Standard name | |
---|---|
Human Ortholog | |
Description | Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 3 | 2 | 3 | 0 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 5 | 1 | 0 | 2 | 0 | 4 |
Bud | 8 | 1 | 4 | 2 | 9 | 22 | 20 | 31 | 19 | 20 | 16 | 25 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 6 | 1 | 2 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 40 | 15 | 4 | 13 | 21 | 27 | 30 | 38 | 22 | 42 | 18 | 35 | 6 | 14 | 10 | 16 | 9 | 15 | 11 | 1 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 2 | 1 | 2 | 1 | 1 | 5 | 5 | 4 | 7 | 6 | 12 | 12 | 3 | 1 | 0 | 11 | 3 | 8 | 2 | 0 | 0 | 5 | 8 | 4 |
Endosome | 214 | 341 | 245 | 234 | 266 | 204 | 120 | 278 | 97 | 185 | 93 | 103 | 223 | 324 | 303 | 189 | 205 | 176 | 315 | 502 | 296 | 234 | 235 | 261 |
Golgi | 5 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 1 | 4 | 11 | 10 | 6 | 7 | 15 | 15 |
Mitochondria | 46 | 37 | 65 | 51 | 99 | 161 | 261 | 254 | 232 | 310 | 247 | 362 | 6 | 5 | 0 | 34 | 39 | 17 | 8 | 9 | 4 | 6 | 9 | 10 |
Nucleus | 6 | 5 | 5 | 0 | 0 | 3 | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 2 | 3 | 1 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 8 | 6 | 2 | 0 | 1 | 0 | 0 | 1 | 4 | 1 | 3 | 5 | 3 | 1 | 0 | 3 | 5 | 1 | 3 | 1 | 1 | 1 | 2 |
Nucleolus | 55 | 92 | 69 | 27 | 21 | 30 | 26 | 51 | 24 | 29 | 15 | 33 | 46 | 47 | 74 | 48 | 79 | 62 | 1 | 4 | 1 | 1 | 1 | 1 |
Peroxisomes | 4 | 1 | 12 | 13 | 11 | 14 | 9 | 44 | 12 | 10 | 4 | 9 | 8 | 13 | 8 | 3 | 6 | 4 | 2 | 3 | 3 | 1 | 1 | 3 |
SpindlePole | 78 | 58 | 44 | 34 | 32 | 50 | 50 | 109 | 37 | 44 | 14 | 31 | 83 | 109 | 100 | 83 | 77 | 63 | 18 | 18 | 14 | 11 | 11 | 9 |
Vac/Vac Membrane | 42 | 299 | 50 | 91 | 131 | 70 | 53 | 61 | 63 | 62 | 40 | 46 | 101 | 109 | 99 | 66 | 60 | 62 | 23 | 60 | 8 | 21 | 13 | 16 |
Unique Cell Count | 368 | 581 | 325 | 305 | 387 | 425 | 429 | 623 | 391 | 546 | 375 | 529 | 307 | 391 | 352 | 242 | 264 | 240 | 414 | 632 | 343 | 301 | 305 | 340 |
Labelled Cell Count | 502 | 863 | 509 | 470 | 591 | 588 | 576 | 874 | 517 | 719 | 464 | 664 | 486 | 628 | 597 | 450 | 484 | 420 | 414 | 632 | 343 | 301 | 305 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 7.8 | 6.4 | 6.5 | 6.0 | 5.6 | 5.0 | 5.4 | 5.1 | 4.9 | 5.0 | 5.1 | 8.2 | 8.0 | 8.5 | 8.8 | 8.9 | 8.9 | 9.1 | 9.2 | 9.2 |
Std Deviation (1e-4) | 1.6 | 1.1 | 1.1 | 1.2 | 1.1 | 0.9 | 0.8 | 1.2 | 0.8 | 0.9 | 1.0 | 0.8 | 1.1 | 1.1 | 1.2 | 1.5 | 1.6 | 1.7 | 1.4 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.09 | -0.17 | -0.35 | -0.22 | -0.31 | -0.37 | -0.34 | -0.31 | 0.37 | 0.33 | 0.42 | 0.47 | 0.48 | 0.48 | 0.52 | 0.54 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1.1 | 2.9 | 2.7 | 2.9 | 2.7 | 2.1 | 2.4 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.3 | 3.0 | 3.5 | 3.8 | 3.5 | 3.1 | 4.1 | 2.7 | 3.7 | 0 | 2.0 | 1.5 | 3.4 | 1.8 | 3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.8 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Endosome | 0.4 | -2.0 | -7.6 | -12.9 | -9.0 | -13.5 | -11.8 | -13.4 | -16.1 | -0.8 | 2.5 | 3.5 | 0.8 | 0.6 | -0.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.1 | 1.8 | 5.3 | 11.2 | 6.4 | 10.6 | 10.6 | 12.2 | 13.7 | -7.2 | -8.4 | -8.8 | -1.8 | -1.7 | -4.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -4.3 | -6.3 | -5.7 | -6.2 | -5.7 | -6.0 | -7.2 | -7.0 | -6.6 | -2.0 | -3.3 | -0.1 | -0.4 | 2.4 | 1.3 |
Peroxisomes | 0.4 | -0.6 | -0.3 | -1.3 | 2.1 | -0.5 | -1.7 | -2.3 | -1.8 | -0.8 | -0.3 | -1.1 | -1.8 | -1.0 | -1.4 |
SpindlePole | -0.9 | -2.3 | -0.7 | -0.8 | 1.6 | -1.7 | -2.6 | -4.7 | -3.8 | 4.2 | 4.7 | 4.7 | 5.9 | 4.7 | 3.8 |
Vacuole | 4.3 | 5.6 | 0.4 | -1.2 | -2.5 | 0.3 | -1.7 | -1.9 | -3.0 | 5.2 | 4.0 | 4.0 | 3.5 | 2.3 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8044 | 3.6911 | 3.2409 | 3.1578 | 2.6541 | 3.3215 | 4.9833 | 6.6308 | 5.9783 | 5.6811 | 5.7497 | 6.0109 | 4.4589 | 5.7647 | 5.0977 | 5.1115 | 4.4184 | 5.086 |
Actin | 0.0014 | 0.0001 | 0.0052 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0021 | 0.0012 | 0 | 0.0006 | 0.0022 | 0 | 0.0002 |
Bud | 0.0001 | 0.001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0.001 | 0.0005 | 0.0001 | 0.0006 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0015 | 0.0012 | 0.0038 | 0.0006 | 0.0011 | 0.0004 |
Bud Periphery | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0008 | 0.0054 | 0.0014 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0019 | 0.0007 | 0.0004 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0029 | 0.017 | 0.0048 | 0.0015 | 0.0015 | 0.0022 | 0.0013 | 0.0021 | 0.0011 | 0.0013 | 0.0009 | 0.0015 | 0.0075 | 0.0036 | 0.0025 | 0.0011 | 0.0011 | 0.0016 |
Cytoplasmic Foci | 0.1244 | 0.153 | 0.1458 | 0.1524 | 0.1355 | 0.1556 | 0.1371 | 0.183 | 0.1758 | 0.118 | 0.1064 | 0.1437 | 0.0919 | 0.1469 | 0.1178 | 0.0884 | 0.1418 | 0.1101 |
Eisosomes | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0.001 | 0.0013 | 0.0003 | 0.0008 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0017 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0008 |
Endosome | 0.6436 | 0.6103 | 0.6373 | 0.7223 | 0.5823 | 0.6121 | 0.6448 | 0.6042 | 0.6084 | 0.7544 | 0.7195 | 0.5768 | 0.7084 | 0.6482 | 0.7106 | 0.7994 | 0.762 | 0.6589 |
Golgi | 0.0492 | 0.0364 | 0.0616 | 0.0462 | 0.095 | 0.064 | 0.0596 | 0.0809 | 0.0955 | 0.0758 | 0.0636 | 0.0861 | 0.041 | 0.0413 | 0.0594 | 0.0376 | 0.0495 | 0.0622 |
Lipid Particles | 0.1103 | 0.0525 | 0.0638 | 0.018 | 0.0939 | 0.1075 | 0.117 | 0.0864 | 0.0755 | 0.018 | 0.0793 | 0.1497 | 0.0781 | 0.0764 | 0.0587 | 0.0354 | 0.0184 | 0.1053 |
Mitochondria | 0.0099 | 0.001 | 0.0092 | 0.0095 | 0.0301 | 0.0014 | 0.0022 | 0.0093 | 0.002 | 0.0007 | 0.0005 | 0.0014 | 0.0072 | 0.0011 | 0.0031 | 0.0019 | 0.0003 | 0.0029 |
None | 0.0005 | 0.0003 | 0.0034 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0046 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 |
Nuclear Periphery | 0.0018 | 0.0067 | 0.0022 | 0.0003 | 0.0017 | 0.0006 | 0.004 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0033 | 0.0026 | 0.0006 | 0.0015 | 0.0003 | 0.0001 | 0.003 |
Nucleolus | 0.0004 | 0.0018 | 0.0009 | 0.0001 | 0.0007 | 0.0019 | 0.0004 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0019 | 0.0031 | 0.0007 | 0.0004 | 0.0014 | 0.0009 |
Nucleus | 0.0003 | 0.002 | 0.0015 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0037 | 0.0005 | 0.0023 | 0.0001 | 0.0001 | 0.0012 |
Peroxisomes | 0.0294 | 0.0138 | 0.0233 | 0.0085 | 0.0257 | 0.0167 | 0.0152 | 0.0213 | 0.023 | 0.0067 | 0.0053 | 0.0164 | 0.0095 | 0.0091 | 0.01 | 0.0091 | 0.0089 | 0.0103 |
Punctate Nuclear | 0.0019 | 0.0012 | 0.0017 | 0.0002 | 0.0013 | 0.0049 | 0.0025 | 0.0005 | 0.0014 | 0.0001 | 0.0002 | 0.0008 | 0.0025 | 0.003 | 0.001 | 0.0006 | 0.0008 | 0.0012 |
Vacuole | 0.0164 | 0.0832 | 0.0285 | 0.0337 | 0.0205 | 0.0251 | 0.0104 | 0.0068 | 0.0126 | 0.0209 | 0.0191 | 0.0115 | 0.0284 | 0.0538 | 0.0196 | 0.0165 | 0.0104 | 0.0312 |
Vacuole Periphery | 0.0048 | 0.0114 | 0.0062 | 0.0062 | 0.0081 | 0.0059 | 0.0038 | 0.0031 | 0.0036 | 0.0032 | 0.0039 | 0.0046 | 0.0076 | 0.0097 | 0.0052 | 0.0049 | 0.0034 | 0.0092 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.2193 | 14.5914 | 22.7376 | 20.8478 | 15.8722 | 26.1016 | 19.6711 | 21.9065 | 23.3898 | 20.2773 |
Translational Efficiency | 1.4998 | 2.1066 | 1.2879 | 1.7507 | 1.8614 | 1.3743 | 1.5142 | 1.6945 | 1.569 | 1.4398 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1350 | 1458 | 175 | 1353 | 1693 | 1321 | 2853 | 1279 | 3043 | 2779 | 3028 | 2632 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 782.81 | 931.88 | 1118.60 | 1152.59 | 849.69 | 848.39 | 1047.60 | 1137.80 | 820.02 | 892.19 | 1051.70 | 1145.40 |
Standard Deviation | 128.41 | 151.44 | 126.86 | 186.47 | 106.50 | 127.91 | 134.00 | 171.39 | 121.37 | 146.79 | 134.62 | 179.45 |
Intensity Change Log 2 | — | 0.251482 | 0.514960 | 0.558145 | — | -0.002209 | 0.302079 | 0.421238 | — | 0.125013 | 0.408083 | 0.488510 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000042 | 0.000097 | 0.000188 | 0.000593 | 0.000018 | 0.000093 | 0.000033 | 0.000375 | 0.000029 | 0.000095 | 0.000042 | 0.000487 |
Bud Neck | 0.002637 | 0.001188 | 0.013874 | 0.001128 | 0.000785 | 0.002469 | 0.000744 | 0.001179 | 0.001607 | 0.001797 | 0.001502 | 0.001153 |
Bud Site | 0.002415 | 0.003712 | 0.012265 | 0.017856 | 0.001906 | 0.006787 | 0.005423 | 0.015838 | 0.002132 | 0.005174 | 0.005818 | 0.016875 |
Cell Periphery | 0.000061 | 0.000034 | 0.000152 | 0.000143 | 0.000021 | 0.000055 | 0.000055 | 0.000111 | 0.000039 | 0.000044 | 0.000061 | 0.000128 |
Cytoplasm | 0.004136 | 0.003063 | 0.002482 | 0.014218 | 0.002201 | 0.006606 | 0.005265 | 0.014458 | 0.003060 | 0.004747 | 0.005104 | 0.014335 |
Cytoplasmic Foci | 0.073946 | 0.077345 | 0.072040 | 0.066054 | 0.073395 | 0.105586 | 0.054193 | 0.079806 | 0.073639 | 0.090769 | 0.055224 | 0.072736 |
Eisosomes | 0.000015 | 0.000008 | 0.000032 | 0.000010 | 0.000005 | 0.000014 | 0.000004 | 0.000009 | 0.000010 | 0.000011 | 0.000005 | 0.000010 |
Endoplasmic Reticulum | 0.000250 | 0.000172 | 0.000817 | 0.000211 | 0.000158 | 0.000248 | 0.000078 | 0.000217 | 0.000199 | 0.000208 | 0.000121 | 0.000214 |
Endosome | 0.760566* | 0.726328* | 0.461325* | 0.328061* | 0.753281* | 0.686209* | 0.476332* | 0.386099* | 0.756513* | 0.707257* | 0.475465* | 0.356264* |
Golgi | 0.053646 | 0.064156 | 0.019279 | 0.201543* | 0.078366 | 0.071114 | 0.101818 | 0.212860* | 0.067399 | 0.067464 | 0.097048 | 0.207043* |
Lipid Particles | 0.013318 | 0.005396 | 0.008280 | 0.001209 | 0.004062 | 0.009974 | 0.000462 | 0.000748 | 0.008168 | 0.007572 | 0.000914 | 0.000985 |
Mitochondria | 0.007462 | 0.005933 | 0.001095 | 0.005408 | 0.005546 | 0.009407 | 0.008233 | 0.007102 | 0.006396 | 0.007584 | 0.007821 | 0.006231 |
Mitotic Spindle | 0.000795 | 0.004178 | 0.029093 | 0.012049 | 0.001532 | 0.011517 | 0.005686 | 0.008278 | 0.001205 | 0.007667 | 0.007038 | 0.010217 |
None | 0.000233 | 0.000033 | 0.000058 | 0.000039 | 0.000034 | 0.000117 | 0.000071 | 0.000117 | 0.000122 | 0.000073 | 0.000070 | 0.000077 |
Nuclear Periphery | 0.000204 | 0.000259 | 0.002937 | 0.000361 | 0.000111 | 0.000374 | 0.000285 | 0.000134 | 0.000152 | 0.000314 | 0.000438 | 0.000251 |
Nuclear Periphery Foci | 0.009105 | 0.007594 | 0.033227 | 0.001657 | 0.002677 | 0.006140 | 0.001326 | 0.000945 | 0.005528 | 0.006903 | 0.003170 | 0.001311 |
Nucleolus | 0.001562 | 0.000494 | 0.013705 | 0.000092 | 0.000082 | 0.001063 | 0.000205 | 0.000074 | 0.000738 | 0.000765 | 0.000985 | 0.000083 |
Nucleus | 0.001069 | 0.000331 | 0.006642 | 0.000083 | 0.000055 | 0.000743 | 0.000507 | 0.000191 | 0.000505 | 0.000527 | 0.000862 | 0.000136 |
Peroxisomes | 0.010622 | 0.004535 | 0.026328 | 0.001969 | 0.002478 | 0.009053 | 0.001048 | 0.003082 | 0.006091 | 0.006682 | 0.002509 | 0.002510 |
Vacuole | 0.040607 | 0.070861 | 0.245145* | 0.254895* | 0.051729 | 0.053834 | 0.241626* | 0.190533 | 0.046795 | 0.062767 | 0.241829* | 0.223619* |
Vacuole Periphery | 0.017310 | 0.024284 | 0.051035 | 0.092419 | 0.021557 | 0.018599 | 0.096607 | 0.077844 | 0.019672 | 0.021581 | 0.093973 | 0.085337 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.67 | -3.77 | -3.00 | -2.67 | -2.14 | -4.14 | -1.59 | -1.84 | -1.43 | -1.74 | -3.49 | -1.53 | -3.44 | -2.91 | -3.31 |
Bud Neck | 3.15 | -5.68 | 3.33 | 0.04 | 6.49 | -4.66 | 0.02 | -1.77 | 3.77 | -2.63 | -0.81 | 0.10 | 1.77 | 3.21 | 2.21 |
Bud Site | -2.02 | -5.55 | -10.40 | -9.09 | -2.27 | -4.68 | -5.82 | -10.28 | -5.35 | -6.95 | -5.08 | -6.57 | -14.62 | -10.27 | -9.47 |
Cell Periphery | 3.22 | -2.88 | -4.94 | -7.48 | 0.23 | -3.84 | -3.79 | -8.92 | -3.37 | -3.49 | -1.32 | -2.55 | -9.22 | -7.82 | -4.62 |
Cytoplasm | 0.93 | 1.16 | -6.46 | -7.52 | -7.70 | -3.19 | -4.45 | -6.56 | -3.57 | -4.72 | -1.97 | -3.14 | -9.42 | -7.42 | -7.36 |
Cytoplasmic Foci | -0.84 | 0.25 | 2.21 | 3.25 | 0.80 | -7.32 | 6.77 | -1.86 | 5.79 | -8.77 | -5.80 | 7.72 | 0.39 | 6.41 | -7.88 |
Eisosomes | 4.61 | -4.04 | 2.82 | -1.43 | 5.13 | -7.81 | 3.75 | -3.62 | 4.17 | -5.71 | -1.63 | 6.07 | 0.24 | 1.74 | -5.09 |
Endoplasmic Reticulum | 1.63 | -4.32 | 0.77 | -1.14 | 4.73 | -1.52 | 1.64 | -0.97 | 0.59 | -3.64 | -0.25 | 2.21 | -0.39 | -0.20 | -3.66 |
Endosome | 3.90 | 14.19 | 48.29* | 45.92* | 5.98 | 7.80 | 37.92* | 43.49* | 30.96 | 10.72 | 8.11 | 43.87* | 65.29* | 53.93* | 17.63 |
Golgi | -3.22 | 10.97 | -23.01 | -21.30 | -28.66* | 1.87 | -6.24 | -19.65 | -20.35 | -16.28 | 0.01 | -9.59 | -29.61 | -29.46 | -21.89 |
Lipid Particles | 4.75 | 1.98 | 7.62 | 5.25 | 3.29 | -4.32 | 4.63 | 4.22 | 8.08 | -3.23 | 0.57 | 8.88 | 8.55 | 9.63 | -0.93 |
Mitochondria | 1.03 | 5.54 | 1.58 | 0.48 | -5.61 | -2.62 | -2.73 | -1.00 | 1.26 | 0.77 | -1.16 | -1.55 | 0.22 | 1.28 | 1.64 |
Mitotic Spindle | -3.90 | -3.37 | -7.20 | -4.51 | 2.03 | -5.71 | -5.59 | -4.51 | 1.24 | -1.67 | -6.78 | -7.04 | -8.29 | -2.04 | -2.40 |
None | 2.46 | 2.12 | 2.40 | -0.66 | 1.84 | -2.99 | -2.22 | -1.02 | 0.04 | -0.51 | 1.27 | 1.26 | 0.89 | -0.05 | -0.07 |
Nuclear Periphery | -0.80 | -5.22 | -1.38 | -1.02 | 4.85 | -3.89 | -5.41 | -0.92 | 3.42 | 4.25 | -3.54 | -5.81 | -1.69 | 0.85 | 2.75 |
Nuclear Periphery Foci | 0.62 | -2.84 | 4.37 | 3.97 | 3.70 | -2.61 | 1.75 | 2.12 | 4.43 | 1.02 | -1.17 | 2.18 | 4.77 | 5.82 | 3.18 |
Nucleolus | 1.73 | -3.25 | 2.49 | 2.26 | 3.67 | -2.19 | -2.81 | 0.39 | 2.21 | 3.04 | -0.10 | -0.82 | 2.49 | 2.92 | 4.02 |
Nucleus | 1.27 | -2.45 | 1.75 | 1.42 | 2.96 | -3.07 | -2.06 | -1.16 | 2.26 | 1.35 | -0.18 | -1.12 | 1.43 | 2.66 | 2.85 |
Peroxisomes | 5.96 | -3.65 | 9.48 | 4.63 | 5.77 | -7.53 | 5.73 | -1.14 | 6.14 | -4.16 | -0.97 | 7.13 | 7.21 | 7.60 | 0.02 |
Vacuole | -7.83 | -12.81 | -33.41 | -27.64 | -0.76 | -0.57 | -40.86 | -23.01 | -21.94 | 7.42 | -5.91 | -46.20 | -39.64 | -34.84 | 2.91 |
Vacuole Periphery | -3.40 | -5.89 | -22.56* | -20.13 | -6.68 | 1.69 | -29.12* | -17.05 | -17.92 | 5.00 | -1.39 | -31.60 | -27.98 | -26.85 | 2.98 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|