Standard name
Human Ortholog
Description Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in PM; contains 3 calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact; TCB1 has a paralog, TCB2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.4 0.39 0.55 0.62 0.55 0.54 0.43 0.25 0.41 – 0.41 0.35 0.44 0.29 0.44 0.31 0.17 0.26 0.05 0.08 0.08 0.1 0.16 0.07
Bud 0 0 0.06 0.09 0.12 0.13 0.08 0.08 0.08 – 0.08 0.08 0 0 0.06 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.82 0.79 0.72 0.65 0.53 0.5 0.37 0.25 0.38 – 0.43 0.34 0.82 0.82 0.76 0.55 0.7 0.6 0.91 0.79 0.82 0.75 0.6 0.85
Cytoplasm 0 0.05 0.05 0 0.05 0.06 0.05 0.05 0.06 – 0.06 0.07 0.05 0 0.05 0.14 0.13 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.09 0.06 0 0.1 0.09 0.21 0.19 0.15 – 0.08 0.14 0.07 0 0 0 0 0 0 0 0 0 0.1 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.17 0.09 0 0 0 0 0 0
Mitochondria 0.4 0.3 0.48 0.73 0.6 0.64 0.62 0.61 0.69 – 0.74 0.73 0.54 0.57 0.7 0.07 0.1 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.08 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 104 59 147 222 279 251 302 162 193 – 194 217 105 93 160 99 31 79 13 14 22 15 62 23
Bud 9 3 16 33 62 60 58 54 37 – 39 49 6 11 22 20 5 10 0 1 0 0 8 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 4 5 3 13 5
Bud Site 1 0 1 2 3 1 1 0 0 – 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 214 119 190 234 268 232 261 165 180 – 207 212 196 261 274 173 130 182 261 137 228 122 233 305
Cytoplasm 8 7 14 13 26 28 33 34 26 – 27 41 13 8 17 45 24 55 1 2 1 0 4 2
Endoplasmic Reticulum 11 13 16 8 48 40 147 126 72 – 37 84 17 12 10 14 4 8 3 5 6 6 39 7
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 1 0 0 1 1
Golgi 0 2 0 1 4 1 2 2 1 – 0 5 1 0 0 17 32 26 0 1 0 0 1 1
Mitochondria 105 45 127 261 302 298 430 399 326 – 356 455 129 182 253 21 18 17 2 1 8 6 8 6
Nucleus 0 1 2 0 0 1 0 1 1 – 0 2 1 1 3 0 1 4 0 0 1 0 1 0
Nuclear Periphery 0 0 0 0 0 0 2 9 1 – 8 6 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 2 0 – 2 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 1 0 4 12 38 27 25 14 6 – 9 6 4 8 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 3 2 2 1 – 6 11 0 1 1 1 2 2 1 2 1 2 11 2
Unique Cell Count 260 151 265 358 505 468 698 653 470 478 622 240 318 362 317 187 302 288 175 278 163 391 362
Labelled Cell Count 454 249 517 787 1034 942 1263 970 844 886 1088 472 577 742 391 249 383 288 175 278 163 391 362


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.2 12.6 9.1 8.9 9.4 9.2 9.3 9.5 9.1 – 9.6 8.9 12.6 12.9 11.5 16.3 19.5 18.9 12.6 11.4 13.2
Std Deviation (1e-4) 2.5 2.1 1.3 1.2 1.4 1.5 1.5 1.6 1.7 – 1.8 2.0 1.7 1.9 2.2 4.2 4.8 5.1 2.0 2.3 2.1
Intensity Change (Log2) – – – -0.04 0.05 0.01 0.03 0.05 0.0 – 0.07 -0.03 0.47 0.5 0.33 0.84 1.1 1.05 0.47 0.32 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 -0.1 -0.5 -3.4 -8.9 -3.8 – -3.9 -5.7 -2.6 -6.4 -2.8 -5.9 -8.3 -7.1
Bud 1.5 2.7 2.9 1.2 1.2 0.9 – 1.1 1.0 -1.9 -1.5 0 0.1 -1.7 -1.5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -1.7 -5.0 -5.8 -9.5 -13.1 -8.7 – -7.4 -10.3 2.6 3.0 1.1 -4.2 -0.5 -2.9
Cytoplasm -1.0 -0.1 0.4 -0.4 0 0.1 – 0.2 0.7 0.1 -1.7 -0.3 3.5 2.8 4.7
Endoplasmic Reticulum -2.4 1.7 1.2 5.6 5.0 3.7 – 0.9 3.2 0.5 -1.3 -2.0 -0.9 -2.0 -2.0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 3.8 7.0 4.9
Mitochondria 6.4 3.2 4.2 3.8 3.7 5.7 – 7.3 7.2 1.3 2.2 5.6 -11.4 -8.6 -11.5
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1.4 3.5 2.8 1.7 0.6 0 – 0 0 0 0.9 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.4191 15.8676 15.1063 13.89 13.7914 15.593 10.8451 14.9269 13.5334 13.1496 12.3939 13.7262 7.7346 9.4133 9.0803 8.006 8.5579 8.8336
Actin 0.014 0.0156 0.0103 0.009 0.0029 0.0026 0.0793 0.0057 0.0244 0.0113 0.0138 0.0055 0.0343 0.0094 0.0146 0.0357 0.0013 0.0083
Bud 0.0006 0.0006 0.0028 0.001 0.0004 0.0002 0.004 0.001 0.001 0.0006 0.0011 0.001 0.002 0.0008 0.0009 0.0005 0.0003 0.0004
Bud Neck 0.0059 0.007 0.0057 0.0088 0.0036 0.0096 0.008 0.0071 0.0086 0.0139 0.0086 0.0305 0.0068 0.006 0.0116 0.0274 0.0037 0.0195
Bud Periphery 0.0066 0.0047 0.0281 0.0194 0.01 0.0054 0.0259 0.0044 0.0107 0.0116 0.0126 0.0141 0.0157 0.004 0.0117 0.0102 0.0063 0.0065
Bud Site 0.0263 0.0893 0.0171 0.0017 0.0006 0.0006 0.032 0.1404 0.0372 0.0017 0.0033 0.0011 0.0391 0.1686 0.0415 0.0031 0.0012 0.0016
Cell Periphery 0.7488 0.7587 0.7648 0.744 0.6779 0.8098 0.5198 0.7668 0.7743 0.7904 0.7024 0.8111 0.6972 0.7253 0.825 0.8114 0.8642 0.8293
Cytoplasm 0.007 0.0196 0.0085 0.0379 0.0053 0.004 0.0115 0.0135 0.0127 0.0051 0.0024 0.0046 0.0174 0.0182 0.0113 0.0052 0.005 0.0058
Cytoplasmic Foci 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0197 0.0001 0.0025 0.0014 0.0086 0.0013 0.0013 0.0001 0.0052 0.0007 0.0001 0.0002
Eisosomes 0.1572 0.0728 0.1267 0.1074 0.251 0.1316 0.0584 0.0462 0.0509 0.0729 0.1097 0.0805 0.1145 0.0495 0.0499 0.0666 0.1018 0.0961
Endoplasmic Reticulum 0.0183 0.0165 0.0193 0.0533 0.0348 0.0257 0.0101 0.005 0.0154 0.0371 0.0137 0.0205 0.0173 0.0088 0.0142 0.0133 0.0111 0.0209
Endosome 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.029 0 0.0105 0.0025 0.0014 0.0025 0.0054 0.0001 0.0001 0.0001 0 0.0001
Golgi 0.0004 0.0006 0.0005 0.0025 0.0007 0.0009 0.0184 0.0001 0.0116 0.009 0.0027 0.0051 0.0034 0.0004 0.0005 0.0007 0.0003 0.0005
Lipid Particles 0.001 0.0013 0.0006 0.0015 0.0014 0.0004 0.0207 0.0003 0.0036 0.0045 0.0011 0.0048 0.0034 0.0004 0.0006 0.0009 0.0002 0.0004
Mitochondria 0.0009 0.0017 0.0031 0.0011 0.0033 0.0013 0.0708 0.0006 0.017 0.0235 0.076 0.0068 0.0191 0.0007 0.0014 0.0051 0.0007 0.0046
None 0.0006 0.0006 0.0009 0.0007 0.0012 0.0007 0.0195 0.0004 0.0013 0.0006 0.0016 0.0007 0.0018 0.0006 0.0007 0.0006 0.0007 0.0007
Nuclear Periphery 0.0032 0.0024 0.0025 0.0027 0.0024 0.0014 0.015 0.0019 0.0056 0.0031 0.0023 0.0019 0.0051 0.0026 0.0016 0.0013 0.001 0.0011
Nucleolus 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0014 0.0002 0.0004 0.0004 0.0004 0.0002 0.0013 0.0002 0.0002 0.0002 0.0001 0.0003
Nucleus 0.0003 0.0004 0.0003 0.0003 0.0001 0.0002 0.0162 0.0004 0.0009 0.0004 0.0006 0.0004 0.0038 0.0004 0.0003 0.0002 0.0001 0.0002
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0168 0.0001 0.0015 0.002 0.0018 0.0014 0.0007 0 0.0018 0.0108 0 0.0002
Punctate Nuclear 0 0 0 0 0 0 0.0058 0 0.0012 0.0001 0.0301 0.0008 0.0005 0 0.0004 0 0 0
Vacuole 0.0083 0.0072 0.0079 0.0076 0.0037 0.005 0.0139 0.0057 0.0076 0.0075 0.005 0.005 0.0089 0.0038 0.0062 0.0054 0.0019 0.0032
Vacuole Periphery 0.0002 0.0002 0.0005 0.0004 0.0002 0.0002 0.0037 0.0001 0.001 0.0006 0.0006 0.0006 0.0011 0.0001 0.0002 0.0006 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.6899 45.981 50.9743 61.87 55.6416 53.8854 56.7839 58.504 64.3333 70.2434
Translational Efficiency 0.7841 0.8298 0.7874 0.7946 0.7093 0.8453 0.5884 0.5533 0.5661 0.5761

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in PM; contains 3 calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact; TCB1 has a paralog, TCB2, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (92%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Tcb1

Tcb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tcb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available