115200* | 0.143754* | 0.209610* | 0.233267* | 0.125523* | 0.222327* | 0.268903* | 0.256600* | 0.115795* | 0.198009* | 0.239530* |
Cytoplasmic Foci | 0.061569 | 0.023988 | 0.023934 | 0.052730 | 0.078561 | 0.037124 | 0.010763 | 0.070304 | 0.068480 | 0.024745 | 0.014839 | 0.061598 |
Eisosomes | 0.000835 | 0.001266 | 0.000190 | 0.004628 | 0.001134 | 0.003258 | 0.001256 | 0.005893 | 0.000957 | 0.001381 | 0.000926 | 0.005266 |
Endoplasmic Reticulum | 0.480031* | 0.762916* | 0.623369* | 0.264121* | 0.474487* | 0.680601* | 0.525689* | 0.220281* | 0.477776* | 0.758173* | 0.555921* | 0.241999* |
Endosome | 0.006788 | 0.006121 | 0.014445 | 0.022122 | 0.008743 | 0.007537 | 0.006972 | 0.018567 | 0.007583 | 0.006203 | 0.009285 | 0.020328 |
Golgi | 0.020172 | 0.012101 | 0.001870 | 0.008881 | 0.015586 | 0.029429 | 0.002298 | 0.009500 | 0.018307 | 0.013100 | 0.002166 | 0.009194 |
Lipid Particles | 0.056977 | 0.019870 | 0.023090 | 0.057312 | 0.065600 | 0.030131 | 0.019990 | 0.051435 | 0.060484 | 0.020461 | 0.020949 | 0.054346 |
Mitochondria | 0.008744 | 0.006360 | 0.000518 | 0.004510 | 0.011444 | 0.014092 | 0.001526 | 0.004179 | 0.009842 | 0.006805 | 0.001214 | 0.004343 |
Mitotic Spindle | 0.000294 | 0.000753 | 0.001016 | 0.010993 | 0.000156 | 0.001375 | 0.001490 | 0.004640 | 0.000238 | 0.000789 | 0.001344 | 0.007787 |
None | 0.008131 | 0.004881 | 0.001765 | 0.000385 | 0.003501 | 0.000153 | 0.001463 | 0.001183 | 0.006248 | 0.004609 | 0.001556 | 0.000787 |
Nuclear Periphery | 0.002530 | 0.000952 | 0.010127 | 0.001688 | 0.003970 | 0.001408 | 0.004400 | 0.002136 | 0.003116 | 0.000978 | 0.006173 | 0.001914 |
Nuclear Periphery Foci | 0.005701 | 0.004967 | 0.061244 | 0.024305 | 0.008079 | 0.007793 | 0.021610 | 0.015999 | 0.006668 | 0.005130 | 0.033876 | 0.020114 |
Nucleolus | 0.000398 | 0.000397 | 0.000193 | 0.000232 | 0.000455 | 0.000031 | 0.000139 | 0.000582 | 0.000421 | 0.000376 | 0.000156 | 0.000408 |
Nucleus | 0.044612 | 0.009524 | 0.025097 | 0.038596 | 0.053611 | 0.004406 | 0.087258 | 0.048076 | 0.048272 | 0.009229 | 0.068020 | 0.043380 |
Peroxisomes | 0.003252 | 0.003265 | 0.000607 | 0.004204 | 0.004799 | 0.014067 | 0.000728 | 0.005751 | 0.003881 | 0.003888 | 0.000691 | 0.004985 |
Vacuole | 0.013527 | 0.008893 | 0.043183 | 0.169376 | 0.020791 | 0.013914 | 0.055586 | 0.178189 | 0.016481 | 0.009183 | 0.051748 | 0.173823 |
Vacuole Periphery | 0.001828 | 0.001514 | 0.000476 | 0.003981 | 0.002016 | 0.000739 | 0.000488 | 0.001972 | 0.001904 | 0.001469 | 0.000484 | 0.002967 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.57 | -4.14 | -11.01 | -9.80 | -10.22 | -1.55 | -6.55 | -10.59 | -8.87 | -7.29 | -3.46 | -7.82 | -14.84 | -12.40 | -12.01 |
Bud Neck | 0.99 | -0.89 | -10.59 | -10.76 | -9.93 | 1.47 | -5.96 | -10.33 | -10.20 | -6.57 | 1.36 | -6.23 | -14.62 | -14.21 | -9.81 |
Bud Site | -1.73 | -3.96 | -15.71 | -14.92 | -11.65 | -0.59 | -5.72 | -11.40 | -4.23 | -7.44 | -1.59 | -7.64 | -19.44 | -17.81 | -13.65 |
Cell Periphery | -3.37 | -3.42 | -7.99 | -5.49 | -5.25 | -2.23 | -7.69 | -8.38 | 0.81 | -4.81 | -4.00 | -8.66 | -11.59 | -6.11 | -6.98 |
Cytoplasm | 22.93 | 14.63 | 8.03 | -12.87 | -7.07 | 5.00 | 1.22 | -3.74 | -6.53 | -5.41 | 23.89 | 10.21 | 3.10 | -19.93 | -6.61 |
Cytoplasmic Foci | 12.75 | 11.78 | 2.45 | -10.19 | -9.37 | 3.89 | 19.78 | 1.41 | -3.33 | -20.20 | 16.50 | 25.28 | 2.02 | -14.72 | -23.37 |
Eisosomes | -1.51 | 9.24 | -7.65 | -5.69 | -9.20 | -1.43 | -0.72 | -7.10 | -1.39 | -7.02 | -1.49 | 0.29 | -10.34 | -7.52 | -10.45 |
Endoplasmic Reticulum | -24.87* | -9.65 | 20.36 | 44.70* | 26.16 | -4.95 | -2.92 | 20.87 | 11.55 | 27.63* | -27.72 | -7.31 | 29.61 | 54.56* | 36.84* |
Endosome | 1.13 | -8.25 | -10.06 | -10.34 | -3.92 | 0.54 | 2.30 | -8.05 | -4.44 | -9.26 | 2.60 | -3.57 | -13.12 | -13.73 | -10.40 |
Golgi | 6.02 | 17.64 | 9.47 | 3.56 | -7.36 | -2.10 | 14.22 | 5.49 | 3.15 | -7.48 | 4.51 | 22.27 | 10.86 | 4.45 | -10.34 |
Lipid Particles | 14.96 | 11.87 | 0.29 | -11.51 | -9.49 | 5.70 | 14.40 | 4.03 | -3.31 | -10.85 | 18.56 | 19.79 | 2.93 | -14.21 | -14.88 |
Mitochondria | 2.37 | 11.10 | 4.29 | 2.20 | -4.46 | -0.25 | 6.20 | 4.28 | 0.98 | -2.96 | 2.58 | 10.94 | 6.12 | 2.76 | -4.88 |
Mitotic Spindle | -1.50 | -2.97 | -6.26 | -5.88 | -5.71 | -1.05 | -3.42 | -4.48 | -2.47 | -3.03 | -1.92 | -3.92 | -7.67 | -6.84 | -6.17 |
None | 2.26 | 5.22 | 7.59 | 4.42 | 2.03 | 4.12 | 2.49 | 2.75 | -3.22 | 0.88 | 1.39 | 6.45 | 7.77 | 3.97 | 2.72 |
Nuclear Periphery | 5.96 | -7.60 | 2.65 | -2.72 | 8.39 | 4.47 | -0.79 | 3.40 | -1.32 | 4.01 | 8.55 | -6.63 | 3.97 | -3.56 | 8.96 |
Nuclear Periphery Foci | 1.14 | -15.88 | -10.78 | -11.07 | 9.52 | 0.15 | -11.87 | -6.61 | -4.12 | 3.52 | 2.59 | -20.19 | -12.87 | -13.71 | 8.18 |
Nucleolus | -0.02 | 3.35 | 3.26 | 0.95 | -0.58 | 5.74 | 4.24 | -0.74 | -3.35 | -2.69 | 0.25 | 6.31 | 0.10 | -0.18 | -2.90 |
Nucleus | 17.61 | 6.93 | 1.65 | -14.77 | -5.22 | 17.25 | -9.01 | 1.46 | -18.15 | 11.12 | 22.59 | -8.04 | 1.81 | -21.01 | 9.61 |
Peroxisomes | -0.04 | 10.39 | -1.90 | -1.45 | -9.42 | -1.48 | 10.04 | -1.25 | 1.35 | -10.03 | -0.01 | 14.66 | -2.56 | -1.55 | -13.67 |
Vacuole | 3.72 | -11.12 | -29.61 | -30.22 | -22.52 | 1.12 | -15.59 | -27.35 | -21.37 | -19.84 | 6.24 | -20.41 | -40.43 | -41.52 | -29.19 |
Vacuole Periphery | 0.90 | 4.53 | -2.15 | -2.65 | -4.11 | 4.29 | 6.29 | 0.37 | -2.09 | -2.73 | 1.68 | 6.67 | -1.61 | -2.55 | -4.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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