Standard name
Human Ortholog
Description Gamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0
Cytoplasm 0.9 0.89 0.95 0.92 0.85 0.67 0.59 0.57 0.5 0.52 0.52 0.52 0.91 0.95 0.98 0.95 0.93 0.93 0.58 0.61 0.34 0.33 0.42 0.39
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.07 0.08 0.06 0.17 0.09 0.28 0.16 0.13 0.16
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.09 0.11 0.37 0.49 0.46 0.57 0.57 0.55 0.49 0 0 0 0.06 0 0 0.12 0.14 0.19 0.3 0.24 0.21
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.05 0 0 0.06 0.05 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 0 1 0 0 0 0 3 4 0 0 0 0 0 0 1 5 8 28 1 4 7
Bud 3 4 1 5 3 22 22 30 29 28 35 33 1 0 0 1 0 1 0 1 3 0 0 2
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 5 6 7 1 2 3
Bud Site 0 0 0 0 0 0 1 1 4 2 1 4 0 0 0 0 1 0 – – – – – –
Cell Periphery 6 2 4 7 8 9 4 7 11 13 10 19 3 1 1 6 7 6 4 5 17 4 8 6
Cytoplasm 224 270 384 341 474 394 432 480 330 438 312 379 78 40 55 156 204 157 172 216 151 21 65 66
Endoplasmic Reticulum 13 5 8 0 1 12 4 3 18 11 21 12 7 1 1 12 18 10 51 30 124 10 20 28
Endosome 1 3 4 4 22 26 18 33 8 18 11 36 0 0 0 1 2 3 3 9 8 0 2 2
Golgi 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 2 0 1 1 0 0 0
Mitochondria 5 18 8 33 59 218 361 388 376 480 330 361 0 0 0 10 9 4 36 50 85 19 38 36
Nucleus 1 2 1 2 1 2 4 11 4 3 6 2 2 0 0 0 1 1 0 1 0 0 0 0
Nuclear Periphery 0 9 0 0 1 0 3 5 0 8 0 1 0 1 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 3 1 1 1 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 5 14 4 0 2 1 1 2 3 0 0 0 0 2 0 0 0 0 0 0 0
SpindlePole 0 1 3 2 3 4 3 11 3 10 4 13 0 0 0 0 2 1 1 3 1 0 2 0
Vac/Vac Membrane 1 1 3 5 52 31 24 34 37 46 18 40 0 0 1 1 6 1 13 14 5 2 6 11
Unique Cell Count 249 302 403 371 559 592 737 845 659 847 605 735 86 42 56 164 219 168 299 358 444 65 158 170
Labelled Cell Count 256 316 418 405 639 722 876 1008 823 1063 755 905 91 43 58 188 256 188 299 358 444 65 158 170


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.0 4.3 4.5 4.8 4.0 3.9 4.3 4.0 4.0 3.9 4.1 5.3 5.5 5.8 5.3 5.4 5.2 5.1 5.2 5.3
Std Deviation (1e-4) 0.4 0.6 1.1 1.6 2.0 1.3 1.4 1.2 1.4 1.1 1.0 1.0 1.0 0.9 2.0 1.5 1.1 0.8 0.6 1.4 1.1
Intensity Change (Log2) – – – 0.08 0.15 -0.12 -0.13 0.01 -0.12 -0.12 -0.13 -0.06 0.31 0.36 0.43 0.3 0.32 0.27 0.25 0.29 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4913 0.6551 0.6634 0.5315 0.5092 0.6648 2.2328 2.7581 2.5708 2.7843 2.413 2.5373 1.6415 1.8109 1.9549 2.0122 1.5838 1.8826
Actin 0.0054 0.0066 0.0118 0.0024 0.0039 0.0059 0.0206 0.0125 0.0136 0.0129 0.0146 0.0068 0.01 0.0066 0.0064 0.0257 0.0153 0.0024
Bud 0.0003 0.0004 0.0006 0.0001 0.0002 0.0001 0.0009 0.0005 0.0006 0.0017 0.0006 0.0003 0.0003 0.0005 0.0011 0.0004 0.0004 0.0004
Bud Neck 0.0007 0.0015 0.0017 0.0013 0.0157 0.0037 0.0057 0.0011 0.0029 0.0073 0.0055 0.0073 0.0012 0.0009 0.0018 0.0063 0.0042 0.0046
Bud Periphery 0.0005 0.0007 0.0012 0.0005 0.0006 0.0005 0.0024 0.0007 0.0016 0.0088 0.0011 0.0007 0.0009 0.0011 0.0024 0.0007 0.0008 0.0006
Bud Site 0.0045 0.0343 0.0061 0.0004 0.0012 0.0004 0.0103 0.0186 0.007 0.0033 0.0033 0.0011 0.0016 0.022 0.0083 0.0026 0.0012 0.0038
Cell Periphery 0.0044 0.0045 0.0063 0.0026 0.0046 0.0033 0.0051 0.0038 0.0045 0.0065 0.0029 0.0019 0.0039 0.0083 0.0047 0.0031 0.0174 0.002
Cytoplasm 0.4048 0.432 0.4707 0.4676 0.4329 0.4674 0.3727 0.4419 0.4385 0.3886 0.3662 0.4585 0.3924 0.5023 0.5058 0.4529 0.5853 0.4777
Cytoplasmic Foci 0.0238 0.0501 0.0286 0.0111 0.0329 0.0286 0.0324 0.0344 0.0414 0.0176 0.0481 0.0374 0.0204 0.0149 0.0175 0.0234 0.0308 0.0265
Eisosomes 0.0007 0.0006 0.001 0.0002 0.0009 0.0005 0.0006 0.0003 0.0005 0.002 0.0005 0.0002 0.0005 0.0003 0.0005 0.0004 0.0005 0.0001
Endoplasmic Reticulum 0.2035 0.16 0.2276 0.3905 0.1934 0.2068 0.271 0.2072 0.2533 0.2621 0.2662 0.2598 0.341 0.2407 0.261 0.2459 0.1737 0.2878
Endosome 0.0226 0.055 0.0273 0.0163 0.0393 0.0338 0.0421 0.0561 0.0344 0.0571 0.0474 0.0411 0.0217 0.0217 0.0185 0.0275 0.0417 0.0406
Golgi 0.0039 0.0088 0.009 0.0022 0.0037 0.0083 0.0099 0.0072 0.0097 0.0068 0.0276 0.01 0.0041 0.0058 0.006 0.0083 0.0207 0.0076
Lipid Particles 0.014 0.0189 0.0126 0.0096 0.0173 0.0115 0.0201 0.0152 0.0157 0.0249 0.0182 0.0139 0.0118 0.0221 0.0126 0.007 0.019 0.0147
Mitochondria 0.0015 0.0043 0.0038 0.0013 0.0086 0.0068 0.0098 0.0039 0.0067 0.0082 0.0221 0.0109 0.0066 0.0023 0.0029 0.0018 0.0244 0.0108
None 0.293 0.193 0.174 0.0753 0.2217 0.2085 0.1558 0.1724 0.1378 0.0961 0.1394 0.1304 0.1529 0.1342 0.1367 0.1751 0.0473 0.0969
Nuclear Periphery 0.0071 0.0063 0.0052 0.0075 0.0055 0.0034 0.0087 0.0054 0.0067 0.0272 0.0049 0.0058 0.0078 0.008 0.0037 0.0082 0.003 0.0045
Nucleolus 0.0005 0.0011 0.0004 0.0002 0.0011 0.0003 0.0023 0.0009 0.0014 0.001 0.0007 0.0002 0.0004 0.0002 0.0002 0.0002 0.0001 0.0011
Nucleus 0.0018 0.0052 0.0021 0.0032 0.0047 0.0026 0.0043 0.0047 0.0036 0.0112 0.0024 0.003 0.0043 0.0018 0.0012 0.0025 0.0009 0.0023
Peroxisomes 0.0008 0.0021 0.001 0.0002 0.0006 0.0008 0.0105 0.0013 0.0058 0.0444 0.0169 0.0024 0.0057 0.0002 0.0038 0.001 0.0068 0.0028
Punctate Nuclear 0.0016 0.0039 0.0027 0.001 0.0048 0.0022 0.0064 0.0028 0.0079 0.0029 0.0067 0.0027 0.0065 0.0008 0.0012 0.003 0.0022 0.0023
Vacuole 0.0042 0.0098 0.0057 0.0058 0.005 0.0038 0.0075 0.0086 0.0055 0.0067 0.004 0.0048 0.0054 0.0049 0.0034 0.0035 0.0034 0.0094
Vacuole Periphery 0.0005 0.0009 0.0007 0.0005 0.0012 0.0006 0.0011 0.0008 0.0009 0.0026 0.0007 0.0008 0.0006 0.0004 0.0004 0.0006 0.0009 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 88.0209 81.4955 73.6799 84.9158 83.5543 71.2155 99.4483 96.2066 78.4151 100.6677
Translational Efficiency 1.0006 1.0229 1.0705 0.7646 0.8632 1.3504 0.9808 0.9345 1.1696 0.8793

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1602 1564 665 950 1409 2618 1949 1638 3011 4182 2614 2588

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 658.90 768.91 908.41 900.60 691.22 750.01 942.32 925.99 674.02 757.08 933.69 916.67
Standard Deviation 75.67 105.12 110.91 136.94 95.09 98.01 120.70 130.03 86.82 101.14 119.20 133.17
Intensity Change Log 2 — 0.222755 0.463284 0.450827 — 0.117765 0.447072 0.421852 — 0.169958 0.455007 0.436065

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000093 0.000251 0.000661 0.006569 0.000192 0.000675 0.001393 0.004819 0.000139 0.000517 0.001206 0.005461
Bud Neck 0.012654 0.008415 0.005852 0.010739 0.009915 0.024722 0.007533 0.010307 0.011372 0.018624 0.007106 0.010465
Bud Site 0.002439 0.005879 0.005275 0.048788 0.005343 0.019592 0.008023 0.034541 0.003798 0.014464 0.007324 0.039771
Cell Periphery 0.000203 0.000288 0.000698 0.001754 0.000389 0.000480 0.000732 0.000679 0.000290 0.000408 0.000723 0.001074
Cytoplasm 0.434010* 0.497953* 0.310744* 0.317828* 0.413834* 0.325284* 0.349507* 0.272283* 0.424568* 0.389859* 0.339646* 0.289002*
Cytoplasmic Foci 0.268166* 0.219848* 0.068051 0.089879 0.273371* 0.233548* 0.044420 0.079334 0.270602* 0.228425* 0.050431 0.083205
Eisosomes 0.000328 0.000245 0.000218 0.000726 0.000510 0.000558 0.000321 0.000409 0.000413 0.000441 0.000295 0.000526
Endoplasmic Reticulum 0.003970 0.010485 0.097254 0.106252* 0.016110 0.035629 0.166517* 0.256652* 0.009651 0.026226 0.148897* 0.201443*
Endosome 0.014375 0.011298 0.015899 0.015456 0.016075 0.030239 0.007446 0.023594 0.015170 0.023155 0.009596 0.020607
Golgi 0.003430 0.004581 0.000791 0.008356 0.008258 0.015301 0.001247 0.006477 0.005690 0.011292 0.001131 0.007167
Lipid Particles 0.032150 0.023073 0.038675 0.035242 0.048339 0.073911 0.028712 0.061492 0.039726 0.054899 0.031246 0.051856
Mitochondria 0.004631 0.005269 0.000873 0.002275 0.006048 0.025831 0.000912 0.002801 0.005294 0.018141 0.000902 0.002608
Mitotic Spindle 0.000312 0.001664 0.002666 0.010974 0.001535 0.004713 0.001470 0.006004 0.000885 0.003573 0.001775 0.007828
None 0.003745 0.004467 0.002616 0.004384 0.003545 0.004672 0.001075 0.003151 0.003651 0.004596 0.001467 0.003603
Nuclear Periphery 0.001300 0.000950 0.028011 0.005492 0.001854 0.002302 0.016059 0.009382 0.001559 0.001797 0.019100 0.007954
Nuclear Periphery Foci 0.000916 0.002602 0.052422 0.032461 0.001784 0.002132 0.043007 0.039562 0.001322 0.002308 0.045403 0.036956
Nucleolus 0.001356 0.000857 0.001414 0.001436 0.001220 0.001804 0.000364 0.000820 0.001293 0.001450 0.000631 0.001046
Nucleus 0.159504* 0.149026* 0.281548* 0.124024 0.142952* 0.102333 0.244048* 0.063848 0.151759* 0.119795 0.253588* 0.085938
Peroxisomes 0.004147 0.003962 0.001956 0.004223 0.005413 0.009663 0.001520 0.004104 0.004740 0.007531 0.001631 0.004147
Vacuole 0.051013 0.047639 0.083914 0.171859 0.041868 0.081521 0.075455 0.118052 0.046734 0.068850 0.077607 0.137803
Vacuole Periphery 0.001258 0.001248 0.000464 0.001283 0.001445 0.005088 0.000238 0.001688 0.001345 0.003651 0.000296 0.001540

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.27 -4.85 -4.86 -4.73 -4.33 -4.02 -7.53 -4.44 -3.91 -3.08 -4.99 -8.75 -6.49 -5.97 -4.94
Bud Neck 2.80 4.67 1.19 -1.67 -3.62 -9.42 1.68 -0.57 9.48 -2.48 -6.28 4.09 0.72 7.40 -3.75
Bud Site -3.37 -2.03 -10.03 -9.10 -8.97 -7.97 -2.47 -9.93 -4.52 -8.82 -9.38 -4.36 -14.42 -9.35 -12.43
Cell Periphery -3.78 -7.73 -10.54 -9.86 -6.32 -2.42 -6.40 -5.74 -3.76 1.59 -5.17 -10.60 -12.56 -10.35 -4.23
Cytoplasm -7.34 11.73 12.12 17.63 -0.20 11.23 8.64 15.89 6.75 8.65 5.95 14.56 20.91 16.08 7.03
Cytoplasmic Foci 6.95 31.58 28.02 20.71 -3.64 6.14 40.88 32.22 32.55 -10.60 9.01 54.91 43.48 38.36 -11.37
Eisosomes 3.06 3.73 -3.93 -5.05 -5.29 -0.71 2.76 1.65 3.92 -1.62 -0.77 3.06 -1.58 -1.22 -4.58
Endoplasmic Reticulum -5.40 -13.87 -13.99 -12.80 0.27 -7.70 -26.73 -27.75 -24.99 -8.15 -10.73 -31.15 -30.69 -27.27 -5.68
Endosome 2.81 -0.80 -0.20 -2.58 0.61 -9.94 8.47 -5.11 4.91 -15.10 -8.36 7.15 -5.05 2.99 -11.86
Golgi -2.12 8.41 -1.84 -1.19 -3.35 -6.40 8.32 2.39 10.99 -9.81 -8.70 10.56 -0.67 5.79 -7.10
Lipid Particles 4.41 -1.81 -0.16 -3.87 1.55 -7.79 7.57 -3.30 4.19 -11.04 -7.19 4.83 -4.42 2.19 -8.74
Mitochondria -0.54 5.06 2.88 3.25 -1.99 -12.03 5.70 3.41 16.10 -4.08 -11.73 7.61 4.33 15.91 -4.44
Mitotic Spindle -2.00 -1.58 -4.79 -3.97 -3.12 -2.91 -0.53 -3.67 -1.15 -3.53 -4.05 -1.95 -6.39 -3.63 -4.84
None -0.84 1.69 -0.33 0.43 -1.83 -1.25 4.61 0.66 1.73 -2.96 -1.47 5.40 0.44 1.73 -3.88
Nuclear Periphery 1.55 -10.91 -5.95 -6.68 8.85 -1.41 -13.68 -9.44 -9.23 5.25 -1.21 -17.74 -11.39 -11.26 9.90
Nuclear Periphery Foci -6.19 -13.73 -14.40 -13.48 5.03 -0.94 -21.19 -17.91 -17.60 1.28 -4.13 -25.39 -22.78 -21.94 3.96
Nucleolus 3.60 -0.19 0.10 -1.14 0.19 -2.23 4.43 1.74 4.71 -3.50 -0.92 5.53 1.43 2.19 -2.16
Nucleus 1.83 -12.39 5.67 3.98 15.11 8.47 -16.14 15.65 9.59 32.24 9.01 -20.12 17.02 9.53 33.27
Peroxisomes 0.43 5.90 0.42 0.03 -3.58 -6.97 8.29 2.70 10.95 -6.68 -6.66 10.50 2.13 8.57 -7.47
Vacuole 0.96 -8.00 -19.47 -19.86 -12.07 -13.49 -13.69 -19.81 -11.02 -9.55 -10.47 -14.95 -27.05 -21.20 -15.49
Vacuole Periphery 0.05 3.53 0.40 0.40 -2.05 -11.99 11.09 -0.49 8.97 -5.04 -10.08 8.10 -0.26 8.04 -5.44
Standard name
Human Ortholog
Description Gamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
Localization
Cell Percentages ER (26%), cytoplasm (9%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ost3

Ost3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ost3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available