Standard name
Human Ortholog
Description GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 0.98 0.99 0.99 0.95 0.94 0.94 0.85 0.79 0.73 0.86 0.98 0.99 0.99 0.96 0.94 0.96 0.87 0.88 0.87 0.74 0.76 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.07 0 0 0 0.1 0.11 0.06 0.31 0.31 0.44 0.18 0 0 0 0 0 0 0.05 0 0.06 0.14 0.11 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 1 1 2 1 1 0 0 0 0 1 0 1 2 0 1 1 2 3
Bud 0 0 1 1 0 3 3 2 4 9 7 1 2 0 2 1 0 0 0 0 0 3 3 7
Bud Neck 0 0 4 5 7 3 4 15 3 4 0 3 0 0 0 0 0 1 0 1 0 0 1 4
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 8 6 5 5 5 1 3 5 2 2 4 2 1 14 5 5 0 1 1 1 1 1
Cytoplasm 119 142 261 250 362 344 317 458 316 332 215 276 208 303 351 200 138 113 111 149 229 121 246 268
Endoplasmic Reticulum 2 4 0 0 0 1 2 0 0 1 3 4 2 0 5 6 2 5 0 0 1 5 12 9
Endosome 0 0 0 0 0 0 0 1 0 1 0 2 0 0 0 1 2 1 2 1 0 0 1 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 1 2
Mitochondria 8 11 1 1 2 35 37 27 116 130 131 57 2 1 2 4 5 0 6 3 17 22 35 28
Nucleus 0 0 0 1 1 0 2 2 4 2 1 0 0 0 0 2 1 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 1 0 2 2 3 2 4 0 0 0 0 0 0 0 0 0 0 2 1
Vac/Vac Membrane 0 0 1 1 0 2 0 3 4 5 2 6 0 0 0 2 6 2 1 6 5 5 11 16
Unique Cell Count 126 150 265 253 365 364 337 485 373 422 296 321 212 305 356 209 147 118 128 169 265 165 325 354
Labelled Cell Count 129 161 276 266 379 394 371 512 455 497 364 355 218 307 361 231 159 130 128 169 265 165 325 354


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.9 5.3 4.7 5.1 4.0 4.2 4.6 3.9 3.6 3.6 4.1 4.4 4.7 4.8 7.1 7.0 6.9 4.4 4.5 4.9
Std Deviation (1e-4) 0.7 0.6 1.0 1.3 1.1 1.0 1.0 1.0 1.4 0.9 1.0 1.0 0.7 1.3 1.0 1.4 1.3 1.5 1.1 1.6 1.8
Intensity Change (Log2) – – – -0.15 -0.06 -0.41 -0.32 -0.21 -0.44 -0.54 -0.57 -0.34 -0.26 -0.15 -0.14 0.42 0.41 0.39 -0.27 -0.23 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1803 -0.4625 -0.7251 -0.1962 -0.5206 -0.4372 -0.6888 -0.5708 -0.5995 -0.8385 -0.9779 -0.7151 -0.5543 -0.0876 -0.6699 -0.0727 -0.8377 -0.4863
Actin 0.0521 0.0006 0.0114 0.0145 0.1026 0.0013 0.0173 0.0063 0.0321 0.0041 0.005 0.0054 0.0326 0.0004 0.0149 0.1307 0.0047 0.0073
Bud 0.0009 0.0005 0.0003 0.0005 0.0017 0.0001 0.0007 0.0028 0.0057 0.0159 0.0024 0.0017 0.0016 0.0126 0.0006 0.0062 0.0005 0.001
Bud Neck 0.0033 0.0015 0.0023 0.0005 0.0053 0.0003 0.0008 0.0013 0.004 0.0005 0.0012 0.0142 0.0044 0.0005 0.0037 0.0099 0.0012 0.0007
Bud Periphery 0.0012 0.0004 0.0004 0.0008 0.0033 0.0001 0.0013 0.0039 0.0103 0.0283 0.0026 0.0026 0.0027 0.0178 0.0017 0.0125 0.001 0.0013
Bud Site 0.0048 0.0123 0.0128 0.0033 0.0188 0 0.0021 0.0115 0.01 0.0009 0.0043 0.008 0.0071 0.0131 0.0061 0.0386 0.002 0.0002
Cell Periphery 0.0004 0.0005 0.0004 0.0005 0.001 0.0001 0.0003 0.0004 0.0003 0.0002 0.0001 0.0004 0.0005 0.0004 0.0006 0.0007 0.0002 0.0001
Cytoplasm 0.1294 0.2664 0.1861 0.2521 0.236 0.3694 0.2965 0.3598 0.3655 0.4439 0.4014 0.4319 0.3364 0.4794 0.3644 0.2368 0.4366 0.508
Cytoplasmic Foci 0.0399 0.0122 0.0369 0.0186 0.0136 0.0285 0.0269 0.0084 0.0222 0.0543 0.0317 0.027 0.0262 0.0087 0.0069 0.0132 0.0225 0.0157
Eisosomes 0.0016 0.0002 0.0008 0.0008 0.0015 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0003 0.0012 0.0016 0.0002
Endoplasmic Reticulum 0.0084 0.0034 0.0021 0.0163 0.0035 0.0019 0.004 0.0025 0.0038 0.0021 0.0021 0.0024 0.0044 0.0018 0.0025 0.0039 0.001 0.0035
Endosome 0.0353 0.0052 0.0068 0.0528 0.0085 0.0025 0.0308 0.003 0.0234 0.0223 0.0238 0.0169 0.0147 0.0018 0.0028 0.0093 0.0074 0.0176
Golgi 0.008 0.0005 0.0008 0.0078 0.0072 0.0003 0.0063 0.0005 0.0093 0.0183 0.0172 0.0062 0.0076 0.0002 0.0005 0.0057 0.0028 0.0045
Lipid Particles 0.0204 0.0008 0.0018 0.0067 0.0066 0.0011 0.0104 0.0004 0.0061 0.0078 0.007 0.0032 0.0267 0.0005 0.0008 0.0023 0.0187 0.0045
Mitochondria 0.0038 0.0004 0.0005 0.0069 0.0058 0.0004 0.006 0.0008 0.0102 0.0036 0.0066 0.0089 0.01 0.0003 0.0006 0.0051 0.0038 0.004
None 0.6018 0.6893 0.7282 0.5588 0.5594 0.5892 0.5157 0.5914 0.4771 0.3658 0.4761 0.4604 0.4824 0.4572 0.5891 0.5037 0.4749 0.4175
Nuclear Periphery 0.037 0.0011 0.0006 0.0278 0.0094 0.0004 0.03 0.0012 0.0023 0.0008 0.0017 0.0009 0.0041 0.0003 0.0007 0.0053 0.0005 0.0053
Nucleolus 0.0019 0.0002 0.0007 0.0007 0.0005 0.0001 0.0027 0.0002 0.0006 0.0002 0.0007 0.0004 0.0012 0.0005 0.0002 0.0007 0.0019 0.0002
Nucleus 0.0179 0.001 0.0009 0.0088 0.0011 0.0003 0.0143 0.0014 0.0018 0.001 0.0019 0.0022 0.0047 0.0011 0.001 0.0022 0.0009 0.001
Peroxisomes 0.0073 0.0002 0.0016 0.0026 0.0075 0.0028 0.012 0.0007 0.0083 0.0249 0.0035 0.0026 0.0251 0.0002 0.0005 0.0067 0.0119 0.0037
Punctate Nuclear 0.0182 0.0006 0.0023 0.0137 0.0042 0.0005 0.0083 0.0007 0.0024 0.0009 0.0074 0.0016 0.0028 0.0006 0.0005 0.0015 0.0037 0.0018
Vacuole 0.004 0.0025 0.0019 0.0035 0.0016 0.0007 0.0089 0.0024 0.0035 0.0034 0.0026 0.0024 0.0039 0.0022 0.0015 0.0028 0.0018 0.0013
Vacuole Periphery 0.0023 0.0003 0.0002 0.002 0.0009 0.0001 0.0045 0.0003 0.001 0.0008 0.0007 0.0006 0.0006 0.0002 0.0002 0.0011 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0801 8.7912 8.4805 12.2589 8.2879 6.3887 12.111 13.1986 9.708 12.9925
Translational Efficiency 0.9313 1.0267 0.9579 0.6608 1.0045 1.3764 0.8239 0.6777 0.9226 0.716

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1713 279 300 904 1419 1840 188 1877 3132 2119 488 2781

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 681.31 769.07 1008.72 922.49 657.13 777.76 986.47 937.56 670.35 776.62 1000.15 932.66
Standard Deviation 74.49 113.36 109.30 134.74 74.88 104.27 121.80 122.67 75.63 105.55 114.79 126.92
Intensity Change Log 2 — 0.174804 0.566142 0.437222 — 0.243146 0.586096 0.512732 — 0.208762 0.575974 0.474789

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000182 0.000693 0.000622 0.000620 0.000093 0.000245 0.000329 0.000754 0.000142 0.000304 0.000509 0.000710
Bud Neck 0.024741 0.029876 0.003574 0.004295 0.015023 0.028409 0.001372 0.001772 0.020338 0.028602 0.002726 0.002592
Bud Site 0.009595 0.023977 0.012895 0.046934 0.003946 0.015629 0.004667 0.019236 0.007035 0.016728 0.009725 0.028240
Cell Periphery 0.000140 0.000107 0.000230 0.000096 0.000193 0.000101 0.000213 0.000060 0.000164 0.000102 0.000224 0.000072
Cytoplasm 0.541588* 0.401008* 0.368955* 0.590820* 0.460013* 0.489584* 0.505859* 0.728752* 0.504629* 0.477922* 0.421697* 0.683915*
Cytoplasmic Foci 0.192230* 0.283756* 0.027975 0.057411 0.270608* 0.200199* 0.044346 0.045114 0.227740* 0.211201* 0.034282 0.049111
Eisosomes 0.000189 0.000122 0.000081 0.000026 0.000221 0.000114 0.000094 0.000019 0.000204 0.000115 0.000086 0.000021
Endoplasmic Reticulum 0.001047 0.000779 0.007835 0.001422 0.001906 0.000525 0.003256 0.000323 0.001436 0.000558 0.006071 0.000680
Endosome 0.015491 0.029989 0.007988 0.013386 0.018715 0.026206 0.010739 0.003682 0.016951 0.026704 0.009048 0.006837
Golgi 0.004582 0.006653 0.000183 0.005341 0.004349 0.004373 0.000145 0.001764 0.004477 0.004673 0.000168 0.002927
Lipid Particles 0.005686 0.003702 0.003538 0.000843 0.013505 0.004085 0.003493 0.000366 0.009228 0.004034 0.003521 0.000521
Mitochondria 0.002935 0.006924 0.000389 0.001967 0.006448 0.005419 0.000105 0.000914 0.004526 0.005618 0.000280 0.001256
Mitotic Spindle 0.000402 0.005495 0.001947 0.049845 0.001402 0.008458 0.000510 0.017303 0.000855 0.008068 0.001394 0.027881
None 0.004209 0.000862 0.002569 0.002429 0.003677 0.001726 0.001149 0.001559 0.003968 0.001612 0.002022 0.001841
Nuclear Periphery 0.000166 0.000829 0.002366 0.001435 0.000526 0.000449 0.001046 0.000181 0.000329 0.000499 0.001857 0.000589
Nuclear Periphery Foci 0.000346 0.001151 0.007650 0.004813 0.000284 0.000577 0.009919 0.000796 0.000318 0.000653 0.008524 0.002102
Nucleolus 0.001134 0.001257 0.000416 0.000167 0.000809 0.001261 0.000218 0.000111 0.000987 0.001261 0.000340 0.000129
Nucleus 0.089232 0.073036 0.356169* 0.030287 0.120520 0.094697 0.142536* 0.033720 0.103407 0.091845 0.273868* 0.032604
Peroxisomes 0.003509 0.023616 0.000492 0.002178 0.005333 0.006861 0.000364 0.001406 0.004335 0.009067 0.000443 0.001657
Vacuole 0.100957 0.104443 0.193801 0.181031 0.070489 0.107546 0.269430* 0.140193 0.087153 0.107138 0.222937* 0.153468
Vacuole Periphery 0.001639 0.001726 0.000325 0.004656 0.001939 0.003535 0.000209 0.001977 0.001775 0.003297 0.000280 0.002848

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.22 -9.49 -3.10 0.91 3.11 -3.73 -7.01 -2.43 -1.21 -0.20 -3.38 -11.44 -3.06 -1.21 2.06
Bud Neck -0.89 13.73 14.62 6.40 2.59 -6.59 11.20 11.23 16.24 0.03 -4.47 17.28 18.79 17.29 4.09
Bud Site -4.33 0.19 -5.05 -0.53 -3.93 -8.73 -1.72 -5.81 2.19 -4.98 -7.69 -0.26 -6.61 0.26 -3.96
Cell Periphery 2.86 -5.58 8.40 3.12 9.65 4.49 -0.20 7.33 10.79 8.54 5.89 -3.85 10.89 10.36 13.42
Cytoplasm 8.15 15.96 0.53 -7.02 -13.53 -2.98 2.13 -20.79 -17.98 -11.01 3.61 12.44 -15.43 -16.64 -20.59
Cytoplasmic Foci -6.22 35.38 30.19 17.72 -7.37 10.00 36.56 40.73 33.56 -0.67 3.32 50.57 50.48 37.31 -5.78
Eisosomes 4.40 7.00 12.90 9.90 11.42 8.27 9.18 18.60 12.79 10.55 8.44 11.30 21.57 14.20 16.15
Endoplasmic Reticulum 0.69 -11.70 0.93 -0.12 11.93 3.96 -3.57 4.64 4.37 7.34 4.59 -11.51 5.08 1.02 13.93
Endosome -3.32 7.24 7.24 5.64 1.66 -4.07 6.25 13.56 17.74 6.64 -6.48 9.66 16.83 17.51 7.66
Golgi -0.89 15.19 3.53 2.13 -2.67 -0.01 9.21 6.68 8.43 -2.23 -0.29 16.52 8.50 7.28 -3.43
Lipid Particles 1.24 4.45 14.04 2.41 9.51 9.67 10.52 14.41 10.51 8.38 8.93 11.20 18.94 10.10 13.27
Mitochondria -1.18 9.81 6.18 1.86 -1.50 0.85 6.09 5.67 8.04 -1.68 -1.31 8.54 7.68 7.48 -1.57
Mitotic Spindle -2.68 -3.68 -6.34 -4.42 -5.80 -4.18 0.97 -5.24 -0.83 -6.03 -5.14 -2.10 -8.45 -3.34 -7.91
None 6.69 4.01 4.39 -2.70 0.48 2.23 2.87 2.45 0.70 -1.19 4.77 4.53 4.76 -0.02 0.32
Nuclear Periphery -1.06 -18.50 -2.77 -0.09 7.56 0.76 -6.93 2.51 1.09 9.40 -1.58 -18.22 -2.26 -0.22 13.76
Nuclear Periphery Foci -1.47 -10.45 -4.19 -1.72 5.86 -1.02 -9.15 -8.93 -0.72 8.65 -1.36 -13.88 -5.63 -2.43 11.49
Nucleolus -0.47 4.94 6.80 2.11 6.34 -2.62 7.18 8.09 6.34 2.85 -1.87 7.35 9.67 6.62 7.17
Nucleus 1.94 -16.74 14.98 4.25 19.81 4.61 -1.81 18.00 13.13 7.94 2.56 -14.28 23.49 14.21 19.51
Peroxisomes -4.35 8.43 5.76 4.93 -2.63 -2.08 8.93 7.70 11.37 -1.65 -5.81 12.20 9.72 10.91 -2.46
Vacuole -1.37 -11.57 -20.61 -14.69 -2.70 -8.93 -14.25 -26.76 -19.62 6.33 -5.95 -18.05 -32.29 -25.71 3.62
Vacuole Periphery -0.02 7.73 -0.17 -0.14 -2.13 -3.29 10.05 2.12 4.05 -1.36 -3.66 12.30 1.53 3.82 -2.23
Standard name
Human Ortholog
Description GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (71%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ski7

Ski7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available