Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 9 | 9 | 15 | 2 | 3 | 1 | 0 | 0 | 5 | 7 | 32 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 8 | 26 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 1 | 0 | 5 | 10 | 19 | 20 | 25 | 35 | 24 | 15 | 32 | 34 | 56 | 71 | 54 | 46 | 17 | 2 | 1 | 0 | 2 | 3 | 3 |
Endoplasmic Reticulum | 9 | 2 | 1 | 3 | 3 | 14 | 4 | 18 | 8 | 10 | 1 | 10 | 41 | 56 | 39 | 9 | 8 | 2 | 10 | 5 | 5 | 17 | 45 | 42 |
Endosome | 0 | 0 | 0 | 2 | 0 | 7 | 3 | 11 | 3 | 1 | 1 | 1 | 1 | 10 | 24 | 4 | 0 | 0 | 4 | 1 | 3 | 11 | 43 | 100 |
Golgi | 2 | 0 | 0 | 1 | 1 | 4 | 1 | 3 | 1 | 0 | 1 | 2 | 42 | 62 | 86 | 87 | 96 | 68 | 1 | 0 | 0 | 7 | 49 | 55 |
Mitochondria | 5 | 0 | 2 | 8 | 9 | 27 | 46 | 42 | 50 | 72 | 34 | 73 | 0 | 0 | 1 | 17 | 9 | 5 | 0 | 0 | 1 | 1 | 4 | 10 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 5 | 1 | 4 | 8 | 8 | 3 | 4 | 2 | 1 | 2 | 1 | 1 | 0 | 3 | 3 | 0 | 0 | 2 | 3 |
Nuclear Periphery | 28 | 4 | 5 | 30 | 29 | 36 | 53 | 67 | 42 | 70 | 17 | 47 | 43 | 53 | 27 | 7 | 3 | 3 | 147 | 33 | 53 | 126 | 273 | 281 |
Nucleolus | 15 | 2 | 1 | 9 | 7 | 10 | 15 | 21 | 13 | 10 | 6 | 13 | 12 | 28 | 25 | 7 | 5 | 3 | 2 | 1 | 0 | 1 | 4 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 5 | 5 |
Vac/Vac Membrane | 113 | 12 | 10 | 34 | 57 | 31 | 59 | 75 | 33 | 48 | 9 | 23 | 165 | 241 | 357 | 137 | 146 | 95 | 9 | 12 | 0 | 7 | 25 | 26 |
Unique Cell Count | 149 | 16 | 16 | 65 | 92 | 112 | 145 | 206 | 147 | 186 | 73 | 151 | 255 | 414 | 526 | 261 | 247 | 149 | 189 | 66 | 68 | 193 | 482 | 605 |
Labelled Cell Count | 178 | 22 | 19 | 94 | 117 | 154 | 203 | 269 | 195 | 246 | 88 | 207 | 343 | 517 | 644 | 339 | 317 | 196 | 189 | 66 | 68 | 193 | 482 | 605 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.7 | 18.4 | 13.0 | 12.6 | 12.0 | 10.4 | 9.6 | 9.8 | 9.2 | 9.2 | 8.0 | 8.3 | 21.3 | 20.6 | 20.4 | 22.1 | 25.3 | 30.5 | 20.0 | 19.8 | 20.7 |
Std Deviation (1e-4) | 3.9 | 4.7 | 2.9 | 3.4 | 2.9 | 2.7 | 2.5 | 2.4 | 2.3 | 2.1 | 2.1 | 2.1 | 5.0 | 4.5 | 4.7 | 6.5 | 7.5 | 7.5 | 4.5 | 4.1 | 4.3 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.11 | -0.32 | -0.43 | -0.4 | -0.49 | -0.51 | -0.7 | -0.65 | 0.71 | 0.66 | 0.65 | 0.76 | 0.96 | 1.23 | 0.62 | 0.61 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.1 | 3.5 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.1 | 2.5 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1.1 | 0 | 0.1 | 0.4 | 0.1 | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.7 | 0 | -2.8 | -1.7 | -2.1 | 0 | 0 | 0 | 0 | 0.2 | -0.3 | 0.5 | -0.8 | -0.3 | 0.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.5665 | 16.8899 | 16.7192 | 15.7612 | 17.1848 | 16.0576 | 12.9463 | 13.1523 | 12.7349 | 11.8698 | 10.6437 | 11.9062 | 14.8184 | 17.3042 | 15.2752 | 15.1367 | 14.3482 | 14.9917 |
Actin | 0.0139 | 0.0034 | 0.0093 | 0.0022 | 0.0002 | 0.0004 | 0.0122 | 0.0347 | 0.0046 | 0.0175 | 0.0049 | 0.0035 | 0.0358 | 0.0011 | 0.0088 | 0.0265 | 0.0006 | 0.0103 |
Bud | 0.0001 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0069 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 |
Bud Neck | 0.0004 | 0.0003 | 0.0005 | 0.0006 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0011 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0011 | 0 | 0.0002 |
Bud Periphery | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0005 | 0.0006 | 0.0008 | 0.0074 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0015 | 0 | 0.0002 |
Bud Site | 0.0002 | 0.0007 | 0.0021 | 0 | 0 | 0 | 0.0035 | 0.0018 | 0.0006 | 0.0004 | 0.042 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0006 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 |
Cytoplasm | 0.0055 | 0.0384 | 0.009 | 0.0044 | 0.0074 | 0.0031 | 0.0112 | 0.0132 | 0.0028 | 0.0013 | 0.0262 | 0.0022 | 0.0057 | 0.0053 | 0.0009 | 0.0071 | 0.0002 | 0.0004 |
Cytoplasmic Foci | 0.0057 | 0.0004 | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0227 | 0.0028 | 0.0095 | 0.0013 | 0.0114 | 0.0032 | 0.0187 | 0.0004 | 0.0076 | 0.0168 | 0.0001 | 0.0023 |
Eisosomes | 0.0002 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0148 | 0.0228 | 0.0202 | 0.0246 | 0.0033 | 0.0033 | 0.0096 | 0.0314 | 0.0194 | 0.0284 | 0.0043 | 0.0083 | 0.015 | 0.0395 | 0.0262 | 0.0766 | 0.0113 | 0.0054 |
Endosome | 0.0198 | 0.0027 | 0.0042 | 0.0035 | 0.0014 | 0.0013 | 0.0273 | 0.008 | 0.0308 | 0.0113 | 0.0349 | 0.0184 | 0.0219 | 0.0083 | 0.0064 | 0.0058 | 0.0043 | 0.0041 |
Golgi | 0.0024 | 0.0004 | 0.0008 | 0.0007 | 0.0005 | 0.0001 | 0.0063 | 0.0049 | 0.0097 | 0.0043 | 0.0051 | 0.0071 | 0.0033 | 0.001 | 0.0018 | 0.0026 | 0.0005 | 0.0028 |
Lipid Particles | 0.0034 | 0.0007 | 0.0012 | 0.0014 | 0.0022 | 0.0004 | 0.0155 | 0.011 | 0.017 | 0.0054 | 0.0014 | 0.0181 | 0.0195 | 0.0058 | 0.0044 | 0.0027 | 0.0009 | 0.0063 |
Mitochondria | 0.0025 | 0.0025 | 0.0007 | 0.0007 | 0.0002 | 0.0003 | 0.0037 | 0.0026 | 0.003 | 0.0055 | 0.044 | 0.0181 | 0.0019 | 0.0011 | 0.0011 | 0.0031 | 0.0006 | 0.0018 |
None | 0.0029 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0175 | 0.0059 | 0.0232 | 0.0005 | 0.0005 | 0.0005 | 0.0046 | 0.0002 | 0.0008 | 0.0013 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.8524 | 0.8084 | 0.8742 | 0.8442 | 0.8934 | 0.9407 | 0.8132 | 0.8128 | 0.812 | 0.8513 | 0.7703 | 0.8917 | 0.8155 | 0.8609 | 0.8851 | 0.7436 | 0.9537 | 0.9317 |
Nucleolus | 0.0007 | 0.0043 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.001 | 0.001 | 0.0009 | 0.0002 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 |
Nucleus | 0.0343 | 0.0889 | 0.0531 | 0.0952 | 0.0727 | 0.0362 | 0.0114 | 0.0101 | 0.0136 | 0.0201 | 0.0036 | 0.0055 | 0.0089 | 0.0391 | 0.0151 | 0.0539 | 0.0074 | 0.0128 |
Peroxisomes | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0066 | 0.0004 | 0.0047 | 0.0002 | 0.001 | 0.0005 | 0.0026 | 0 | 0.0017 | 0.0114 | 0 | 0.0032 |
Punctate Nuclear | 0.0137 | 0.0024 | 0.0021 | 0.002 | 0.0014 | 0.0014 | 0.0022 | 0.0021 | 0.0056 | 0.007 | 0.0008 | 0.0008 | 0.01 | 0.003 | 0.0059 | 0.005 | 0.0009 | 0.0016 |
Vacuole | 0.003 | 0.0045 | 0.0018 | 0.002 | 0.0007 | 0.0013 | 0.0022 | 0.0039 | 0.0037 | 0.0043 | 0.0092 | 0.0015 | 0.0044 | 0.006 | 0.0029 | 0.0031 | 0.0009 | 0.0011 |
Vacuole Periphery | 0.0235 | 0.0161 | 0.018 | 0.0177 | 0.0159 | 0.0107 | 0.0333 | 0.0522 | 0.0373 | 0.0384 | 0.0231 | 0.0189 | 0.0299 | 0.0272 | 0.0299 | 0.036 | 0.0184 | 0.0149 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 111.5588 | 106.1937 | 78.2952 | 104.9463 | 109.5914 | 84.479 | 96.0157 | 87.3346 | 80.8701 | 103.2363 |
Translational Efficiency | 1.87 | 2.0253 | 2.3243 | 1.5438 | 1.6927 | 2.4511 | 1.7615 | 1.7943 | 2.0011 | 1.7059 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1844 | 1094 | 108 | 1559 | 1211 | 1203 | 174 | 146 | 3055 | 2297 | 282 | 1705 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1399.16 | 1546.76 | 2068.64 | 2345.65 | 1544.84 | 1599.95 | 2493.52 | 2520.98 | 1456.91 | 1574.62 | 2330.80 | 2360.66 |
Standard Deviation | 350.54 | 378.82 | 390.86 | 425.34 | 280.30 | 305.16 | 352.71 | 502.35 | 332.25 | 343.26 | 421.81 | 435.24 |
Intensity Change Log 2 | — | 0.144688 | 0.564122 | 0.745427 | — | 0.050569 | 0.690726 | 0.706527 | — | 0.096067 | 0.631939 | 0.725145 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001007 | 0.000305 | 0.000191 | 0.000096 | 0.000185 | 0.000170 | 0.000063 | 0.000159 | 0.000681 | 0.000234 | 0.000112 | 0.000101 |
Bud Neck | 0.001899 | 0.001848 | 0.010721 | 0.003778 | 0.001947 | 0.001440 | 0.003736 | 0.011215 | 0.001918 | 0.001634 | 0.006411 | 0.004415 |
Bud Site | 0.000529 | 0.000504 | 0.000520 | 0.001272 | 0.000438 | 0.000678 | 0.000105 | 0.003629 | 0.000493 | 0.000595 | 0.000264 | 0.001474 |
Cell Periphery | 0.000254 | 0.000176 | 0.000136 | 0.000231 | 0.000129 | 0.000064 | 0.000067 | 0.000367 | 0.000204 | 0.000117 | 0.000094 | 0.000242 |
Cytoplasm | 0.011335 | 0.018475 | 0.000623 | 0.010818 | 0.010052 | 0.008305 | 0.000301 | 0.003076 | 0.010826 | 0.013148 | 0.000424 | 0.010155 |
Cytoplasmic Foci | 0.001789 | 0.001495 | 0.001084 | 0.002655 | 0.001870 | 0.001782 | 0.000350 | 0.003000 | 0.001821 | 0.001645 | 0.000631 | 0.002684 |
Eisosomes | 0.000006 | 0.000002 | 0.000004 | 0.000002 | 0.000002 | 0.000002 | 0.000001 | 0.000003 | 0.000004 | 0.000002 | 0.000002 | 0.000002 |
Endoplasmic Reticulum | 0.011262 | 0.006002 | 0.002202 | 0.007201 | 0.007047 | 0.004414 | 0.001952 | 0.003670 | 0.009591 | 0.005170 | 0.002048 | 0.006898 |
Endosome | 0.006403 | 0.005283 | 0.002559 | 0.009432 | 0.006167 | 0.003536 | 0.001471 | 0.011655 | 0.006310 | 0.004368 | 0.001888 | 0.009622 |
Golgi | 0.002854 | 0.003196 | 0.000929 | 0.011586 | 0.002291 | 0.001684 | 0.000800 | 0.011548 | 0.002631 | 0.002404 | 0.000849 | 0.011583 |
Lipid Particles | 0.012971 | 0.002789 | 0.002319 | 0.013462 | 0.005751 | 0.001431 | 0.001503 | 0.013998 | 0.010109 | 0.002078 | 0.001815 | 0.013508 |
Mitochondria | 0.013555 | 0.002624 | 0.000803 | 0.000690 | 0.001157 | 0.002115 | 0.000396 | 0.000161 | 0.008640 | 0.002357 | 0.000552 | 0.000644 |
Mitotic Spindle | 0.005764 | 0.016458 | 0.029741 | 0.090825 | 0.010508 | 0.019631 | 0.013604 | 0.078170 | 0.007645 | 0.018120 | 0.019784 | 0.089741 |
None | 0.009633 | 0.002582 | 0.000130 | 0.000402 | 0.001246 | 0.000929 | 0.000046 | 0.000126 | 0.006308 | 0.001716 | 0.000079 | 0.000379 |
Nuclear Periphery | 0.301002* | 0.358732* | 0.576089* | 0.215335* | 0.299643* | 0.480186* | 0.616017* | 0.242236* | 0.300463* | 0.422341* | 0.600725* | 0.217639* |
Nuclear Periphery Foci | 0.488888* | 0.531352* | 0.352425* | 0.558518* | 0.621856* | 0.445413* | 0.346084* | 0.544854* | 0.541597* | 0.486344* | 0.348513* | 0.557348* |
Nucleolus | 0.003836 | 0.002071 | 0.003618 | 0.000122 | 0.002284 | 0.001563 | 0.000247 | 0.000070 | 0.003221 | 0.001805 | 0.001538 | 0.000117 |
Nucleus | 0.096541 | 0.012582 | 0.005941 | 0.001539 | 0.006916 | 0.009511 | 0.000908 | 0.000611 | 0.061014 | 0.010973 | 0.002836 | 0.001460 |
Peroxisomes | 0.000091 | 0.000031 | 0.000110 | 0.000053 | 0.000037 | 0.000201 | 0.000007 | 0.000022 | 0.000070 | 0.000120 | 0.000047 | 0.000050 |
Vacuole | 0.012182 | 0.017959 | 0.005889 | 0.054641 | 0.008998 | 0.007812 | 0.008049 | 0.058087 | 0.010920 | 0.012645 | 0.007222 | 0.054936 |
Vacuole Periphery | 0.018199 | 0.015534 | 0.003967 | 0.017345 | 0.011475 | 0.009136 | 0.004290 | 0.013342 | 0.015534 | 0.012183 | 0.004166 | 0.017002 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 5.22 | 4.82 | 7.80 | 2.20 | 1.72 | 0.26 | 2.48 | -0.28 | -0.36 | -1.33 | 5.73 | 6.50 | 7.44 | 2.16 | 0.58 |
Bud Neck | -0.22 | -3.19 | -6.31 | -5.91 | 2.55 | 1.56 | -3.09 | -3.72 | -3.83 | -3.18 | 1.12 | -3.75 | -7.18 | -7.40 | 1.54 |
Bud Site | 0.29 | -0.87 | -4.39 | -4.45 | -2.33 | -1.12 | 4.08 | -2.66 | -2.49 | -2.83 | -0.79 | 0.95 | -5.33 | -4.53 | -5.43 |
Cell Periphery | 1.89 | 0.87 | -0.43 | -2.16 | -1.07 | 3.88 | 2.90 | -3.04 | -3.57 | -3.52 | 3.97 | 2.99 | -3.13 | -5.95 | -4.95 |
Cytoplasm | -2.46 | 7.46 | -2.16 | 0.68 | -7.98 | 0.54 | 5.11 | 2.52 | 2.12 | -2.02 | -1.38 | 9.10 | -2.10 | -0.75 | -8.33 |
Cytoplasmic Foci | 0.73 | 1.48 | -2.02 | -2.26 | -2.75 | 0.07 | 6.77 | -1.98 | -1.85 | -3.21 | 0.42 | 5.78 | -2.44 | -2.34 | -4.70 |
Eisosomes | 6.50 | 0.11 | 7.15 | 0.08 | 1.94 | 0.27 | 3.03 | -1.48 | -1.52 | -2.00 | 6.05 | 1.94 | 5.02 | -0.80 | 0.66 |
Endoplasmic Reticulum | 5.68 | 11.61 | 3.05 | -1.35 | -5.17 | 2.69 | 5.59 | 3.04 | 1.05 | -2.01 | 6.68 | 12.50 | 2.26 | -2.05 | -5.50 |
Endosome | 1.71 | 6.09 | -3.82 | -4.92 | -7.97 | 3.31 | 6.48 | -2.55 | -3.75 | -4.71 | 4.02 | 10.11 | -4.46 | -7.11 | -10.66 |
Golgi | -0.36 | 5.24 | -8.12 | -6.05 | -10.12 | 1.04 | 6.38 | -2.85 | -3.00 | -3.31 | 0.41 | 7.96 | -8.93 | -8.25 | -10.88 |
Lipid Particles | 8.63 | 8.22 | -0.87 | -8.82 | -8.49 | 5.11 | 4.81 | -3.64 | -5.43 | -5.38 | 10.25 | 10.05 | -3.15 | -10.32 | -10.28 |
Mitochondria | 7.23 | 9.36 | 9.49 | 2.74 | 0.31 | -2.10 | 2.35 | 5.32 | 4.58 | 1.29 | 6.84 | 9.64 | 9.50 | 3.91 | -0.68 |
Mitotic Spindle | -4.20 | -2.39 | -16.63 | -13.63 | -5.27 | -3.00 | -0.65 | -4.15 | -3.57 | -3.78 | -5.37 | -2.48 | -16.86 | -14.28 | -10.13 |
None | 5.60 | 8.19 | 7.83 | 4.54 | -3.57 | 1.08 | 5.70 | 5.23 | 3.77 | -1.39 | 6.08 | 8.81 | 8.16 | 4.85 | -4.60 |
Nuclear Periphery | -5.93 | -10.77 | 12.50 | 16.41 | 14.74 | -18.42* | -18.26 | 3.55 | 12.53 | 15.75 | -17.49 | -20.87 | 14.17 | 29.08* | 26.99 |
Nuclear Periphery Foci | -3.91 | 6.82 | -5.28 | -1.01 | -9.37 | 18.23* | 17.41 | 4.06 | -3.66 | -6.98 | 7.58 | 15.43 | 0.23 | -6.43 | -14.55 |
Nucleolus | 3.09 | -0.50 | 9.53 | 4.86 | 1.67 | 1.03 | 3.81 | 4.19 | 3.71 | 2.12 | 3.25 | 0.79 | 9.76 | 5.83 | 1.71 |
Nucleus | 13.08 | 11.43 | 15.25 | 4.45 | 1.44 | -1.30 | 4.14 | 4.92 | 4.80 | 0.42 | 12.34 | 13.53 | 15.06 | 5.24 | 0.61 |
Peroxisomes | 4.54 | -0.95 | 1.35 | -0.74 | 1.29 | -1.41 | 3.43 | 0.80 | 1.50 | -1.72 | -0.82 | -0.22 | 0.76 | 1.06 | 0.60 |
Vacuole | -2.96 | 3.58 | -15.06 | -11.54 | -16.16 | 0.74 | 0.31 | -5.60 | -5.68 | -5.57 | -1.63 | 2.06 | -16.60 | -15.26 | -16.25 |
Vacuole Periphery | 1.41 | 11.28 | 0.83 | -0.68 | -9.39 | 1.36 | 5.86 | -0.44 | -1.05 | -2.42 | 2.61 | 12.86 | -0.75 | -2.87 | -10.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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