Standard name | |
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Human Ortholog | |
Description | Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 5 | 10 |
Bud | 2 | 2 | 4 | 1 | 1 | 2 | 8 | 6 | 5 | 5 | 4 | 3 | 5 | 0 | 0 | 1 | 3 | 0 | 6 | 8 | 4 | 6 | 13 | 11 |
Bud Neck | 12 | 7 | 15 | 4 | 10 | 7 | 26 | 19 | 14 | 12 | 11 | 10 | 2 | 0 | 1 | 8 | 7 | 8 | 8 | 9 | 3 | 6 | 4 | 8 |
Bud Site | 8 | 4 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 2 | 2 | 2 | – | – | – | – | – | – |
Cell Periphery | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 1 | 1 | 2 | 4 | 8 |
Cytoplasm | 244 | 195 | 59 | 18 | 59 | 52 | 138 | 167 | 122 | 122 | 52 | 46 | 249 | 346 | 366 | 163 | 192 | 161 | 165 | 133 | 43 | 138 | 252 | 332 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 9 | 9 | 13 | 0 | 0 | 0 | 3 | 5 | 3 |
Endosome | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 8 | 4 | 13 | 15 | 16 | 13 | 11 | 0 | 3 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 9 | 38 | 2 | 0 | 1 | 10 | 20 | 18 | 46 | 37 | 18 | 19 | 8 | 1 | 4 | 11 | 12 | 4 | 17 | 17 | 6 | 50 | 53 | 49 |
Nucleus | 5 | 5 | 2 | 1 | 2 | 2 | 3 | 3 | 4 | 4 | 2 | 1 | 0 | 1 | 0 | 4 | 6 | 0 | 1 | 3 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 6 | 6 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 18 | 9 | 7 | 8 | 9 | 1 | 1 | 0 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 3 | 3 | 4 | 8 | 1 | 0 | 0 | 1 | 0 | 1 |
SpindlePole | 43 | 27 | 9 | 5 | 17 | 5 | 26 | 27 | 6 | 8 | 8 | 11 | 6 | 8 | 5 | 51 | 63 | 69 | 90 | 77 | 34 | 18 | 3 | 6 |
Vac/Vac Membrane | 3 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 11 | 9 | 3 | 8 | 7 | 1 | 7 | 16 | 15 |
Unique Cell Count | 318 | 261 | 82 | 26 | 80 | 68 | 180 | 213 | 158 | 164 | 81 | 78 | 273 | 361 | 376 | 242 | 289 | 250 | 339 | 293 | 106 | 252 | 373 | 463 |
Labelled Cell Count | 345 | 294 | 95 | 31 | 91 | 81 | 224 | 244 | 200 | 192 | 97 | 96 | 281 | 372 | 386 | 300 | 334 | 292 | 339 | 293 | 106 | 252 | 373 | 463 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.6 | 4.3 | 4.2 | 4.6 | 3.5 | 3.6 | 3.7 | 3.0 | 3.1 | 3.1 | 3.3 | 3.8 | 4.2 | 4.1 | 5.9 | 5.9 | 5.9 | 3.6 | 4.0 | 4.1 |
Std Deviation (1e-4) | 1.0 | 0.6 | 1.2 | 0.8 | 1.8 | 1.3 | 1.0 | 1.2 | 1.0 | 1.0 | 1.5 | 1.1 | 1.4 | 2.4 | 1.8 | 1.5 | 1.6 | 1.7 | 1.1 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.1 | -0.29 | -0.28 | -0.22 | -0.52 | -0.51 | -0.47 | -0.39 | -0.21 | -0.05 | -0.08 | 0.44 | 0.44 | 0.45 | -0.26 | -0.14 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3093 | 1.7226 | 1.4438 | 1.5516 | 1.8063 | 1.7596 | -0.7803 | -0.6915 | -0.5077 | -0.7725 | -0.1549 | -0.578 | -0.7811 | -0.2042 | -0.4341 | 0.3541 | 0.2478 | -0.3278 |
Actin | 0.0488 | 0.0001 | 0.0107 | 0.0005 | 0.0391 | 0.0003 | 0.0551 | 0.0009 | 0.0105 | 0 | 0.04 | 0.0066 | 0.0122 | 0.0005 | 0.0059 | 0.0006 | 0.0056 | 0.006 |
Bud | 0.001 | 0.0004 | 0.003 | 0.0004 | 0.0011 | 0.0016 | 0.0057 | 0.0136 | 0.0011 | 0 | 0.0035 | 0.0008 | 0.0067 | 0.0219 | 0.0106 | 0.0017 | 0.0006 | 0.0029 |
Bud Neck | 0.0073 | 0.0008 | 0.0107 | 0.0251 | 0.0823 | 0.0586 | 0.001 | 0.0015 | 0.0007 | 0.0017 | 0.1024 | 0.0417 | 0.0027 | 0.002 | 0.0029 | 0.0382 | 0.1612 | 0.0526 |
Bud Periphery | 0.0021 | 0.0006 | 0.0048 | 0.0008 | 0.0021 | 0.0017 | 0.0068 | 0.012 | 0.0022 | 0 | 0.0058 | 0.0013 | 0.0055 | 0.0072 | 0.0057 | 0.0018 | 0.0007 | 0.0017 |
Bud Site | 0.0114 | 0.0039 | 0.0035 | 0.0008 | 0.0066 | 0.0005 | 0.0081 | 0.0256 | 0.0023 | 0.0001 | 0.004 | 0.0007 | 0.0131 | 0.0369 | 0.0227 | 0.0073 | 0.0059 | 0.0004 |
Cell Periphery | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0 | 0.0006 | 0.0002 | 0.0007 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0002 |
Cytoplasm | 0.0124 | 0.0263 | 0.0247 | 0.0065 | 0.0013 | 0.0429 | 0.0908 | 0.0972 | 0.0564 | 0.0003 | 0.0029 | 0.1283 | 0.0304 | 0.0608 | 0.0275 | 0.0026 | 0.0048 | 0.0871 |
Cytoplasmic Foci | 0.1163 | 0.0443 | 0.1107 | 0.0225 | 0.135 | 0.0624 | 0.1188 | 0.0713 | 0.2079 | 0.121 | 0.1127 | 0.1426 | 0.1434 | 0.0554 | 0.0912 | 0.0622 | 0.0958 | 0.1065 |
Eisosomes | 0.0025 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0 | 0.001 | 0.0001 |
Endoplasmic Reticulum | 0.0035 | 0.0004 | 0.0013 | 0.0003 | 0.0036 | 0.0005 | 0.0039 | 0.0016 | 0.0024 | 0 | 0.0011 | 0.0012 | 0.0043 | 0.0024 | 0.0012 | 0.0001 | 0.0024 | 0.0026 |
Endosome | 0.013 | 0.0032 | 0.004 | 0.0016 | 0.0216 | 0.0039 | 0.0292 | 0.0102 | 0.0208 | 0.0003 | 0.0147 | 0.0101 | 0.0081 | 0.0052 | 0.0033 | 0.0006 | 0.0144 | 0.0059 |
Golgi | 0.0139 | 0.0003 | 0.004 | 0.0004 | 0.0105 | 0.0009 | 0.0149 | 0.0009 | 0.0148 | 0.0003 | 0.0311 | 0.0034 | 0.003 | 0.0005 | 0.0008 | 0.0004 | 0.0032 | 0.0016 |
Lipid Particles | 0.0197 | 0.0056 | 0.0103 | 0.0026 | 0.0109 | 0.0385 | 0.0309 | 0.0047 | 0.0267 | 0.0012 | 0.0088 | 0.0396 | 0.0139 | 0.0066 | 0.0034 | 0.0019 | 0.0194 | 0.0258 |
Mitochondria | 0.0065 | 0.0001 | 0.0025 | 0.0006 | 0.0076 | 0.0075 | 0.0169 | 0.0013 | 0.0107 | 0 | 0.0379 | 0.0172 | 0.0032 | 0.001 | 0.0013 | 0.0002 | 0.0079 | 0.0037 |
None | 0.62 | 0.7348 | 0.4397 | 0.0789 | 0.0034 | 0.6707 | 0.5337 | 0.5326 | 0.2464 | 0.0006 | 0.0105 | 0.4669 | 0.5808 | 0.5759 | 0.3183 | 0.1228 | 0.0279 | 0.5347 |
Nuclear Periphery | 0.0034 | 0.0006 | 0.0013 | 0.0013 | 0.0251 | 0.0029 | 0.0047 | 0.0019 | 0.0022 | 0.0004 | 0.0005 | 0.0025 | 0.0041 | 0.0036 | 0.0032 | 0.0006 | 0.0077 | 0.0059 |
Nucleolus | 0.0041 | 0.0134 | 0.0106 | 0.0284 | 0.0248 | 0.0138 | 0.0006 | 0.0261 | 0.002 | 0.0039 | 0.0473 | 0.0066 | 0.0093 | 0.0079 | 0.0077 | 0.0043 | 0.0446 | 0.0027 |
Nucleus | 0.0032 | 0.0178 | 0.0249 | 0.0216 | 0.0052 | 0.0429 | 0.0125 | 0.0434 | 0.0033 | 0.0013 | 0.01 | 0.0417 | 0.0075 | 0.0194 | 0.023 | 0.0039 | 0.0185 | 0.0241 |
Peroxisomes | 0.0234 | 0.0008 | 0.0133 | 0.0006 | 0.0218 | 0.011 | 0.0374 | 0.0027 | 0.0125 | 0.0014 | 0.0031 | 0.0202 | 0.0085 | 0.0014 | 0.002 | 0.001 | 0.0075 | 0.0125 |
Punctate Nuclear | 0.081 | 0.1458 | 0.3187 | 0.8065 | 0.5926 | 0.0373 | 0.0099 | 0.148 | 0.3747 | 0.8675 | 0.5584 | 0.065 | 0.1393 | 0.1877 | 0.4675 | 0.7495 | 0.5669 | 0.1213 |
Vacuole | 0.0042 | 0.0005 | 0.0005 | 0.0005 | 0.0017 | 0.0013 | 0.0094 | 0.003 | 0.0014 | 0 | 0.0035 | 0.0022 | 0.0022 | 0.0025 | 0.001 | 0.0003 | 0.0022 | 0.0012 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0003 | 0.0001 | 0.0027 | 0.0004 | 0.0087 | 0.0003 | 0.0005 | 0 | 0.0008 | 0.0009 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0017 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.6042 | 26.719 | 23.6818 | 22.974 | 7.2661 | 8.7128 | 30.9553 | 35.8342 | 25.6453 | 11.2337 |
Translational Efficiency | 0.4348 | 0.606 | 0.7927 | 0.6011 | 0.5163 | 0.23 | 0.6281 | 0.6337 | 0.6557 | 0.6119 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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