Standard name | |
---|---|
Human Ortholog | |
Description | t-SNARE protein required for retrograde vesicular traffic; involved in Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 20 | 6 | 3 | 1 | 3 | 0 | 0 | 2 | 0 | 2 | 1 | 8 | 35 | 18 | 13 | 9 | 9 | 23 | 35 | 32 | 27 | 47 | 64 |
Bud | 4 | 5 | 10 | 7 | 15 | 28 | 47 | 21 | 24 | 25 | 43 | 46 | 4 | 10 | 9 | 4 | 3 | 2 | 2 | 5 | 2 | 9 | 5 | 16 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 5 | 10 | 4 | 6 | 7 | 19 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 3 | 2 | 8 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 1 | 2 | 7 | 7 | 11 | 3 | 9 | 7 | 7 | 10 | 0 | 7 | 3 | 2 | 0 | 2 | 1 | 2 | 1 | 1 | 2 | 3 |
Cytoplasm | 74 | 197 | 121 | 138 | 270 | 323 | 360 | 278 | 217 | 237 | 221 | 295 | 81 | 183 | 210 | 61 | 66 | 67 | 29 | 70 | 33 | 27 | 24 | 39 |
Endoplasmic Reticulum | 94 | 95 | 42 | 30 | 40 | 35 | 18 | 10 | 23 | 12 | 11 | 24 | 44 | 99 | 62 | 5 | 7 | 6 | 126 | 145 | 101 | 51 | 61 | 79 |
Endosome | 15 | 23 | 5 | 14 | 28 | 4 | 16 | 14 | 4 | 8 | 4 | 4 | 4 | 18 | 18 | 18 | 25 | 24 | 9 | 25 | 9 | 15 | 16 | 22 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 5 | 6 | 5 | 10 | 10 |
Mitochondria | 8 | 1 | 22 | 36 | 117 | 255 | 612 | 387 | 304 | 451 | 418 | 540 | 1 | 0 | 1 | 13 | 9 | 3 | 45 | 53 | 45 | 75 | 91 | 73 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 5 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 0 | 0 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 6 | 3 | 1 | 1 | 2 | 2 | 4 | 5 |
SpindlePole | 0 | 1 | 3 | 3 | 3 | 0 | 1 | 3 | 2 | 13 | 6 | 11 | 4 | 10 | 4 | 10 | 10 | 10 | 0 | 1 | 0 | 1 | 1 | 5 |
Vac/Vac Membrane | 12 | 17 | 0 | 2 | 11 | 18 | 21 | 20 | 18 | 37 | 11 | 12 | 2 | 11 | 7 | 9 | 12 | 15 | 14 | 25 | 11 | 17 | 13 | 29 |
Unique Cell Count | 197 | 328 | 188 | 208 | 435 | 552 | 914 | 638 | 484 | 668 | 577 | 751 | 136 | 329 | 310 | 114 | 125 | 123 | 270 | 390 | 256 | 247 | 295 | 375 |
Labelled Cell Count | 224 | 366 | 212 | 238 | 495 | 684 | 1095 | 753 | 607 | 807 | 732 | 954 | 148 | 375 | 335 | 139 | 151 | 145 | 270 | 390 | 256 | 247 | 295 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 6.4 | 4.7 | 4.6 | 4.4 | 4.2 | 3.7 | 3.9 | 3.8 | 3.6 | 3.6 | 3.7 | 6.5 | 6.2 | 6.4 | 6.6 | 6.9 | 6.8 | 7.6 | 7.2 | 7.3 |
Std Deviation (1e-4) | 1.0 | 1.3 | 0.9 | 1.2 | 1.2 | 1.1 | 1.2 | 1.2 | 1.3 | 1.0 | 0.9 | 0.8 | 1.3 | 1.0 | 1.4 | 1.3 | 1.5 | 1.4 | 2.5 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.08 | -0.18 | -0.36 | -0.25 | -0.31 | -0.37 | -0.37 | -0.35 | 0.47 | 0.4 | 0.44 | 0.49 | 0.55 | 0.53 | 0.69 | 0.62 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 3.0 | 1.3 | 2.8 | 1.6 | 1.7 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -1.3 | -1.4 | -0.7 | -1.3 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | -0.5 | -1.4 | -6.3 | -5.0 | -4.5 | -7.1 | -6.2 | -6.2 | -0.9 | -1.9 | 0.8 | -1.9 | -2.0 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.9 | -0.6 | -4.2 | -4.0 | -4.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.6 | 4.2 | 5.1 | 5.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.8 | -6.3 | -5.9 | -0.1 | -1.3 | -2.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.8 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4179 | 3.214 | 2.9483 | 1.9843 | 2.047 | 3.1361 | 4.187 | 5.7829 | 3.8909 | 2.6443 | 2.7252 | 3.6671 | 1.3982 | 3.0275 | 2.0631 | 1.1501 | 1.6917 | 3.0171 |
Actin | 0.0716 | 0.0196 | 0.0153 | 0.0228 | 0.0174 | 0.068 | 0.134 | 0.0019 | 0.1161 | 0.0015 | 0.0058 | 0.0623 | 0.0936 | 0.018 | 0.1348 | 0.0218 | 0.0262 | 0.0321 |
Bud | 0.0043 | 0.0077 | 0.013 | 0.0008 | 0.0087 | 0.0007 | 0.0026 | 0 | 0.0054 | 0.0002 | 0.0001 | 0.0009 | 0.0013 | 0.0007 | 0.0021 | 0.0139 | 0.0012 | 0.0003 |
Bud Neck | 0.0251 | 0.0017 | 0.0016 | 0.0021 | 0.0037 | 0.0234 | 0.0238 | 0.0002 | 0.0036 | 0.0005 | 0.0029 | 0.0112 | 0.0096 | 0.0015 | 0.0231 | 0.0342 | 0.0159 | 0.0013 |
Bud Periphery | 0.0033 | 0.0019 | 0.0071 | 0.0008 | 0.0029 | 0.0018 | 0.0024 | 0 | 0.0095 | 0.0003 | 0.0001 | 0.0008 | 0.002 | 0.0006 | 0.0033 | 0.0239 | 0.0021 | 0.0003 |
Bud Site | 0.0153 | 0.0482 | 0.0172 | 0.0021 | 0.0009 | 0.0041 | 0.0959 | 0.0001 | 0.0153 | 0.0004 | 0.0013 | 0.0014 | 0.0063 | 0.0291 | 0.0053 | 0.0182 | 0.0013 | 0.0011 |
Cell Periphery | 0.0022 | 0.0043 | 0.0021 | 0.0025 | 0.0008 | 0.0054 | 0.0009 | 0.0002 | 0.0011 | 0.0009 | 0.0008 | 0.0009 | 0.001 | 0.0016 | 0.0011 | 0.0046 | 0.0008 | 0.0002 |
Cytoplasm | 0.1437 | 0.3346 | 0.2597 | 0.0718 | 0.3622 | 0.2869 | 0.0195 | 0.0525 | 0.2771 | 0.2574 | 0.4746 | 0.2186 | 0.1797 | 0.2252 | 0.2052 | 0.1653 | 0.2731 | 0.1711 |
Cytoplasmic Foci | 0.1447 | 0.119 | 0.1178 | 0.1208 | 0.1446 | 0.1387 | 0.0606 | 0.0423 | 0.1201 | 0.0354 | 0.153 | 0.2049 | 0.1399 | 0.1698 | 0.1475 | 0.1361 | 0.0422 | 0.1526 |
Eisosomes | 0.001 | 0.0006 | 0.0012 | 0.0013 | 0.0003 | 0.0017 | 0.0009 | 0.0009 | 0.0005 | 0.0002 | 0.0011 | 0.0006 | 0.0013 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0669 | 0.0691 | 0.128 | 0.0903 | 0.0091 | 0.0615 | 0.0386 | 0.1625 | 0.0451 | 0.0646 | 0.0167 | 0.1079 | 0.0725 | 0.1264 | 0.0569 | 0.0796 | 0.1329 | 0.0907 |
Endosome | 0.1315 | 0.1297 | 0.1689 | 0.1653 | 0.0398 | 0.1679 | 0.2728 | 0.1078 | 0.1485 | 0.3326 | 0.0231 | 0.1799 | 0.1712 | 0.2198 | 0.1727 | 0.2217 | 0.2883 | 0.2738 |
Golgi | 0.0404 | 0.0428 | 0.0531 | 0.1027 | 0.0272 | 0.0753 | 0.1849 | 0.1126 | 0.084 | 0.1317 | 0.0056 | 0.1316 | 0.0833 | 0.0627 | 0.0856 | 0.0531 | 0.1051 | 0.0942 |
Lipid Particles | 0.0796 | 0.0963 | 0.065 | 0.0966 | 0.0496 | 0.035 | 0.0474 | 0.1206 | 0.0165 | 0.0277 | 0.0139 | 0.0224 | 0.1077 | 0.081 | 0.0725 | 0.1932 | 0.0319 | 0.1131 |
Mitochondria | 0.0458 | 0.0064 | 0.0121 | 0.085 | 0.1067 | 0.007 | 0.0595 | 0.0011 | 0.0043 | 0.0059 | 0.0004 | 0.0081 | 0.0141 | 0.0045 | 0.0137 | 0.0135 | 0.0287 | 0.0082 |
None | 0.1103 | 0.0717 | 0.0948 | 0.167 | 0.116 | 0.0937 | 0.01 | 0.3917 | 0.1338 | 0.1158 | 0.2863 | 0.027 | 0.0445 | 0.0279 | 0.0277 | 0.0012 | 0.0029 | 0.0052 |
Nuclear Periphery | 0.0194 | 0.0056 | 0.008 | 0.0021 | 0.003 | 0.0041 | 0.0147 | 0.0016 | 0.0033 | 0.0038 | 0.0015 | 0.0036 | 0.0196 | 0.0059 | 0.0049 | 0.0035 | 0.0082 | 0.0153 |
Nucleolus | 0.0168 | 0.003 | 0.0017 | 0.0412 | 0.0014 | 0.0009 | 0.0008 | 0.0007 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0017 | 0.0006 | 0.0002 | 0.0002 | 0.0006 | 0.0004 |
Nucleus | 0.0139 | 0.0042 | 0.0041 | 0.0077 | 0.0053 | 0.0018 | 0.0023 | 0.0004 | 0.001 | 0.001 | 0.0035 | 0.0012 | 0.0079 | 0.0015 | 0.0015 | 0.0012 | 0.0022 | 0.0034 |
Peroxisomes | 0.0187 | 0.0079 | 0.0106 | 0.0056 | 0.0846 | 0.0067 | 0.0123 | 0.0003 | 0.0051 | 0.001 | 0.0026 | 0.01 | 0.0175 | 0.0028 | 0.0313 | 0.003 | 0.0016 | 0.0125 |
Punctate Nuclear | 0.0196 | 0.0056 | 0.0077 | 0.002 | 0.0032 | 0.0022 | 0.0056 | 0.0015 | 0.0011 | 0.0009 | 0.0041 | 0.0017 | 0.0191 | 0.003 | 0.0027 | 0.0028 | 0.0019 | 0.0158 |
Vacuole | 0.016 | 0.0169 | 0.0087 | 0.0058 | 0.0076 | 0.0107 | 0.0047 | 0.001 | 0.0071 | 0.013 | 0.0012 | 0.0033 | 0.0043 | 0.0153 | 0.0056 | 0.0067 | 0.022 | 0.0059 |
Vacuole Periphery | 0.01 | 0.0033 | 0.0021 | 0.0037 | 0.0051 | 0.0025 | 0.0056 | 0.0003 | 0.0015 | 0.0049 | 0.0002 | 0.0015 | 0.002 | 0.0018 | 0.0019 | 0.0022 | 0.0108 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0695 | 8.5277 | 6.328 | 13.9031 | 10.307 | 11.653 | 9.1014 | 11.3158 | 12.3198 | 16.4548 |
Translational Efficiency | 2.3726 | 2.238 | 2.2469 | 1.3756 | 1.8628 | 1.7324 | 2.0415 | 1.371 | 1.5577 | 1.2864 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
227 | 1211 | 266 | 1258 | 1088 | 2657 | 1786 | 3 | 1315 | 3868 | 2052 | 1261 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 733.98 | 797.53 | 1080.10 | 967.61 | 716.51 | 793.68 | 873.69 | 384.68 | 719.53 | 794.89 | 900.45 | 966.22 |
Standard Deviation | 87.30 | 101.34 | 115.10 | 146.64 | 99.75 | 436.28 | 112.83 | 3.46 | 97.93 | 366.02 | 132.68 | 149.19 |
Intensity Change Log 2 | — | 0.119798 | 0.557352 | 0.398685 | — | 0.147571 | 0.286135 | -0.897328 | — | 0.133584 | 0.429735 | -0.101136 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000978 | 0.001868 | 0.027525 | 0.015859 | 0.002084 | 0.002509 | 0.021593 | 0.000038 | 0.001893 | 0.002308 | 0.022362 | 0.015821 |
Bud Neck | 0.020989 | 0.007919 | 0.021064 | 0.022936 | 0.008509 | 0.013860 | 0.022174 | 0.000005 | 0.010663 | 0.012000 | 0.022030 | 0.022881 |
Bud Site | 0.004727 | 0.009276 | 0.078898 | 0.097809 | 0.006051 | 0.010304 | 0.041354 | 0.000009 | 0.005822 | 0.009982 | 0.046221 | 0.097577 |
Cell Periphery | 0.001631 | 0.000435 | 0.001496 | 0.001139 | 0.000748 | 0.000581 | 0.000862 | 0.000158 | 0.000901 | 0.000536 | 0.000944 | 0.001136 |
Cytoplasm | 0.265031* | 0.154064* | 0.146241* | 0.291171* | 0.162180* | 0.178020* | 0.282213* | 0.000017 | 0.179934* | 0.170520* | 0.264587* | 0.290478* |
Cytoplasmic Foci | 0.223071* | 0.274805* | 0.101510 | 0.104590 | 0.274895* | 0.226220* | 0.158367 | 0.000004 | 0.265949* | 0.241431* | 0.150996 | 0.104341 |
Eisosomes | 0.002663 | 0.000822 | 0.000409 | 0.000732 | 0.001814 | 0.002195 | 0.001313 | 0.001382 | 0.001961 | 0.001765 | 0.001195 | 0.000733 |
Endoplasmic Reticulum | 0.081782 | 0.107828* | 0.095216 | 0.035395 | 0.069739 | 0.066678 | 0.090043 | 0.000000 | 0.071817 | 0.079561 | 0.090713 | 0.035311 |
Endosome | 0.045257 | 0.047390 | 0.042150 | 0.021738 | 0.051684 | 0.053184 | 0.026362 | 0.000000 | 0.050574 | 0.051370 | 0.028408 | 0.021686 |
Golgi | 0.030427 | 0.073553 | 0.002977 | 0.006752 | 0.082987 | 0.087653 | 0.014674 | 0.000003 | 0.073914 | 0.083239 | 0.013157 | 0.006736 |
Lipid Particles | 0.146843* | 0.202754* | 0.063911 | 0.031470 | 0.220732* | 0.195761* | 0.095609 | 0.000012 | 0.207977* | 0.197951* | 0.091500 | 0.031396 |
Mitochondria | 0.015125 | 0.021590 | 0.000307 | 0.006332 | 0.020629 | 0.047757 | 0.003654 | 0.000007 | 0.019679 | 0.039564 | 0.003220 | 0.006317 |
Mitotic Spindle | 0.000113 | 0.003892 | 0.021753 | 0.040243 | 0.000794 | 0.002615 | 0.008951 | 0.000126 | 0.000676 | 0.003014 | 0.010610 | 0.040147 |
None | 0.000439 | 0.001225 | 0.000274 | 0.000903 | 0.001075 | 0.002173 | 0.000660 | 0.997804* | 0.000965 | 0.001876 | 0.000610 | 0.003274 |
Nuclear Periphery | 0.002636 | 0.002164 | 0.004312 | 0.002065 | 0.002685 | 0.001396 | 0.001871 | 0.000000 | 0.002676 | 0.001637 | 0.002187 | 0.002060 |
Nuclear Periphery Foci | 0.003093 | 0.007961 | 0.087925 | 0.017652 | 0.005061 | 0.002249 | 0.022031 | 0.000000 | 0.004721 | 0.004037 | 0.030573 | 0.017610 |
Nucleolus | 0.000503 | 0.000648 | 0.000366 | 0.000645 | 0.001123 | 0.001107 | 0.000677 | 0.000001 | 0.001016 | 0.000963 | 0.000637 | 0.000644 |
Nucleus | 0.036443 | 0.026378 | 0.055640 | 0.055441 | 0.023996 | 0.026712 | 0.092277 | 0.000003 | 0.026145 | 0.026607 | 0.087528 | 0.055309 |
Peroxisomes | 0.018149 | 0.012665 | 0.014519 | 0.011914 | 0.020986 | 0.022195 | 0.018612 | 0.000430 | 0.020496 | 0.019211 | 0.018082 | 0.011887 |
Vacuole | 0.095056 | 0.037484 | 0.232674* | 0.228530* | 0.035779 | 0.048469 | 0.095981 | 0.000000 | 0.046012 | 0.045030 | 0.113700 | 0.227986* |
Vacuole Periphery | 0.005045 | 0.005280 | 0.000832 | 0.006684 | 0.006450 | 0.008363 | 0.000725 | 0.000000 | 0.006207 | 0.007398 | 0.000739 | 0.006668 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.64 | -5.22 | -8.03 | -7.45 | 2.61 | NaN | NaN | NaN | NaN | NaN | -1.40 | -12.18 | -7.41 | -7.17 | 4.14 |
Bud Neck | 3.01 | 0.03 | 0.05 | -8.03 | 0.03 | NaN | NaN | NaN | NaN | NaN | -1.15 | -6.84 | -5.85 | -5.73 | 0.48 |
Bud Site | -2.44 | -8.31 | -15.39 | -14.28 | -0.66 | NaN | NaN | NaN | NaN | NaN | -3.66 | -14.73 | -15.36 | -14.57 | -6.70 |
Cell Periphery | 3.27 | 0.50 | 1.87 | -4.97 | 3.42 | NaN | NaN | NaN | NaN | NaN | 2.80 | -0.08 | -0.20 | -4.29 | -0.21 |
Cytoplasm | 7.68 | 6.59 | -1.16 | -14.49 | -9.82 | NaN | NaN | NaN | NaN | NaN | 1.57 | -11.45 | -11.36 | -13.97 | -2.04 |
Cytoplasmic Foci | -3.94 | 9.45 | 10.33 | 27.06 | -0.17 | NaN | NaN | NaN | NaN | NaN | 4.08 | 18.76 | 26.89 | 31.75 | 10.85 |
Eisosomes | 2.76 | 3.42 | 3.14 | 2.48 | -2.81 | NaN | NaN | NaN | NaN | NaN | 0.92 | 3.76 | 7.10 | 10.40 | 4.57 |
Endoplasmic Reticulum | -2.17 | -0.48 | 4.83 | 13.03 | 7.11 | NaN | NaN | NaN | NaN | NaN | -1.62 | -2.86 | 9.07 | 14.25 | 13.50 |
Endosome | -0.40 | 0.93 | 5.09 | 12.88 | 7.63 | NaN | NaN | NaN | NaN | NaN | -0.33 | 9.29 | 13.09 | 20.71 | 5.98 |
Golgi | -7.79 | 6.15 | 5.62 | 21.23 | -2.79 | NaN | NaN | NaN | NaN | NaN | -2.55 | 18.99 | 21.53 | 39.19 | 6.97 |
Lipid Particles | -4.39 | 7.15 | 10.58 | 26.96* | 5.47 | NaN | NaN | NaN | NaN | NaN | 1.48 | 17.65 | 28.77 | 43.17* | 16.90 |
Mitochondria | -1.46 | 3.55 | 2.46 | 9.37 | -4.69 | NaN | NaN | NaN | NaN | NaN | -9.65 | 10.57 | 8.44 | 21.16 | -1.53 |
Mitotic Spindle | -3.18 | -4.61 | -10.37 | -8.91 | -3.08 | NaN | NaN | NaN | NaN | NaN | -4.16 | -7.87 | -10.21 | -9.47 | -7.10 |
None | -3.63 | 0.98 | -2.89 | 1.11 | -4.10 | NaN | NaN | NaN | NaN | NaN | -2.75 | 2.80 | -1.70 | -1.02 | -1.99 |
Nuclear Periphery | 0.83 | -1.20 | 0.35 | -0.61 | 1.41 | NaN | NaN | NaN | NaN | NaN | 2.56 | 1.05 | 0.50 | -2.43 | -0.59 |
Nuclear Periphery Foci | -4.82 | -11.34 | -9.22 | -5.74 | 9.03 | NaN | NaN | NaN | NaN | NaN | 1.16 | -15.08 | -8.18 | -8.83 | 5.36 |
Nucleolus | -1.86 | 1.25 | -2.86 | -1.43 | -3.87 | NaN | NaN | NaN | NaN | NaN | 0.15 | 1.90 | 1.19 | 1.42 | -1.14 |
Nucleus | 3.07 | -3.75 | -5.34 | -10.46 | 0.60 | NaN | NaN | NaN | NaN | NaN | -0.29 | -20.59 | -10.76 | -11.27 | 8.64 |
Peroxisomes | 1.92 | 1.45 | 2.83 | 2.58 | 1.15 | NaN | NaN | NaN | NaN | NaN | 1.01 | 2.17 | 7.47 | 8.68 | 5.59 |
Vacuole | 5.88 | -10.06 | -15.76 | -32.25 | -1.85 | NaN | NaN | NaN | NaN | NaN | 0.36 | -18.70 | -30.49 | -31.67 | -19.34 |
Vacuole Periphery | -0.31 | 6.04 | 0.36 | 0.63 | -3.86 | NaN | NaN | NaN | NaN | NaN | -1.80 | 9.26 | 1.41 | 2.77 | -4.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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