Standard name
Human Ortholog
Description Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0.06 0.08 0.05 0.08 0.07 0.05 0.06 0 0 0.09 0.13 0.12 0.1 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.32 0 0.09 0 0.25 0.2 0.16 0.46 0.43 0.53 0.31 0.08 0 0 0.05 0 0.13 0 0 0 0 0 0
Nucleus 0.85 0.87 0.92 0.94 0.91 0.82 0.84 0.83 0.78 0.72 0.74 0.83 0.9 0.85 0.85 0.85 0.84 0.82 0.76 0.79 0.79 0.6 0.49 0.42
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0.05 0.06 0.13 0.14 0.15 0.16 0 0 0.06 0 0 0 0.09 0.06 0.08 0.12 0.12 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.15 0.17 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2
Bud 0 4 0 0 0 0 3 3 4 5 4 7 0 0 0 0 0 1 1 4 0 3 12 17
Bud Neck 1 2 7 0 3 1 0 5 1 1 1 3 0 0 0 0 0 0 0 3 1 0 4 10
Bud Site 0 0 0 0 2 5 7 1 9 4 12 10 1 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 15 10 9 11 15 15 23 22 13 15 10 1 20 38 41 32 33 35 1 3 1 1 4 7
Endoplasmic Reticulum 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 6 8 10 1 0 0 4 3 2
Endosome 1 2 0 0 0 0 0 2 0 0 0 0 0 1 3 1 4 2 4 4 0 5 13 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 2 0 1 4 3
Mitochondria 15 169 11 16 7 70 61 52 124 109 122 63 19 7 10 17 9 37 2 6 3 6 10 9
Nucleus 186 462 274 173 169 227 255 273 211 180 170 166 207 251 278 266 275 236 174 460 256 150 188 142
Nuclear Periphery 0 0 1 0 1 2 2 0 1 0 1 2 0 1 0 2 0 2 1 0 1 0 1 1
Nucleolus 12 1 1 2 0 11 15 20 35 34 34 33 7 6 21 4 8 2 21 37 24 29 46 35
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 1 0
SpindlePole 5 17 21 5 12 9 10 10 3 4 2 4 0 5 5 9 9 7 3 12 3 5 16 18
Vac/Vac Membrane 5 10 2 0 2 2 1 2 0 0 0 1 2 0 2 7 9 7 8 41 26 37 67 66
Unique Cell Count 220 529 297 185 186 277 305 328 269 251 230 201 230 294 328 314 328 287 229 583 323 252 387 342
Labelled Cell Count 241 680 326 207 211 342 377 390 401 352 356 290 256 310 360 346 357 341 229 583 323 252 387 342


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.4 5.6 5.2 5.9 4.8 4.9 5.4 4.4 4.4 4.1 4.5 5.5 5.4 5.5 7.3 7.6 6.9 4.9 4.9 5.1
Std Deviation (1e-4) 0.9 0.6 1.1 1.0 1.4 1.4 1.3 1.5 1.1 1.3 1.0 0.9 1.7 1.7 1.4 1.5 1.7 1.6 1.8 1.8 1.8
Intensity Change (Log2) – – – -0.1 0.08 -0.21 -0.2 -0.05 -0.33 -0.35 -0.44 -0.32 -0.01 -0.04 -0.02 0.38 0.45 0.3 -0.17 -0.2 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1648 2.7922 2.6392 2.4884 2.209 2.5582 2.3904 1.827 1.963 1.5186 0.9385 1.4911 2.481 1.6874 1.6518 1.7032 1.7748 1.6995
Actin 0.0365 0 0.0021 0.0713 0.0167 0.0111 0.0351 0 0.0206 0.0043 0 0.0003 0.0083 0 0.0031 0 0.0083 0.0016
Bud 0.0003 0.0007 0.0001 0.0001 0.0036 0.0001 0.0006 0 0.0003 0.0003 0 0 0.0004 0 0.0002 0 0.0006 0
Bud Neck 0.0062 0.0005 0.0006 0.0019 0.0071 0.0021 0.0021 0.0001 0.0003 0.0032 0.0002 0.0006 0.0051 0.0018 0.0008 0 0.0012 0.0005
Bud Periphery 0.0004 0.0001 0.0005 0.0002 0.0099 0.0002 0.0013 0 0.0005 0.0019 0 0.0001 0.001 0 0.0005 0 0.0012 0.0002
Bud Site 0.0037 0.0025 0.003 0.0002 0.0064 0.0002 0.0022 0 0.002 0.0007 0 0.0001 0.0022 0.0007 0.0013 0 0.0015 0.0001
Cell Periphery 0.0003 0.0001 0.0004 0.0001 0.0005 0.0001 0.0004 0 0.0001 0.0002 0 0 0.0003 0 0.0001 0 0.0004 0
Cytoplasm 0.0042 0.0081 0.0004 0.0019 0.0026 0.0001 0.0047 0.0001 0.0007 0.0004 0 0.0001 0.0029 0 0.0004 0 0.0013 0.0003
Cytoplasmic Foci 0.0144 0.0003 0.0047 0.0004 0.0048 0.0002 0.0074 0 0.0087 0.0012 0 0.0017 0.0045 0 0.0008 0 0.0025 0.001
Eisosomes 0.0003 0 0.0001 0.0005 0.0005 0.0002 0.0003 0 0.0004 0.0002 0 0 0.0002 0 0 0 0.0002 0.0001
Endoplasmic Reticulum 0.005 0.0001 0.0001 0.0002 0.0053 0.0003 0.0067 0 0.0002 0.0012 0 0.0001 0.0021 0 0.0005 0 0.0014 0.0004
Endosome 0.0098 0.0001 0.0016 0.0007 0.0193 0.0006 0.0168 0 0.0026 0.0072 0 0.0029 0.0143 0 0.0034 0 0.0127 0.0037
Golgi 0.0086 0 0.0011 0.0018 0.0053 0.0011 0.0063 0 0.0022 0.003 0 0.0023 0.0032 0 0.0009 0 0.0066 0.0011
Lipid Particles 0.018 0 0.0117 0.0003 0.0314 0.0009 0.0074 0 0.0034 0.0225 0 0.0038 0.0122 0 0.0024 0 0.0263 0.0076
Mitochondria 0.0019 0.0001 0.0095 0.0002 0.0433 0.0006 0.0065 0.0001 0.0091 0.0059 0 0.006 0.0162 0 0.0024 0 0.0345 0.0017
None 0.017 0.0005 0.0007 0.003 0.0033 0.0002 0.0124 0 0.0025 0.0005 0 0.0002 0.0022 0 0.0004 0 0.0008 0.0022
Nuclear Periphery 0.0037 0.0035 0.0008 0.0081 0.0432 0.0005 0.0186 0.0007 0.0031 0.0272 0.0002 0.001 0.0171 0.0005 0.0041 0.0007 0.0189 0.0066
Nucleolus 0.0192 0.0501 0.0218 0.0348 0.0856 0.0651 0.022 0.0163 0.0174 0.0143 0.0627 0.0453 0.03 0.0238 0.0157 0.0134 0.1067 0.0494
Nucleus 0.8199 0.9137 0.9175 0.8253 0.6251 0.9095 0.8298 0.9782 0.9045 0.8926 0.935 0.9292 0.8448 0.9398 0.9367 0.9777 0.7034 0.9041
Peroxisomes 0.0231 0 0.0187 0.0002 0.0064 0.0003 0.0037 0 0.0116 0.0014 0 0.0006 0.0021 0 0.0006 0 0.0024 0.0007
Punctate Nuclear 0.0065 0.0195 0.0028 0.0488 0.0712 0.0066 0.0069 0.0045 0.0094 0.0076 0.0019 0.0052 0.0257 0.0331 0.0245 0.0081 0.0647 0.0176
Vacuole 0.0006 0.0002 0.0008 0.0001 0.0045 0.0001 0.0069 0 0.0002 0.002 0 0.0002 0.0035 0 0.0007 0 0.0023 0.0003
Vacuole Periphery 0.0003 0 0.001 0.0001 0.004 0.0001 0.0019 0 0.0002 0.0024 0 0.0002 0.002 0 0.0004 0 0.0019 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8384 7.1867 8.5326 13.9939 8.8599 12.0524 11.0814 15.4488 13.1202 15.5578
Translational Efficiency 1.2353 1.0286 1.0539 0.6275 1.0055 1.1785 0.964 0.751 1.0876 0.9125

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1084 1537 139 1460 1396 2584 1419 108 2480 4121 1558 1568

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 583.08 769.38 1030.64 913.33 680.05 756.63 848.32 911.90 637.66 761.39 864.59 913.23
Standard Deviation 64.49 110.69 134.01 133.26 82.06 101.81 113.40 148.94 89.01 105.39 126.56 134.40
Intensity Change Log 2 — 0.400002 0.821775 0.647442 — 0.153947 0.318968 0.423235 — 0.272761 0.572931 0.531075

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000072 0.000508 0.001829 0.001761 0.000221 0.000239 0.000675 0.001355 0.000156 0.000339 0.000778 0.001733
Bud Neck 0.001840 0.008578 0.011307 0.054329 0.003598 0.011384 0.012714 0.010864 0.002830 0.010338 0.012589 0.051335
Bud Site 0.000389 0.001291 0.001635 0.019050 0.001435 0.001760 0.001357 0.003264 0.000978 0.001585 0.001382 0.017963
Cell Periphery 0.000105 0.000084 0.000092 0.000176 0.000091 0.000070 0.000058 0.000179 0.000098 0.000075 0.000061 0.000177
Cytoplasm 0.034242 0.012991 0.000672 0.014807 0.018048 0.008352 0.001785 0.006510 0.025126 0.010082 0.001686 0.014236
Cytoplasmic Foci 0.003980 0.001244 0.000046 0.002965 0.003621 0.001646 0.000087 0.000117 0.003778 0.001496 0.000083 0.002769
Eisosomes 0.000021 0.000023 0.000065 0.000033 0.000026 0.000020 0.000029 0.000035 0.000024 0.000021 0.000032 0.000033
Endoplasmic Reticulum 0.000993 0.003045 0.006813 0.006345 0.001667 0.001986 0.003896 0.005093 0.001372 0.002381 0.004156 0.006259
Endosome 0.000409 0.000517 0.000218 0.004392 0.000423 0.001324 0.000450 0.000378 0.000417 0.001023 0.000429 0.004115
Golgi 0.000143 0.000495 0.000141 0.003584 0.000489 0.000857 0.000153 0.000147 0.000338 0.000722 0.000151 0.003347
Lipid Particles 0.001858 0.000378 0.000169 0.000234 0.001824 0.000737 0.000108 0.000157 0.001839 0.000603 0.000113 0.000229
Mitochondria 0.000883 0.001938 0.001275 0.007165 0.003127 0.005950 0.001081 0.001577 0.002146 0.004454 0.001099 0.006780
Mitotic Spindle 0.000473 0.003733 0.000271 0.056312 0.002089 0.009010 0.001796 0.030904 0.001383 0.007042 0.001660 0.054562
None 0.034149 0.007156 0.001772 0.005323 0.011855 0.004861 0.003820 0.007989 0.021599 0.005717 0.003637 0.005506
Nuclear Periphery 0.000465 0.000761 0.000217 0.002907 0.000752 0.001628 0.000410 0.000456 0.000626 0.001305 0.000393 0.002738
Nuclear Periphery Foci 0.000316 0.000535 0.000187 0.002312 0.000817 0.000549 0.000133 0.000185 0.000598 0.000544 0.000138 0.002165
Nucleolus 0.027786 0.035640 0.026415 0.011424 0.029742 0.034103 0.019218 0.013349 0.028887 0.034676 0.019861 0.011556
Nucleus 0.890046* 0.919288* 0.943791* 0.791625* 0.917674* 0.911646* 0.950849* 0.909720* 0.905598* 0.914496* 0.950219* 0.799759*
Peroxisomes 0.000783 0.000479 0.000969 0.000615 0.001427 0.000833 0.000178 0.000354 0.001146 0.000701 0.000249 0.000597
Vacuole 0.000761 0.000953 0.001945 0.009947 0.000764 0.001972 0.001037 0.007167 0.000763 0.001592 0.001118 0.009756
Vacuole Periphery 0.000285 0.000363 0.000171 0.004695 0.000310 0.001074 0.000166 0.000199 0.000299 0.000809 0.000166 0.004385

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.14 -9.42 -7.79 -5.43 1.25 -0.26 -13.66 -6.17 -6.15 -4.74 -6.58 -19.38 -8.26 -7.27 -3.96
Bud Neck -8.67 -8.17 -21.43 -18.25 -14.24 -8.71 -11.11 -7.70 -4.35 -2.30 -12.17 -13.58 -21.69 -18.06 -14.81
Bud Site -3.66 -5.41 -7.46 -6.60 -5.82 -0.46 -0.67 -2.36 -2.30 -2.28 -1.55 -2.27 -7.22 -6.85 -6.70
Cell Periphery 0.88 0.50 -1.10 -2.26 -1.18 1.75 2.70 -2.10 -2.67 -2.90 1.96 3.53 -2.38 -4.74 -6.80
Cytoplasm 6.02 10.20 7.43 1.99 -5.24 4.61 8.50 4.40 0.93 -2.62 7.77 13.31 7.72 0.92 -5.96
Cytoplasmic Foci 3.81 5.48 4.33 1.09 -2.89 3.34 5.57 5.42 5.61 -1.19 5.00 7.79 5.88 2.09 -2.86
Eisosomes -0.82 -8.03 -10.31 -9.86 4.71 4.66 -2.74 -5.22 -6.37 -4.62 3.63 -6.97 -9.81 -14.86 -3.38
Endoplasmic Reticulum -4.69 -8.26 -11.12 -10.45 0.12 -1.84 -14.64 -6.79 -6.54 -4.20 -4.72 -13.83 -15.52 -14.39 -5.91
Endosome -0.65 1.12 -3.34 -3.02 -3.80 -3.10 0.07 -2.15 1.67 -1.67 -3.13 0.10 -3.46 -1.64 -3.29
Golgi -1.24 -0.49 -2.45 -1.45 -2.40 -0.88 1.74 0.86 1.58 -2.62 -1.43 1.62 -1.93 -1.02 -2.46
Lipid Particles 2.28 2.65 2.62 2.92 -0.42 2.77 4.54 3.70 2.04 -3.35 3.45 4.96 4.71 4.33 -2.58
Mitochondria -3.40 -3.36 -7.91 -5.36 -6.98 -2.60 2.24 0.36 3.60 -3.35 -3.47 1.77 -4.19 -0.54 -7.38
Mitotic Spindle -2.92 -0.51 -9.60 -8.26 -9.54 -4.05 -0.27 -2.44 -2.00 -2.42 -5.08 -1.16 -9.60 -7.83 -9.27
None 9.77 11.84 10.78 3.51 -5.24 5.08 6.48 3.16 -0.85 -2.07 10.94 12.88 11.59 1.66 -3.80
Nuclear Periphery -2.21 1.23 -8.77 -7.75 -10.23 -3.61 1.25 -2.36 0.87 -2.97 -4.25 0.25 -8.78 -4.97 -9.07
Nuclear Periphery Foci -2.04 0.59 -2.07 -0.88 -2.43 1.46 2.87 1.68 0.37 -1.74 0.35 2.70 -0.86 -1.12 -2.44
Nucleolus -2.12 0.79 7.21 10.84 2.64 -1.75 5.22 1.94 2.96 -0.77 -2.69 5.83 9.85 14.68 3.38
Nucleus -4.49 -6.21 5.07 10.57 11.25 0.65 -5.96 3.25 3.11 4.67 -2.53 -9.96 8.71 11.22 16.52
Peroxisomes 1.16 -0.32 1.80 1.90 4.91 1.39 2.83 1.43 0.24 -3.31 1.67 3.35 2.85 3.65 -1.94
Vacuole -0.51 -3.07 -7.84 -7.56 -4.22 -2.06 -2.79 -2.59 -2.49 -2.48 -2.08 -3.62 -7.83 -7.07 -6.61
Vacuole Periphery -0.39 0.48 -2.50 -2.38 -2.75 -2.45 1.17 -0.17 2.55 -2.74 -2.42 1.28 -2.53 -1.48 -2.83
Standard name
Human Ortholog
Description Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Azf1

Azf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Azf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available