Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.94 0.93 0.96 0.92 0.96 0.96 – 0.93 0.98 0.88 0.92 0.96 0.95 0.98 0.91 0.91 0.86 0.44 0.48 0.41 0.48 0.31 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.06 0.1 0.05 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0.21 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.08 0.06 0 0.08 0 0 – 0 0 0 0 0.09 0.06 0 0.15 0.1 0.18 0.36 0.38 0.42 0.35 0.56 0.54
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 2 – 1 0 1 2 0 0 0 0 1 0 0 0 2 0 0 0
Bud 0 1 0 1 1 0 1 – 3 1 2 8 0 0 0 0 0 0 0 0 2 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 0 1 1 1 – 1 7 1 7 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 113 171 167 186 237 358 400 – 186 277 281 324 54 123 160 143 166 130 64 91 81 16 19 24
Endoplasmic Reticulum 4 7 6 4 2 2 1 – 3 2 1 3 1 1 1 4 6 2 5 3 7 0 0 0
Endosome 4 0 2 2 5 2 4 – 0 0 3 2 0 1 1 3 0 0 13 14 11 3 3 3
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 1 0 2 0 0 0
Mitochondria 3 0 0 0 0 5 8 – 9 11 66 41 0 0 0 1 0 0 2 0 4 1 0 1
Nucleus 0 0 3 0 3 3 1 – 1 4 0 3 0 2 0 0 0 3 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 14 10 7 21 12 17 – 6 4 10 8 5 8 5 23 19 27 52 73 83 11 33 44
Unique Cell Count 129 182 180 194 259 372 418 201 284 320 353 56 129 163 158 183 151 146 192 200 34 61 83
Labelled Cell Count 132 193 192 200 270 385 437 210 306 366 399 60 135 167 175 192 164 146 192 200 34 61 83


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 8.7 7.5 7.4 7.9 6.4 6.5 – 5.5 5.8 5.1 5.5 7.9 7.6 7.8 10.2 11.3 10.6 – 8.0 8.6
Std Deviation (1e-4) 0.7 1.2 0.9 1.2 1.5 1.1 1.4 – 1.1 1.1 0.9 0.9 1.3 1.1 1.1 1.7 2.1 2.7 – 2.0 1.7
Intensity Change (Log2) – – – -0.02 0.07 -0.24 -0.21 – -0.46 -0.37 -0.57 -0.47 0.07 0.01 0.04 0.43 0.58 0.49 – 0.09 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 -0.5 1.8 1.5 – -0.1 2.5 -1.7 -0.4 1.0 0.9 2.4 -0.8 -0.7 -2.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 6.5 4.8 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 1.0 -1.3 -0.8 – -1.2 -2.5 -1.3 -2.0 0 0.2 -1.1 2.8 1.7 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5369 3.1527 2.7898 2.2889 2.5593 2.729 2.0392 2.8905 2.2689 2.3506 1.9923 2.4106 2.4567 2.9426 2.7463 2.5945 2.3272 2.5777
Actin 0.0155 0.0002 0.0123 0.0273 0.0362 0.0048 0.0161 0 0.004 0.0866 0.021 0.0016 0.0253 0.0001 0.0031 0.0438 0.0138 0.0036
Bud 0.0005 0.0012 0.0008 0.0054 0.0035 0.0011 0.0008 0.0003 0.001 0.0018 0.0026 0.0005 0.0004 0.0008 0.0008 0.0157 0.0004 0.001
Bud Neck 0.0021 0.0003 0.0021 0.0064 0.0016 0.0061 0.0133 0.0002 0.0008 0.002 0.0006 0.0021 0.001 0.0011 0.0006 0.0012 0.0018 0.0011
Bud Periphery 0.0006 0.0008 0.0007 0.0038 0.0032 0.0012 0.001 0.0003 0.0008 0.0033 0.0078 0.0003 0.0004 0.0003 0.0005 0.0068 0.0006 0.0005
Bud Site 0.0022 0.0019 0.0045 0.0161 0.056 0.0008 0.0094 0.0013 0.0091 0.0057 0.0036 0.0005 0.0022 0.006 0.0022 0.0072 0.0017 0.0018
Cell Periphery 0.0004 0.0002 0.0002 0.0002 0.0004 0.0001 0.0006 0.0002 0.0003 0.0005 0.0005 0.0001 0.0002 0.0003 0.0002 0.0002 0.0002 0.0001
Cytoplasm 0.5807 0.7636 0.6782 0.4884 0.4859 0.7053 0.4792 0.6687 0.6203 0.5034 0.5526 0.7392 0.6446 0.8026 0.7196 0.5439 0.6365 0.8051
Cytoplasmic Foci 0.0405 0.0235 0.0377 0.0635 0.0192 0.0428 0.0641 0.0396 0.0367 0.0696 0.0355 0.0305 0.0244 0.0164 0.0197 0.0349 0.0535 0.0189
Eisosomes 0.0002 0 0.0001 0.0003 0.0002 0.0001 0.0002 0 0.0001 0.0002 0.0005 0 0.0002 0 0 0.0002 0.0002 0
Endoplasmic Reticulum 0.0154 0.0134 0.0092 0.008 0.0046 0.0105 0.0221 0.0083 0.0123 0.0066 0.0058 0.0123 0.023 0.0292 0.0104 0.0056 0.009 0.0141
Endosome 0.103 0.0799 0.0998 0.1353 0.1046 0.0986 0.1347 0.1111 0.13 0.184 0.1694 0.1036 0.0884 0.0426 0.0905 0.1094 0.1091 0.0735
Golgi 0.0082 0.0015 0.0051 0.0174 0.0173 0.0024 0.0105 0.0015 0.003 0.0055 0.0108 0.0054 0.0064 0.0005 0.0012 0.0049 0.0116 0.001
Lipid Particles 0.0139 0.0004 0.0039 0.0086 0.0087 0.0005 0.0111 0.0015 0.0081 0.0011 0.0162 0.0028 0.0067 0.0004 0.0007 0.0027 0.0341 0.0009
Mitochondria 0.0068 0.0006 0.0125 0.0136 0.007 0.004 0.0041 0.001 0.0022 0.0038 0.0472 0.0023 0.0022 0.0003 0.0004 0.0021 0.0036 0.0005
None 0.0984 0.0432 0.0606 0.0608 0.0808 0.063 0.1003 0.1088 0.0573 0.0738 0.013 0.0269 0.0341 0.0157 0.0302 0.0657 0.0224 0.0098
Nuclear Periphery 0.0091 0.0074 0.0057 0.0063 0.0112 0.0054 0.0091 0.0025 0.0037 0.0023 0.0047 0.0044 0.0193 0.0084 0.0055 0.0031 0.0083 0.0042
Nucleolus 0.0006 0.0001 0.0005 0.0007 0.0004 0.0002 0.0012 0.0005 0.0008 0.0002 0.0003 0.0001 0.0006 0.0002 0.0002 0.0002 0.0009 0.0001
Nucleus 0.0069 0.0057 0.0056 0.0053 0.0092 0.0047 0.0037 0.0026 0.0038 0.0022 0.0024 0.0037 0.0078 0.0034 0.0037 0.0028 0.0043 0.0036
Peroxisomes 0.0157 0.0001 0.0069 0.0147 0.004 0.0014 0.0091 0.0001 0.01 0.0011 0.0107 0.0018 0.0077 0.0001 0.0004 0.0029 0.0179 0.0072
Punctate Nuclear 0.0041 0.0006 0.0028 0.0066 0.0149 0.0013 0.0059 0.0007 0.0016 0.0006 0.0006 0.0008 0.0062 0.0006 0.001 0.0009 0.0127 0.0006
Vacuole 0.0696 0.0491 0.0447 0.0985 0.1137 0.0383 0.0942 0.0438 0.0816 0.0392 0.0769 0.0497 0.0926 0.0675 0.0978 0.1321 0.0506 0.044
Vacuole Periphery 0.0057 0.0065 0.0062 0.013 0.0174 0.0074 0.0095 0.007 0.0127 0.0067 0.0172 0.0115 0.0064 0.0034 0.0112 0.0137 0.0067 0.0085

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.3319 5.7886 8.1612 16.0565 15.5319 12.2964 14.0468 17.9737 13.1969 16.6623
Translational Efficiency 0.9357 1.2267 1.105 0.5281 0.6429 0.9184 0.5432 0.5169 0.7231 0.7549

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity
Localization
Cell Percentages cytoplasm (79%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rtc5

Rtc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available