Standard name
Human Ortholog
Description Glycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; human homolog AKR1B1 can complement yeast null mutant; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.91 0.99 0.99 0.98 0.97 0.91 0.87 0.81 0.75 0.74 0.72 0.72 0.98 0.97 0.95 0.97 0.96 0.97 0.77 0.91 0.86 0.9 0.85 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0 0 0.09 0.17 0.17 0.32 0.23 0.39 0.23 0 0 0 0 0 0 0.14 0 0.05 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.07 0.07 0.1 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 2 0 1 1 1 1 0 0 0 0 0 0 1 2 4
Bud 0 1 0 0 1 3 7 6 9 9 9 13 2 1 1 0 0 0 1 0 1 1 9 7
Bud Neck 1 0 1 5 11 2 6 7 1 3 1 4 6 0 2 1 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 2 2 1 4 4 3 3 3 0 1 1 2 5 12 0 0 0 2 3 4
Cytoplasm 164 140 102 263 443 367 518 469 301 294 262 283 390 482 463 192 157 206 133 131 101 215 403 433
Endoplasmic Reticulum 1 0 0 1 0 2 1 4 1 1 0 3 5 2 0 9 4 12 0 0 2 0 2 2
Endosome 0 0 0 1 0 0 2 0 1 0 0 0 0 0 0 1 1 0 1 0 0 1 3 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0 0 1
Mitochondria 33 3 0 8 8 38 101 100 129 92 144 91 0 1 0 3 5 2 23 5 6 7 21 18
Nucleus 0 0 1 1 1 3 6 11 14 16 10 10 1 0 4 0 4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 4 1 2 2 5 3 6 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 3 4 1 1 1 4 0 4 5 0 0 1 1 2 1 0 7 8
Vac/Vac Membrane 1 0 0 0 7 11 32 41 28 41 19 45 1 7 15 3 3 5 2 0 0 1 3 6
Unique Cell Count 181 142 103 268 457 405 595 579 400 399 366 392 397 495 487 198 164 212 173 144 118 240 477 515
Labelled Cell Count 201 144 105 282 475 433 679 649 491 467 453 463 406 501 492 212 180 238 173 144 118 240 477 515


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.7 4.4 4.8 5.1 4.3 4.3 5.2 4.4 5.1 4.7 5.4 5.2 7.3 9.7 8.3 8.5 7.5 4.4 4.7 5.0
Std Deviation (1e-4) 0.4 1.2 1.1 3.0 2.0 1.7 1.8 2.2 2.0 2.4 2.3 3.0 1.7 4.8 7.0 4.1 5.2 3.7 1.2 1.1 1.2
Intensity Change (Log2) – – – 0.13 0.22 -0.03 -0.01 0.24 0.0 0.23 0.11 0.3 0.26 0.74 1.15 0.92 0.96 0.78 0.02 0.11 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4652 2.8805 1.6559 0.8109 0.3916 1.9454 2.6975 3.5523 2.6985 0.8425 2.4171 2.6546 0.9828 1.7064 1.3597 2.051 1.0879 2.505
Actin 0.0155 0.0015 0.0017 0.0003 0.0008 0.0009 0.0227 0.0019 0.0078 0.0382 0.0057 0.0098 0.0334 0.016 0.0055 0.0039 0.0121 0.0019
Bud 0.0074 0.017 0.0125 0.0076 0.0042 0.0027 0.0047 0.0065 0.0136 0.0085 0.0005 0.0012 0.0053 0.029 0.0083 0.0112 0.0152 0.0018
Bud Neck 0.0024 0.0007 0.0065 0.0005 0.0006 0.0013 0.0017 0.0004 0.0013 0.0049 0.0006 0.0015 0.0088 0.0004 0.0039 0.0097 0.0016 0.0035
Bud Periphery 0.0045 0.0079 0.0101 0.0085 0.0059 0.0021 0.0128 0.0079 0.0139 0.0125 0.0008 0.0009 0.0017 0.0069 0.0043 0.007 0.0092 0.0011
Bud Site 0.0028 0.0195 0.0112 0.0007 0.0003 0.0002 0.0141 0.0169 0.0187 0.0011 0.0006 0.0003 0.0062 0.0249 0.0063 0.0021 0.0014 0.0002
Cell Periphery 0.0004 0.0005 0.0005 0.0003 0.0004 0.0001 0.0013 0.0009 0.0007 0.0005 0.0001 0.0001 0.0003 0.001 0.0008 0.0003 0.0006 0.0001
Cytoplasm 0.4172 0.5382 0.3947 0.3659 0.4497 0.5244 0.4098 0.5304 0.5626 0.4232 0.5934 0.6699 0.5282 0.5646 0.6564 0.7239 0.549 0.7258
Cytoplasmic Foci 0.0197 0.008 0.0071 0.0059 0.0051 0.0212 0.0614 0.0101 0.0184 0.0093 0.0239 0.0105 0.0306 0.0097 0.0091 0.021 0.0192 0.033
Eisosomes 0.0001 0.0001 0.0001 0 0.0001 0 0.0012 0.0001 0.0002 0.0004 0 0.0002 0.0013 0.0001 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0054 0.0026 0.0024 0.0018 0.0048 0.0021 0.0047 0.0016 0.0057 0.0047 0.0049 0.0055 0.0055 0.0012 0.003 0.0062 0.0045 0.0025
Endosome 0.0108 0.0029 0.0018 0.0015 0.0034 0.0035 0.0481 0.0048 0.0121 0.0219 0.048 0.0149 0.0276 0.0017 0.0041 0.005 0.059 0.008
Golgi 0.0023 0.0002 0.0002 0.0001 0.0001 0.0002 0.0132 0.003 0.0042 0.0163 0.0292 0.0046 0.0091 0.001 0.0007 0.0008 0.0038 0.0006
Lipid Particles 0.0025 0.0002 0.0002 0.0001 0.0002 0.0001 0.0184 0.0013 0.0069 0.0225 0.002 0.0024 0.0073 0.0006 0.0035 0.001 0.0037 0.001
Mitochondria 0.0017 0.0007 0.001 0.0007 0.0004 0.0005 0.0099 0.0024 0.0053 0.0538 0.0111 0.003 0.0029 0.0012 0.0043 0.0007 0.0027 0.0005
None 0.4731 0.3725 0.5144 0.5865 0.5038 0.4084 0.3309 0.4025 0.3008 0.3631 0.2252 0.2554 0.2853 0.317 0.2683 0.1863 0.2643 0.1991
Nuclear Periphery 0.0073 0.0035 0.0031 0.0026 0.0041 0.0031 0.0039 0.0007 0.0044 0.0021 0.0021 0.0034 0.0108 0.0016 0.0029 0.0035 0.0295 0.0027
Nucleolus 0.0005 0.0005 0.0008 0.0006 0.0002 0.0004 0.001 0.0003 0.0008 0.0003 0.0001 0.0001 0.0006 0.0005 0.0008 0.0003 0.0006 0.0004
Nucleus 0.0098 0.0157 0.0238 0.0103 0.0058 0.017 0.0051 0.0036 0.0085 0.006 0.0038 0.0103 0.0103 0.0077 0.0084 0.0072 0.0087 0.0076
Peroxisomes 0.0071 0.0004 0.0003 0.0002 0.0001 0.0008 0.0201 0.0018 0.0065 0.0049 0.0407 0.0005 0.0066 0.0087 0.0017 0.0034 0.0024 0.0011
Punctate Nuclear 0.0044 0.0045 0.005 0.0024 0.0009 0.009 0.0097 0.0012 0.0016 0.0015 0.0008 0.0026 0.0149 0.004 0.0045 0.0018 0.0046 0.0071
Vacuole 0.0043 0.0028 0.0022 0.0029 0.0078 0.0016 0.0039 0.0015 0.0048 0.0031 0.0043 0.0022 0.0025 0.0019 0.0023 0.0042 0.0052 0.0017
Vacuole Periphery 0.0008 0.0004 0.0004 0.0005 0.0012 0.0004 0.0013 0.0002 0.001 0.0014 0.002 0.0007 0.0007 0.0003 0.0005 0.0007 0.0026 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7508 7.3532 10.9127 14.6744 6.856 11.7443 14.8696 18.9966 26.3806 10.7454
Translational Efficiency 1.2726 0.451 0.3641 0.959 0.5699 2.7312 0.567 1.0776 1.2634 0.907

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; human homolog AKR1B1 can complement yeast null mutant; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (27%), nucleus (11%), mixed (34%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gcy1

Gcy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available