Standard name
Human Ortholog
Description RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.05 0.05 0 0 0 0 0 0 0.05 0 0.07 0.14 0.09 0.06 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.07 0.05 0.15 0.16 0.19 0.34 0.31 0.26 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.84 0.83 0.92 0.9 0.87 0.81 0.79 0.75 0.69 0.74 0.72 0.86 0.83 0.74 0.81 0.72 0.73 0.73 0.79 0.78 0.8 0.65 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.3 0.25 0.26 0.09 0.08 0.15 0.2 0.3 0.27 0.26 0.28 0.3 0.25 0.08 0.25 0.19 0.23 0.21 0.25 0.13 0.13 0.14 0.15 0.23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 1 1 3 1 0 1 1 0 1 0 0 0 0 1 2 1 0 2 0
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 2 1 7 5 12 8 10 1 13 0 0 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 13 6 8 10 7 8 6 4 9 8 6 9 25 18 14 12 22 0 1 0 0 2 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 0 0 0 0 0
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 1 1 1 2 0 0 2 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 1
Mitochondria 0 5 9 11 14 37 60 85 94 93 43 90 0 0 6 8 4 4 0 1 1 0 1 2
Nucleus 404 219 104 155 245 207 298 351 205 206 122 181 105 151 141 190 137 190 346 224 105 84 125 81
Nuclear Periphery 0 1 0 2 2 5 7 6 4 11 7 9 0 0 1 1 0 1 0 0 0 0 0 0
Nucleolus 136 65 33 16 22 36 73 133 73 79 47 76 31 14 47 44 43 56 118 35 17 14 28 38
Peroxisomes 0 1 0 0 0 0 0 0 0 1 0 1 0 0 3 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2 0 0 6 0 0 2 2 1 0 0 1 1
Vac/Vac Membrane 3 3 0 2 6 0 1 2 1 0 0 0 0 2 5 7 8 11 3 11 4 2 23 38
Unique Cell Count 460 262 125 169 273 239 368 446 274 300 165 252 122 181 190 236 191 262 477 284 135 106 193 166
Labelled Cell Count 548 309 153 196 300 300 453 599 390 410 229 379 145 194 236 268 209 289 477 284 135 106 193 166


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.3 15.2 9.1 9.5 8.3 6.9 6.2 5.8 5.1 5.3 5.3 4.7 15.8 15.5 12.4 16.8 18.6 20.1 15.0 15.9 17.9
Std Deviation (1e-4) 4.1 6.3 2.2 2.4 1.9 1.7 1.7 1.5 1.3 1.5 1.8 1.5 5.7 5.5 4.8 6.6 6.1 7.9 4.0 7.1 7.1
Intensity Change (Log2) – – – 0.07 -0.13 -0.39 -0.55 -0.66 -0.85 -0.79 -0.78 -0.96 0.8 0.77 0.45 0.88 1.03 1.15 0.72 0.81 0.98


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.5 0 0 0 0 0 0 0 0.8 2.6 1.5 0.4 0.6 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 -0.8 2.3 2.5 3.2 5.7 5.2 4.1 0 0 0 -1.6 -1.6 -2.2 0
Nucleus 2.2 1.8 0.9 -0.6 -1.1 -1.9 -3.1 -1.9 -2.4 0.6 0.1 -1.9 -0.6 -2.3 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.9 -4.9 -2.6 -1.5 0.7 0.1 0 0.4 0 -0.2 -4.5 -0.3 -1.7 -0.8 -1.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4052 1.3333 1.6171 1.0563 0.9344 1.5113 1.9028 1.9105 2.0298 1.6419 1.5594 1.66 0.0525 0.4555 0.1213 0.1147 0.9645 0.0828
Actin 0.0326 0.0001 0.0083 0.0001 0.0001 0.0015 0.0264 0.0001 0.0128 0.024 0.0253 0.0008 0.0978 0.0046 0.009 0 0.0163 0.0004
Bud 0.0006 0.0001 0.0001 0 0.0002 0.0001 0.0008 0.0004 0.0016 0.0002 0.0003 0.0001 0.0003 0.0001 0.0002 0 0.0006 0.0001
Bud Neck 0.013 0.0008 0.0009 0.0002 0.0006 0.004 0.0147 0.0003 0.0012 0.0005 0.0006 0.0016 0.0003 0.0007 0.0006 0.0001 0.0009 0.0033
Bud Periphery 0.0021 0.0002 0.0001 0 0.0006 0.0002 0.0019 0.0002 0.0005 0.0003 0.0005 0.0003 0.0004 0.0001 0.0002 0 0.0009 0.0002
Bud Site 0.0127 0.001 0.0004 0 0.0002 0.0004 0.0044 0.0009 0.007 0.001 0.0002 0.0002 0.0008 0.0006 0.0039 0 0.0019 0.0007
Cell Periphery 0.0041 0.0001 0 0 0.0002 0.0001 0.0009 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.0054 0.0124 0.0032 0.001 0.0011 0.0027 0.0047 0.0121 0.0042 0.0003 0.0373 0.0009 0.0064 0.0241 0.0044 0.0002 0.0099 0.0043
Cytoplasmic Foci 0.0047 0.0008 0.0083 0 0.0005 0.003 0.0143 0.0045 0.0148 0.0076 0.0011 0.0019 0.0094 0.0156 0.0031 0 0.0252 0.0035
Eisosomes 0.001 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0044 0.0006 0.0004 0.0001 0.0011 0.0003 0.0012 0.0004 0.0005 0.0001 0.0017 0.0003 0.0003 0.0005 0.0003 0 0.0012 0.0002
Endosome 0.0075 0.0023 0.0047 0.0003 0.0234 0.0028 0.0396 0.0041 0.0107 0.0014 0.0048 0.0048 0.0043 0.0108 0.0011 0 0.0183 0.0008
Golgi 0.0028 0.0004 0.0036 0 0.0018 0.0017 0.0178 0.0005 0.012 0.0025 0.008 0.0026 0.0102 0.0169 0.0022 0 0.0231 0.0002
Lipid Particles 0.0084 0.0007 0.0085 0.0001 0.0019 0.0076 0.0275 0.0001 0.0033 0.0029 0.0004 0.0045 0.0054 0.0356 0.0016 0 0.0088 0.0015
Mitochondria 0.0086 0.0005 0.0008 0.0003 0.0093 0.001 0.0175 0.0085 0.0047 0.0039 0.0101 0.0029 0.002 0.0025 0.0023 0.0001 0.057 0.0003
None 0.0208 0.0171 0.013 0.001 0.0006 0.0024 0.0222 0.0097 0.0098 0.0003 0.0075 0.0007 0.003 0.0033 0.015 0.0001 0.1288 0.0188
Nuclear Periphery 0.0092 0.007 0.0082 0.0262 0.0132 0.0036 0.0059 0.0072 0.0028 0.0006 0.0041 0.0028 0.0045 0.0048 0.0309 0.001 0.0129 0.0037
Nucleolus 0.0305 0.0348 0.0296 0.0148 0.0277 0.0446 0.0177 0.0183 0.0227 0.0133 0.0381 0.0258 0.0073 0.0245 0.014 0.0072 0.0191 0.0288
Nucleus 0.7914 0.8985 0.8776 0.9487 0.8635 0.903 0.7416 0.9079 0.846 0.9135 0.844 0.9064 0.7922 0.817 0.8738 0.9901 0.6303 0.892
Peroxisomes 0.0139 0.0001 0.003 0 0.0001 0.0024 0.0119 0.0003 0.0145 0.0262 0.0002 0.0007 0.0344 0.0124 0.0042 0 0.0261 0.0067
Punctate Nuclear 0.0217 0.0206 0.0286 0.0065 0.032 0.0175 0.007 0.0238 0.0301 0.0013 0.0149 0.0415 0.0205 0.0251 0.0314 0.0011 0.0169 0.0338
Vacuole 0.0035 0.0015 0.0004 0.0002 0.016 0.0008 0.0169 0.0006 0.0005 0.0001 0.0003 0.0005 0.0001 0.0005 0.0004 0 0.0012 0.0006
Vacuole Periphery 0.0009 0.0003 0.0003 0.0003 0.0059 0.0002 0.0049 0.0001 0.0002 0.0001 0.0003 0.0005 0.0001 0.0002 0.0011 0 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.2303 66.8386 70.6384 68.6206 57.4464 50.573 63.3709 59.127 58.3206 61.781
Translational Efficiency 0.6986 0.6561 0.6296 0.5688 0.562 0.6101 0.5524 0.4529 0.4496 0.4809

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
128 1050 173 1529 2096 2329 147 1738 2224 3379 320 3267

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 599.29 757.63 1061.75 856.59 704.93 735.69 972.64 830.06 698.85 742.51 1020.82 842.48
Standard Deviation 95.13 98.34 112.49 114.39 78.35 92.16 88.32 107.67 83.14 94.67 111.34 111.66
Intensity Change Log 2 — 0.338239 0.825118 0.515351 — 0.061618 0.464426 0.235736 — 0.195336 0.641409 0.370973

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000337 0.000801 0.002467 0.001281 0.000120 0.000198 0.001028 0.000662 0.000132 0.000385 0.001806 0.000951
Bud Neck 0.011940 0.008250 0.005731 0.016713 0.004675 0.010390 0.002326 0.013651 0.005093 0.009725 0.004166 0.015084
Bud Site 0.001717 0.003553 0.002976 0.029788 0.001082 0.002841 0.001695 0.010564 0.001118 0.003062 0.002388 0.019561
Cell Periphery 0.000153 0.000137 0.000199 0.000194 0.000068 0.000098 0.000070 0.000164 0.000073 0.000110 0.000140 0.000178
Cytoplasm 0.114725* 0.071245 0.008571 0.112538* 0.044654 0.060804 0.009469 0.098426 0.048687 0.064048 0.008984 0.105030*
Cytoplasmic Foci 0.023695 0.024199 0.001154 0.006074 0.008731 0.012868 0.000448 0.004457 0.009592 0.016389 0.000830 0.005214
Eisosomes 0.000065 0.000061 0.000127 0.000030 0.000028 0.000035 0.000076 0.000030 0.000030 0.000043 0.000103 0.000030
Endoplasmic Reticulum 0.001739 0.004122 0.019336 0.006031 0.001497 0.002694 0.009274 0.005095 0.001511 0.003138 0.014714 0.005533
Endosome 0.001650 0.003860 0.001635 0.008098 0.000729 0.002612 0.000642 0.006025 0.000782 0.003000 0.001179 0.006995
Golgi 0.000294 0.001952 0.000229 0.006638 0.000449 0.000866 0.000091 0.003674 0.000440 0.001203 0.000166 0.005061
Lipid Particles 0.004845 0.007004 0.002468 0.000932 0.003921 0.004435 0.001121 0.000962 0.003974 0.005233 0.001849 0.000948
Mitochondria 0.001840 0.009915 0.001210 0.007792 0.005346 0.013834 0.000886 0.010537 0.005144 0.012617 0.001061 0.009252
Mitotic Spindle 0.000892 0.015366 0.007872 0.041671 0.002721 0.015646 0.000306 0.034987 0.002615 0.015559 0.004396 0.038115
None 0.016025 0.014895 0.001802 0.010974 0.009302 0.008166 0.005708 0.018113 0.009689 0.010257 0.003597 0.014772
Nuclear Periphery 0.002084 0.002976 0.001462 0.003853 0.003855 0.005053 0.001027 0.003029 0.003753 0.004408 0.001262 0.003415
Nuclear Periphery Foci 0.004641 0.003051 0.001757 0.001448 0.001238 0.001470 0.002059 0.001101 0.001434 0.001961 0.001896 0.001263
Nucleolus 0.033220 0.030851 0.009563 0.002466 0.029552 0.025811 0.006165 0.003158 0.029763 0.027377 0.008002 0.002834
Nucleus 0.775795* 0.784902* 0.902087* 0.714367* 0.879373* 0.820643* 0.943795* 0.761015* 0.873412* 0.809537* 0.921247* 0.739183*
Peroxisomes 0.001642 0.008008 0.000862 0.001593 0.001438 0.003039 0.000373 0.000817 0.001450 0.004583 0.000637 0.001180
Vacuole 0.002452 0.004169 0.028174 0.024093 0.000956 0.007066 0.013218 0.021075 0.001042 0.006166 0.021304 0.022488
Vacuole Periphery 0.000250 0.000684 0.000318 0.003425 0.000265 0.001430 0.000222 0.002459 0.000264 0.001198 0.000274 0.002911

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.69 -9.31 -3.41 -1.30 8.22 -3.85 -7.78 -10.18 -8.87 3.37 -9.25 -14.67 -14.21 -8.69 8.44
Bud Neck 0.57 0.42 -1.06 -7.90 -7.13 -5.98 -0.68 -11.67 -4.53 -9.74 -5.63 -3.06 -13.90 -8.01 -11.36
Bud Site -2.13 -2.90 -5.99 -5.01 -4.88 -2.85 -2.13 -6.10 -4.38 -4.37 -3.83 -4.53 -8.73 -6.76 -6.44
Cell Periphery 0.43 -1.40 0.73 0.65 3.56 -3.90 -2.80 -5.46 -2.45 -1.63 -5.74 -7.23 -7.46 -2.26 2.17
Cytoplasm 2.11 5.89 1.49 -1.97 -19.50 -4.47 6.22 -7.97 -4.02 -11.08 -4.69 11.42 -9.19 -4.81 -20.30
Cytoplasmic Foci -0.57 4.46 4.18 9.41 -2.52 -2.62 9.91 6.73 9.43 -4.41 -4.70 10.56 8.22 13.48 -4.89
Eisosomes 0.18 -4.93 2.04 4.09 8.65 -2.57 -6.16 -5.97 -3.58 4.93 -5.89 -11.38 -7.31 -0.91 9.47
Endoplasmic Reticulum -2.48 -12.62 -10.02 -13.02 7.89 -4.57 -9.90 -20.42 -15.00 4.04 -7.83 -17.13 -27.73 -20.31 8.69
Endosome -2.13 -0.21 -1.51 0.97 -1.99 -5.44 -1.02 -4.23 -0.75 -3.42 -6.47 -3.33 -5.85 -0.15 -3.72
Golgi -3.49 0.57 -2.53 0.32 -2.77 -1.25 1.58 -1.54 -0.75 -2.27 -2.53 1.22 -2.54 -0.69 -3.51
Lipid Particles -1.70 2.64 4.14 8.15 4.25 -0.84 5.14 7.04 8.76 2.17 -2.17 4.77 7.57 11.95 4.69
Mitochondria -5.84 1.84 -2.24 4.22 -3.57 -5.75 5.40 0.10 5.26 -4.01 -6.13 5.23 0.63 7.07 -5.18
Mitotic Spindle -5.32 -0.97 -9.43 -4.51 -4.53 -6.00 0.81 -7.75 -2.93 -7.88 -7.54 -0.64 -11.86 -5.44 -6.97
None 0.33 4.98 3.09 5.93 -8.74 1.27 3.19 -0.15 -1.09 -2.96 -0.66 6.89 1.40 2.29 -5.84
Nuclear Periphery -0.22 1.19 -5.63 -7.74 -12.18 -1.66 3.89 -1.90 0.03 -8.15 -1.01 3.80 -3.93 -3.48 -14.17
Nuclear Periphery Foci 0.80 1.19 1.35 2.64 1.00 0.69 -0.31 1.36 0.88 0.79 -0.41 -0.37 1.20 2.21 1.29
Nucleolus 0.79 3.50 5.13 13.10 3.08 2.43 12.01 17.22 16.35 1.60 1.74 9.67 18.29 21.00 3.42
Nucleus -0.20 -3.98 0.79 2.19 9.09 7.82 -4.21 10.74 3.49 10.56 9.83 -3.04 14.94 5.16 13.28
Peroxisomes -5.55 2.48 2.56 6.71 0.78 -3.62 5.78 5.47 6.66 -0.21 -6.51 5.21 4.96 9.21 0.79
Vacuole -1.32 -5.83 -16.33 -16.44 -1.69 -6.86 -4.25 -15.88 -12.97 -2.87 -8.15 -7.44 -23.81 -20.47 -2.74
Vacuole Periphery -4.96 -1.87 -2.54 -1.06 -2.21 -4.78 0.42 -3.17 1.18 -3.19 -5.51 -1.01 -4.02 0.70 -3.77
Standard name
Human Ortholog
Description RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rpo31

Rpo31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpo31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available