Standard name | |
---|---|
Human Ortholog | |
Description | Component of nuclear aminoacylation-dependent tRNA export pathway; cytoplasmic; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a; required for activation of RAN GTPase Gsp1p and dissociation of receptor-tRNA-Gsp1p export complex; recruits Rna1p from cytoplasm to NPC, facilitates Rna1p activation of Gsp1p GTPase activity by enabling Rna1p to gain access to Gsp1p-GTP bound to export receptor tRNA complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 14 | 30 | 3 | 5 | 4 | 11 | 13 | 4 | 3 | 2 | 2 | 4 | 4 | 5 | 11 | 6 | – | 4 | 6 | 4 | 5 | 9 | 9 |
Bud | 3 | 1 | 4 | 2 | 0 | 5 | 3 | 6 | 7 | 14 | 20 | 9 | 3 | 0 | 1 | 0 | 1 | – | 2 | 4 | 1 | 3 | 6 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 3 | 1 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 3 | 3 | 9 | 6 | 4 | 3 | 10 | 10 | 8 | 7 | 2 | 4 | 3 | 9 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 129 | 173 | 178 | 182 | 216 | 186 | 275 | 198 | 206 | 188 | 163 | 213 | 207 | 303 | 307 | 150 | 114 | – | 109 | 175 | 143 | 92 | 169 | 209 |
Endoplasmic Reticulum | 9 | 26 | 21 | 3 | 9 | 11 | 14 | 16 | 4 | 4 | 5 | 5 | 9 | 14 | 22 | 11 | 8 | – | 10 | 12 | 8 | 41 | 80 | 76 |
Endosome | 10 | 3 | 7 | 3 | 9 | 6 | 11 | 3 | 5 | 4 | 7 | 4 | 22 | 12 | 18 | 11 | 6 | – | 23 | 25 | 24 | 19 | 12 | 15 |
Golgi | 9 | 9 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 7 | 2 | – | 21 | 12 | 19 | 25 | 21 | 22 |
Mitochondria | 7 | 3 | 2 | 18 | 10 | 44 | 81 | 35 | 124 | 155 | 161 | 134 | 23 | 12 | 14 | 7 | 0 | – | 7 | 10 | 66 | 39 | 46 | 41 |
Nucleus | 0 | 1 | 1 | 1 | 2 | 1 | 4 | 6 | 0 | 2 | 1 | 2 | 0 | 0 | 2 | 1 | 4 | – | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | – | 5 | 1 | 4 | 2 | 1 | 1 |
Vac/Vac Membrane | 1 | 4 | 3 | 6 | 2 | 5 | 6 | 11 | 13 | 13 | 12 | 17 | 9 | 3 | 10 | 30 | 16 | – | 7 | 18 | 29 | 26 | 33 | 27 |
Unique Cell Count | 164 | 219 | 230 | 201 | 248 | 242 | 369 | 263 | 311 | 324 | 300 | 344 | 259 | 340 | 363 | 214 | 146 | 198 | 276 | 312 | 265 | 394 | 427 | |
Labelled Cell Count | 183 | 239 | 253 | 221 | 263 | 270 | 411 | 293 | 374 | 397 | 381 | 396 | 281 | 357 | 387 | 241 | 159 | 198 | 276 | 312 | 265 | 394 | 427 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 9.9 | 7.6 | 7.0 | 7.4 | 6.1 | 6.1 | 6.2 | 5.5 | 5.4 | 5.0 | 5.4 | 7.7 | 8.0 | 8.3 | 12.0 | 11.7 | – | 7.5 | 7.6 | 7.9 |
Std Deviation (1e-4) | 1.0 | 1.4 | 0.9 | 1.0 | 1.2 | 1.2 | 1.1 | 1.2 | 1.1 | 1.4 | 1.0 | 0.9 | 1.1 | 1.1 | 1.2 | 2.3 | 2.2 | – | 1.2 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.32 | -0.32 | -0.29 | -0.47 | -0.51 | -0.61 | -0.5 | 0.01 | 0.06 | 0.12 | 0.65 | 0.62 | – | -0.02 | -0.0 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -4.5 | -4.6 | -4.8 | -4.7 | -3.2 | -5.6 | -5.9 | -5.9 | -6.4 | -5.0 | -5.9 | -5.9 | -2.9 | -2.9 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 2.7 | 0.7 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 1.6 | 0 | 0 | 0 | 1.4 | 1.4 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | – |
Cytoplasm | 3.7 | 2.8 | -0.1 | -0.8 | -0.5 | -2.8 | -4.7 | -5.5 | -3.9 | 0.7 | 3.8 | 2.2 | -1.7 | 0.2 | – |
Endoplasmic Reticulum | -3.4 | -2.5 | -2.0 | -2.7 | -1.3 | -4.3 | -4.4 | -3.9 | -4.3 | -2.6 | -2.4 | -1.4 | -1.6 | -1.3 | – |
Endosome | 0 | 0.4 | -0.4 | 0 | 0 | -1.1 | 0 | -0.5 | 0 | 2.5 | 0.3 | 1.1 | 1.1 | 0.6 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Mitochondria | 4.0 | 2.2 | 6.3 | 7.3 | 5.2 | 10.6 | 12.1 | 13.1 | 10.5 | 4.0 | 2.0 | 2.2 | 0 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | 0 | 0 | 0 | 0 | 1.9 | 2.0 | 1.9 | 1.9 | 2.3 | 1.5 | 0 | 1.2 | 5.1 | 4.2 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7147 | 3.6176 | 3.1976 | 2.7518 | 2.1909 | 3.278 | 3.7013 | 5.2714 | 4.623 | 4.3105 | 4.439 | 4.6537 | 2.8203 | 4.5036 | 3.5896 | 3.5731 | 3.8389 | 3.9347 |
Actin | 0.0802 | 0.0029 | 0.0134 | 0.0131 | 0.0066 | 0.006 | 0.0439 | 0.0024 | 0.0273 | 0.0232 | 0.0213 | 0.0029 | 0.0194 | 0.0023 | 0.0052 | 0.0018 | 0.0188 | 0.0028 |
Bud | 0.0009 | 0.0008 | 0.0024 | 0.0006 | 0.0004 | 0.0004 | 0.0031 | 0.0023 | 0.0018 | 0.0012 | 0.0024 | 0.0007 | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0005 | 0.0031 |
Bud Neck | 0.0035 | 0.0004 | 0.0008 | 0.0006 | 0.0004 | 0.0035 | 0.0034 | 0.0005 | 0.0015 | 0.0011 | 0.0012 | 0.0015 | 0.0064 | 0.0003 | 0.0007 | 0.001 | 0.0006 | 0.0011 |
Bud Periphery | 0.0009 | 0.0003 | 0.0012 | 0.0003 | 0.0004 | 0.0002 | 0.0036 | 0.0007 | 0.0013 | 0.001 | 0.0017 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0025 |
Bud Site | 0.0039 | 0.0052 | 0.0079 | 0.0008 | 0.0005 | 0.0005 | 0.0057 | 0.0139 | 0.0164 | 0.002 | 0.0214 | 0.0004 | 0.0057 | 0.0028 | 0.001 | 0.0007 | 0.0011 | 0.0003 |
Cell Periphery | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 |
Cytoplasm | 0.3195 | 0.6527 | 0.4998 | 0.3172 | 0.3752 | 0.5058 | 0.4213 | 0.6163 | 0.4117 | 0.3319 | 0.3861 | 0.4759 | 0.4115 | 0.6752 | 0.4393 | 0.3298 | 0.3133 | 0.5446 |
Cytoplasmic Foci | 0.1257 | 0.1072 | 0.1073 | 0.1203 | 0.0919 | 0.1015 | 0.1206 | 0.1119 | 0.1751 | 0.1484 | 0.1631 | 0.1245 | 0.1437 | 0.1166 | 0.1494 | 0.1443 | 0.1388 | 0.1291 |
Eisosomes | 0.0008 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0247 | 0.0122 | 0.0104 | 0.0129 | 0.0091 | 0.0151 | 0.0072 | 0.0077 | 0.005 | 0.0204 | 0.0025 | 0.0159 | 0.0117 | 0.0073 | 0.0067 | 0.0084 | 0.0066 | 0.0064 |
Endosome | 0.2578 | 0.1645 | 0.2734 | 0.4344 | 0.3907 | 0.2775 | 0.256 | 0.1906 | 0.261 | 0.3294 | 0.2994 | 0.287 | 0.2402 | 0.1494 | 0.2873 | 0.4279 | 0.3802 | 0.2455 |
Golgi | 0.052 | 0.0241 | 0.0332 | 0.0292 | 0.0559 | 0.0475 | 0.067 | 0.0381 | 0.0586 | 0.0641 | 0.0417 | 0.066 | 0.0512 | 0.0299 | 0.0351 | 0.0415 | 0.0466 | 0.0361 |
Lipid Particles | 0.0258 | 0.0016 | 0.0038 | 0.0029 | 0.0191 | 0.0007 | 0.0102 | 0.0008 | 0.0032 | 0.0123 | 0.0104 | 0.0004 | 0.0177 | 0.0003 | 0.0051 | 0.0052 | 0.0017 | 0.0043 |
Mitochondria | 0.0171 | 0.0039 | 0.0049 | 0.0083 | 0.0146 | 0.0066 | 0.0165 | 0.0041 | 0.0161 | 0.0106 | 0.0078 | 0.0128 | 0.0139 | 0.0024 | 0.0232 | 0.0026 | 0.0053 | 0.0097 |
None | 0.0137 | 0.0086 | 0.0166 | 0.0029 | 0.0018 | 0.0194 | 0.0222 | 0.0019 | 0.0016 | 0.0017 | 0.0012 | 0.0014 | 0.0326 | 0.0031 | 0.0084 | 0.0028 | 0.0033 | 0.0014 |
Nuclear Periphery | 0.0074 | 0.0015 | 0.0047 | 0.005 | 0.0077 | 0.0014 | 0.001 | 0.0006 | 0.0009 | 0.0027 | 0.0013 | 0.0008 | 0.0076 | 0.0007 | 0.0032 | 0.0016 | 0.0413 | 0.0015 |
Nucleolus | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0028 | 0.0008 | 0.0016 | 0.0042 | 0.0012 | 0.0012 | 0.0006 | 0.0007 | 0.0006 | 0.0012 | 0.0009 | 0.0006 | 0.0018 | 0.0005 | 0.0013 | 0.0006 | 0.0051 | 0.0005 |
Peroxisomes | 0.0223 | 0.001 | 0.0037 | 0.0284 | 0.0025 | 0.001 | 0.0069 | 0.0014 | 0.0085 | 0.0293 | 0.0195 | 0.0018 | 0.0178 | 0.0023 | 0.02 | 0.0051 | 0.0229 | 0.0043 |
Punctate Nuclear | 0.0256 | 0.0007 | 0.0011 | 0.0039 | 0.0015 | 0.0005 | 0.0007 | 0.0003 | 0.0006 | 0.0008 | 0.002 | 0.0003 | 0.0076 | 0.0002 | 0.0038 | 0.0004 | 0.0014 | 0.0003 |
Vacuole | 0.011 | 0.0079 | 0.0094 | 0.0094 | 0.0119 | 0.0075 | 0.0066 | 0.0041 | 0.0057 | 0.0124 | 0.0115 | 0.0038 | 0.0059 | 0.0042 | 0.0065 | 0.0193 | 0.007 | 0.0035 |
Vacuole Periphery | 0.0034 | 0.0032 | 0.0037 | 0.0054 | 0.0083 | 0.0034 | 0.0023 | 0.0015 | 0.0025 | 0.0058 | 0.004 | 0.0029 | 0.0029 | 0.0015 | 0.0028 | 0.006 | 0.005 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.754 | 18.1224 | 15.6889 | 19.4273 | 26.91 | 32.3216 | 19.8505 | 18.698 | 19.4435 | 24.9944 |
Translational Efficiency | 0.8738 | 1.0008 | 0.9 | 0.8504 | 0.696 | 0.6811 | 1.0 | 0.8728 | 0.8037 | 0.6611 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1271 | 99 | 450 | 1252 | 1468 | 1748 | 1544 | 131 | 2739 | 1847 | 1994 | 1383 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 865.17 | 979.35 | 1294.80 | 1272.01 | 875.35 | 974.75 | 1218.87 | 1293.38 | 870.63 | 975.00 | 1236.01 | 1274.03 |
Standard Deviation | 110.12 | 159.03 | 157.99 | 186.54 | 115.65 | 128.70 | 185.40 | 169.96 | 113.23 | 130.51 | 182.36 | 185.14 |
Intensity Change Log 2 | — | 0.178841 | 0.581674 | 0.556054 | — | 0.155172 | 0.477612 | 0.563214 | — | 0.166986 | 0.530277 | 0.559660 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000719 | 0.002275 | 0.004820 | 0.001372 | 0.000614 | 0.001318 | 0.000849 | 0.000777 | 0.000663 | 0.001370 | 0.001745 | 0.001315 |
Bud Neck | 0.009783 | 0.002774 | 0.003981 | 0.002574 | 0.002691 | 0.002516 | 0.002525 | 0.003629 | 0.005982 | 0.002530 | 0.002853 | 0.002674 |
Bud Site | 0.024665 | 0.053048 | 0.073691 | 0.055834 | 0.026334 | 0.029665 | 0.033832 | 0.072379 | 0.025559 | 0.030918 | 0.042827 | 0.057401 |
Cell Periphery | 0.000209 | 0.000335 | 0.000319 | 0.000410 | 0.000154 | 0.000081 | 0.000092 | 0.000219 | 0.000180 | 0.000094 | 0.000144 | 0.000391 |
Cytoplasm | 0.136090* | 0.110790* | 0.186651* | 0.719948* | 0.194332* | 0.160739* | 0.489387* | 0.379699* | 0.167305* | 0.158061* | 0.421066* | 0.687719* |
Cytoplasmic Foci | 0.413211* | 0.479461* | 0.315468* | 0.099176 | 0.491155* | 0.482602* | 0.230358* | 0.273168* | 0.454986* | 0.482433* | 0.249565* | 0.115657 |
Eisosomes | 0.000099 | 0.000126 | 0.000025 | 0.000012 | 0.000088 | 0.000066 | 0.000012 | 0.000013 | 0.000093 | 0.000069 | 0.000015 | 0.000012 |
Endoplasmic Reticulum | 0.004096 | 0.004896 | 0.002742 | 0.000352 | 0.002667 | 0.001700 | 0.001647 | 0.000493 | 0.003330 | 0.001871 | 0.001894 | 0.000365 |
Endosome | 0.134721 | 0.132420 | 0.095010 | 0.011886 | 0.088810 | 0.077228 | 0.048092 | 0.074095 | 0.110114 | 0.080186 | 0.058680 | 0.017779 |
Golgi | 0.159155* | 0.142799* | 0.100103 | 0.015231 | 0.131434* | 0.197661* | 0.022905 | 0.012817 | 0.144298* | 0.194720* | 0.040327 | 0.015002 |
Lipid Particles | 0.016265 | 0.010549 | 0.008408 | 0.004460 | 0.004490 | 0.001736 | 0.003939 | 0.005414 | 0.009954 | 0.002208 | 0.004947 | 0.004550 |
Mitochondria | 0.017334 | 0.002843 | 0.000185 | 0.002066 | 0.008461 | 0.006546 | 0.000613 | 0.000025 | 0.012579 | 0.006347 | 0.000516 | 0.001872 |
Mitotic Spindle | 0.002595 | 0.005992 | 0.102141* | 0.006697 | 0.002796 | 0.007184 | 0.018444 | 0.004938 | 0.002703 | 0.007120 | 0.037333 | 0.006530 |
None | 0.002839 | 0.000985 | 0.000090 | 0.000099 | 0.000168 | 0.000074 | 0.000250 | 0.000039 | 0.001407 | 0.000123 | 0.000214 | 0.000093 |
Nuclear Periphery | 0.000361 | 0.000068 | 0.000242 | 0.000062 | 0.000054 | 0.000021 | 0.000190 | 0.000043 | 0.000197 | 0.000023 | 0.000202 | 0.000060 |
Nuclear Periphery Foci | 0.000678 | 0.000458 | 0.007083 | 0.001046 | 0.000540 | 0.000123 | 0.002284 | 0.001987 | 0.000604 | 0.000141 | 0.003367 | 0.001135 |
Nucleolus | 0.001836 | 0.000309 | 0.000049 | 0.000147 | 0.000241 | 0.000051 | 0.000200 | 0.000089 | 0.000981 | 0.000065 | 0.000166 | 0.000142 |
Nucleus | 0.006853 | 0.001246 | 0.002017 | 0.002568 | 0.001309 | 0.000404 | 0.003919 | 0.001586 | 0.003882 | 0.000449 | 0.003489 | 0.002475 |
Peroxisomes | 0.007380 | 0.007787 | 0.004210 | 0.003769 | 0.003441 | 0.005511 | 0.002751 | 0.005315 | 0.005269 | 0.005633 | 0.003080 | 0.003915 |
Vacuole | 0.044846 | 0.030280 | 0.083259 | 0.069139 | 0.026505 | 0.014519 | 0.130260 | 0.156254 | 0.035016 | 0.015364 | 0.119653 | 0.077390 |
Vacuole Periphery | 0.016265 | 0.010562 | 0.009505 | 0.003153 | 0.013714 | 0.010257 | 0.007452 | 0.007021 | 0.014898 | 0.010273 | 0.007916 | 0.003519 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.56 | -5.21 | -3.15 | 0.98 | 4.46 | -2.88 | -1.23 | -0.81 | 1.82 | 0.23 | -2.94 | -4.65 | -4.20 | 0.48 | 1.91 |
Bud Neck | 6.78 | 5.57 | 7.37 | 0.29 | 4.26 | 0.61 | 0.38 | -0.82 | -0.98 | -0.93 | 7.01 | 6.14 | 6.46 | -0.77 | 0.76 |
Bud Site | -2.92 | -10.03 | -9.44 | 0.10 | 4.09 | -1.79 | -3.22 | -4.60 | -4.23 | -3.86 | -3.15 | -8.55 | -10.86 | -8.19 | -3.97 |
Cell Periphery | -0.94 | -1.90 | -2.64 | -0.28 | -0.97 | 3.12 | 2.71 | -1.38 | -3.87 | -3.58 | 3.28 | 1.44 | -3.98 | -6.95 | -5.65 |
Cytoplasm | 1.70 | -4.60 | -63.91* | -38.93 | -38.68 | 4.20 | -29.53 | -7.38 | -8.71 | 4.00 | 1.37 | -30.59 | -60.72 | -58.54 | -24.45 |
Cytoplasmic Foci | -3.16 | 9.91 | 45.66 | 19.05 | 21.71 | 1.23 | 36.57 | 12.34 | 11.98 | -1.92 | -4.53 | 35.20 | 61.41 | 62.03 | 21.67 |
Eisosomes | -0.50 | 13.30 | 16.98 | 2.20 | 6.18 | 1.01 | 3.53 | 3.44 | 9.60 | -0.76 | 1.89 | 6.64 | 6.92 | 10.30 | 2.86 |
Endoplasmic Reticulum | -0.24 | 1.70 | 5.31 | 1.36 | 8.73 | 1.41 | 2.74 | 6.58 | 1.96 | 4.27 | 2.05 | 3.52 | 8.03 | 2.53 | 7.58 |
Endosome | 0.13 | 6.28 | 24.24 | 9.02 | 19.02 | 3.06 | 12.09 | 2.19 | 0.95 | -2.53 | 8.08 | 15.78 | 30.00 | 24.23 | 18.65 |
Golgi | 0.91 | 7.40 | 26.32 | 7.73 | 11.59 | -11.09 | 27.23 | 27.14 | 36.79* | 3.81 | -9.28 | 28.33 | 36.63 | 38.68 | 9.87 |
Lipid Particles | 1.00 | 4.29 | 7.09 | 1.05 | 4.97 | 7.59 | 1.07 | -0.87 | -2.98 | -1.23 | 9.23 | 5.98 | 6.08 | -4.72 | 0.90 |
Mitochondria | 7.21 | 11.35 | 9.43 | 0.77 | -2.28 | 1.35 | 6.15 | 6.71 | 10.25 | 2.18 | 5.41 | 12.15 | 9.56 | 5.57 | -1.68 |
Mitotic Spindle | -1.30 | -11.53 | -2.78 | -0.10 | 10.98 | -4.46 | -9.00 | -1.09 | 0.80 | 4.82 | -4.90 | -13.98 | -2.88 | 0.66 | 11.42 |
None | 1.58 | 3.19 | 3.17 | 1.06 | -0.41 | 4.62 | -1.05 | 5.80 | 2.25 | 2.97 | 3.18 | 2.98 | 3.27 | 0.59 | 2.05 |
Nuclear Periphery | 3.09 | 1.03 | 3.13 | 0.07 | 4.93 | 3.59 | -6.59 | -0.05 | -3.25 | 6.43 | 4.04 | -0.47 | 2.75 | -1.98 | 5.51 |
Nuclear Periphery Foci | 1.44 | -6.12 | -3.19 | -4.24 | 5.64 | 2.34 | -5.26 | -1.90 | -2.49 | -0.14 | 4.07 | -8.39 | -3.49 | -8.45 | 6.31 |
Nucleolus | 3.00 | 3.94 | 3.69 | 0.61 | -1.54 | 1.21 | 0.67 | 1.08 | -1.72 | 4.07 | 3.91 | 3.54 | 3.51 | -1.31 | 0.22 |
Nucleus | 4.45 | 4.08 | 3.21 | -3.04 | -2.65 | 5.63 | -8.84 | -1.02 | -3.43 | 5.18 | 6.80 | 0.16 | 1.21 | -14.91 | 1.83 |
Peroxisomes | -0.15 | 5.29 | 7.56 | 2.36 | 1.30 | -4.66 | 2.25 | -2.03 | 0.66 | -3.14 | -0.67 | 7.13 | 4.74 | 4.72 | -1.68 |
Vacuole | 1.79 | -7.00 | -9.14 | -6.91 | 0.74 | 7.28 | -22.97 | -8.64 | -9.36 | -2.07 | 10.66 | -22.08 | -15.34 | -21.70 | 6.09 |
Vacuole Periphery | 2.39 | 4.56 | 12.41 | 3.65 | 5.25 | 3.45 | 7.28 | 3.78 | 2.05 | 0.35 | 5.15 | 9.31 | 16.28 | 9.81 | 7.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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