Standard name
Human Ortholog
Description GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 0.98 0.99 0.99 0.95 0.94 0.94 0.85 0.79 0.73 0.86 0.98 0.99 0.99 0.96 0.94 0.96 0.87 0.88 0.87 0.74 0.76 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.07 0 0 0 0.1 0.11 0.06 0.31 0.31 0.44 0.18 0 0 0 0 0 0 0.05 0 0.06 0.14 0.11 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 1 1 2 1 1 0 0 0 0 1 0 1 2 0 1 1 2 3
Bud 0 0 1 1 0 3 3 2 4 9 7 1 2 0 2 1 0 0 0 0 0 3 3 7
Bud Neck 0 0 4 5 7 3 4 15 3 4 0 3 0 0 0 0 0 1 0 1 0 0 1 4
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 8 6 5 5 5 1 3 5 2 2 4 2 1 14 5 5 0 1 1 1 1 1
Cytoplasm 119 142 261 250 362 344 317 458 316 332 215 276 208 303 351 200 138 113 111 149 229 121 246 268
Endoplasmic Reticulum 2 4 0 0 0 1 2 0 0 1 3 4 2 0 5 6 2 5 0 0 1 5 12 9
Endosome 0 0 0 0 0 0 0 1 0 1 0 2 0 0 0 1 2 1 2 1 0 0 1 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 1 2
Mitochondria 8 11 1 1 2 35 37 27 116 130 131 57 2 1 2 4 5 0 6 3 17 22 35 28
Nucleus 0 0 0 1 1 0 2 2 4 2 1 0 0 0 0 2 1 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 1 0 2 2 3 2 4 0 0 0 0 0 0 0 0 0 0 2 1
Vac/Vac Membrane 0 0 1 1 0 2 0 3 4 5 2 6 0 0 0 2 6 2 1 6 5 5 11 16
Unique Cell Count 126 150 265 253 365 364 337 485 373 422 296 321 212 305 356 209 147 118 128 169 265 165 325 354
Labelled Cell Count 129 161 276 266 379 394 371 512 455 497 364 355 218 307 361 231 159 130 128 169 265 165 325 354


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.9 5.3 4.7 5.1 4.0 4.2 4.6 3.9 3.6 3.6 4.1 4.4 4.7 4.8 7.1 7.0 6.9 4.4 4.5 4.9
Std Deviation (1e-4) 0.7 0.6 1.0 1.3 1.1 1.0 1.0 1.0 1.4 0.9 1.0 1.0 0.7 1.3 1.0 1.4 1.3 1.5 1.1 1.6 1.8
Intensity Change (Log2) – – – -0.15 -0.06 -0.41 -0.32 -0.21 -0.44 -0.54 -0.57 -0.34 -0.26 -0.15 -0.14 0.42 0.41 0.39 -0.27 -0.23 -0.1

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1803 -0.4625 -0.7251 -0.1962 -0.5206 -0.4372 -0.6888 -0.5708 -0.5995 -0.8385 -0.9779 -0.7151 -0.5543 -0.0876 -0.6699 -0.0727 -0.8377 -0.4863
Actin 0.0521 0.0006 0.0114 0.0145 0.1026 0.0013 0.0173 0.0063 0.0321 0.0041 0.005 0.0054 0.0326 0.0004 0.0149 0.1307 0.0047 0.0073
Bud 0.0009 0.0005 0.0003 0.0005 0.0017 0.0001 0.0007 0.0028 0.0057 0.0159 0.0024 0.0017 0.0016 0.0126 0.0006 0.0062 0.0005 0.001
Bud Neck 0.0033 0.0015 0.0023 0.0005 0.0053 0.0003 0.0008 0.0013 0.004 0.0005 0.0012 0.0142 0.0044 0.0005 0.0037 0.0099 0.0012 0.0007
Bud Periphery 0.0012 0.0004 0.0004 0.0008 0.0033 0.0001 0.0013 0.0039 0.0103 0.0283 0.0026 0.0026 0.0027 0.0178 0.0017 0.0125 0.001 0.0013
Bud Site 0.0048 0.0123 0.0128 0.0033 0.0188 0 0.0021 0.0115 0.01 0.0009 0.0043 0.008 0.0071 0.0131 0.0061 0.0386 0.002 0.0002
Cell Periphery 0.0004 0.0005 0.0004 0.0005 0.001 0.0001 0.0003 0.0004 0.0003 0.0002 0.0001 0.0004 0.0005 0.0004 0.0006 0.0007 0.0002 0.0001
Cytoplasm 0.1294 0.2664 0.1861 0.2521 0.236 0.3694 0.2965 0.3598 0.3655 0.4439 0.4014 0.4319 0.3364 0.4794 0.3644 0.2368 0.4366 0.508
Cytoplasmic Foci 0.0399 0.0122 0.0369 0.0186 0.0136 0.0285 0.0269 0.0084 0.0222 0.0543 0.0317 0.027 0.0262 0.0087 0.0069 0.0132 0.0225 0.0157
Eisosomes 0.0016 0.0002 0.0008 0.0008 0.0015 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0003 0.0012 0.0016 0.0002
Endoplasmic Reticulum 0.0084 0.0034 0.0021 0.0163 0.0035 0.0019 0.004 0.0025 0.0038 0.0021 0.0021 0.0024 0.0044 0.0018 0.0025 0.0039 0.001 0.0035
Endosome 0.0353 0.0052 0.0068 0.0528 0.0085 0.0025 0.0308 0.003 0.0234 0.0223 0.0238 0.0169 0.0147 0.0018 0.0028 0.0093 0.0074 0.0176
Golgi 0.008 0.0005 0.0008 0.0078 0.0072 0.0003 0.0063 0.0005 0.0093 0.0183 0.0172 0.0062 0.0076 0.0002 0.0005 0.0057 0.0028 0.0045
Lipid Particles 0.0204 0.0008 0.0018 0.0067 0.0066 0.0011 0.0104 0.0004 0.0061 0.0078 0.007 0.0032 0.0267 0.0005 0.0008 0.0023 0.0187 0.0045
Mitochondria 0.0038 0.0004 0.0005 0.0069 0.0058 0.0004 0.006 0.0008 0.0102 0.0036 0.0066 0.0089 0.01 0.0003 0.0006 0.0051 0.0038 0.004
None 0.6018 0.6893 0.7282 0.5588 0.5594 0.5892 0.5157 0.5914 0.4771 0.3658 0.4761 0.4604 0.4824 0.4572 0.5891 0.5037 0.4749 0.4175
Nuclear Periphery 0.037 0.0011 0.0006 0.0278 0.0094 0.0004 0.03 0.0012 0.0023 0.0008 0.0017 0.0009 0.0041 0.0003 0.0007 0.0053 0.0005 0.0053
Nucleolus 0.0019 0.0002 0.0007 0.0007 0.0005 0.0001 0.0027 0.0002 0.0006 0.0002 0.0007 0.0004 0.0012 0.0005 0.0002 0.0007 0.0019 0.0002
Nucleus 0.0179 0.001 0.0009 0.0088 0.0011 0.0003 0.0143 0.0014 0.0018 0.001 0.0019 0.0022 0.0047 0.0011 0.001 0.0022 0.0009 0.001
Peroxisomes 0.0073 0.0002 0.0016 0.0026 0.0075 0.0028 0.012 0.0007 0.0083 0.0249 0.0035 0.0026 0.0251 0.0002 0.0005 0.0067 0.0119 0.0037
Punctate Nuclear 0.0182 0.0006 0.0023 0.0137 0.0042 0.0005 0.0083 0.0007 0.0024 0.0009 0.0074 0.0016 0.0028 0.0006 0.0005 0.0015 0.0037 0.0018
Vacuole 0.004 0.0025 0.0019 0.0035 0.0016 0.0007 0.0089 0.0024 0.0035 0.0034 0.0026 0.0024 0.0039 0.0022 0.0015 0.0028 0.0018 0.0013
Vacuole Periphery 0.0023 0.0003 0.0002 0.002 0.0009 0.0001 0.0045 0.0003 0.001 0.0008 0.0007 0.0006 0.0006 0.0002 0.0002 0.0011 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0801 8.7912 8.4805 12.2589 8.2879 6.3887 12.111 13.1986 9.708 12.9925
Translational Efficiency 0.9313 1.0267 0.9579 0.6608 1.0045 1.3764 0.8239 0.6777 0.9226 0.716

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1713 279 300 904 1419 1840 188 1877 3132 2119 488 2781

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 681.31 769.07 1008.72 922.49 657.13 777.76 986.47 937.56 670.35 776.62 1000.15 932.66
Standard Deviation 74.49 113.36 109.30 134.74 74.88 104.27 121.80 122.67 75.63 105.55 114.79 126.92
Intensity Change Log 2 — 0.174804 0.566142 0.437222 — 0.243146 0.586096 0.512732 — 0.208762 0.575974 0.474789

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000182 0.000693 0.000622 0.000620 0.000093 0.000245 0.000329 0.000754 0.000142 0.000304 0.000509 0.000710
Bud Neck 0.024741 0.029876 0.003574 0.004295 0.015023 0.028409 0.001372 0.001772 0.020338 0.028602 0.002726 0.002592
Bud Site 0.009595 0.023977 0.012895 0.046934 0.003946 0.015629 0.004667 0.019236 0.007035 0.016728 0.009725 0.028240
Cell Periphery 0.000140 0.000107 0.000230 0.000096 0.000193 0.000101 0.000213 0.000060 0.000164 0.000102 0.000224 0.000072
Cytoplasm 0.541588 0.401008 0.368955 0.590820 0.460013 0.489584 0.505859 0.728752 0.504629 0.477922 0.421697 0.683915
Cytoplasmic Foci 0.192230 0.283756 0.027975 0.057411 0.270608 0.200199 0.044346 0.045114 0.227740 0.211201 0.034282 0.049111
Eisosomes 0.000189 0.000122 0.000081 0.000026 0.000221 0.000114 0.000094 0.000019 0.000204 0.000115 0.000086 0.000021
Endoplasmic Reticulum 0.001047 0.000779 0.007835 0.001422 0.001906 0.000525 0.003256 0.000323 0.001436 0.000558 0.006071 0.000680
Endosome 0.015491 0.029989 0.007988 0.013386 0.018715 0.026206 0.010739 0.003682 0.016951 0.026704 0.009048 0.006837
Golgi 0.004582 0.006653 0.000183 0.005341 0.004349 0.004373 0.000145 0.001764 0.004477 0.004673 0.000168 0.002927
Lipid Particles 0.005686 0.003702 0.003538 0.000843 0.013505 0.004085 0.003493 0.000366 0.009228 0.004034 0.003521 0.000521
Mitochondria 0.002935 0.006924 0.000389 0.001967 0.006448 0.005419 0.000105 0.000914 0.004526 0.005618 0.000280 0.001256
Mitotic Spindle 0.000402 0.005495 0.001947 0.049845 0.001402 0.008458 0.000510 0.017303 0.000855 0.008068 0.001394 0.027881
None 0.004209 0.000862 0.002569 0.002429 0.003677 0.001726 0.001149 0.001559 0.003968 0.001612 0.002022 0.001841
Nuclear Periphery 0.000166 0.000829 0.002366 0.001435 0.000526 0.000449 0.001046 0.000181 0.000329 0.000499 0.001857 0.000589
Nuclear Periphery Foci 0.000346 0.001151 0.007650 0.004813 0.000284 0.000577 0.009919 0.000796 0.000318 0.000653 0.008524 0.002102
Nucleolus 0.001134 0.001257 0.000416 0.000167 0.000809 0.001261 0.000218 0.000111 0.000987 0.001261 0.000340 0.000129
Nucleus 0.089232 0.073036 0.356169 0.030287 0.120520 0.094697 0.142536 0.033720 0.103407 0.091845 0.273868 0.032604
Peroxisomes 0.003509 0.023616 0.000492 0.002178 0.005333 0.006861 0.000364 0.001406 0.004335 0.009067 0.000443 0.001657
Vacuole 0.100957 0.104443 0.193801 0.181031 0.070489 0.107546 0.269430 0.140193 0.087153 0.107138 0.222937 0.153468
Vacuole Periphery 0.001639 0.001726 0.000325 0.004656 0.001939 0.003535 0.000209 0.001977 0.001775 0.003297 0.000280 0.002848

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.22 -9.49 -3.10 0.91 3.11 -3.73 -7.01 -2.43 -1.21 -0.20 -3.38 -11.44 -3.06 -1.21 2.06
Bud Neck -0.89 13.73 14.62 6.40 2.59 -6.59 11.20 11.23 16.24 0.03 -4.47 17.28 18.79 17.29 4.09
Bud Site -4.33 0.19 -5.05 -0.53 -3.93 -8.73 -1.72 -5.81 2.19 -4.98 -7.69 -0.26 -6.61 0.26 -3.96
Cell Periphery 2.86 -5.58 8.40 3.12 9.65 4.49 -0.20 7.33 10.79 8.54 5.89 -3.85 10.89 10.36 13.42
Cytoplasm 8.15 15.96 0.53 -7.02 -13.53 -2.98 2.13 -20.79 -17.98 -11.01 3.61 12.44 -15.43 -16.64 -20.59
Cytoplasmic Foci -6.22 35.38 30.19 17.72 -7.37 10.00 36.56 40.73 33.56 -0.67 3.32 50.57 50.48 37.31 -5.78
Eisosomes 4.40 7.00 12.90 9.90 11.42 8.27 9.18 18.60 12.79 10.55 8.44 11.30 21.57 14.20 16.15
Endoplasmic Reticulum 0.69 -11.70 0.93 -0.12 11.93 3.96 -3.57 4.64 4.37 7.34 4.59 -11.51 5.08 1.02 13.93
Endosome -3.32 7.24 7.24 5.64 1.66 -4.07 6.25 13.56 17.74 6.64 -6.48 9.66 16.83 17.51 7.66
Golgi -0.89 15.19 3.53 2.13 -2.67 -0.01 9.21 6.68 8.43 -2.23 -0.29 16.52 8.50 7.28 -3.43
Lipid Particles 1.24 4.45 14.04 2.41 9.51 9.67 10.52 14.41 10.51 8.38 8.93 11.20 18.94 10.10 13.27
Mitochondria -1.18 9.81 6.18 1.86 -1.50 0.85 6.09 5.67 8.04 -1.68 -1.31 8.54 7.68 7.48 -1.57
Mitotic Spindle -2.68 -3.68 -6.34 -4.42 -5.80 -4.18 0.97 -5.24 -0.83 -6.03 -5.14 -2.10 -8.45 -3.34 -7.91
None 6.69 4.01 4.39 -2.70 0.48 2.23 2.87 2.45 0.70 -1.19 4.77 4.53 4.76 -0.02 0.32
Nuclear Periphery -1.06 -18.50 -2.77 -0.09 7.56 0.76 -6.93 2.51 1.09 9.40 -1.58 -18.22 -2.26 -0.22 13.76
Nuclear Periphery Foci -1.47 -10.45 -4.19 -1.72 5.86 -1.02 -9.15 -8.93 -0.72 8.65 -1.36 -13.88 -5.63 -2.43 11.49
Nucleolus -0.47 4.94 6.80 2.11 6.34 -2.62 7.18 8.09 6.34 2.85 -1.87 7.35 9.67 6.62 7.17
Nucleus 1.94 -16.74 14.98 4.25 19.81 4.61 -1.81 18.00 13.13 7.94 2.56 -14.28 23.49 14.21 19.51
Peroxisomes -4.35 8.43 5.76 4.93 -2.63 -2.08 8.93 7.70 11.37 -1.65 -5.81 12.20 9.72 10.91 -2.46
Vacuole -1.37 -11.57 -20.61 -14.69 -2.70 -8.93 -14.25 -26.76 -19.62 6.33 -5.95 -18.05 -32.29 -25.71 3.62
Vacuole Periphery -0.02 7.73 -0.17 -0.14 -2.13 -3.29 10.05 2.12 4.05 -1.36 -3.66 12.30 1.53 3.82 -2.23
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (71%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ski7

Ski7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available